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Free Cytoplasmic Ca++ At Rest And After Cholinergic Stimulus Is Increased In Cultured Muscle Cells From Duchenne Muscular Dystrophy Patients
T. Mongini, D. Ghigo, C. Doriguzzi, F. Bussolino, G. Pescarmona, B. Polio, D. Schiffer, A. Bosìa
Published 1988 · Medicine
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We used a fluorescent dye, quin 2, to measure intracellular free calcium ([Ca+ +]i) in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca+ +]i level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca+ +]i transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.
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