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Applying Genomic And Proteomic Microarray Technology In Drug Discovery

R. Matson
Published 2004 · Biology

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Omics and Microarrays Revisited The Microarray Format The Omic Era The Role for Gene Expression Microarrays in Drug Discovery Proteomics Today-The Great Challenge The Potential Role for Protein Microarrays in Drug Discovery Future Medicine-Pharmacoproteomics or Pharmacogenomics? Commercial Microarrays In Situ DNA Arrays Ex Situ or Spotted DNA Arrays Comparison of Commercial DNA Microarrays Commercial Protein Arrays Three-Dimensional (3D) and Four-Dimensional (4D) Chips Flow-Thru Biochips Electronic Biochips Supports and Surface Chemistries Substrates Physical Features Surface Chemistries Variation in the Performance of Glass Slide-Based Antibody Microarrays Comparison of Different Surface Chemistries for the Immobilization of Auto-Antigens Click Chemistry as an Immobilization Strategy Oxygen Plasma-Mediated Modification of DVD-R Disks for Tethering of Oligonucleotides Construction of Lipid Bilayer Microarrays Arraying Processes Creating Spotted Microarrays Microarray Printing Mechanisms Microarray Pins Other Approaches Setting Up the Print Run Protocols for Printing Nucleic Acids Protocols for Printing Proteins Newer Methods for Printing Gene Expression: Microarray-Based Applications Applications Demonstrating DNA Microarray Utility Biomedical Research Applications Micro-RNA Array-Based Comparative Genomic Hybridization Protein Microarray Applications Applications Demonstrating Protein Microarray Utility Measuring Microarray Performance Other Microarray Formats Useful for Proteomic Applications Dual Labeling of Targets for Increased Sensitivity and Specificity The Depletion of Highly Abundant Proteins from Serum Deemed Unnecessary Competitive ELISA by Protein Microarray The Issue of Cross-Reactivity in a Protein Microarray Sandwich ELISA Multiplex Assays Multiplex Polymerase Chain Reaction (PCR) Multiplex Lateral Flow Multiplex Bead-Based Assays Multiplex Microarrays Adoption of Multiplex Assays Index
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