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Inhibition By Licochalcone A, A Novel Flavonoid Isolated From Liquorice Root, Of IL‐1β‐induced PGE2 Production In Human Skin Fibroblasts
Ikue Furuhashi, Susumu Iwata, T. Sato, H. Inoue, S. Shibata
Published 2005 · Chemistry, Medicine
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Licochalcone A, a novel flavonoid isolated from the root of Glycyrrhiza inflata, has been reported to exhibit anti‐inflammatory activity in animal models. In this study, we examined the effect of licochalcone A on the production of chemical mediators such as prostaglandin (PG)E2 and cytokines by interleukin (IL)‐1β in human skin fibroblasts. Licochalcone A (IC50 15.0 nm) inhibited PGE2 production, but not IL‐6 and IL‐8 production, in response to IL‐1β. NS‐398 (IC50 1.6 nm), a COX‐2 selective inhibitor, also suppressed the PGE2 production. Furthermore, licochalcone A and NS‐398 suppressed PGF2α production by IL‐1β. However, licochalcone A (1 μm) had no effect on increased levels of cyclooxygenase (COX)‐2 mRNA and protein in cells. Dexamethasone (100 nm) not only inhibited PGE2, PGF2α, IL‐6 and IL‐8 production but also strongly suppressed the expression of COX‐2 mRNA and protein. Licochalcone A had no effect on COX‐1‐dependent PGE2 production, whereas indometacin (100 nm), a dual inhibitor of COX‐1 and COX‐2, was very effective. These results suggest that licochalcone A induces an anti‐inflammatory effect through the inhibition of COX‐2‐dependent PGE2 production. Furthermore, it appears that the inhibitory effect of licochalcone A on PGE2 production in response to IL‐1β is quite different from that of the steroid.
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