Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

Evaluation Of Mucoadhesive PLGA Microparticles For Nasal Immunization

Dilip Pawar, A. Goyal, S. Mangal, N. Mishra, B. Vaidya, Shailja Tiwari, A. K. Jain, S. Vyas
Published 2009 · Medicine, Chemistry

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles.
This paper references
10.1016/S0169-409X(97)00066-5
Large molecule and particulate uptake in the nasal cavity: the effect of size on nasal absorption.
Huang (1998)
10.1016/S0264-410X(02)00686-2
Chitosan microparticles for mucosal vaccination against diphtheria: oral and nasal efficacy studies in mice.
I. M. van der Lubben (2003)
10.1016/S0169-409X(98)00041-6
Evaluation of nano- and microparticle uptake by the gastrointestinal tract.
Delie (1998)
10.1016/j.vaccine.2008.02.058
Surface modified liposomes for nasal delivery of DNA vaccine.
K. Khatri (2008)
10.1016/S0378-5173(98)00367-6
Evaluation of the clearance characteristics of bioadhesive systems in humans.
R. Soane (1999)
10.1016/J.CARBPOL.2008.01.015
Thiolated chitosan coated poly hydroxyethyl methacrylate nanoparticles: Synthesis and characterization
Fatemeh Atyabi (2008)
10.1093/EPIREV/MXJ009
Hepatitis B virus infection: epidemiology and vaccination.
C. Shepard (2006)
10.1016/J.VACCINE.2006.01.011
Strong systemic and mucosal immune responses to surface-modified PLGA microspheres containing recombinant hepatitis B antigen administered intranasally.
K. S. Jaganathan (2006)
10.1038/nm1213
Mucosal immunity and vaccines
J. Holmgren (2005)
10.1016/S0264-410X(02)00191-3
Intranasal immunization with recombinant antigens associated with new cationic particles induces strong mucosal as well as systemic antibody and CTL responses.
Arnaud Debin (2002)
10.1016/0168-3659(90)90133-E
Controlled vaccine release in the gut-associated lymphoid tissues. I. Orally administered biodegradable microspheres target the peyer's patches
J. Eldridge (1990)
10.1016/S0378-5173(01)00602-0
Clearance characteristics of chitosan based formulations in the sheep nasal cavity.
R. Soane (2001)
10.1113/expphysiol.1995.sp003882
Selective transport of microparticles across Peyer's patch follicle‐associated M cells from mice and rats
M. W. Smith (1995)
10.1016/S0264-410X(00)00540-5
A novel microencapsulated peptide vaccine against hepatitis B.
J. S. Moynihan (2001)
10.1211/0022357044418
Development of a single‐dose stabilized poly(d,l‐lactic‐co‐glycolic acid) microspheres‐based vaccine against hepatitis B
K. S. Jaganathan (2004)
10.1016/S0264-410X(03)00078-1
Roles of anti-hemagglutinin IgA and IgG antibodies in different sites of the respiratory tract of vaccinated mice in preventing lethal influenza pneumonia.
R. Ito (2003)
10.1016/j.ejpb.2008.03.004
Permeation enhancer effect of chitosan and chitosan derivatives: comparison of formulations as soluble polymers and nanoparticulate systems on insulin absorption in Caco-2 cells.
A. Sadeghi (2008)
10.1016/0168-3659(85)90050-1
Surface, interfacial and molecular aspects of polymer bioadhesion on soft tissues
N. Peppas (1985)
10.1023/A:1012160102740
Chitosans as Absorption Enhancers for Poorly Absorbable Drugs 2: Mechanism of Absorption Enhancement
N. G. Schipper (2004)
10.1016/J.BIOMATERIALS.2003.08.069
Preparation and characterization of cationic PLGA nanospheres as DNA carriers.
M. Ravi Kumar (2004)
10.1016/0264-410X(95)00011-O
Adjuvants for human vaccines--current status, problems and future prospects.
R. Gupta (1995)
10.1016/S0169-409X(01)00171-5
Chitosan as a novel nasal delivery system for vaccines.
L. Illum (2001)
10.1016/S0168-3659(02)00363-2
Nasal drug delivery--possibilities, problems and solutions.
L. Illum (2003)
10.1023/A:1018967116988
Effect of Chitosan on the Permeability of Monolayers of Intestinal Epithelial Cells (Caco-2)
P. Artursson (2004)
10.1038/nri1439
NALT- versus PEYER'S-patch-mediated mucosal immunity
H. Kiyono (2004)
10.1016/S0168-3659(97)00154-5
Comparison of the effect of different chitosan salts and N-trimethyl chitosan chloride on the permeability of intestinal epithelial cells (Caco-2).
A. Kotzé (1998)
10.1016/S0168-3659(99)00131-5
Effect of degree of quaternization of N-trimethyl chitosan chloride for enhanced transport of hydrophilic compounds across intestinal caco-2 cell monolayers.
M. Thanou (2000)
10.1016/0168-3659(95)00146-8
The potential of mucoadhesive polymers in enhancing intestinal peptide drug absorption. III: Effects of chitosan-glutamate and carbomer on epithelial tight junctions in vitro
G. Borchard (1996)
10.1016/0167-5699(92)90158-4
The role of nasopharyngeal lymphoid tissue.
C. Kuper (1992)
10.1016/s0169-409x(01)00176-4
Nasal vaccination: a non-invasive vaccine delivery method that holds great promise for the future.
L. Illum (2001)
10.3109/10611869609015965
Nasal delivery of vaccines.
A. Almeida (1996)
10.1016/S0169-409X(01)00231-9
Oral drug absorption enhancement by chitosan and its derivatives.
M. Thanou (2001)
10.1016/J.EJPB.2003.09.006
Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice.
A. Vila (2004)
10.1016/J.IMLET.2005.04.002
Mannosylated niosomes as adjuvant-carrier system for oral genetic immunization against hepatitis B.
S. Jain (2005)
10.1016/S0378-5173(03)00377-6
Generation of Toxoplasma gondii GRA1 protein and DNA vaccine loaded chitosan particles: preparation, characterization, and preliminary in vivo studies.
M. Bivas-Benita (2003)
10.1016/S0168-3659(01)00486-2
Design of biodegradable particles for protein delivery.
A. Vila (2002)
10.3109/10611860108997935
Transport of Nanoparticles Across the Rat Nasal Mucosa
J. Brooking (2001)
10.1016/S0144-8617(98)00009-5
Preparation and NMR characterization of highly substitutedN-trimethyl chitosan chloride
A. Sieval (1998)
10.1016/S0264-410X(02)00662-X
Nasal delivery of chitosan-DNA plasmid expressing epitopes of respiratory syncytial virus (RSV) induces protective CTL responses in BALB/c mice.
M. Iqbal (2003)
10.1016/J.ADDR.2005.07.001
The basics and underlying mechanisms of mucoadhesion.
J. Smart (2005)
10.1586/14760584.4.2.185
Nanoparticles as carriers for nasal vaccine delivery
M. Köping-Höggård (2005)
10.1111/j.2042-7158.1993.tb05532.x
Immune Response to Nasal Delivery of Antigenically Intact Tetanus Toxoid Associated with Poly(l‐lactic acid) Microspheres in Rats, Rabbits and Guinea‐pigs
A. Almeida (1993)
10.1016/S0169-409X(01)00167-3
Microparticles for intranasal immunization.
M. Vajdy (2001)
10.1023/A:1016085108889
Gastrointestinal Uptake of Biodegradable Microparticles: Effect of Particle Size
M. P. Desai (2004)
10.3109/10611869409015903
Microsphere absorption by the nasal mucosa of the rat.
H. O. Alpar (1994)
Roles of antihemagglutinin IgA and IgG antibodies in different sites of the respiratory tract of vaccinated mice in preventing lethal in fl uenza pneumonia
R Ito (2003)
10.1016/0378-5173(92)90202-D
Nanosphere and microsphere uptake via Peyer's patches: observation of the rate of uptake in the rat after a single oral dose
P. U. Jani (1992)



This paper is referenced by
Immunity after Intranasal Immunization in Mice
T. Qin (2015)
10.1016/j.ijbiomac.2018.01.073
Rational design and evaluation of HBsAg polymeric nanoparticles as antigen delivery carriers.
H. K. Dewangan (2018)
10.3390/md14050090
Effect of Experimental Parameters on Alginate/Chitosan Microparticles for BCG Encapsulation
L. A. Caetano (2016)
10.1007/s11095-011-0403-z
Surface Characterisation of Bioadhesive PLGA/Chitosan Microparticles Produced by Supercritical Fluid Technology
L. Casettari (2011)
10.3109/21691401.2013.769447
Evaluation of mucoadhesive carrier adjuvant: Toward an oral anthrax vaccine
S. Mangal (2014)
10.1016/j.colsurfb.2015.04.015
Surface modification of PLGA nanoparticles by carbopol to enhance mucoadhesion and cell internalization.
S. Surassmo (2015)
10.1007/s40005-012-0042-3
Evaluation of mucoadhesive nanoparticle based nasal vaccine
D. Krishnakumar (2012)
10.1016/B978-0-323-39981-4.00014-2
Microparticles for Vaccine Delivery
N. Mody (2017)
10.1371/journal.pone.0206324
Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens
T. Alkie (2018)
10.1088/0957-4484/24/24/245101
Mechanisms of chitosan-coated poly(lactic-co-glycolic acid) nanoparticles for improving oral absorption of 7-ethyl-10-hydroxycamptothecin.
Miao Guo (2013)
10.24377/LJMU.T.00004499
PULMONARY DELIVERY OF PNEUMOCCOCAL VACCINE USING NANOCOMPOSITE MICROPARTICLE CARRIERS VIA DRY POWDER INHALATION
Iman M. Alfagih (2015)
10.1201/B20045-16
Overview of Polyester Nanosystems for Nasal Administration
M. Kumar (2017)
10.1016/j.vaccine.2011.07.008
Robust antigen-specific humoral immune responses to sublingually delivered adenoviral vectors encoding HIV-1 Env: association with mucoadhesion and efficient penetration of the sublingual barrier.
William Domm (2011)
10.1016/j.colsurfb.2012.06.038
Poly(ε-caprolactone), Eudragit® RS 100 and poly(ε-caprolactone)/Eudragit® RS 100 blend submicron particles for the sustained release of the antiretroviral efavirenz.
Katia P. Seremeta (2013)
10.1517/17425247.2012.697455
Chitosan-based delivery systems for mucosal vaccines
I. Jabbal‐Gill (2012)
10.1016/j.ijpharm.2016.03.013
Immune response elicited by an intranasally delivered HBsAg low-dose adsorbed to poly-ε-caprolactone based nanoparticles.
S. Jesus (2016)
10.1002/ADV.22077
Surface modification of PLGA nanoparticles using chitosan: Effect of molecular weight, concentration, and degree of deacetylation
Nusaiba K Al-Nemrawi (2018)
10.1021/mp4005029
Formulation and characterization of nanoemulsion intranasal adjuvants: effects of surfactant composition on mucoadhesion and immunogenicity.
Pamela T Wong (2014)
10.4155/tde-2016-0068
Intranasal delivery of nanoparticle-based vaccines.
Nirmal Marasini (2017)
10.1016/j.jsps.2016.12.003
Development and evaluation of Desvenlafaxine loaded PLGA-chitosan nanoparticles for brain delivery
Gui-Feng Tong (2017)
10.1021/bm400406x
Carboxymethyl-chitosan-tethered lipid vesicles: hybrid nanoblanket for oral delivery of paclitaxel.
Nitin Joshi (2013)
10.2147/IJN.S25546
Nebulized liposomal gadobenate dimeglumine contrast formulation for magnetic resonance imaging of larynx and trachea
Xiaohui Wei (2011)
10.1016/j.micpath.2018.08.037
Preparation and evaluation of chitosan nanoparticles containing CtxB antigen against Vibrio cholera.
Niloufar Mirzaee Tabrizi (2018)
10.1016/j.ijpharm.2019.118592
Modified chitosan-based nanoadjuvants enhance immunogenicity of protein antigens after mucosal vaccination.
Genada Sinani (2019)
10.22377/AJP.V11I04.1624
Chitosan-Based Zaleplon Nasal Microparticles
S. Sakhare (2017)
10.1016/j.jim.2020.112791
Rational application of nanoadjuvant for mucosal vaccine delivery system.
Hitesh Kumar Dewangan (2020)
10.1007/s40005-016-0233-4
Quantitative evaluation of mucoadhesive polymers to compare the mucoadhesion
Cho-A Lee (2016)
10.1016/j.tibtech.2018.02.008
Antigen-Specific Tolerization and Targeted Delivery as Therapeutic Strategies for Autoimmune Diseases.
A. Shakya (2018)
10.1016/j.vetimm.2016.07.005
Nasal delivery of chitosan-coated poly(lactide-co-glycolide)-encapsulated honeybee (Apis mellifera) venom promotes Th 1-specific systemic and local intestinal immune responses in weaned pigs.
J. Lee (2016)
10.22059/IJVM.2013.35063
Use of N-trimethyl chitosan for intranasal delivery of DNA encoding M2e-HSP70c in mice
M. Dabaghian (2013)
10.17077/etd.h0z4bv8z
Uptake and distribution of ultrafine nanoparticles and microemulsions from the nasal mucosa
B. C. Bejgum (2017)
10.2217/nnm-2017-0015
Recent developments in nanocarrier-aided mucosal vaccination.
Olga Kammona (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar