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Characterization And Genomic Analysis Of Chromate Resistant And Reducing Bacillus Cereus Strain SJ1

Minyan He, X. Li, L. Guo, S. J. Miller, C. Rensing, G. Wang
Published 2010 · Biology, Medicine

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BackgroundChromium is a toxic heavy metal, which primarily exists in two inorganic forms, Cr(VI) and Cr(III). Chromate [Cr(VI)] is carcinogenic, mutational, and teratogenic due to its strong oxidizing nature. Biotransformation of Cr(VI) to less-toxic Cr(III) by chromate-resistant and reducing bacteria has offered an ecological and economical option for chromate detoxification and bioremediation. However, knowledge of the genetic determinants for chromate resistance and reduction has been limited so far. Our main aim was to investigate chromate resistance and reduction by Bacillus cereus SJ1, and to further study the underlying mechanisms at the molecular level using the obtained genome sequence.ResultsBacillus cereus SJ1 isolated from chromium-contaminated wastewater of a metal electroplating factory displayed high Cr(VI) resistance with a minimal inhibitory concentration (MIC) of 30 mM when induced with Cr(VI). A complete bacterial reduction of 1 mM Cr(VI) was achieved within 57 h. By genome sequence analysis, a putative chromate transport operon, chrIA 1, and two additional chrA genes encoding putative chromate transporters that likely confer chromate resistance were identified. Furthermore, we also found an azoreductase gene azoR and four nitroreductase genes nitR possibly involved in chromate reduction. Using reverse transcription PCR (RT-PCR) technology, it was shown that expression of adjacent genes chrA 1 and chrI was induced in response to Cr(VI) but expression of the other two chromate transporter genes chrA 2 and chrA 3 was constitutive. In contrast, chromate reduction was constitutive in both phenotypic and gene expression analyses. The presence of a resolvase gene upstream of chrIA 1, an arsenic resistance operon and a gene encoding Tn7-like transposition proteins ABBCCCD downstream of chrIA 1 in B. cereus SJ1 implied the possibility of recent horizontal gene transfer.ConclusionOur results indicate that expression of the chromate transporter gene chrA 1 was inducible by Cr(VI) and most likely regulated by the putative transcriptional regulator ChrI. The bacterial Cr(VI)-resistant level was also inducible. The presence of an adjacent arsenic resistance gene cluster nearby the chrIA 1 suggested that strong selective pressure by chromium and arsenic could cause bacterial horizontal gene transfer. Such events may favor the survival and increase the resistance level of B. cereus SJ1.
This paper references
Multiple Metal Resistant Transferable Phenotypes in Bacteria as Indicators of Soil Contamination with Heavy Metals (6 pp)
R. Ryan (2005)
Reduction of Hexavalent Chromium by Cell-Free Extract of Bacillus sphaericus AND 303 Isolated from Serpentine Soil
Arundhati Pal (2005)
Reduction of chromate by cell-free extract of Brucella sp. isolated from Cr(VI) contaminated sites.
Urvashi Thacker (2007)
Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa.
A. H. Álvarez (1999)
A putative azoreductase gene is involved in the Shewanella oneidensis response to heavy metal stress
Irina Mugerfeld (2009)
Removal of toxic chromate using free and immobilized Cr(VI)-reducing bacterial cells of Intrasporangium sp. Q5-1
J. Yang (2009)
Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate
J. McLean (2001)
Hexavalent chromate reduction by immobilized Streptomyces griseus
Ashwini C. Poopal (2008)
Dosage-dependent proteome response of Shewanella oneidensis MR-1 to acute chromate challenge.
M. R. Thompson (2007)
Macaskie LE: Chromate reduction and 16 S rRNA identification of bacteria isolated from a Cr (VI)-contaminated site
P Pattanapipitpaisal (2001)
Hexavalent chromium reduction by Providencia sp
Urvashi Thacker (2006)
Chromatereducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli
DF Ackerley (2004)
A combined bacterial process for the reduction and immobilization of chromium
M. Viera (2003)
Hexavalent chromium reduction in Desulfovibrio vulgaris Hildenborough causes transitory inhibition of sulfate reduction and cell growth
A. Klonowska (2008)
Inducible metabolism of phenolic acids in Pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator.
L. Barthelmebs (2000)
Vibrio harveyi Nitroreductase Is Also a Chromate Reductase
Young Hak Kwak (2003)
A membrane-associated protein with Cr(VI)-reducing activity from Thermus scotoductus SA-01.
D. Opperman (2008)
New genes involved in chromate resistance in Ralstonia metallidurans strain CH34
S. Juhnke (2004)
Phylogenetic analysis of the chromate ion transporter (CHR) superfamily
C. Díaz-Pérez (2007)
Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake
Revati Wani (2007)
Bmc Microbiology
C. Parker (2003)
Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain
J. Campos (2004)
The chromate-inducible chrBACF operon from the transposable element TnOtChr confers resistance to chromium(VI) and superoxide.
R. Branco (2008)
Reduction and precipitation of chromate by mixed culture sulphate‐reducing bacterial biofilms
W. Smith (2000)
The RAST Server: Rapid Annotations using Subsystems Technology
R. Aziz (2007)
Chromate reduction and 16S rRNA identification of bacteria isolated from a Cr(VI)-contaminated site
P. Pattanapipitpaisal (2001)
Chromate-Reducing Properties of Soluble Flavoproteins from Pseudomonas putida and Escherichia coli
D. Ackerley (2004)
Efflux of chromate by Pseudomonas aeruginosa cells expressing the ChrA protein.
Betzabe E Pimentel (2002)
Bioremediation of chromate: thermodynamic analysis of the effects of Cr(VI) on sulfate-reducing bacteria
B. Chardin (2002)
Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
He (2010)
Amembrane - associated protein with Cr ( VI )reducing activity from Thermus scotoductus SA01
DJ Opperman (2007)
Hexavalent-chromium reduction by a chromate-resistant Bacillus sp
J Campos (1995)
Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics.
G. Puzon (2005)
Hexavalent Chromium Removal by a Trichoderma inhamatum Fungal Strain Isolated from Tannery Effluent
L. Morales-Barrera (2007)
Chromium(VI)-reducing Chlorella spp. isolated from disposal sites of paper-pulp and electroplating industry
S. Yewalkar (2007)
CHRD, a novel domain in the BMP inhibitor chordin, is also found in microbial proteins.
M. Hyvönen (2003)
Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction.
D. Ackerley (2004)
Aerobic Cr(VI) reduction by Thermus scotoductus strain SA‐01
D. Opperman (2007)
Cloning, Deletion, and Characterization of PadR, the Transcriptional Repressor of the Phenolic Acid Decarboxylase-Encoding padA Gene of Lactobacillus plantarum
J. Gury (2004)
Genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils
L. Cai (2008)
Expression of chromate resistance genes from Shewanella sp. strain ANA-3 in Escherichia coli.
Esther Aguilar-Barajas (2008)

This paper is referenced by
Chromium(VI) Biosorption and Bioaccumulation by Live and Acid-Modified Biomass of a Novel Morganella morganii Isolate
Zeynep Ergul-Ulger (2014)
Role of Bacterial Chromate Reductase in Bioremediation of Chromium-Containing Wastes
Satarupa Dey (2021)
Analysis of the Genome and Chromium Metabolism-Related Genes of Serratia sp. S2
Lanlan Dong (2017)
Reviews of Environmental Contamination and Toxicology Volume 217
David M. Whitacre (2012)
The mechanism of nitrate-Cr(VI) reduction mediated by microbial under different initial pHs.
Yutian Hu (2020)
In vitro Cr(VI) reduction by cell-free extracts of chromate-reducing bacteria isolated from tannery effluent irrigated soil
S. Soni (2012)
An approach to study ultrastructural changes and adaptive strategies displayed by Acinetobacter guillouiae SFC 500-1A under simultaneous Cr(VI) and phenol treatment
Marilina Fernandez (2017)
Simultaneous removal of chromate and arsenite by the immobilized Enterobacter bacterium in combination with chemical reagents
Kaixiang Shi (2020)
Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge
Paul Fabrice Nguema (2017)
Exposure of Soil Microbial Communities to Chromium and Arsenic Alters Their Diversity and Structure
C. Sheik (2012)
Identification and Characterization of the Transcriptional Regulator ChrB in the Chromate Resistance Determinant of Ochrobactrum tritici 5bvl1
R. Branco (2013)
Nitrate effects on chromate reduction in a methane-based biofilm.
L. Zhong (2017)
Microbial Cd(II) and Cr(VI) resistance mechanisms and application in bioremediation.
X. Xia (2021)
Chromium-Resistant Bacteria and Their Environmental Condition for Hexavalent Chromium Removal: A Review
M. Narayani (2013)
Genome Sequencing Revealed Chromium and Other Heavy Metal Resistance Genes in E. cloacae B2-Dha
R. Aminur (2017)
Bacterial mechanisms for Cr(VI) resistance and reduction: an overview and recent advances
M. Ahemad (2014)
[Characterization of chromate resistance in genetically engineered Escherichia coli expressing chromate ion transporter ChrA].
Si-min Zhou (2017)
Acetate biostimulation as an effective treatment for cleaning up alkaline soil highly contaminated with Cr(VI)
P. Lara (2016)
Co-remediation of pentachlorophenol and Cr6+ by free and immobilized cells of native Bacillus cereus isolate: Spectrometric characterization of PCP dechlorination products, bioreactor trial and chromate reductase activity
Manikant Tripathi (2013)
Hexavalent chromium removal by a new composite system of dissimilatory iron reduction bacteria Aeromonas hydrophila and nanoscale zero-valent iron
Z. Shi (2019)
A minireplicon of plasmid pBMB26 represents a new typical replicon in the megaplasmids of Bacillus cereus group
P. Wang (2018)
Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
A. Elahi (2018)
Strategies for chromium bioremediation of tannery effluent.
S. Garg (2012)
Chromium resistance strategies and toxicity: what makes Ochrobactrum tritici 5bvl1 a strain highly resistant
P. Morais (2011)
A Novel Subspecies of Staphylococcus aureus from Sediments of Lanzhou Reach of the Yellow River Aerobically Reduces Hexavalent Chromium
Xiaowei Zhang (2013)
Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments
Dragana Tamindžija (2019)
Discerning three novel chromate reduce and transport genes of highly efficient Pannonibacter phragmitetus BB: From genome to gene and protein.
Li-yuan Chai (2018)
Biological Cr(VI) Reduction: Microbial Diversity, Kinetics and Biotechnological Solutions to Pollution
E. Chirwa (2011)
Chromium metabolism characteristics of coexpression of ChrA and ChrT gene.
Ruijia Gu (2020)
Microbial Genomics and Bioremediation of Industrial Wastewater
A. Kapley (2016)
Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment
M. W. Henson (2015)
Bacillus dabaoshanensis sp. nov., a Cr(VI)-tolerant bacterium isolated from heavy-metal-contaminated soil
Xiaowen Cui (2015)
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