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Tumor Necrosis Factor-alpha Eliminates Binding Of NF-Y And An Octamer-binding Protein To The Lipoprotein Lipase Promoter In 3T3-L1 Adipocytes.

C. Morin, I. Schlaepfer, R. Eckel
Published 1995 · Biology, Medicine

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TNF alpha has been shown to reduce lipoprotein lipase (LPL) activity in adipose tissue. Regulation of LPL by TNF alpha occurs at the level of LPL gene transcription and posttranscriptionally. To elucidate further the transcriptional mechanism of TNF alpha inhibition of LPL gene transcription, transfection analysis was used to locate the site(s) of the LPL promoter that imparts the TNF alpha response. Transient transfections using LPL promoter deletions fused to luciferase in differentiated 3T3-L1 cells with and without TNF alpha treatment indicated that a DNA region downstream of -180 bp confers the TNF alpha effect. Electrophoretic mobility shift assays using two 32P-labeled LPL probes spanning the region between -180 and +44 bp revealed the loss of several LPL DNA-protein interactions after TNF alpha treatment, including the binding of NF-Y to the CCAAT box and a protein to the octamer consensus sequence. Protein binding to the OCT-1 consensus sequence is unaffected until after 4 h of TNF alpha treatment. In addition, the amount of mRNA for OCT-1 is not altered with TNF alpha treatment. These results indicate that TNF alpha regulates at least two DNA-binding proteins on the proximal promoter, thereby inhibiting LPL gene transcription.

This paper is referenced by
Lipoprotein lipase: genetics, lipid uptake, and regulation.
M. Merkel (2002)
Unraveling the role of nicotinamide phosphoribosyltransferase on lipids in atherosclerosis
Tuva B. Dahl (2012)
Exploring Therapeutic Targets to Reverse or Prevent the Transition from Metabolically Healthy to Unhealthy Obesity
Tenzin D Dagpo (2020)
NF-Y and the transcriptional activation of CCAAT promoters
D. Dolfini (2012)
Sepsis and fat metabolism
J. Samra (1996)
Obesity and Diabetes in TNF-a Receptor – deficient Mice
S. Schreyer (1998)
Nonexercise activity thermogenesis (NEAT): environment and biology.
J. Levine (2004)
Transcription factors CCAAT/enhancer-binding protein beta and nuclear factor-Y bind to discrete regulatory elements in the very low density lipoprotein receptor promoter.
R. Kreuter (1999)
Tumor necrosis factor-alpha stimulates cell proliferation in adipose tissue-derived stromal-vascular cell culture: promotion of adipose tissue expansion by paracrine growth factors.
K. Kras (2000)
Endothelin-stimulated Ca2+ mobilization by 3T3-L1 adipocytes is suppressed by tumor necrosis factor-alpha.
M. Yorek (1999)
Polymorphism of stress protein HSP70-2 gene in Tunisians: susceptibility implications in type 2 diabetes and obesity.
K. Zouari Bouassida (2004)
Epigenetic regulation of lipoprotein lipase gene via BRD4, which is potentially associated with adipocyte differentiation and insulin resistance.
T. Inoue (2019)
Exercise and Health-Related Risks of Physical Deconditioning After Spinal Cord Injury.
Jennifer L. Maher (2017)
Susceptibilidad genética a la cirrosis
I. Rojas (2005)
Lipoprotein lipase: the regulation of tissue specific expression and its role in lipid and energy metabolism
Karina Preiss-Landl (2002)
Adipose Tissue-Derived Tumor Necrosis Factor-α Activity Is Elevated in Older Rats
C. Morin (1997)
Prevention of diet-induced obesity in transgenic mice overexpressing skeletal muscle lipoprotein lipase.
D. R. Jensen (1997)
The molecular biology of the CCAAT-binding factor NF-Y.
Roberto Mantovani (1999)
Obesity and diabetes in TNF-alpha receptor- deficient mice.
S. Schreyer (1998)
SHORT COMMUNICATION Tumor necrosis factor-a7308 G=A polymorphism in obese Caucasians
E. Brand (2001)
Tumor necrosis factor-α−308 G/A polymorphism in obese Caucasians
E. Brand (2001)
Characterisation of the tumour necrosis factor (TNF)-(alpha) response elements in the human ICAM-2 promoter.
F. Mclaughlin (1999)
Quantifying Serum TNF-Alpha Cut-Off Point for Predicting Coronary Stenosis Severity in a Population from Northern Iran
Bijan Shad (2014)
Lipoprotein lipase: from gene to obesity.
H. Wang (2009)
Lipoprotein Lipase as a Candidate Target for Cancer Prevention/Therapy
S. Takasu (2012)
Sp1 and Sp3 transactivate the human lipoprotein lipase gene promoter through binding to a CT element: synergy with the sterol regulatory element binding protein and reduced transactivation of a naturally occurring promoter variant.
W. Yang (1998)
Role of immune mediators in metabolic syndrome and atherosclerosis
D. Strodthoff (2013)
Lipoprotein lipase: from gene to atherosclerosis.
Y. Li (2014)
Tumor necrosis factor alpha is a potent regulator in fish adipose tissue
D. Liu (2015)
Molecular cloning of adipose triglyceride lipase (ATGL) gene from blunt snout bream and its expression after LPS-induced TNF-α factor
Yong-jun Dai (2018)
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages Published, JLR Papers in Press, July 20, 2007.
Guosong Qiu (2007)
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