← Back to Search
Evaluation Of Chitosan/Alginate Beads Using Experimental Design: Formulation And In Vitro Characterization
S. Takka, A. Gürel
Published 2010 · Medicine, Chemistry
Download PDFAnalyze on Scholarcy
Bovine serum albumin-loaded beads were prepared by ionotropic gelation of alginate with calcium chloride and chitosan. The effect of sodium alginate concentration and chitosan concentration on the particle size and loading efficacy was studied. The diameter of the beads formed is dependent on the size of the needle used. The optimum condition for preparation alginate–chitosan beads was alginate concentration of 3% and chitosan concentration of 0.25% at pH 5. The resulting bead formulation had a loading efficacy of 98.5% and average size of 1,501 μm, and scanning electron microscopy images showed spherical and smooth particles. Chitosan concentration significantly influenced particle size and encapsulation efficiency of chitosan–alginate beads (p < 0.05). Decreasing the alginate concentration resulted in an increased release of albumin in acidic media. The rapid dissolution of chitosan–alginate matrices in the higher pH resulted in burst release of protein drug.
This paper references
Calcium alginate microparticles for oral administration: I: Effect of sodium alginate type on drug release and drug entrapment efficiency.
S. Takka (1999)
Characteristics of polyion complexes of chitosan with sodium alginate and sodium polyacrylate
T. Takahashi (1990)
Alginate in Drug Delivery Systems
H. Tønnesen (2002)
Microcapsules of alginate-chitosan--I. A quantitative study of the interaction between alginate and chitosan.
O. Gåserød (1998)
Peptide and Protein Drug Delivery Systems for Nonparenteral Routes of Administration
Ulrik Rahbek (2012)
Oral Route of Peptide and Protein Drug Delivery
V. Lee (1995)
Chitosan-Alginate Microparticles as a Protein Carrier
G. Coppi (2001)
Formulation and investigation of nicardipine HCl-alginate gel beads with factorial design-based studies.
S. Takka (1998)
Drug release from chitosan–alginate complex beads reinforced by a naturally occurring cross-linking agent
F. L. Mi (2002)
Calcium alginate microparticles for oral administration: II. Effect of formulation factors on drug release and drug entrapment efficiency.
F. Acartürk (1999)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
Peptide and protein drug delivery
V. Lee (1991)
Effect of experimental parameters on the formation of alginate–chitosan nanoparticles and evaluation of their potential application as DNA carrier
K. L. Douglas (2005)
Gastric retentive drug-delivery systems.
S. J. Hwang (1998)
Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan--a review.
M. George (2006)
Microcapsules of alginate-chitosan. II. A study of capsule stability and permeability.
O. Gåserød (1999)
Drug permeation enhancement via buccal route: possibilities and limitations.
S. Şenel (2001)
Evaluation of protein release from chitosan-alginate microcapsules produced using external or internal gelation.
G. Vandenberg (2001)
Controlled release of albumin from chitosan-alginate microcapsules.
A. Polk (1994)
Alternative approach to the preparation of chitosan beads
C. Aral (1998)
Pharmaceutical Formulation Development Of Peptides And Proteins
L. Hovgaard (2009)
Preparation and release behavior of carboxymethylated chitosan/alginate microspheres encapsulating bovine serum albumin
L. Zhang (2004)
Protein release from alginate matrices.
Development of three layered buccal compact containing metoprolol tartrate by statistical optimization technique.
C. Narendra (2005)
Chitosan-sodium alginate nanoparticles as submicroscopic reservoirs for ocular delivery: formulation, optimisation and in vitro characterisation.
S. Motwani (2008)
Evaluation of Alginate/Chitosan nanoparticles as antisense delivery vector: Formulation, optimization and in vitro characterization
Tarane Gazori (2009)
Pharmaceutical Experimental Design
Gareth Lewis (1998)
Chitosan/calcium–alginate beads for oral delivery of insulin
P. Hari (1996)
This paper is referenced by
Development and evaluation of alginate-chitosan gastric floating beads loading with oxymatrine solid dispersion
Yanhua Liu (2016)
Formation of three-dimensional polymer structures through radical and ionic reactions of peroxychitosan
Olga Budishevska (2020)
In vitro and in vivo investigation of low molecular weight heparin–alginate beads for oral administration
Canan Iskenderoğlu (2013)
Chitosan-alginate microcapsules of amoxicillin for gastric stability and mucoadhesion
Saahil Arora (2012)
Gene Therapy Challenges in Arthritis
D. Anne (2011)
MICROENCAPSULATION OF BETA-CAROTENE: CHARACTERIZATION, IN VITRO RELEASE, AND BIOAVAILABILITY by ERIK GLENN DONHOWE
William Kerr (2013)
Electrospun poly(ε-caprolactone) nanofibers for bone regeneration and other biomedical applications
T. Antonio (2011)
Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation
Gastón E Ortiz (2017)
Preparation of Hybrid Alginate-Chitosan Aerogel as Potential Carriers for Pulmonary Drug Delivery.
M. Alnaief (2020)
Alginates: Micro- and Nanoparticles
Marlene Lopes (2015)
Preparation and in vitro in vivo characterization of polyelectrolyte alginate–chitosan complex based microspheres loaded with verapamil hydrochloride for improved oral drug delivery
Xian-Zhao Zhang (2015)
Matrices (re)loaded: Durability, viability, and fermentative capacity of yeast encapsulated in beads of different composition during long‐term fed‐batch culture
Jordan Gulli (2019)
Characterization of polymeric microcapsules containing a low molecular weight peptide for controlled release.
K. Moore (2013)
Rapid Large-Scale Culturing of Microencapsulated Hepatocytes : A Promising Approach for Cell-Based Hepatic Support
Y. Chena ()
Natural polymer based (alginate and chitosan) Microparticles for oral drug delivery
P. Parida (2011)
Influence of Sodium Alginate on Hypoglycemic Activity of Metformin Hydrochloride in the Microspheres Obtained by the Spray Drying
Marta Szekalska (2016)
Rapid large-scale culturing of microencapsulated hepatocytes: a promising approach for cell-based hepatic support.
Y Chen (2014)
An investigation into the effects of pH and material concentration on the morphology of Chitosan-Alginate microspheres prepared using an Ionic gelation techniques
Fashli Razak (2017)
Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
Ameya Sharma (2018)
Recent Advances of Chitosan and Its Derivatives for Novel Applications in Food Science
Y. Luo (2013)
FORMULATION OF COATED POLYMER REINFORCED GELLAN GUM BEADS OF TIZANIDINE HCl USING FRACTIONAL FACTORIAL DESIGN
Nevine Shawky (2012)
Chitosan-alginate membranes accelerate wound healing.
G. Caetano (2015)
A Novel Prototype Device for Microencapsulation of Benznidazole: In Vitro/In Vivo Studies
M. G. Barrera (2020)
Preparation and In Vitro Release of Alginate/Chitosan Microparticles Containing α-Glucosidase Inhibiting Peptide
Wen jie Jiang (2014)
Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with Demineralized Bone Matrix in Chitosan-alginate Beads
B. Sutradhar (2013)
Effect of Experimental Parameters on Alginate/Chitosan Microparticles for BCG Encapsulation
L. A. Caetano (2016)
Enhancements in Alginate Microencapsulation Technology & Impacts on Cell Therapy Development
M. Belhaj (2018)
Development of different drug delivery systems for skin regeneration
Ciências da Saúde (2011)
Hydrolysis of magnetic amylase-imprinted poly(ethylene-co-vinyl alcohol) composite nanoparticles.
Mei-Hwa Lee (2012)
The cause and influence of sequentially assembling higher and lower deacetylated chitosans on the membrane formation of microcapsule.
Guoshuang Zheng (2016)
FORMULATION AND EVALUATION OF CHITOSAN PRAZOSIN BEADS BY IONOTROPIC GELATION METHOD
Behin Sundara Raj (2012)
pH-responsive double network alginate/kappa-carrageenan hydrogel beads for controlled protein release: Effect of pH and crosslinking agent
Selin Sarıyer (2020)See more