Online citations, reference lists, and bibliographies.
← Back to Search

Gold Nanoparticle Mediated Laser Transfection For Efficient SiRNA Mediated Gene Knock Down

D. Heinemann, M. Schomaker, S. Kalies, M. Schieck, R. Carlson, H. Escobar, T. Ripken, H. Meyer, A. Heisterkamp
Published 2013 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Laser based transfection methods have proven to be an efficient and gentle alternative to established molecule delivery methods like lipofection or electroporation. Among the laser based methods, gold nanoparticle mediated laser transfection bears the major advantage of high throughput and easy usability. This approach uses plasmon resonances on gold nanoparticles unspecifically attached to the cell membrane to evoke transient and spatially defined cell membrane permeabilization. In this study, we explore the parameter regime for gold nanoparticle mediated laser transfection for the delivery of molecules into cell lines and prove its suitability for siRNA mediated gene knock down. The developed setup allows easy usage and safe laser operation in a normal lab environment. We applied a 532 nm Nd:YAG microchip laser emitting 850 ps pulses at a repetition rate of 20.25 kHz. Scanning velocities of the laser spot over the sample of up to 200 mm/s were tested without a decline in perforation efficiency. This velocity leads to a process speed of ∼8 s per well of a 96 well plate. The optimal particle density was determined to be ∼6 particles per cell using environmental scanning electron microscopy. Applying the optimized parameters transfection efficiencies of 88% were achieved in canine pleomorphic adenoma ZMTH3 cells using a fluorescent labeled siRNA while maintaining a high cell viability of >90%. Gene knock down of d2-EGFP was demonstrated and validated by fluorescence repression and western blot analysis. On basis of our findings and established mathematical models we suppose a mixed transfection mechanism consisting of thermal and multiphoton near field effects. Our findings emphasize that gold nanoparticle mediated laser transfection provides an excellent tool for molecular delivery for both, high throughput purposes and the transfection of sensitive cells types.
This paper references
10.1016/J.JPHOTOCHEMREV.2012.01.001
Studies on the interaction of pulsed lasers with plasmonic gold nanoparticles toward light manipulation, heat management, and nanofabrication
S. Hashimoto (2012)
10.1021/NL052396O
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells.
B. Chithrani (2006)
10.1016/j.jconrel.2011.02.006
Selective gene transfection of individual cells in vitro with plasmonic nanobubbles.
E. Lukianova-Hleb (2011)
10.1088/0022-3727/41/18/185501
Thermal Analysis of Gold Nanorods Heated with Femtosecond Laser Pulses.
O. Ekici (2008)
10.18725/OPARU-1914
Implementierung und Anwendung analytischer und numerischer Verfahren zur Lösung der Maxwellgleichungen für die Untersuchung der Lichtausbreitung in biologischem Gewebe
J. Schäfer (2011)
10.1063/1.2766835
Femtosecond cellular transfection using a nondiffracting light beam
X. Tsampoula (2007)
10.1117/12.869054
Ultrashort laser pulse cell manipulation using nano- and micro- materials
M. Schomaker (2010)
10.1364/OE.18.000938
Image patterned molecular delivery into live cells using gold particle coated substrates
Ting-Hsiang Wu (2010)
10.1016/S0091-679X(06)82004-2
Mechanisms of laser cellular microsurgery.
P. Quinto-Su (2007)
10.1002/jbio.201200200
Enhancement of extracellular molecule uptake in plasmonic laser perforation.
S. Kalies (2014)
10.1002/SMLL.200400093
Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity.
E. E. Connor (2005)
10.1117/1.2137321
Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles.
C. Yao (2005)
10.1117/1.3430733
Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation.
P. Mthunzi (2010)
10.1039/b711486a
Modelling the optical response of gold nanoparticles.
V. Myroshnychenko (2008)
10.1098/rsif.2009.0463
Single cell optical transfection
D. Stevenson (2010)
10.1364/JOSAB.5.000648
Laser-induced damage on single-crystal metal surfaces
Y. Jee (1988)
10.1159/000073415
Molecular characterization of the canine HMGB1
H. Murua Escobar (2003)
10.1016/J.JQSRT.2012.05.019
Calculation of the near fields for the scattering of electromagnetic waves by multiple infinite cylinders at perpendicular incidence
J. Schäfer (2012)
10.1002/LAPL.200810072
Photothermal and accompanied phenomena of selective nanophotothermolysis with gold nanoparticles and laser pulses
V. K. Pustovalov (2008)
10.1021/JP057175L
Spectroscopic study of laser-induced phase transition of gold nanoparticles on nanosecond time scales and longer.
S. Inasawa (2006)
10.1002/CHIN.200849210
Modelling the Optical Response of Gold Nanoparticles
V. Myroshnychenko (2008)
10.1364/OE.16.009357
Targeted transfection of stem cells with sub-20 femtosecond laser pulses.
Aisada Uchugonova (2008)
10.1093/JB/MVG132
Elevation of plasma membrane permeability on laser irradiation of extracellular latex particles.
Y. Umebayashi (2003)
10.1016/j.biomaterials.2011.11.062
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells.
J. Baumgart (2012)
10.1088/0508-3443/3/9/307
Heat flow in an infinite medium heated by a sphere
H. Goldenberg (1952)
Transfection by Optical Injection. In: Tuchin VV, Editor. Handbook of Photonics for
Dj Stevenson (2010)
10.1073/pnas.171251798
Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems
N. Caplen (2001)
10.1201/9781439806296-C3
Transfection by Optical Injection
D. Stevenson (2010)
10.1021/nl302200w
Plasma mediated off-resonance plasmonic enhanced ultrafast laser-induced nanocavitation.
É. Boulais (2012)
10.1002/SMLL.200700378
Size-dependent cytotoxicity of gold nanoparticles.
Y. Pan (2007)
10.1364/BOE.1.000587
Influence of laser parameters and staining on femtosecond laser-based intracellular nanosurgery
K. Kuetemeyer (2010)
10.1117/12.809316
Plasmonic perforation of living cells using ultrashort laser pulses and gold nanoparticles
M. Schomaker (2009)
10.1021/nn1000222
Plasmonic nanobubbles as transient vapor nanobubbles generated around plasmonic nanoparticles.
E. Lukianova-Hleb (2010)
10.1103/PHYSREVLETT.80.4249
SURFACE-PLASMON RESONANCES IN SINGLE METALLIC NANOPARTICLES
T. Klar (1998)
10.1016/0021-9797(84)90512-5
Absorption and scattering of light by small particles
P. Barber (1984)
10.1002/jbio.201000052
Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection.
M. Antkowiak (2010)
10.1007/S00340-005-2036-6
Mechanisms of femtosecond laser nanosurgery of cells and tissues
A. Vogel (2005)
Gold Nanoparticle Mediated Laser Transfection PLOS ONE | www.plosone
10.1021/JP984796O
Size and temperature dependence of the plasmon absorption of colloidal gold nanoparticles
S. Link (1999)
10.1103/PHYSREVB.24.649
Local fields at the surface of noble-metal microspheres
B. Messinger (1981)
10.1002/LPOR.200810003
Metal‐nanoparticle plasmonics
M. Pelton (2008)
10.1038/nnano.2010.126
Delivery of molecules into cells using carbon nanoparticles activated by femtosecond laser pulses
Prerona Chakravarty (2010)
10.1016/J.APSUSC.2007.02.108
Nanohole processing on silicon substrate by femtosecond laser pulse with localized surface plasmon polariton
P. A. Atanasov (2007)
Targeted transfection by femtosecond laser
Uk Tirlapur (2002)
10.1364/OE.16.003021
Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53 a cells.
J. Baumgart (2008)
10.1117/1.3253320
Influence of laser parameters on nanoparticle-induced membrane permeabilization.
C. Yao (2009)
10.1038/nmeth.2089
NIH Image to ImageJ: 25 years of image analysis
C. Schneider (2012)
10.1007/s10867-009-9138-z
Mechanisms of laser nanoparticle-based techniques for gene transfection—a calculation study
Chengbo Liu (2009)
Enhancement and mechanisms of extracellular molecule uptake in plasmonic laser perforation
S Kalies (2013)
10.1039/c2lc40708f
High-throughput optical injection of mammalian cells using a Bessel light beam.
H. A. Rendall (2012)
10.1371/journal.pone.0034537
Improved Cellular Specificity of Plasmonic Nanobubbles versus Nanoparticles in Heterogeneous Cell Systems
Ekaterina Y. Lukianova-Hleb (2012)
10.1002/SMLL.200500492
Adverse effects of citrate/gold nanoparticles on human dermal fibroblasts.
N. Pernodet (2006)
10.1007/S11051-010-9976-4
Interaction between ultrashort laser pulses and gold nanoparticles: nanoheater and nanolens effect
N. Nedyalkov (2011)



This paper is referenced by
10.1002/ANDP.201600135
The wavelength dependence of gold nanorod-mediated optical breakdown during infrared ultrashort pulses: The wavelength dependence of gold nanorod-mediated
Yevgeniy R. Davletshin (2017)
10.1371/journal.pone.0079235
Femtosecond Optoinjection of Intact Tobacco BY-2 Cells Using a Reconfigurable Photoporation Platform
C. Mitchell (2013)
10.1117/12.2269948
Cell culture surfaces with immobilized gold nanostars: a new approach for laser-induced plasmonic cell optoporation
Ekaterina Vanzha (2017)
Integration of Gap Junction Coupling in Adenosine Signalling of Endothelial Cells
Von der Naturwissenschaftlichen (2018)
10.1002/jbio.202000017
Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods.
Cuiping Yao (2020)
Both pro-and anti-inflammatory cytokines are up-regulated in the monocytic cell line THP-1 through CCN 1-coating of decellularized equine carotid arteries
J. Lovecchio (2014)
10.1364/BOE.5.002686
Surface modification of silica particles with gold nanoparticles as an augmentation of gold nanoparticle mediated laser perforation
S. Kalies (2014)
10.1007/s10863-015-9623-y
Gold nanoparticle-mediated (GNOME) laser perforation: a new method for a high-throughput analysis of gap junction intercellular coupling
Daniela Begandt (2015)
10.1117/12.2086679
Perspectives in nanostructure assisted laser manipulation of mammalian cells
D. Heinemann (2015)
10.1021/nn5017742
Comparison of gold nanoparticle mediated photoporation: vapor nanobubbles outperform direct heating for delivering macromolecules in live cells.
R. Xiong (2014)
10.1021/nl504497m
Optical injection of gold nanoparticles into living cells.
M. Li (2015)
10.3390/ma9050397
Gold Nanoparticle-Mediated Delivery of Molecules into Primary Human Gingival Fibroblasts Using ns-Laser Pulses: A Pilot Study
Judith Krawinkel (2016)
10.1117/12.2032385
Gold nanoparticle mediated laser transfection for high-throughput antisense applications
S. Kalies (2013)
10.1002/jbio.201500135
Cell perforation mediated by plasmonic bubbles generated by a single near infrared femtosecond laser pulse.
C. Boutopoulos (2016)
10.2116/analsci.31.293
An efficient method is required to transfect non-dividing cells with genetically encoded optical probes for molecular imaging.
M. Awais (2015)
10.1117/1.JBO.20.11.115005
Characterization of the cellular response triggered by gold nanoparticle–mediated laser manipulation
S. Kalies (2015)
10.1364/BOE.8.004756
Analysis of poration-induced changes in cells from laser-activated plasmonic substrates.
Nabiha Saklayen (2017)
10.1533/9781908818782.331
Nanomaterial-assisted light-induced poration and transfection of mammalian cells
Weimeng Ding (2015)
10.1002/jbio.201600146
Structural damage of Bacillus subtilis biofilms using pulsed laser interaction with gold thin films.
Judith Krawinkel (2017)
10.1117/12.2077601
Laser transfection with gold nanoparticles: current state and new particle structures as a perspective
S. Kalies (2015)
10.3390/ijms17081295
Towards Effective Photothermal/Photodynamic Treatment Using Plasmonic Gold Nanoparticles
A. Bucharskaya (2016)
10.1007/978-1-4939-7584-6_9
Influence of External Electrical Stimulation on Cellular Uptake of Gold Nanoparticles
Samantha Franklin (2018)
10.1364/BOE.8.000177
Modulation of cardiomyocyte activity using pulsed laser irradiated gold nanoparticles.
Lara Gentemann (2017)
10.1016/j.tibtech.2016.12.009
Will Nanotechnology Bring New Hope for Gene Delivery?
J. Wong (2017)
10.3390/ijms21218017
Hyperthermia Induced by Gold Nanoparticles and Visible Light Photothermy Combined with Chemotherapy to Tackle Doxorubicin Sensitive and Resistant Colorectal Tumor 3D Spheroids
C. Roma-Rodrigues (2020)
Mechanisms for the light-cell interface in optical neurostimulation
S. Johannsmeier (2016)
10.1038/s41598-018-24908-9
Gold nanoparticle-mediated laser stimulation induces a complex stress response in neuronal cells
S. Johannsmeier (2018)
10.1117/12.2039379
Mechanistic investigations and molecular medicine applications of gold nanoparticle mediated (GNOME) laser transfection
M. Schomaker (2014)
10.1016/j.omtn.2017.05.003
Gold Nanoparticles for BCR-ABL1 Gene Silencing: Improving Tyrosine Kinase Inhibitor Efficacy in Chronic Myeloid Leukemia
R. Vinhas (2017)
10.1002/ADFM.201602036
A Universal Platform for Macromolecular Deliveryinto Cells Using Gold Nanoparticle Layers via the Photoporation Effect
Zhonglin Lyu (2016)
10.1039/c9bm01284b
Photothermal-assisted surface-mediated gene delivery for enhancing transfection efficiency.
H. Zhang (2019)
10.1117/1.JBO.19.7.070505
Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection
S. Kalies (2014)
See more
Semantic Scholar Logo Some data provided by SemanticScholar