Online citations, reference lists, and bibliographies.

EGF Up-Regulates MiR-31 Through The C/EBPβ Signal Cascade In Oral Carcinoma

Wen-cheng Lu, S. L. Kao, Cheng-Chieh Yang, Hsi-Feng Tu, Cheng-Hsien Wu, Kuo-Wei Chang, Shu-Chun Lin
Published 2014 · Medicine, Biology
Cite This
Download PDF
Analyze on Scholarcy
Oral squamous cell carcinoma (OSCC) is one of the most prevalent carcinomas worldwide. MicroRNAs (miRNAs) are short, non-coding RNAs that regulate gene expression and modulate physiological or pathological processes including OSCC carcinogenesis. miR-31 has been found to be up-regulated in OSCC and to act as an oncogenic miRNA. However, the molecular mechanism underlying miR-31 up-regulation in OSCC is still obscure. The activation of epidermal growth factor receptor (EGFR) signaling axis plays key roles in driving oral carcinogenesis. Our screening identified that there is up-regulation of miR-31, miR-181b and miR-222 in OSCC cells following EGF treatment. Subsequent analysis showed that EGF treatment led to AKT activation, which then resulted in miR-31 up-regulation. Moreover, EGF treatment and the AKT activation induced by exogenous expression up-regulated C/EBPβ expression. The miR-31 up-regulation induced by EGF was abrogated by AKT inhibition or by the knockdown of C/EBPβ expression. In OSCC cell subclones stably overexpressing the functional isoform of C/EBPβ, miR-31 expression was up-regulated. Curcumin is a natural ingredient exhibiting anti-cancer potential. It was found that curcumin attenuated AKT activation and the up-regulation of C/EBPβ and miR-31 caused by EGF stimulation in OSCC cells. Lastly, concordance across the expression of EGFR, the expression of C/EBPβ and the expression of miR-31 in OSCC tissues was found. This study describes a novel scenario where the up-regulation of miR-31 expression in OSCC is, at least in part, a consequence of EGFR oncogenic activation. Although the AKT activation and C/EBPβ expression after EGF treatment might not be directly linked, both events are the crucial mediators underlying miR-31 up-regulation in the EGFR signaling axis.
This paper references
Sossey-Alaoui K (2012) miR-31 and its host gene lncRNA LOC554202 are regulated by promoter hypermethylation in triplenegative breast cancer
K Augoff
miR-21 and miR-31 Converge on TIAM1 to Regulate Migration and Invasion of Colon Carcinoma Cells*
Charisa L Cottonham (2010)
MiR-21 is an EGFR-regulated anti-apoptotic factor in lung cancer in never-smokers
Masahiro Seike (2009)
The CCAAT/ enhancer-binding protein beta-2 isoform (CEBPbeta-2) upregulates galectin-7 expression in human breast cancer cells
CG Campion (2014)
Tissue microarray analysis reveals site-specific prevalence of oncogene amplifications in head and neck squamous cell carcinoma.
K. Freier (2003)
Reprogramming of the Tumor Microenvironment by Stromal Pten-regulated miR-320
A. Bronisz (2012)
2011) miR-181 as a putative biomarker for lymph-node metastasis of oral squamous cell carcinoma
Cc Yang
Curcumin induces G2/M arrest and apoptosis in cisplatin-resistant human ovarian cancer cells by modulating akt and p38 mAPK
N. Weir (2007)
miR-181 as a putative biomarker for lymph-node metastasis of oral squamous cell carcinoma.
Cheng-Chieh Yang (2011)
miR-31 ablates expression of the HIF regulatory factor FIH to activate the HIF pathway in head and neck carcinoma.
Chung-Ji Liu (2010)
Increase of microRNA miR-31 level in plasma could be a potential marker of oral cancer.
C-J Liu (2010)
Epidermal growth factor receptor copy number in potentially malignant oral disorders and oral squamous cell carcinoma: a short communication and preliminary study.
Jose Bagan (2012)
The CCAAT/Enhancer-Binding Protein Beta-2 Isoform (CEBPβ-2) Upregulates Galectin-7 Expression in Human Breast Cancer Cells
Carole G. Campion (2014)
Immunohistochemical expression of EGFR and p-EGFR in oral squamous cell carcinomas
Yukihiro Hiraishi (2008)
Quantitative image analysis of immunohistochemical stains using a CMYK color model
N. Pham (2007)
CCAAT/enhancer binding protein-β is a mediator of keratinocyte survival and skin tumorigenesis involving oncogenic Ras signaling
S. Zhu (2001)
miR-196a Overexpression and miR-196a2 Gene Polymorphism Are Prognostic Predictors of Oral Carcinomas
Chung-Ji Liu (2012)
Cigarette Smoke Induces C/EBP-β-Mediated Activation of miR-31 in Normal Human Respiratory Epithelia and Lung Cancer Cells
S. Xi (2010)
miR-134 induces oncogenicity and metastasis in head and neck carcinoma through targeting WWOX gene.
Chung-Ji Liu (2014)
2013) miR-211 promotes the progression of head and neck carcinomas by targeting TGFbetaRII
Th Chu
miR-221 and miR-222 expression increased the growth and tumorigenesis of oral carcinoma cells.
Chun-Ju Yang (2011)
EGF Up-Regulates miR-31 in OSCC PLOS ONE | www.plosone
miR-211 promotes the progression of head and neck carcinomas by targeting TGFβRII.
Ting-Hui Chu (2013)
The role of cetuximab for the treatment of squamous cell carcinoma of the head and neck.
R. Mehra (2008)
High EGFR expression predicts poor prognosis in patients with squamous cell carcinoma of the oral cavity and oropharynx: a TMA-based immunohistochemical analysis.
K. Laimer (2007)
miR-31 and its host gene lncRNA LOC554202 are regulated by promoter hypermethylation in triple-negative breast cancer
Katarzyna Augoff (2011)
CCAAT/enhancer-binding proteins: structure, function and regulation.
D. Ramji (2002)
MicroRNAs in an Oral Cancer Context – from Basic Biology to Clinical Utility
Mike Gorenchtein (2012)
miR-24 up-regulation in oral carcinoma: positive association from clinical and in vitro analysis.
Shu-Chun Lin (2010)
miR-31 is upregulated in oral premalignant epithelium and contributes to the immortalization of normal oral keratinocytes.
Pei-Shih Hung (2014)
Exploiting salivary miR-31 as a clinical biomarker of oral squamous cell carcinoma.
Chung-Ji Liu (2012)
C/EBPβ Modulates the Early Events of Keratinocyte Differentiation Involving Growth Arrest and Keratin 1 and Keratin 10 Expression
S. Zhu (1999)
Keratinocyte proliferation, differentiation, and apoptosis--differential mechanisms of regulation by curcumin, EGCG and apigenin.
S. Balasubramanian (2007)
Programmed cell death 4 loss increases tumor cell invasion and is regulated by miR-21 in oral squamous cell carcinoma
P. Pintor dos Reis (2010)
MicroRNA aberrances in head and neck cancer: pathogenetic and clinical significance
Hsi-Feng Tu (2013)
Tumor suppressor activity of CCAAT/enhancer binding protein alpha is epigenetically down-regulated in head and neck squamous cell carcinoma.
Kristi L. Bennett (2007)
Nuclear STK15 expression is associated with aggressive behaviour of oral carcinoma cells in vivo and in vitro.
Shou-Yen Kao (2010)
MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors.
X. Liu (2010)
MiR-31 is an independent prognostic factor and functions as an oncomir in cervical cancer via targeting ARID1A.
Nan Wang (2014)
Features of Mammalian microRNA Promoters Emerge from Polymerase II Chromatin Immunoprecipitation Data
David L. Corcoran (2009)
Curcumin (diferuloylmethane) alters the expression profiles of microRNAs in human pancreatic cancer cells
M. Sun (2008)
Mature miR-184 as Potential Oncogenic microRNA of Squamous Cell Carcinoma of Tongue
Thian-Sze Wong (2008)
Biological significance of c-erbB family oncogenes in head and neck cancer
S. Rogers (2005)
C/EBPbeta represses p53 to promote cell survival downstream of DNA damage independent of oncogenic Ras and p19(Arf)
SJ Ewing (2008)
2010) miR-31 ablates expression of the HIF regulatory factor FIH to activate the HIF pathway in head and neck carcinoma
Cj Liu
Curcumin upregulates insulin-like growth factor binding protein-5 (IGFBP-5) and C/EBPalpha during oral cancer suppression.
Kuo-Wei Chang (2010)
Association of epidermal growth factor receptor (EGFR) gene copy number amplification with neck lymph node metastasis in areca-associated oral carcinomas.
Wei-Fan Chiang (2008)
Epidermal growth factor receptor biology in head and neck cancer.
Shailaja Kalyankrishna (2006)

This paper is referenced by
MicroRNA expression patterns in oral squamous cell carcinoma: hsa-mir-99b-3p and hsa-mir-100-5p as novel prognostic markers for oral cancer.
M. Jakob (2019)
Systematical analyses of variants in DNase I hypersensitive sites to identify hepatocellular carcinoma susceptibility loci in a Chinese population
Tao Jiang (2017)
Effects of resveratrol, curcumin, berberine and other nutraceuticals on aging, cancer development, cancer stem cells and microRNAs
James A McCubrey (2017)
Function and regulation of microRNA-31 in development and disease.
Nadezda A. Stepicheva (2016)
Orosomucoid 2 inhibits tumor metastasis and is upregulated by CCAAT/enhancer binding protein β in hepatocellular carcinomas
Tao Fang (2015)
Functions and mechanisms of microRNA-31 in human cancers.
T Yu (2018)
MicroRNA meta-signature of oral cancer: evidence from a meta-analysis
Katarina Zeljic (2018)
Reviews of Physiology, Biochemistry and Pharmacology, Vol. 171
Bernd Nilius (2016)
MicroRNAs expression profile in solid and unicystic ameloblastomas
Amaia Setién-Olarra (2017)
MicroRNA-31 upregulation predicts increased risk of progression of oral potentially malignant disorder.
Kai-Feng Hung (2016)
MicroRNA miR-31 targets SIRT3 to disrupt mitochondrial activity and increase oxidative stress in oral carcinoma.
Yu-Yu Kao (2019)
Diagnostic and prognostic relevance of salivary microRNA-21, -125a, -31 and -200a levels in patients with oral lichen planus - a short report
Masoumeh Mehdipour (2018)
TRAIL, Wnt, Sonic Hedgehog, TGFβ, and miRNA Signalings Are Potential Targets for Oral Cancer Therapy
Ammad Ahmad Farooqi (2017)
Curcumin as a MicroRNA Regulator in Cancer: A Review.
Amir Abbas Momtazi (2016)
Salivary extracellular vesicle-associated miRNAs as potential biomarkers in oral squamous cell carcinoma
Chiara Gai (2018)
MicroRNA-211 Enhances the Oncogenicity of Carcinogen-Induced Oral Carcinoma by Repressing TCF12 and Increasing Antioxidant Activity.
Yi-Fen Chen (2016)
MicroRNA regulation of stem cell differentiation and diseases of the bone and adipose tissue: Perspectives on miRNA biogenesis and cellular transcriptome.
Elizabeth C Martin (2016)
miR-134 targets PDCD7 to reduce E-cadherin expression and enhance oral cancer progression.
Shih-Yuan Peng (2018)
Cell-Free microRNAs as Potential Oral Cancer Biomarkers: From Diagnosis to Therapy
Óscar Rapado-González (2019)
MicroRNAs as effective surrogate biomarkers for early diagnosis of oral cancer
M. Cao (2017)
MiR-31-5p-ACOX1 Axis Enhances Tumorigenic Fitness in Oral Squamous Cell Carcinoma Via the Promigratory Prostaglandin E2
Yi-Hsuan Lai (2018)
Predicting the Presence of Oral Squamous Cell Carcinoma Using Commonly Dysregulated MicroRNA in Oral Swirls
Tami Yap (2018)
MicroRNAs and Growth Factors: An Alliance Propelling Tumor Progression
Merav Kedmi (2015)
Targeting of miR‐31/96/182 to the Numb gene during head and neck oncogenesis
Chung-Hsien Chou (2018)
miR-98 suppresses tumor cell growth and metastasis by targeting IGF1R in oral squamous cell carcinoma.
Yu Du (2015)
Curcumin inhibits cancer progression through regulating expression of microRNAs
Siying Zhou (2017)
MiR-31 and miR-143 affect steroid hormone synthesis and inhibit cell apoptosis in bovine granulosa cells through FSHR.
Zhe Zhang (2019)
The increase of oncogenic miRNA expression in tongue carcinogenesis of a mouse model.
Yu-Yu Kao (2015)
Semantic Scholar Logo Some data provided by SemanticScholar