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Adiponectin Increases LPL Activity Via RhoA/ROCK-mediated Actin Remodelling In Adult Rat Cardiomyocytes.

Riya Ganguly, K. Schram, X. Fang, Minsuk Kim, B. Rodrigues, F. Thong, G. Sweeney
Published 2011 · Medicine, Biology

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Cardiomyocyte substrate utilization is important in maintaining optimal cardiac function. Adiponectin has been shown to confer cardioprotective effects in part via regulating glucose and fatty acid uptake and oxidation in cardiomyocytes. Here we investigated mechanisms whereby adiponectin mediates a particular metabolic effect by focusing on lipoprotein lipase (LPL), an enzyme that increases free fatty acid availability to the heart by breakdown of chylomicrons and very-low-density lipoproteins in circulation. We used primary adult rat cardiomyocytes and demonstrate that adiponectin increased LPL translocation to the cell surface where it could be released at least partly in its active form, as evidenced by measuring basal and heparin-releasable LPL activity. Furthermore, these effects of adiponectin were mediated via remodeling of the actin cytoskeleton. We quantitatively assessed the filamentous to globular actin ratio and show that increased stress fiber formation, visualized by rhodamine-phalloidin immunofluorescence, in response to adiponectin, is achieved via stimulating Ras homolog gene family A (RhoA) activity, determined using G-LISA RhoA activation assay kit. We also demonstrate that adiponectin induces phosphorylation and inhibition of cofilin, leading to a reduction in actin treadmilling. Increased cofilin phosphorylation and stress fiber formation in response to adiponectin were prevented by inhibition of either RhoA or its downstream kinase Rho-associated protein kinase. Importantly, inhibition of cytoskeletal remodeling prevented adiponectin-stimulated plasma membrane LPL content detected by immunofluorescence and also subsequent LPL activity. In summary, we show that adiponectin mediates actin cytoskeleton remodeling to translocate LPL and allow subsequent activation.
This paper references
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.1007/s00125-004-1429-0
Overexpression of lipoprotein lipase improves insulin resistance induced by a high-fat diet in transgenic rabbits
S. Kitajima (2004)
10.1152/ajpendo.90920.2008
Lipoprotein lipase: from gene to obesity.
H. Wang (2009)
10.1073/PNAS.96.6.3165
Induced mutant mouse lines that express lipoprotein lipase in cardiac muscle, but not in skeletal muscle and adipose tissue, have normal plasma triglyceride and high-density lipoprotein-cholesterol levels.
S. Levak-Frank (1999)
10.1152/ajpendo.90260.2008
Metabolism of very-low-density lipoprotein and chylomicrons by streptozotocin-induced diabetic rat heart: effects of diabetes and lipoprotein preference.
You-guo Niu (2008)
10.1038/ijo.2008.233
Physiological and pathophysiological roles of adiponectin and adiponectin receptors in the integrated regulation of metabolic and cardiovascular diseases
T. Yamauchi (2008)
Acute
MS Kim (2008)
10.1677/JME.1.01877
Hyperglycemia- and hyperinsulinemia-induced alteration of adiponectin receptor expression and adiponectin effects in L6 myoblasts.
X. Fang (2005)
10.1016/S0955-0674(02)00349-6
Regulating the actin cytoskeleton during vesicular transport.
M. Stamnes (2002)
10.1038/nm724
Diet-induced insulin resistance in mice lacking adiponectin/ACRP30
Norikazu Maeda (2002)
10.1055/s-2005-870318
Relationship of lipoprotein lipase and hepatic triacylglycerol lipase activity to serum adiponectin levels in Japanese hyperlipidemic men.
J. Kobayashi (2005)
10.1016/S0008-6363(99)00268-0
The role of the cytoskeleton in heart failure.
S. Hein (2000)
10.1074/JBC.M400347200
gAd-globular Head Domain of Adiponectin Increases Fatty Acid Oxidation in Newborn Rabbit Hearts*
A. Onay-Besikci (2004)
10.1074/JBC.M401028200
Cardiac-specific Knock-out of Lipoprotein Lipase Alters Plasma Lipoprotein Triglyceride Metabolism and Cardiac Gene Expression*
A. Augustus (2004)
10.1016/J.METABOL.2006.03.020
Distinct effects of short- and long-term leptin treatment on glucose and fatty acid uptake and metabolism in HL-1 cardiomyocytes.
R. Palanivel (2006)
10.1016/J.CARDIORES.2007.04.011
Globular and full-length forms of adiponectin mediate specific changes in glucose and fatty acid uptake and metabolism in cardiomyocytes.
R. Palanivel (2007)
10.1093/cvr/cvn034
Adiponectin inhibits vascular endothelial growth factor-induced migration of human coronary artery endothelial cells.
K. Mahadev (2008)
Post-heparin plasma lipoprotein lipase, but not hepatic lipase activity, is related to plasma adiponectin in type 2 diabetic patients and healthy subjects.
R. de Vries (2005)
10.2337/db07-0832
Acute Diabetes Moderates Trafficking of Cardiac Lipoprotein Lipase Through p38 Mitogen-Activated Protein Kinase–Dependent Actin Cytoskeleton Organization
M. Kim (2008)
10.1152/physrev.00017.2007
Cardiac remodeling in obesity.
E. Abel (2008)
10.1210/JC.2007-0890
Total and high molecular weight but not trimeric or hexameric forms of adiponectin correlate with markers of the metabolic syndrome and liver injury in Thai subjects.
Y. Liu (2007)
10.1007/s10557-006-0633-1
Cardiac Metabolism and Mechanics are Altered by Genetic Loss of Lipoprotein Triglyceride Lipolysis
Hye-Lim Noh (2006)
Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1
中村 信久 (2009)
10.2337/db07-0435
Adiponectin Reduces Plasma Triglyceride by Increasing VLDL Triglyceride Catabolism
L. Qiao (2008)
10.1152/ajpendo.00004.2008
Acute dexamethasone-induced increase in cardiac lipoprotein lipase requires activation of both Akt and stress kinases.
G. Kewalramani (2008)
10.1016/j.febslet.2007.11.070
The physiological and pathophysiological role of adiponectin and adiponectin receptors in the peripheral tissues and CNS
T. Kadowaki (2008)
10.1152/AJPENDO.2000.279.5.E1104
Altered metabolism causes cardiac dysfunction in perfused hearts from diabetic (db/db) mice.
D. Belke (2000)
10.2337/DIACARE.27.12.2925
Decreased plasma lipoprotein lipase in hypoadiponectinemia: an association independent of systemic inflammation and insulin resistance.
M. von Eynatten (2004)
10.1161/CIRCRESAHA.107.150417
Cardiac Energy Metabolism in Obesity
G. Lopaschuk (2007)
10.1042/BST0340798
Mechanisms regulating energy metabolism by adiponectin in obesity and diabetes.
X. Fang (2006)
10.1210/JC.2002-021721
Diurnal and ultradian dynamics of serum adiponectin in healthy men: comparison with leptin, circulating soluble leptin receptor, and cortisol patterns.
A. Gavrila (2003)
10.1016/J.CARDIORES.2007.03.009
Acute inhibition of Rho-kinase improves cardiac contractile function in streptozotocin-diabetic rats.
Guorong Lin (2007)
Adiponectin regulates cardiomyocyte fatty acid uptake and oxidation both in primary cells and ex vivo via an APPL1-dependent signaling pathway
X Fang (2010)
10.1242/jcs.02994
Cell surface actin remodeling
T. Stossel (2006)
10.2217/14796678.3.6.647
Potential of adiponectin as a cardioprotective agent.
R. Shibata (2007)
10.1074/JBC.M200601200
Hydroxylation and Glycosylation of the Four Conserved Lysine Residues in the Collagenous Domain of Adiponectin
Y. Wang (2002)
10.1016/J.JMB.2006.10.102
Actin filament severing by cofilin.
D. Pavlov (2007)
10.1152/AJPHEART.01162.2004
Lysophosphatidic acid-mediated augmentation of cardiomyocyte lipoprotein lipase involves actin cytoskeleton reorganization.
T. Pulinilkunnil (2005)
10.1016/j.febslet.2005.07.098
Adiponectin is synthesized and secreted by human and murine cardiomyocytes
R. Piñeiro (2005)
moderates trafficking of cardiac lipoprotein lipase through p 38 mitogen - activated protein kinase - dependent actin cytoskeleton organization
G Kewalramani (2008)



This paper is referenced by
10.1016/j.metabol.2015.11.011
Change in adiponectin explains most of the change in HDL particles induced by lifestyle intervention but not metformin treatment in the Diabetes Prevention Program.
R. Goldberg (2016)
10.3109/10408363.2014.997931
Lipoprotein lipase and angiopoietin-like 4 – Cardiomyocyte secretory proteins that regulate metabolism during diabetic heart disease
P. Puthanveetil (2015)
10.1016/j.bbalip.2014.11.007
Intrinsic and extrinsic regulation of cardiac lipoprotein lipase following diabetes.
Y. Wang (2015)
10.1017/S0029665111003181
Effect of interaction between PPARG, PPARA and ADIPOQ gene variants and dietary fatty acids on plasma lipid profile and adiponectin concentration in a large intervention study.
A. Alsaleh (2012)
10.1210/jc.2013-1452
Lifestyle and metformin treatment favorably influence lipoprotein subfraction distribution in the Diabetes Prevention Program.
R. Goldberg (2013)
10.1016/j.biopha.2016.11.079
Betulin inhibited cigarette smoke-induced COPD in mice.
M. Chun-hua (2017)
10.1177/0004563213487892
Plasma adiponectin is a more specific marker of fatty liver than a marker of metabolic syndrome in Japanese men
Shin-ichi Yatsuzuka (2014)
10.3389/fphar.2015.00276
The Function of Rho-Associated Kinases ROCK1 and ROCK2 in the Pathogenesis of Cardiovascular Disease
Svenja Hartmann (2015)
10.1016/j.addr.2015.07.009
Electrical and mechanical stimulation of cardiac cells and tissue constructs.
Whitney Stoppel (2016)
10.1007/s10741-012-9337-8
Direct effects of adipokines on the heart: focus on adiponectin
M. Park (2012)
10.1139/y2012-053
Adipokines and the cardiovascular system: mechanisms mediating health and disease.
Josette M. Northcott (2012)
10.1111/j.1476-5381.2011.01395.x
Adiponectin and cardiovascular health: an update
Xiaoyan Hui (2012)
10.1007/s12031-019-01402-z
Anfibatide Preserves Blood–Brain Barrier Integrity by Inhibiting TLR4/RhoA/ROCK Pathway After Cerebral Ischemia/Reperfusion Injury in Rat
Peng Gong (2019)
The cardio-protective signaling and mechanisms of adiponectin.
G. Nanayakkara (2012)
10.3389/fendo.2011.00062
Adiponectin Action: A Combination of Endocrine and Autocrine/Paracrine Effects
K. Dadson (2011)
10.1586/eem.11.63
Cardiovascular complications of diabetes: recent insights in pathophysiology and therapeutics
André Marette (2011)
10.1152/ajpheart.00765.2011
Adiponectin and adipocyte fatty acid binding protein in the pathogenesis of cardiovascular disease.
A. Xu (2012)
10.1111/jvim.12433
Effect of Physiological Determinants and Cardiac Disease on Plasma Adiponectin Concentrations in Dogs
C. Damoiseaux (2014)
Modulation of Cardiac Fibroblast to Myofibroblast Transition by Rho-Associated Kinases ROCK1 and ROCK2
Svenja Hartmann (2016)
10.1016/j.phrs.2014.03.003
Back to the heart: the protective role of adiponectin.
C. Caselli (2014)
10.1152/ajpendo.00193.2019
HDL Flux is Higher in Patients with Nonalcoholic Fatty Liver Disease.
A. Mccullough (2019)
10.1007/978-1-62703-770-9_17
Adipokines in Nonalcoholic Fatty Liver Disease
A. Baranova (2014)
10.1016/j.lfs.2019.116955
Rock protein as cardiac hypertrophy modulator in obesity and physical exercise.
Chadi Pellegrini Anaruma (2019)
10.1530/JME-13-0059
Globular adiponectin induces LKB1/AMPK-dependent glucose uptake via actin cytoskeleton remodeling.
V. Vu (2013)
10.1016/j.bone.2016.09.003
Sphingosine-1-phosphate/S1PR2-mediated signaling triggers Smad1/5/8 phosphorylation and thereby induces Runx2 expression in osteoblasts.
Katsumasa Higashi (2016)
10.1155/2013/480597
QiShenYiQi Pills, a Compound Chinese Medicine, Ameliorates Doxorubicin-Induced Myocardial Structure Damage and Cardiac Dysfunction in Rats
Dong-Xin Tang (2013)
10.1016/j.metabol.2014.07.005
Adiponectin stimulates Rho-mediated actin cytoskeleton remodeling and glucose uptake via APPL1 in primary cardiomyocytes.
R. Palanivel (2014)
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