← Back to Search
Effect Of Feeding And Obesity On Lipoprotein Lipase Activity, Immunoreactive Protein, And Messenger RNA Levels In Human Adipose Tissue.
J. Ong, P. Kern
Published 1989 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Previous studies have demonstrated higher levels of adipose tissue lipoprotein lipase (LPL) catalytic activity in obese subjects, and in response to a meal. To examine the cellular mechanism of this increase in activity, LPL activity, immunoreactive mass, and mRNA level were measured in lean and obese subjects both before and 4 h after a carbohydrate-rich meal. Heparin-releasable (HR) LPL activity was approximately 2.5-fold higher in the 15 obese subjects, when compared with six lean subjects. However, there was no difference in LPL immunoreactive mass between the lean and obese subjects. In response to the meal, there was a 2.2-fold increase in total adipose tissue LPL activity in the lean subjects due to an increase in both the HR fraction, as well as the adipose fraction extracted with detergents. However, no increase in LPL immunoreactive mass was observed in any adipose tissue LPL fraction, resulting in an increase in LPL specific activity in response to the meal. In the obese subjects, there was no significant increase in LPL activity in response to feeding, and also no increase in immunoreactive mass or specific activity. After extraction of RNA, there was no difference in either the relative proportion of the 3.6- and 3.4-kb human LPL mRNA transcripts, nor in the quantity of LPL mRNA in response to feeding. Thus, these data suggest that the increase in LPL activity under these conditions occurs through a posttranslational activation of a previously inactive LPL precursor.
This paper references
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
P. Gunning (1983)
Influence of nutritional state on lipoprotein lipase activities in the hypothyroid rat.
P. Hansson (1983)
Postprandial adipose tissue lipoprotein lipase activity in primary hypertriglyceridemia.
A. Goldberg (1980)
Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes.
P. Kern (1985)
Determinants of human adipose tissue lipoprotein lipase. Effect of diabetes and obesity on basal- and diet-induced activity.
O. Pykälistö (1975)
Regulation of lipoprotein lipase immunoreactive mass in isolated human adipocytes.
P. Kern (1988)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
Insulin regulation of lipoprotein lipase in cultured isolated rat adipocytes.
R. Eckel (1984)
Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides.
P. Belfrage (1969)
Effects of glucose and insulin on the activation of lipoprotin lipase and on protein-synthesis in rat adipose tissue.
S. M. Parkin (1980)
A simple method to determine fat cell size and number in four mammalian species.
M. di Girolamo (1971)
Lipoprotein lipase activity in adipose tissue from obese human beings.
W. Dahms (1981)
Effects of Weight Loss on Mechanisms of Hyperglycemia in Obese Non-Insulin-Dependent Diabetes Mellitus
R. Henry (1986)
Isolation and Characterization of Full-Length cDNA Clones for Human a-, 13-, and y-Actin mRNAs : Skeletal but Not Cytoplasmic Actins Have an Amino-Terminal Cysteine that Is Subsequently Removed
Increase of adipose tissue lipoprotein lipase activity with weight loss.
R. Schwartz (1981)
Human lipoprotein lipase complementary DNA sequence.
K. Wion (1987)
Effects of prolonged fast on lipoprotein lipase activity eluted from human adipose tissue.
B. Persson (1970)
Intra- and extracellular forms of lipoprotein lipase in adipose tissue.
P. Nilsson-ehle (1976)
Lipoprotein lipase activity of adipose tissue and skeletal muscle in insulin-deficient human diabetes
M. R. Taskinen (2005)
Maturation and secretion of lipoprotein lipase in cultured adipose cells. II. Effects of tunicamycin on activation and secretion of the enzyme.
E. Amri (1986)
The relationship of enzyme activity to feeding behavior in rats: lipoprotein lipase as the metabolic gatekeeper.
Greenwood Mr (1985)
Rapid Effects on Lipoprotein Lipase Activity in Adipose Tissue of Humans after Carbohydrate and Lipid Intake Time Course and Relation to Plasma Glycerol, Triglyceride, and Insulin Levels
P. Nilsson-ehle (1975)
Importance of the different steps of glycosylation for the activity and secretion of lipoprotein lipase in rat preadipocytes studied with monensin and tunicamycin.
T. Chajek-Shaul (1985)
Refeeding after fasting in the rat: effects of dietary-induced obesity on energy balance regulation.
D. Walks (1983)
Maturation and secretion of lipoprotein lipase in cultured adipose cells. I. Intracellular activation of the enzyme.
C. Vannier (1985)
Purification and characterization of rat adipose tissue lipoprotein lipase.
S. M. Parkin (1982)
The role of glucose and glycosylation in the regulation of lipoprotein lipase synthesis and secretion in rat adipocytes.
J. Ong (1989)
Post-translational regulation of lipoprotein lipase activity in adipose tissue.
P. Ashby (1978)
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Andrew P. Feinberg (1983)
Lipoprotein lipase in human adipose tissue.
R. Elkeles (1973)
The effect of nutritional state on the lipoprotein lipase activity of isolated fat cells.
I. M. Spencer (1978)
Preparation, characterization, and measurement of lipoprotein lipase.
P. Iverius (1986)
Insulin increases the synthetic rate and messenger RNA level of lipoprotein lipase in isolated rat adipocytes.
J. Ong (1988)
Insulin stimulation of adipose tissue lipoprotein lipase. Use of the euglycemic clamp technique.
C. Sadur (1982)
Insulin regulation of adipose tissue lipoprotein lipase in cultured rat adipocytes
P. H. Smith (1984)
Insulin and adipose tissue lipoprotein lipase activity in humans.
J. Brunzell (1981)
Fat feeding decreases insulin responsiveness of adipose tissue lipoprotein lipase.
C. Sadur (1984)
Regulation of lipoprotein lipase immunological study of adipose tissue.
H. Jansen (1978)
This paper is referenced by
Serum lipids, lipoproteins, and lipid metabolizing enzymes in identical twins discordant for obesity.
T. Rönnemaa (1998)
Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level.
S. Enerbäck (1993)
Hypolipidemic Activity of Eryngium carlinae on Streptozotocin-Induced Diabetic Rats
Ruth Noriega-Cisneros (2012)
Differential regulation of fatty acid trapping in mouse adipose tissue and muscle by ASP.
M. Faraj (2004)
Tissue-specific Expression of Human Lipoprotein Lipase
G. Ranganathan (1995)
Fasted to fed trafficking of Fatty acids in human adipose tissue reveals a novel regulatory step for enhanced fat storage.
T. Ruge (2009)
Macronutrient metabolism of adipose tissue at rest and during exercise: a methodological viewpoint.
K. Frayn (1999)
Regulation of expression of the lipoprotein lipase gene in brown adipose tissue.
J. R. Mitchell (1992)
Insulin Resistance and Obesity
Charmaine S Tam (2014)
In vivo evidence of impaired peripheral fatty acid trapping in patients with human immunodeficiency virus-associated lipodystrophy.
J. V. van Wijk (2005)
Assessment of white adipose tissue metabolism by measurement of arteriovenous differences.
K. Frayn (2001)
How May GIP Enhance the Therapeutic Efficacy of GLP-1?
Ricardo J. Samms (2020)
Expression of lipoprotein lipase in rat muscle: regulation by feeding and hypothyroidism.
J. Ong (1994)
Cellular mechanisms for the regulation of adipose tissue lipid metabolism in pregnancy and lactation.
D. Williamson (1994)
Effects of cytokines on the production of lipoprotein lipase in cultured human macrophages.
U. Querfeld (1990)
Relation between Abdominal Fat and Serum Cholesterol, Triglycerides, and Lipoprotein Concentrations in Chicken Breeds
Hassan Musa (2007)
Reduced adipose tissue lipoprotein lipase responses, postprandial lipemia, and low high-density lipoprotein-2 subspecies levels in older athletes with silent myocardial ischemia.
L. Katzel (1994)
Response to Fasting in an Unnaturally Obese Carnivore, the Captive European Polecat Mustela putorius
Anne-Mari Mustonen (2009)
Increased nocturnal fat oxidation in young healthy men with low birth weight: results from 24-h whole-body respiratory chamber measurements.
Charlotte Brøns (2013)
The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment Published, JLR Papers in Press, August 7, 2006.
G. Ranganathan (2006)
The regulation of lipoprotein lipase gene expression by dexamethasone in isolated rat adipocytes.
J. Ong (1992)
Epinephrine inhibits lipoprotein lipase gene expression in rat adipocytes through multiple steps in posttranscriptional processing.
J. Ong (1992)
Targeting fatty acid metabolism to improve glucose metabolism
R. Stinkens (2015)
Lipoprotein lipase gene variation is associated with adipose tissue lipoprotein lipase activity, and lipoprotein lipid and glucose concentrations in overweight postmenopausal women
B. Nicklas (2000)
Adipose tissue as a buffer for daily lipid flux
K. Frayn (2002)
Adipose tissue and the insulin resistance syndrome.
K. Frayn (2001)
F. Karpe (1996)
Mecanismos de regulación de la lipoproteinalipasa. Relación con los desórdenes metabólicos de la obesidad
Laura Martín Pozo (2014)
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet.
F. Picard (2002)
Effects of insulin and dexamethasone on lipoprotein lipase in human adipose tissue.
B. Appel (1992)
Factors determining the progression of nonalcoholic fatty liver disease : the role of abnormal fatty acid and glucocorticoid metabolism
D. Macfarlane (2011)See more