Online citations, reference lists, and bibliographies.

Clinico-Pathological Association Of Delineated MiRNAs In Uveal Melanoma With Monosomy 3/Disomy 3 Chromosomal Aberrations

Nalini Venkatesan, J. R. Kanwar, Perinkulam Ravi Deepa, Vikas Khetan, Tamsyn M. Crowley, Rajeswari Raguraman, Ganesan Sugneswari, Pukhraj Rishi, Viswanathan Natarajan, Jyotirmay Biswas, Subramanian Krishnakumar
Published 2016 · Biology, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
Purpose To correlate the differentially expressed miRNAs with clinico-pathological features in uveal melanoma (UM) tumors harbouring chromosomal 3 aberrations among South Asian Indian cohort. Methods Based on chromosomal 3 aberration, UM (n = 86) were grouped into monosomy 3 (M3; n = 51) and disomy 3 (D3; n = 35) by chromogenic in-situ hybridisation (CISH). The clinico-pathological features were recorded. miRNA profiling was performed in formalin fixed paraffin embedded (FFPE) UM samples (n = 6) using Agilent, Human miRNA microarray, 8x15KV3 arrays. The association between miRNAs and clinico-pathological features were studied using univariate and multivariate analysis. miRNA-gene targets were predicted using Target-scan and MiRanda database. Significantly dys-regulated miRNAs were validated in FFPE UM (n = 86) and mRNAs were validated in frozen UM (n = 10) by qRT-PCR. Metastasis free-survival and miRNA expressions were analysed by Kaplen-Meier analysis in UM tissues (n = 52). Results Unsupervised analysis revealed 585 differentially expressed miRNAs while supervised analysis demonstrated 82 miRNAs (FDR; Q = 0.0). Differential expression of 8 miRNAs: miR-214, miR-149*, miR-143, miR-146b, miR-199a, let7b, miR-1238 and miR-134 were studied. Gene target prediction revealed SMAD4, WISP1, HIPK1, HDAC8 and C-KIT as the post-transcriptional regulators of miR-146b, miR-199a, miR-1238 and miR-134. Five miRNAs (miR-214, miR146b, miR-143, miR-199a and miR-134) were found to be differentially expressed in M3/ D3 UM tumors. In UM patients with liver metastasis, miR-149* and miR-134 expressions were strongly correlated. Conclusion UM can be stratified using miRNAs from FFPE sections. miRNAs predicting liver metastasis and survival have been identified. Mechanistic linkage of de-regulated miRNA/mRNA expressions provide new insights on their role in UM progression and aggressiveness.
This paper references
10.1007/s11427-011-4149-y
The miRNA expression profile of the uveal melanoma
Cheng-Hsun Yang (2011)
10.1167/iovs.09-4739
Regulation of VEGF-A in uveal melanoma.
Mariam el Filali (2010)
10.1074/jbc.a112.374611
MicroRNA MiR-214 regulates ovarian cancer cell stemness by targeting p53/Nanog.
Cheng-Xiong Xu (2016)
HIPK 1 drives p 53 activation to limit colorectal cancer cell growth
C Rey (2013)
10.1002/ijc.28358
miR-134 induces oncogenicity and metastasis in head and neck carcinoma through targeting WWOX gene.
Chung-Ji Liu (2014)
10.1007/s11010-010-0700-6
miR-143 decreases prostate cancer cells proliferation and migration and enhances their sensitivity to docetaxel through suppression of KRAS
Bin Xu (2010)
10.1001/jamaophthalmol.2015.1790
Variable Results for Uveal Melanoma-Specific Gene Expression Profile Prognostic Test in Choroidal Metastasis.
M. Klufas (2015)
10.1007/978-1-62703-727-3_22
A prognostic test to predict the risk of metastasis in uveal melanoma based on a 15-gene expression profile.
J. William Harbour (2014)
10.1038/eye.2012.126
Progress in the management of patients with uveal melanoma. The 2012 Ashton Lecture
Bertil E. Damato (2012)
Clinical and histopathological characteristics of uveal melanoma in Asian Indians. A study of 103 patients.
Jyotirmay Biswas (2004)
10.1111/aos.12322
MicroRNA expression analysis and Multiplex ligation-dependent probe amplification in metastatic and non-metastatic uveal melanoma.
Ann-Cathrine Larsen (2014)
Concomitant loss of chromosome 3 and whole arm losses and gains of chromosome 1, 6, or 8 in metastasizing primary uveal melanoma.
Y. Aalto (2001)
10.1016/j.ccr.2011.10.030
β-catenin signaling controls metastasis in Braf-activated Pten-deficient melanomas.
William Damsky (2011)
10.1167/iovs.14-15250
Skewed expression of the genes encoding epigenetic modifiers in high-risk uveal melanoma.
Naoimh Herlihy (2015)
10.1586/erm.10.11
Micromarkers: miRNAs in cancer diagnosis and prognosis
Manuela Ferracin (2010)
10.1073/PNAS.0709313104
MicroRNA expression signatures accurately discriminate acute lymphoblastic leukemia from acute myeloid leukemia
Shuangli Mi (2007)
TGF-beta1/SMAD signaling pathway mediates p53-dependent apoptosis in hepatoma cell lines.
Chun-lei Wang (2006)
10.4103/0301-4738.128618
Brachytherapy of intra ocular tumors using ‘BARC I-125 Ocu-Prosta seeds’: An Indian experience
V. Khetan (2014)
10.1261/RNA.642907
Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples.
Y. Xi (2007)
expres - sion of RNA extracted from fresh frozen and formalin - fixed paraffin - embedded samples
DR Ciocca (2005)
10.1371/journal.pone.0083398
Targeted Expression of Suicide Gene by Tissue-Specific Promoter and MicroRNA Regulation for Cancer Gene Therapy
Ravikanth Danda (2013)
10.1158/0008-5472.CAN-09-1298
Up-regulation of miR-200 and let-7 by natural agents leads to the reversal of epithelial-to-mesenchymal transition in gemcitabine-resistant pancreatic cancer cells.
Yiwei Li (2009)
10.1111/pcmr.12156
Identification of glycogen synthase kinase 3α as a therapeutic target in melanoma.
SubbaRao V Madhunapantula (2013)
10.1002/ijc.2910540107
Expression of the neuroglandular antigen and analogues in melanoma. CD9 expression appears inversely related to metastatic potential of melanoma.
Z D Si (1993)
10.4161/cc.24927
HIPK1 drives p53 activation to limit colorectal cancer cell growth
Christophe Rey (2013)
10.1167/iovs.09-3392
Characterizing the involvement of the nuclear factor-kappa B (NF kappa B) transcription factor in uveal melanoma.
Rinat Dror (2010)
10.1038/sj.onc.1206474
SASH1: a candidate tumor suppressor gene on chromosome 6q24.3 is downregulated in breast cancer
Constanze Zeller (2003)
10.1700/1430.15828
Identification of microRNA Expression Patterns in Cutaneous and Uveal Melanoma Cell Lines
Mario Venza (2014)
10.1379/CSC-99r.1
Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implications
Daniel Ramón Ciocca (2005)
10.1371/currents.eogt.af8ba80fc776c8f1ce8f5dc485d4a618
The DecisionDx-UM Gene Expression Profile Test Provides Risk Stratification and Individualized Patient Care in Uveal Melanoma
J. William Harbour (2013)
10.1167/iovs.08-2279
Chromosome 3 intratumor heterogeneity in uveal melanoma.
Hanneke W. Mensink (2009)
TGF-beta1/SMAD signaling pathway mediates p53-dependent apoptosis in hepatoma cell lines.
Wang Cl (2006)
10.1074/jbc.M112.374611
MicroRNA MiR-214 Regulates Ovarian Cancer Cell Stemness by Targeting p53/Nanog*
Cheng-Xiong Xu (2012)
10.1002/hep.23008
Up-regulated microRNA-143 transcribed by nuclear factor kappa B enhances hepatocarcinoma metastasis by repressing fibronectin expression.
X. Zhang (2009)
10.1158/0008-5472.CAN-04-1750
Gene Expression Profiling in Uveal Melanoma Reveals Two Molecular Classes and Predicts Metastatic Death
M. Onken (2004)
10.1111/j.1755-3768.2010.02038.x
HSP-27 protein expression in uveal melanoma: correlation with predicted survival.
Fidan Jmor (2012)
10.1371/journal.pone.0035295
Germline BAP1 Inactivation Is Preferentially Associated with Metastatic Ocular Melanoma and Cutaneous-Ocular Melanoma Families
Ching-Ni Jenny Njauw (2012)
10.1097/CMR.0b013e3282feeac6
Micro-RNAs associated with metastasis in uveal melanoma identified by multiplexed microarray profiling
L. Worley (2008)
10.1016/S0002-9440(10)64817-1
Deregulation of the Rb and p53 pathways in uveal melanoma.
Milam Brantley (2000)
10.1016/j.canlet.2008.08.016
HDAC family: What are the cancer relevant targets?
O. Witt (2009)
10.1097/ICU.0000000000000051
Recent developments in prognostic and predictive testing in uveal melanoma
Matthew Gibson Field (2014)
10.1056/NEJMoa1000584
Mutations in GNA11 in uveal melanoma.
Catherine D Van Raamsdonk (2010)
Analysis of chromosomal aberration (1, 3, and 8) and association of microRNAs in uveal melanoma
Abhirami Radhakrishnan (2009)
10.3332/ecancer.2014.410
Expression of the metastasis suppressor BRMS1 in uveal melanoma
Bruna Vieira Ventura (2014)
10.1101/gad.942902
WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase.
F. Su (2002)
10.1001/archopht.119.5.670
Assessment of metastatic disease status at death in 435 patients with large choroidal melanoma in the Collaborative Ocular Melanoma Study (COMS): COMS report no. 15.
D. Albert (2001)
10.1158/0008-5472.CAN-07-2488
MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN.
H. Yang (2008)
10.1016/j.addr.2014.09.001
Circulating miRNAs: roles in cancer diagnosis, prognosis and therapy.
Guofeng Cheng (2015)



This paper is referenced by
10.2147/OTT.S253946
A Review of MicroRNA in Uveal Melanoma
Yi fan Li (2020)
10.1007/978-981-15-0395-5_7
Genetics of Uveal Melanoma
Helen Kalirai (2020)
10.1177/2472630318819240
Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells
Wenjun Guo (2019)
10.1097/ICU.0000000000000366
New concepts in the molecular understanding of uveal melanoma
David Alan Reichstein (2017)
10.1186/s12913-018-3337-x
Evaluating the implementation related challenges of Shasthyo Suroksha Karmasuchi (health protection scheme) of the government of Bangladesh: a study protocol
Sayem Ahmed (2018)
1 The circulating transcriptome as a source of 2 biomarkers for melanoma 3
Carla Solé (2018)
10.4103/ijo.IJO_49_16
Updates in ophthalmic pathology
Pia R. Mendoza (2017)
10.1002/ijc.31638
miR-134 targets PDCD7 to reduce E-cadherin expression and enhance oral cancer progression.
Shih-Yuan Peng (2018)
10.1186/s41182-019-0137-x
Women’s knowledge on common non-communicable diseases and nutritional need during pregnancy in three townships of Ayeyarwaddy region, Myanmar: a cross-sectional study
Moe Moe Thandar (2019)
10.7150/jca.21044
miR-149 in Human Cancer: A Systemic Review
Yunjie He (2018)
10.3892/or.2017.5537
Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway
Ming Bai (2017)
10.1016/j.omtn.2016.11.003
miR-134: A Human Cancer Suppressor?
Jingyu Pan (2017)
10.3390/cancers11010070
The Circulating Transcriptome as a Source of Biomarkers for Melanoma
Carla Solé (2019)
10.3390/cancers12061468
Potential Onco-Suppressive Role of miR122 and miR144 in Uveal Melanoma through ADAM10 and C-Met Inhibition
Adriana Amaro (2020)
10.7150/ijbs.18331
Role of Epigenetics in Uveal Melanoma
Yongyun Li (2017)
10.18632/oncotarget.27559
Changes in microRNA expression associated with metastasis and survival in patients with uveal melanoma
Ayushi Vashishtha (2020)
10.1080/14737159.2020.1696194
Circulating microRNAs as biomarkers for early diagnosis of cutaneous melanoma
Sara Carpi (2019)
10.1111/cpr.12465
Regulation and functions of MicroRNA‐149 in human cancers
Yingru Zhi (2018)
10.1371/journal.pone.0207435
Choroidal structural analysis in eyes with diabetic retinopathy and diabetic macular edema—A novel OCT based imaging biomarker
Chanda Gupta (2018)
Semantic Scholar Logo Some data provided by SemanticScholar