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Baicalein Inhibits Staphylococcus Aureus Biofilm Formation And The Quorum Sensing System In Vitro

Y. Chen, Tangjuan Liu, Ke Wang, Changchun Hou, Shuang-qi Cai, Yingying Huang, Zhong-ye Du, Hong Huang, J. Kong, Yiqiang Chen
Published 2016 · Biology, Medicine

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Biofilm formed by Staphylococcus aureus significantly enhances antibiotic resistance by inhibiting the penetration of antibiotics, resulting in an increasingly serious situation. This study aimed to assess whether baicalein can prevent Staphylococcus aureus biofilm formation and whether it may have synergistic bactericidal effects with antibiotics in vitro. To do this, we used a clinically isolated strain of Staphylococcus aureus 17546 (t037) for biofilm formation. Virulence factors were detected following treatment with baicalein, and the molecular mechanism of its antibiofilm activity was studied. Plate counting, crystal violet staining, and fluorescence microscopy revealed that 32 μg/mL and 64 μg/mL baicalein clearly inhibited 3- and 7-day biofilm formation in vitro. Moreover, colony forming unit count, confocal laser scanning microscopy, and scanning electron microscopy showed that vancomycin (VCM) and baicalein generally enhanced destruction of biofilms, while VCM alone did not. Western blotting and real-time quantitative polymerase chain reaction analyses (RTQ-PCR) confirmed that baicalein treatment reduced staphylococcal enterotoxin A (SEA) and α-hemolysin (hla) levels. Most strikingly, real-time qualitative polymerase chain reaction data demonstrated that 32 μg/mL and 64 μg/mL baicalein downregulated the quorum-sensing system regulators agrA, RNAIII, and sarA, and gene expression of ica, but 16 μg/mL baicalein had no effect. In summary, baicalein inhibited Staphylococcus aureus biofilm formation, destroyed biofilms, increased the permeability of vancomycin, reduced the production of staphylococcal enterotoxin A and α-hemolysin, and inhibited the quorum sensing system. These results support baicalein as a novel drug candidate and an effective treatment strategy for Staphylococcus aureus biofilm-associated infections.
This paper references
Drug metabolism and disposition : the biological fate of chemicals.
Experimental Therapeutics (1973)
Regulation of exoprotein gene expression in Staphylococcus aureus by agar.Molecular & general genetics:MGG
P Recsei (1986)
10.1046/j.1365-2958.1997.4251782.x
Quorum sensing by peptide pheromones and two‐component signal‐transduction systems in Gram‐positive bacteria
M. Kleerebezem (1997)
10.1016/S0168-1605(98)00050-6
Detection and analysis of Staphylococcal enterotoxin A in food by Western immunoblotting.
A. Rasooly (1998)
10.1126/SCIENCE.280.5362.438
Autoinducer of virulence as a target for vaccine and therapy against Staphylococcus aureus.
N. Balaban (1998)
Detection and analysis of Staphylococcal enterotoxin A in food byWestern immunoblotting
A Rasooly (1998)
10.1128/IAI.67.10.5427-5433.1999
The Intercellular Adhesion (ica) Locus Is Present in Staphylococcus aureus and Is Required for Biofilm Formation
S. Cramton (1999)
10.1074/JBC.M005446200
Regulation of Staphylococcus aureus Pathogenesis via Target of RNAIII-activating Protein (TRAP)*
N. Balaban (2001)
10.1128/CMR.15.2.155-166.2002
Bacterial Adhesion: Seen Any Good Biofilms Lately?
W. M. Dunne (2002)
10.1128/CMR.15.2.167-193.2002
Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms
R. Donlan (2002)
10.1172/JCI20195
Interspecies communication in bacteria.
M. J. Federle (2003)
10.1007/BF00330517
Regulation of exoprotein gene expression in Staphylococcus aureus by agr
P. Recsei (2004)
10.1128/JB.186.14.4665-4684.2004
Global gene expression in Staphylococcus aureus biofilms.
K. Beenken (2004)
10.1159/000086704
Characterizing the Dynamics of the Quorum-Sensing System in Staphylococcus aureus
E. Gustafsson (2004)
10.1111/j.1462-5822.2004.00401.x
Regulated expression of pathogen‐associated molecular pattern molecules in Staphylococcus epidermidis: quorum‐sensing determines pro‐inflammatory capacity and production of phenol‐soluble modulins
C. Vuong (2004)
10.1016/J.MCP.2003.07.009
Quantification of virulence-associated gene transcripts in epidemic methicillin resistant Staphylococcus aureus by real-time PCR.
S. Sabersheikh (2004)
Projan SJ, et al.Global Gene Expression in Staphylococcus aureus Biofilms
KE Beenken (2004)
10.1016/J.FEMSRE.2005.03.001
Inter-kingdom signaling: deciphering the language of acyl homoserine lactones.
E. K. Shiner (2005)
10.1016/J.TIM.2004.11.007
Sociomicrobiology: the connections between quorum sensing and biofilms.
M. Parsek (2005)
10.1111/J.1574-6968.2005.00001.X
Quorum sensing: the many languages of bacteria.
Nicola C. Reading (2006)
10.1016/J.BMC.2006.01.057
Baicalein is a potent in vitro inhibitor against both reticulocyte 15-human and platelet 12-human lipoxygenases.
Joshua D Deschamps (2006)
10.1128/AEM.00198-06
Staphylococcus aureus and Staphylococcal Enterotoxin A in Breaded Chicken Products: Detection and Behavior during the Cooking Process
O. Pepe (2006)
Staphylococcus aureus and staphylococcal enterotoxin A in breaded chicken products: detection and behavior during the cooking process. Applied and environmental microbiology
O Pepe (2006)
黄芩水煎液对生物被膜的抑制作用及对左氧氟沙星的增效作用
孔晋亮 (2007)
10.1186/1476-7961-5-5
Baicalein inhibits IL-1β- and TNF-α-induced inflammatory cytokine production from human mast cells via regulation of the NF-κB pathway
Chia-Jung Hsieh (2007)
Baicalein inhibits IL-1beta- and TNF-alphainduced inflammatory cytokine production from humanmast cells via regulation of the NF-kappaB pathway.Clinical and molecular allergy: CMA
CJ Hsieh (2007)
The inhibition of Radix Scutellariae decoction to the biofilm and its synergistic effect of levofloxacin
JL Kong (2007)
Baicalein inhibits IL - 1 beta - and TNF - alpha - induced inflammatory cytokine production from humanmast cells via regulation of the NF - kappaB path
CJ Hsieh (2007)
Baicalein inhibits IL-1beta-and TNF-alphainduced inflammatory cytokine production from human mast cells via regulation of the NF-kappaB pathway
C J Hsieh (2007)
Baicalein inhibits IL-1beta-and TNF-alphainduced inflammatory cytokine production from human mast cells via regulation of the NF-kappaB pathway
Plos One | Doi Baicalein Inhibits Staphylococcus Aureus Biofilm (2007)
10.1099/mic.0.2007/016014-0
agr RNAIII divergently regulates glucose-induced biofilm formation in clinical isolates of Staphylococcus aureus.
L. R. Coelho (2008)
10.1124/mol.107.044164
Discovery of a Quorum-Sensing Inhibitor of Drug-Resistant Staphylococcal Infections by Structure-Based Virtual Screening
M. Kiran (2008)
10.1007/s00253-008-1406-5
Virtual screening for novel quorum sensing inhibitors to eradicate biofilm formation of Pseudomonas aeruginosa
Z. Zeng (2008)
10.1371/journal.pbio.0060014
The Evolution of Quorum Sensing in Bacterial Biofilms
Carey D. Nadell (2008)
10.1016/j.ijantimicag.2008.06.030
Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titanium medical devices.
S. Fujimura (2008)
10.1128/IAI.00859-08
SarZ Promotes the Expression of Virulence Factors and Represses Biofilm Formation by Modulating SarA and agr in Staphylococcus aureus
S. Tamber (2008)
10.1016/j.bmc.2008.04.067
Baicalin, a prodrug able to reach the CNS, is a prolyl oligopeptidase inhibitor.
T. Tarragó (2008)
Discovery of a quorumsensing inhibitor of drug-resistant staphylococcal infections by structure-based virtual screening
MD Kiran (2008)
Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titaniummedical devices. International journal of antimicrobial agents
S Fujimura (2008)
10.1124/dmd.108.023416
Mechanism of CYP2C9 Inhibition by Flavones and Flavonols
D. Si (2009)
10.1016/j.jep.2011.09.014
Baicalein, isolated from Scutellaria baicalensis, protects against endothelin-1-induced pulmonary artery smooth muscle cell proliferation via inhibition of TRPC1 channel expression.
Y. Lin (2011)
10.1248/BPB.34.253
Antidepressant effects of a plant-derived flavonoid baicalein involving extracellular signal-regulated kinases cascade.
Zhe Xiong (2011)
10.1371/journal.pone.0028737
Ellagic Acid Derivatives from Rubus ulmifolius Inhibit Staphylococcus aureus Biofilm Formation and Improve Response to Antibiotics
C. Quave (2012)
Detection and analysis of Staphylococcal enterotoxin A in food by Western immunoblotting. International journal of food microbiology
A Rasooly
Interspecies communication in bacteria. The Journal of clinical investigation
M J Federle



This paper is referenced by
10.1016/J.MSEC.2020.111537
Baicalein-modified hydroxyapatite nanoparticles and coatings with antibacterial and antioxidant properties.
Estelle Palierse (2021)
10.3390/antibiotics10020215
Baicalein Inhibits Streptococcus mutans Biofilms and Dental Caries-Related Virulence Phenotypes
Aparna Vijayakumar (2021)
10.1080/08927014.2021.1874366
2-Aminoimidazoles as potent inhibitors of contaminating brewery biofilms
Lene Jacobs (2021)
10.3390/ani11041039
The Use of Chinese Skullcap (Scutellaria baicalensis) and Its Extracts for Sustainable Animal Production
Baishuang Yin (2021)
10.3390/antiox10020188
The Interactions between Polyphenols and Microorganisms, Especially Gut Microbiota
M. Makarewicz (2021)
10.3390/molecules25225431
A Review of the Role of Flavonoids in Peptic Ulcer (2010–2020)
Catarina Serafim (2020)
10.1016/j.livsci.2020.104307
Effects of dietary supplementation of enzymatic bio-conversion of Scutellaria baicalensis extract as an alternative to antibiotics on the growth performance, nutrient digestibility, fecal microbiota, fecal gas emission, blood hematology, and antioxidative indicators in growing pigs
D. Dang (2020)
10.1016/j.micpath.2020.104411
Antibacterial synergy between linezolid and baicalein against methicillin-resistant Staphylococcus aureus biofilm in vivo.
Tangjuan Liu (2020)
10.1007/978-981-15-4890-1_17
Plant Phenolics for Overcoming Multidrug Resistance in Human Fungal Pathogen
Shweta Singh (2020)
10.3390/antibiotics9060362
N-Nonyloxypentyl-l-Deoxynojirimycin Inhibits Growth, Biofilm Formation and Virulence Factors Expression of Staphylococcus aureus
E. De Gregorio (2020)
10.33448/rsd-v9i11.9362
Pitanga and grumixama extracts: antioxidant and antimicrobial activities and incorporation into cellulosic films against Staphylococcus aureus
Franciele Mendes de Carvalho (2020)
10.1016/j.jep.2020.112669
Callistemon citrinus bioactive metabolites as new inhibitors of methicillin-resistant Staphylococcus aureus biofilm formation.
Amr Shehabeldine (2020)
10.3390/molecules25215024
Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms
Francesca Guzzo (2020)
10.1016/j.lwt.2020.109668
Phloretin inhibits biofilm formation by affecting quorum sensing under different temperature
Li Na Wei (2020)
10.1016/j.micpath.2020.104056
Antimicrobial mechanism of luteolin against Staphylococcus aureus and Listeria monocytogenes and its antibiofilm properties.
Weidong Qian (2020)
10.3906/sag-2001-123
Baicalein Nanofiber Scaffold Containing Hyaluronic Acid and Polyvinyl Alcohol: Preparation and Evaluation
Kamel Bachimam (2020)
10.1007/978-3-030-03077-3
Biofilm, Pilonidal Cysts and Sinuses
M. Shiffman (2020)
10.24205/03276716.2020.1104
Baicalein Inhibits Migration of Breast Cancer Via Inflammatory and Apoptosis Cell Signaling Pathway
Xue Qifeng (2020)
10.3390/microorganisms8010099
Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
Zhongwei Yuan (2020)
10.1016/j.foodcont.2020.107154
Inhibition of multidrug-resistant foodborne Staphylococcus aureus biofilms by a natural terpenoid (+)-nootkatone and related molecular mechanism
A. K. Farha (2020)
10.1016/j.bcp.2020.114024
Baicalein mediates protection against Staphylococcus aureus-induced pneumonia by inhibiting the coagulase activity of vWbp.
Haitao Zhang (2020)
10.3390/biomedicines8060160
New Pyrimidinone-Fused 1,4-Naphthoquinone Derivatives Inhibit the Growth of Drug Resistant Oral Bacteria
Kyungmin Kim (2020)
10.15190/d.2019.13
Communication is the key: biofilms, quorum sensing, formation and prevention
V. Preda (2019)
10.1016/j.jbiosc.2019.11.008
Antibacterial and antibiofilm activity of ursolic acid against carbapenem-resistant Enterobacter cloacae.
Weidong Qian (2019)
10.1128/IAI.00530-19
The Impacts of msaABCR on sarA-Associated Phenotypes Are Different in Divergent Clinical Isolates of Staphylococcus aureus
Joseph S. Rom (2019)
10.18632/aging.102371
Baicalein attenuates OVA-induced allergic airway inflammation through the inhibition of the NF-κB signaling pathway
Tingting Xu (2019)
10.1016/j.ijmm.2018.11.002
An update on recent developments in the prevention and treatment of Staphylococcus aureus biofilms.
M. Suresh (2019)
10.1002/ptr.6208
Antibacterial activity of flavonoids and their structure–activity relationship: An update review
F. Farhadi (2019)
10.3390/ijms20215307
Detection and Quantification of eDNA-Associated Bacterial Membrane Vesicles by Flow Cytometry
Valentina Puca (2019)
10.1021/acs.jafc.9b05595
Natural products that target virulence factors in antibiotic resistant Staphylococcus aureus.
Shuai-Cheng Wu (2019)
10.3390/molecules24101937
Inhibitory Effects on Clinical Isolated Bacteria and Simultaneous HPLC Quantitative Analysis of Flavone Contents in Extracts from Oroxylum indicum
Patchima Sithisarn (2019)
10.1016/j.micpath.2018.11.039
Antimicrobial, anti-adhesive and anti-biofilm potential of biosurfactants isolated from Pediococcus acidilactici and Lactobacillus plantarum against Staphylococcus aureus CMCC26003.
Xin Yan (2019)
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