Online citations, reference lists, and bibliographies.
← Back to Search

Decreased Insulin Binding To Adipocytes And Circulating Monocytes From Obese Subjects.

J. Olefsky
Published 1976 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
UNLABELLED Insulin binding to isolated adipocytes from 16 normal and 14 obese patients was studied. The data indicated that, as a group, adipocytes from the obese patients bound significantly less insulin than normal. However, of the 14 obese patients, 5 were not hyperinsulinemic and 4 of these 5 subjects had normal insulin binding. These subjects were also younger, and had the onset of obesity in childhood. When these five patients were separated from the original 14 obese patients, enhanced differences in insulin binding to adipocytes were observed when normals and the remaining 9 obese subjects were compared. Similar findings were obtained with isolated circulating mononuclear cells from these same patients. Presumably the five normoinsulinemic obese patients were not insulin-resistant, and, thus, the data indicate that insulin binding to adipocytes was decreased only in insulin-resistant obese patients. This conclusion was strengthened by finding a highly significant correlation (r=-0.71, p less than 0.001) between insulin binding to adipocytes and fasting plasma insulin level, while a weaker correlation (r=-0.49,p less than 0.01) existed between insulin binding and degree of obesity. Finally, when insulin binding to adipocytes and mononuclear cells from the same individual was compared, a significant positive correlation was found (r=0.53,p less than 0.01). IN CONCLUSION (a) insulin binding to adipocytes and mononuclear cells is decreased in cells from insulin-resistant obese patients; (b) a significant inverse relationship exists between fasting plasma insulin level and insulin binding to adipocytes; and (c) in obesity, events that affect insulin receptors on adipocytes similarly affect insulin receptors on mononuclear cells.
This paper references
10.1152/AJPLEGACY.1971.221.3.850
A simple method to determine fat cell size and number in four mammalian species.
M. di Girolamo (1971)
10.1210/JCEM-36-4-627
Insulin receptors in human circulating lymphocytes: application to the study of insulin resistance in man.
J. A. Archer (1973)
Thymic lymphocytes in obese (ob-ob) mice. A mirror of the insulin receptor defect in liver and fat.
A. Soll (1974)
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.
M. Rodbell (1964)
10.1126/SCIENCE.164059
Insulin receptor: role in the resistance of human obesity to insulin.
J. Amatruda (1975)
10.2337/diab.22.7.507
Relationship Between Fasting Plasma Insulin Level and Resistance to Insulin-Mediated Glucose Uptake in Normal and Diabetic Subjects
J. Olefsky (1973)
10.1038/194495A0
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W. Hunter (1962)
10.1210/JCEM-33-5-732
Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays.
B. Desbuquois (1971)
10.1210/JCEM-38-4-554
The human lymphocyte: a model for the study of insulin-receptor interaction.
J. Olefsky (1974)
10.1016/S0006-291X(73)80072-5
Insulin interactions with its receptors: experimental evidence for negative cooperativity.
P. De Meyts (1973)
10.1172/JCI107878
Decreased insulin binding to lymphocytes from diabetic subjects.
J. Olefsky (1974)
A one-stage procedure for isolation of granulocytes and lymphocytes from human blood. General sedimentation properties of white blood cells in a 1g gravity field.
A. Böyum (1968)
10.1016/0014-5793(72)80519-2
Impairment of insulin binding to the fat cell plasma membrane in the obese hyperglycemic mouse
P. Freychet (1972)
10.1172/JCI108360
The effects of spontaneous obesity on insulin binding, glucose transport, and glucose oxidation of isolated rat adipocytes.
J. Olefsky (1976)
10.2337/diab.23.7.565
Insulin Binding to Isolated Human Adipocytes
J. Olefsky (1974)
10.1016/0006-291X(75)90473-8
Cooperativity in ligand binding: a new graphic analysis.
P. De Meyts (1975)
Identification and quantitation of thymus-derived lymphocytes in human peripheral blood.
A. Bobrove (1974)
10.1073/PNAS.69.3.747
Insulin receptors in human circulating cells and fibroblasts.
J. Gavin (1972)
10.1073/PNAS.71.1.84
Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture.
J. Gavin (1974)
Characteristics of the human lymphocyte insulin receptor.
J. Gavin (1973)
10.1111/j.1749-6632.1949.tb27297.x
THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS
G. Scatchard (1949)
10.1210/ENDO-96-6-1486
Effects of Age and Obesity on Insulin Binding to Isolated Adipocytes1212
J. Olefsky (1975)
10.1016/0006-291X(71)90767-4
Monoiodoinsulin: demonstration of its biological activity and binding to fat cells and liver membranes.
P. Freychet (1971)
Insulin-induced reduction of membrane receptor concentrations in isolated fat cells and lymphocytes. Independence from receptor occupation and possible relation to proteolytic activity of insulin.
D. Huang (1975)
Methods for the determination of adipose cell size in man and animals.
J. Hirsch (1968)
10.1093/AJCP/55.3.283
Cytochemical identification of monocytes and granulocytes.
L. Yam (1971)
Insulin-receptor interaction in the obese-hyperglycemic mouse. A model of insulin resistance.
C. Kahn (1973)
10.1073/PNAS.72.2.474
Demonstration that monocytes rather than lymphocytes are the insulin-binding cells in preparations of humah peripheral blood mononuclear leukocytes: implications for studies of insulin-resistant states in man.
R. Schwartz (1975)



This paper is referenced by
10.1007/978-94-009-5822-7_7
Insulin Receptors in Disorders of Glucose Tolerance and Insulin Sensitivity
E. Obberghen (1981)
10.1172/JCI112789
Decreased kinase activity of insulin receptors from adipocytes of non-insulin-dependent diabetic subjects.
G. Freidenberg (1987)
10.1111/dom.13006
LAPSInsulin115: A novel ultra‐long‐acting basal insulin with a unique action profile
Nina Wronkowitz (2017)
Differential distribution of adipose tissue in South Asian and European populations
S. Kohli (2007)
10.2337/diab.28.8.731
Effect of a High Carbohydrate Diet on Insulin Binding to Adipocytes and on Insulin Action in vivo in Man
O. Kolterman (1979)
10.2337/diab.26.7.657
In-vitro Observations on Isolated Adipose Tissue Cells from Hyperobese Subjects
T. Burns (1977)
10.3181/00379727-193-43017
Diabetogenic Response to Streptozotocin Varies among Obese Yellow and Among Lean Agouti (BALB/c × VY)F1 Hybrid Mice
George L. Wolff (1990)
10.1172/JCI110908
Mechanisms of insulin resistance in aging.
R. Fink (1983)
10.1210/JCEM-48-3-487
Relationship between in vivo insulin resistance and decreased insulin receptors in obese man.
O. Kolterman (1979)
10.1007/BF00423149
Receptor binding and effects of insulin and NSILA-S on glucose transport and metabolism in adipocytes from hypophysectomized rats
E. Schoenle (2004)
10.1016/S0022-3476(84)81090-2
The insulin receptor.
S. Kaplan (1984)
10.1016/0026-0495(84)90224-5
Diminished insulin receptors on monocytes and erythrocytes in hypertriglyceridemia.
W. Bieger (1984)
10.1111/j.1365-2265.1984.tb01411.x
THE EFFECTS OF CYPROTERONE ACETATE AND ETHINYL OESTRADIOL ON CARBOHYDRATE METABOLISM
M. Seed (1984)
10.1172/JCI110350
Receptor and postreceptor defects contribute to the insulin resistance in noninsulin-dependent diabetes mellitus.
O. Kolterman (1981)
10.1007/978-1-4757-3906-0_4
Mechanisms of Insulin Resistance in Obesity
D. Kelley (1997)
10.1016/S0026-0495(97)90206-7
Improved sensitivity to insulin in obese subjects following weight loss is accompanied by reduced protein-tyrosine phosphatases in adipose tissue.
F. Ahmad (1997)
10.1007/BF03348136
Insulin receptors on circulating blood cells from patients with pancreatogenic diabetes: a comparison with type I diabetes and normal subjects
M. Muggeo (1987)
10.1210/JCEM-60-2-311
Insulin-stimulated glucose disposal is not increased in anorexia nervosa.
M. Castillo (1985)
10.1172/JCI108618
Increased insulin binding by hepatic plasma membranes from diabetic rats: normalization by insulin therapy.
M. Davidson (1977)
Diabetes and Cardiovascular Disease in Migrant South Asians
N. Abate ()
10.1016/0006-2952(91)90073-E
Reduced insulin binding to liver plasma membranes in inherently obese diabetic CBA/Ca mice.
P. Taberner (1991)
10.1016/0014-5793(89)81674-6
Insulin induces a similar reduction in the concentrations of its own receptor and of an insulin‐sensitive glycosyl‐phosphatidylinositol in isolated rat hepatocytes
J. M. Ruiz-Albusac (1989)
10.1155/S0962935193000250
Hormonal control of inflammatory responses
J. Garcia-Leme (1993)
10.1002/IJC.2910350309
Insulin binding by normal and neoplastic colon tissue
M. Wong (1985)
10.1016/S0165-7208(02)80023-X
Discovery of small molecule insulin mimetics as potential novel antidiabetic agents
B. Zhang (2002)
10.1007/BF03348218
Response of insulin receptors to oral glucose in normal subjects
A. Bertoli (1980)
10.1007/978-1-4899-5031-4_5
Surface Receptors of Mononuclear Phagocytes
P. Mckeever (1980)
10.4044/JOMA1947.95.3-4_253
Studies on peripheral monocytes in diabetes mellitus
Kiyoshi Miyahara (1983)
10.1007/978-3-642-71234-0_24
Behavioral Influences on Neuroendocrines and Insulin Sensitivity as Precursors of Coronary Heart Disease
J. A. Herd (1986)
10.2337/diab.34.2.166
Effect of Insulin Antibodies on Insulin Pharmacokinetics and Glucose Utilization in Insulin-dependent Diabetic Patients
W. Waldhäusl (1985)
Stéatohépatite et adaptations métaboliques: Effets d'un régime enrichi en lipides saturés, ou carencé en choline et méthionine, sur la bioénergétique et le métabolisme hépatique de rat
C. Romestaing (2007)
10.1016/B978-0-12-039228-5.50010-3
Diabetes mellitus: relationships of nonhuman primates and other animal models to human forms of diabetes.
C. F. Howard (1984)
See more
Semantic Scholar Logo Some data provided by SemanticScholar