Online citations, reference lists, and bibliographies.
← Back to Search

Rapid And Sensitive Detection Of Yersinia Pestis By Lateral-flow Assay In Simulated Clinical Samples

Hui-Ling Hsu, Chuan-Chang Chuang, Chung-Chih Liang, D. Chiao, Hsueh-Ling Wu, Yuping Wu, Fengping Lin, R. Shyu
Published 2018 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
BackgroundYersinia pestis is a contributing agent to the epidemic disease, plague, which killed an estimated 200 million people during historical times. In this study, a rapid, cheap, sensitive, and specific technique, the lateral flow assay (F1 strips), has been successfully developed to detect this pathogen, by using paired monoclonal antibodies (MAbs) against Y. pestis capsule like fraction 1 (F1) protein. Compared with the polyclonal antibody (PAb) based F1 strips, the Mab-based F1 strips have a remarkable increased detection limitation (10 to 100 folds). Furthermore, besides the limitation and specificity evaluation, the application of this F1 strip on simulated clinical samples indicate the LFA can be a good candidate to detect plague.MethodsRecombinant F1 antigen was expressed and purified from a series of works. The various anti-F1 monoclonal antibodies generated from hybridoma cells were screened with the ELISA technique. To evaluate the feasibility of this Y. pestis F1 test strip, the F1 protein/Y. pestis was spiked into simulated clinical samples such as human serum, mouse bronchoalveolar lavage fluids, and mouse blood to mimic natural infection status. Additionally, this technique was applied to detect the Y. pestis in the environment-captured rats, to evaluate the practical usefulness of the strips.ResultsBy using this MAb-based-LFA technique, 4 ng/ml of recombinant F1-protein and 103 CFU/ml of Y. pestis could be detected in less than 10 mins, which is at least 10-folds than that of the PAb format. On the other hand, although various Yersinia strains were applied to the strips, only Y. pestis strain showed a positive result; all other Yersinia species did not produce a positive signal, indicating the high efficiency and specificity of the MAb-based F1-strips.ConclusionBased on our findings, we suggest that the MAb-format-LFA will be valuable as a diagnostic tool for the detection of Y. pestis. This report shows that the F1 strip is sufficient to support not only the detection of plague in simulated clinical samples, but also it may be a good candidate to meet the epidemiological surveillance during an outbreak of the biological warfare.
This paper references
Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.
L. Cao (1995)
Development and testing of a rapid diagnostic test for bubonic and pneumonic plague
S. Chanteau (2003)
Fraction 1 capsular antigen (F1) purification from Yersinia pestis CO92 and from an Escherichia coli recombinant strain and efficacy against lethal plague challenge.
G. Andrews (1996)
An evaluation of Pasteurella pestis fraction-1-specific antibody for the confirmation of plague infections.
T. H. Chen (1966)
A major new test for plague
D. Dennis (2003)
Field evaluation of an IgG anti-F1 ELISA test for the serodiagnosis of human plague in Madagascar
B Rasoamanana (1997)
Rapid detection of Yersinia pestis recombinant fraction 1 capsular antigen
Pei-Yi Tsui (2015)
Plague as a biological weapon: medical and public health management. Working Group on Civilian Biodefense.
T. Inglesby (2000)
Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage.
H. Reynolds (1974)
A new gene of the ƒ1 operon of Y. pestis involved in the capsule biogenesis
A.V. Karlyshev (1992)
A new purification strategy for fraction 1 capsular antigen and its efficacy against Yersinia pestis virulent strain challenge.
T. Wang (2008)
Bronchoalveolar lavage in sarcoidosis.
J. Chrétien (1985)
The Yersinia pestis caf1M1A1 Fimbrial Capsule Operon Promotes Transmission by Flea Bite in a Mouse Model of Bubonic Plague
F. Sebbane (2008)
Comparison of passive haemagglutination and enzyme-linked immunosorbent assay for serodiagnosis of plague.
J. Williams (1982)
Caf1R gene and its role in the regulation of capsule formation of Y. pestis
A. V. Karlyshev (1992)
Bronchoalveolar lavage in sarcoidosis. Semin Respir Crit Care Med
M Drent (2007)
Natural history of plague: perspectives from more than a century of research.
K. L. Gage (2005)
Use of an enzyme-linked immunosorbent assay to measure antigenaemia during acute plague.
J. E. Williams (1984)
A rapid test for detection of mite allergens in homes
A. Tsay (2002)
Bacillus anthracis, Francisella tularensis and Yersinia pestis. The most important bacterial warfare agents — review
M. Pohanka (2009)
Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor
H. Wei (2007)
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
W. Burnette (1981)
Detection of Yersinia pestis in Sputum by Real-Time PCR
C. Loïez (2003)
A dipstick immunoassay to rapidly measure serum oestrone sulfate concentrations in horses.
K. Henderson (2000)
Evaluation of a standardized F1 capsular antigen capture ELISA test kit for the rapid diagnosis of plague.
W. Splettstoesser (2004)
Plague and Other Yersinia Infections
T. Butler (1983)
Field evaluation of an immunoglobulin G anti-F1 enzyme-linked immunosorbent assay for serodiagnosis of human plague in Madagascar.
B. Rasoamanana (1997)
Gold nanoparticle-based lateral flow assay for detection of staphylococcal enterotoxin B
Shyu Rong-Hwa (2010)
World Health Organization. Human plague in 1998 and 1999
Harrison's principles of internal medicine
J. M. Guérit (1999)
Colloidal gold-based immunochromatographic assay for detection of botulinum neurotoxin type B.
D. Chiao (2004)
Role of Fraction 1 Antigen of Yersinia pestis in Inhibition of Phagocytosis
Y. Du (2002)
Gold nanoparticle-based immunochromatographic assay for detection of staphylococcal enterotoxin B
RH Shyu (2010)
The effect of adulterants on urine screen for drugs of abuse: detection by an on-site dipstick device.
R. Wong (2002)
Development, Evaluation, and Application of Lateral-Flow Immunoassay (Immunochromatography) for Detection of Rotavirus in Bovine Fecal Samples
Y. Al-Yousif (2002)
Size exclusion and anion exchange high performance liquid chromatography for characterizing metals bound to marine dissolved organic matter.
Natalia García-Otero (2013)
Comparison of Hand-Held Test Kits, Immunofluorescence Microscopy, Enzyme-Linked Immunosorbent Assay, and Flow Cytometric Analysis for Rapid Presumptive Identification of Yersinia pestis
H. Tomaso (2007)
Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays.
D. Peng (2007)
Technical aspects of bronchoalveolar lavage: recommendations for a standard procedure.
R. Baughman (2007)
Comparison of LFA with PCR and RPLA in detecting SEB from isolated clinical strains of Staphylococcus aureus and its application in food samples.
D. Chiao (2013)
Laboratory diagnosis of plague.
P. Russell (1997)
Yersinia Species, Including Plague
D. Dennis (2010)
K. Meyer (1964)
Bronchoalveolar lavage in idiopathic interstitial lung diseases.
S. Nagai (2007)
Yersinia pestis--etiologic agent of plague.
R. Perry (1997)
Comparison of immunostrips with mouse bioassay and bacterial culture in detecting botulinum toxins in bottles from suspected Taiwan high-speed rail bomber
Chia-Tsui Yeh (2014)
Human plague in 1998 and 1999.
F1 antigenaemia in bubonic plague patients, a marker of gravity and efficacy of therapy.
S. Chanteau (1998)
Prevention of bubonic and pneumonic plague using plant-derived vaccines.
M. L. Alvarez (2010)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar