Online citations, reference lists, and bibliographies.
← Back to Search

Effect Of Feeding And Obesity On Lipoprotein Lipase Activity, Immunoreactive Protein, And Messenger RNA Levels In Human Adipose Tissue.

J. Ong, P. Kern
Published 1989 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Previous studies have demonstrated higher levels of adipose tissue lipoprotein lipase (LPL) catalytic activity in obese subjects, and in response to a meal. To examine the cellular mechanism of this increase in activity, LPL activity, immunoreactive mass, and mRNA level were measured in lean and obese subjects both before and 4 h after a carbohydrate-rich meal. Heparin-releasable (HR) LPL activity was approximately 2.5-fold higher in the 15 obese subjects, when compared with six lean subjects. However, there was no difference in LPL immunoreactive mass between the lean and obese subjects. In response to the meal, there was a 2.2-fold increase in total adipose tissue LPL activity in the lean subjects due to an increase in both the HR fraction, as well as the adipose fraction extracted with detergents. However, no increase in LPL immunoreactive mass was observed in any adipose tissue LPL fraction, resulting in an increase in LPL specific activity in response to the meal. In the obese subjects, there was no significant increase in LPL activity in response to feeding, and also no increase in immunoreactive mass or specific activity. After extraction of RNA, there was no difference in either the relative proportion of the 3.6- and 3.4-kb human LPL mRNA transcripts, nor in the quantity of LPL mRNA in response to feeding. Thus, these data suggest that the increase in LPL activity under these conditions occurs through a posttranslational activation of a previously inactive LPL precursor.
This paper references
10.1128/MCB.3.5.787
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
P. Gunning (1983)
10.1016/0005-2760(83)90060-7
Influence of nutritional state on lipoprotein lipase activities in the hypothyroid rat.
P. Hansson (1983)
10.1016/0026-0495(80)90063-3
Postprandial adipose tissue lipoprotein lipase activity in primary hypertriglyceridemia.
A. Goldberg (1980)
10.1172/JCI111675
Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes.
P. Kern (1985)
10.1172/JCI108185
Determinants of human adipose tissue lipoprotein lipase. Effect of diabetes and obesity on basal- and diet-induced activity.
O. Pykälistö (1975)
10.1172/JCI113332
Regulation of lipoprotein lipase immunoreactive mass in isolated human adipocytes.
P. Kern (1988)
10.1016/0003-2697(87)90021-2
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
10.1210/ENDO-114-5-1665
Insulin regulation of lipoprotein lipase in cultured isolated rat adipocytes.
R. Eckel (1984)
Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides.
P. Belfrage (1969)
10.1042/BJ1880193
Effects of glucose and insulin on the activation of lipoprotin lipase and on protein-synthesis in rat adipose tissue.
S. M. Parkin (1980)
10.1152/AJPLEGACY.1971.221.3.850
A simple method to determine fat cell size and number in four mammalian species.
M. di Girolamo (1971)
Lipoprotein lipase activity in adipose tissue from obese human beings.
W. Dahms (1981)
10.2337/diab.35.9.990
Effects of Weight Loss on Mechanisms of Hyperglycemia in Obese Non-Insulin-Dependent Diabetes Mellitus
R. Henry (1986)
Isolation and Characterization of Full-Length cDNA Clones for Human a-, 13-, and y-Actin mRNAs : Skeletal but Not Cytoplasmic Actins Have an Amino-Terminal Cysteine that Is Subsequently Removed
P. Gunning
10.1172/JCI110171
Increase of adipose tissue lipoprotein lipase activity with weight loss.
R. Schwartz (1981)
10.1126/SCIENCE.3823907
Human lipoprotein lipase complementary DNA sequence.
K. Wion (1987)
10.1111/J.0954-6820.1970.TB08030.X
Effects of prolonged fast on lipoprotein lipase activity eluted from human adipose tissue.
B. Persson (1970)
10.1016/0005-2760(76)90269-1
Intra- and extracellular forms of lipoprotein lipase in adipose tissue.
P. Nilsson-ehle (1976)
10.1007/BF01236268
Lipoprotein lipase activity of adipose tissue and skeletal muscle in insulin-deficient human diabetes
M. R. Taskinen (2005)
10.1016/0005-2760(86)90184-0
Maturation and secretion of lipoprotein lipase in cultured adipose cells. II. Effects of tunicamycin on activation and secretion of the enzyme.
E. Amri (1986)
The relationship of enzyme activity to feeding behavior in rats: lipoprotein lipase as the metabolic gatekeeper.
Greenwood Mr (1985)
10.1080/00365517509095754
Rapid Effects on Lipoprotein Lipase Activity in Adipose Tissue of Humans after Carbohydrate and Lipid Intake Time Course and Relation to Plasma Glycerol, Triglyceride, and Insulin Levels
P. Nilsson-ehle (1975)
10.1016/0005-2760(85)90235-8
Importance of the different steps of glycosylation for the activity and secretion of lipoprotein lipase in rat preadipocytes studied with monensin and tunicamycin.
T. Chajek-Shaul (1985)
10.1093/AJCN/37.3.387
Refeeding after fasting in the rat: effects of dietary-induced obesity on energy balance regulation.
D. Walks (1983)
Maturation and secretion of lipoprotein lipase in cultured adipose cells. I. Intracellular activation of the enzyme.
C. Vannier (1985)
10.1042/BJ2070485
Purification and characterization of rat adipose tissue lipoprotein lipase.
S. M. Parkin (1982)
The role of glucose and glycosylation in the regulation of lipoprotein lipase synthesis and secretion in rat adipocytes.
J. Ong (1989)
10.1042/BJ1760865
Post-translational regulation of lipoprotein lipase activity in adipose tissue.
P. Ashby (1978)
10.1016/0003-2697(83)90418-9
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Andrew P. Feinberg (1983)
10.1042/cs044023pa
Lipoprotein lipase in human adipose tissue.
R. Elkeles (1973)
10.1016/0005-2760(78)90157-1
The effect of nutritional state on the lipoprotein lipase activity of isolated fat cells.
I. M. Spencer (1978)
10.1016/0076-6879(86)29099-0
Preparation, characterization, and measurement of lipoprotein lipase.
P. Iverius (1986)
Insulin increases the synthetic rate and messenger RNA level of lipoprotein lipase in isolated rat adipocytes.
J. Ong (1988)
10.1172/JCI110547
Insulin stimulation of adipose tissue lipoprotein lipase. Use of the euglycemic clamp technique.
C. Sadur (1982)
Insulin regulation of adipose tissue lipoprotein lipase in cultured rat adipocytes
P. H. Smith (1984)
Insulin and adipose tissue lipoprotein lipase activity in humans.
J. Brunzell (1981)
10.1016/0026-0495(84)90235-X
Fat feeding decreases insulin responsiveness of adipose tissue lipoprotein lipase.
C. Sadur (1984)
10.1016/0005-2760(78)90187-X
Regulation of lipoprotein lipase immunological study of adipose tissue.
H. Jansen (1978)



This paper is referenced by
10.1210/JCEM.83.8.4998
Serum lipids, lipoproteins, and lipid metabolizing enzymes in identical twins discordant for obesity.
T. Rönnemaa (1998)
10.1016/0005-2760(93)90196-G
Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level.
S. Enerbäck (1993)
10.1155/2012/603501
Hypolipidemic Activity of Eryngium carlinae on Streptozotocin-Induced Diabetic Rats
Ruth Noriega-Cisneros (2012)
10.1152/AJPENDO.00398.2003
Differential regulation of fatty acid trapping in mouse adipose tissue and muscle by ASP.
M. Faraj (2004)
10.1074/JBC.270.13.7149
Tissue-specific Expression of Human Lipoprotein Lipase
G. Ranganathan (1995)
10.1210/jc.2008-2090
Fasted to fed trafficking of Fatty acids in human adipose tissue reveals a novel regulatory step for enhanced fat storage.
T. Ruge (2009)
10.1017/S0029665199001184
Macronutrient metabolism of adipose tissue at rest and during exercise: a methodological viewpoint.
K. Frayn (1999)
10.1152/AJPENDO.1992.263.3.E500
Regulation of expression of the lipoprotein lipase gene in brown adipose tissue.
J. R. Mitchell (1992)
10.1201/B16473-21
Insulin Resistance and Obesity
Charmaine S Tam (2014)
10.1210/JC.2004-2343
In vivo evidence of impaired peripheral fatty acid trapping in patients with human immunodeficiency virus-associated lipodystrophy.
J. V. van Wijk (2005)
10.1385/1-59259-231-7:269
Assessment of white adipose tissue metabolism by measurement of arteriovenous differences.
K. Frayn (2001)
10.1016/j.tem.2020.02.006
How May GIP Enhance the Therapeutic Efficacy of GLP-1?
Ricardo J. Samms (2020)
Expression of lipoprotein lipase in rat muscle: regulation by feeding and hypothyroidism.
J. Ong (1994)
10.1007/978-1-4899-2575-6_4
Cellular mechanisms for the regulation of adipose tissue lipid metabolism in pregnancy and lactation.
D. Williamson (1994)
Effects of cytokines on the production of lipoprotein lipase in cultured human macrophages.
U. Querfeld (1990)
Relation between Abdominal Fat and Serum Cholesterol, Triglycerides, and Lipoprotein Concentrations in Chicken Breeds
Hassan Musa (2007)
10.1016/0026-0495(94)90244-5
Reduced adipose tissue lipoprotein lipase responses, postprandial lipemia, and low high-density lipoprotein-2 subspecies levels in older athletes with silent myocardial ischemia.
L. Katzel (1994)
10.3181/0904-RM-140
Response to Fasting in an Unnaturally Obese Carnivore, the Captive European Polecat Mustela putorius
Anne-Mari Mustonen (2009)
10.1016/j.metabol.2012.12.002
Increased nocturnal fat oxidation in young healthy men with low birth weight: results from 24-h whole-body respiratory chamber measurements.
Charlotte Brøns (2013)
10.1194/jlr.M600248-JLR200
The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment Published, JLR Papers in Press, August 7, 2006.
G. Ranganathan (2006)
10.1210/ENDO.130.4.1547742
The regulation of lipoprotein lipase gene expression by dexamethasone in isolated rat adipocytes.
J. Ong (1992)
10.1210/MEND.6.1.1738372
Epinephrine inhibits lipoprotein lipase gene expression in rat adipocytes through multiple steps in posttranscriptional processing.
J. Ong (1992)
10.1111/obr.12298
Targeting fatty acid metabolism to improve glucose metabolism
R. Stinkens (2015)
10.1007/s004390000292
Lipoprotein lipase gene variation is associated with adipose tissue lipoprotein lipase activity, and lipoprotein lipid and glucose concentrations in overweight postmenopausal women
B. Nicklas (2000)
10.1007/s00125-002-0873-y
Adipose tissue as a buffer for daily lipid flux
K. Frayn (2002)
10.1079/PNS200195
Adipose tissue and the insulin resistance syndrome.
K. Frayn (2001)
10.1016/S0076-6879(96)63007-9
Apolipoprotein B-48.
F. Karpe (1996)
Mecanismos de regulación de la lipoproteinalipasa. Relación con los desórdenes metabólicos de la obesidad
Laura Martín Pozo (2014)
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.1152/AJPENDO.00307.2001
Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet.
F. Picard (2002)
10.1152/AJPENDO.1992.262.5.E695
Effects of insulin and dexamethasone on lipoprotein lipase in human adipose tissue.
B. Appel (1992)
Factors determining the progression of nonalcoholic fatty liver disease : the role of abnormal fatty acid and glucocorticoid metabolism
D. Macfarlane (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar