Online citations, reference lists, and bibliographies.
← Back to Search

DNA Aptamers Block L-selectin Function In Vivo. Inhibition Of Human Lymphocyte Trafficking In SCID Mice.

B. Hicke, S. Watson, A. König, C. K. Lynott, R. Bargatze, Y. Chang, S. Ringquist, L. Moon-McDermott, S. Jennings, T. Fitzwater, H. Han, N. Varki, I. Albinana, M. C. Willis, A. Varki, D. Parma
Published 1996 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Selectins participate in the initial events leading to leukocyte extravasation from the blood into tissues. Thus the selectins have generated much interest as targets for antiinflammatory agents. Therapeutic molecules based on the monomeric carbohydrate ligand sialyl Lewis X (SLe(X)) have low affinities and are not specific for a given selectin. Using SELEX (Systematic Evolution of Ligands by EXponential Enrichment) technology, we have generated aptamers specific for L-selectin that require divalent cations for binding and have low nanomolar affinity. In vitro, the deoxyoligonucleotides inhibit L-selectin binding to immobilized SLe(X) in static assays and inhibit L-selectin-mediated rolling of human lymphocytes and neutrophils on cytokine-activated endothelial cells in flow-based assays. These aptamers also block L-selectin-dependent lymphocyte trafficking in vivo, indicating their potential utility as therapeutics.
This paper references
Selectins. J. Clin. Invest
M P Bevilacqua (1993)
10.1083/JCB.120.5.1227
P- and E-selectin use common sites for carbohydrate ligand recognition and cell adhesion
D. Erbe (1993)
10.1074/JBC.270.23.13581
Oligonucleotides as Research, Diagnostic, and Therapeutic Agents(*)
L. Gold (1995)
CD22, a B cell-specific immunoglobulin superfamily member, is a sialic acid-binding lectin.
D. Sgroi (1993)
10.1073/PNAS.93.12.5883
Calcium-dependent oligonucleotide antagonists specific for L-selectin.
D. O'connell (1996)
Long-term CD4+ memory T cells from the spleen lack MEL-14, the lymph node homing receptor.
L. Bradley (1992)
10.1083/JCB.119.1.215
Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion [published erratum appears in J Cell Biol 1993 Feb;120(4):1071]
D. Erbe (1992)
10.1126/SCIENCE.2200121
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
C. Tuerk (1990)
10.1146/ANNUREV.MED.45.1.361
Endothelial-leukocyte adhesion molecules in human disease.
M. Bevilacqua (1994)
Characterization of a specific ligand for P-selectin on myeloid cells. A minor glycoprotein with sialylated O-linked oligosaccharides.
K. Norgard (1993)
10.1096/fasebj.8.8.8181668
Adhesion molecules and inflammatory injury
S. Albelda (1994)
10.1126/SCIENCE.7692600
Binding of L-selectin to the vascular sialomucin CD34.
S. Baumheter (1993)
10.1038/304030a0
A cell-surface molecule involved in organ-specific homing of lymphocytes
W. Gallatin (1983)
10.1016/0092-8674(92)90612-G
An endothelial ligand for L-Selectin is a novel mucin-like molecule
L. Lasky (1992)
10.1146/ANNUREV.BI.64.070195.000553
Selectin-carbohydrate interactions and the initiation of the inflammatory response.
L. Lasky (1995)
10.1073/PNAS.87.6.2244
Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
T. Kishimoto (1990)
10.1016/0092-8674(93)90327-M
Expression cloning of a functional glycoprotein ligand for P-selectin
D. Sako (1993)
10.1016/0092-8674(94)90337-9
Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigm
T. Springer (1994)
10.1146/ANNUREV.IY.10.040192.003021
Physiological and molecular mechanisms of lymphocyte homing.
L. Picker (1992)
10.1016/0955-0674(94)90092-2
The selectins and their ligands.
S. D. Rosen (1994)
10.1146/ANNUREV.BI.64.070195.003555
Diversity of oligonucleotide functions.
L. Gold (1995)
10.1074/JBC.270.19.11025
Leukocyte trafficking mediated by selectin-carbohydrate interactions
R. McEver (1995)
10.1083/JCB.117.4.895
The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide
C. Foxall (1992)
A carbohydrate domain common to both sialyl Le(a) and sialyl Le(X) is recognized by the endothelial cell leukocyte adhesion molecule ELAM-1.
E. L. Berg (1991)
10.1182/BLOOD.V84.7.2068.BLOODJOURNAL8472068
Leukocyte-endothelial adhesion molecules.
T. Carlos (1994)
10.1073/PNAS.91.16.7390
Selectin ligands.
A. Varki (1994)
In vivo and in vitro functional examination of a conserved epitope of L- and E-selectin crucial for leukocyte-endothelial cell interactions.
R. Bargatze (1994)
10.1172/JCI116275
Higher-affinity oligosaccharide ligands for E-selectin.
R. M. Nelson (1993)
Neutrophil recognition requires a Ca(2+)-induced conformational change in the lectin domain of GMP-140.
J. G. Geng (1991)
10.1093/CVR/28.3.289
Role of selectins, a new family of adhesion molecules, in ischaemia-reperfusion injury.
A. Lefer (1994)



This paper is referenced by
10.1007/BF03044858
Aptamere: ein neuer Ansatz für Interventionsstudien und zur Entwicklung zukünftiger Therapieansätze
T. Ostendorf (2009)
10.1016/j.ijpharm.2019.118483
Aptamers as the chaperones of drugs-from siRNAs to DNA nanorobots.
Marimuthu Citartan (2019)
10.1038/nprot.2010.66
Development of DNA aptamers using Cell-SELEX
K. Sefah (2010)
10.1023/A:1026696330245
Combinatorial Chemistry of Nucleic Acids: SELEX
A. Kopylov (2004)
Ligands d'acide nucleique a haute affinite pour les lectines
D. Parma (1996)
10.1023/A:1016110724564
DNA Aptamers as Radically New Recognition Elements for Biosensors
V. Spiridonova (2004)
10.1246/BCSJ.81.1485
3' Poly(dA)-Tailed Thrombin DNA Aptamer to Increase DNase-Resistance and Clotting Inhibitory Activity
S. Uehara (2008)
10.1172/JCI1509
Differential interactions of heparin and heparan sulfate glycosaminoglycans with the selectins. Implications for the use of unfractionated and low molecular weight heparins as therapeutic agents.
A. König (1998)
10.1016/S0196-9781(02)00024-4
Role of SA–Lea and E-selectin in metastasis assessed with peptide antagonist
O. Insug (2002)
10.1159/000145542
Single-Stranded DNA Oligoaptamers: Molecular Recognition and LPS Antagonism Are Length- and Secondary Structure-Dependent
J. Ding (2008)
10.4161/pri.1.4.5803
Screening of DNA Aptamer Against Mouse Prion Protein by Competitive Selection
Daisuke Ogasawara (2007)
10.1002/bit.25294
A simple method for eliminating fixed‐region interference of aptamer binding during SELEX
E. Ouellet (2014)
10.1002/smll.201002292
Targeted cell-cell interactions by DNA nanoscaffold-templated multivalent bispecific aptamers.
Xiaowei Liu (2011)
Therapeutic inhibition of the complement system.
S. C. Makrides (1998)
10.1089/hum.2008.045
Aptamers in immunotherapy.
Claudia M Dollins (2008)
10.1517/14728222.9.2.225
Lymphocyte trafficking to inflamed skin – molecular mechanisms and implications for therapeutic target molecules
M. Schön (2005)
10.1038/cr.2015.7
Characterization and target identification of a DNA aptamer that labels pluripotent stem cells
Zhonggang Hou (2015)
10.1016/j.bios.2011.02.041
Thermo-responsive molecular switches for ATP using hairpin DNA aptamers.
T. Goda (2011)
10.31274/ETD-180810-93
Function and dynamics of aptamers: A case study on the malachite green aptamer
T. Wang (2008)
10.1073/pnas.0831159100
Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2
R. White (2003)
10.1134/S0026893309040153
Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein
E. R. Surina (2009)
10.1038/labinvest.3700417
Anti-Francisella tularensis DNA aptamers detect tularemia antigen from different subspecies by Aptamer-Linked Immobilized Sorbent Assay
J. Vivekananda (2006)
10.1021/ja103023m
Enhanced Functional Potential of Nucleic Acid Aptamer Libraries Patterned to Increase Secondary Structure
Karen M Ruff (2010)
10.1016/S0022-510X(98)00154-3
Role of the leukocyte-adhesion molecule L-selectin in experimental autoimmune encephalomyelitis
J. Archelos (1998)
Directed molecular evolution of novel DNA aptamers raised against an antibiotic resistant Escherichia coli
Robert Michael Nicholas Gowland (2015)
10.1517/17460441.2.9.1205
Characterisation of aptamers for therapeutic studies
Aniela Wochner (2007)
10.4061/2011/904750
Cell-Specific Aptamers as Emerging Therapeutics
C. Meyer (2011)
10.1007/978-1-4419-0919-0_17
Aptamers: Versatile Tools for Reagentless Aptasensing
E. Baldrich (2010)
10.3390/s17020226
Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography
C. Kuehne (2017)
10.1201/B12935-4
Aptamer-Functionalized Nanomaterials for Cell Recognition
Jing Cheng Zhou (2012)
Functional DNA Aptamers as Biotherapeutic Molecules
Erik W Orava (2014)
10.1007/978-94-007-5869-8_22
Aptamers as New Agents Against Biotoxins
Shuowei Cai (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar