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A New Interpretation Of The Necrotic Changes Occurring In The Developing Limb Bud Paddle Of Mouse Embryos Based Upon Recent Observations In Four Different Phenotypes.

J. Milaire
Published 1992 · Biology, Medicine

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Degenerative changes occurring in the apical ectodermal ridge (a.e.r.) and undifferentiated distal mesoderm of developing limb buds were studied macro- and microscopically in day-11 to day-13 mouse embryos displaying the normal (+/+), oligosyndactylous (Os/+), polydactylous (Xpl/+) and hybrid (Os/+/Xpl/+) phenotypes. Isolated limb buds were submitted either to supravital staining with Nile blue sulfate or to lectin binding staining in serial paraffin sections, taking advantage of strong binding affinites of macrophage cells for peanut agglutinin after neuraminidase treatment and for ricinus communis agglutinin. Necrotic changes detected in three definite areas of the distal mesoderm of normal limb buds exhibit characteristic spatial temporal relationships with earlier cytolytic changes affecting the pre- and postaxial parts of the a.e.r. Two of them, known as the primary preaxial site (fpp) and the anterior marginal necrotic zone (AMNZ) appeared deeply modified in mutant embryos as compared to the posterior marginal necrotic zone (PMNZ) which remained unaffected. Macrophage cells loaded with cell debris appear in advance and in excessive number in the fpp of Os/+ limb buds. Conversely, they were found absent or locally reduced in number in the fpp and AMNZ of Xpl/+ limb buds which otherwise develop in the same area a preaxial protrusion covered with a healthy portion of the a.e.r. Hybrid Os/+/Xpl/+ limb buds expressing both mutant genes develop a smaller and macrophage-free preaxial protrusion which coexists with residual and locally excessive necrotic changes in its immediate surrounding and is covered with a normally necrotic portion of the a.e.r. Microscopic observations collected in the limb buds of all phenotypes, though more frequently in Os/+ limb buds, strongly suggest that in all three necrotic sites examined, macrophage cells of vascular origin somehow contribute to the clearance of ectodermal necrotic debris and eventually return in the blood stream through the marginal vein and its affluents.
This paper references
Ectodermal influence on physiological cell death in the posterior necrotic zone of the chick wing bud.
R. G. Brewton (1988)
The zone of polarizing activity: evidence for a role in normal chick limb morphogenesis.
D. Summerbell (1979)
The ectodermal cells of the limb bud: source of the mesodermal
Histochemical observations on the developing foot of normal, oligosyndactylous (Os-plus) and syndactylous (sm-sm) mouse embryos.
J. Milaire (1967)
Prevention of Genetic Polydactyly in Polydactyly Nagoya (Pdn) Mice in Vitro by Surgical Treatment of Foot Plate during Embryogenesis
I. Naruse (1986)
Ectodermal and mesodermal cell death patterns in 6-mercaptopurine riboside-induced digital deformities.
W. J. Scott (1980)
Aspirin-induced teratogenesis: a unique pattern of cell death and subsequent polydactyly in the rat.
K. Klein (1981)
Cell death in the posterior necrotic zone (PNZ) of the chick wing-bud: a stereoscan and ultrastructural survey of autolysis and cell fragmentation.
J. Hurlé (1978)
The effect of 5-bromodeo~ridine on mouse limb development
Pathogenesis ofbromodeokyuridine induced polydactyly
W. J. SCOTT. (1981)
Interdtgital tissue chondrogenesis induced by surgical removal of the ectoderm in the embryonic chick leg bud . 1
Y. GANAN. (1986)
The morphology of various types of cell death in prenatal tissues.
J. Schweichel (1973)
Hereditary and ir'lduced modifications of the r'lormal necrotic patterns In the developing limb buds of the rat and mouse: facts ar'ld hypotheses
J. MILA-IRE (1983)
Cell death in embryogenesis
J. Hinchliffe (1981)
The ectodermal cells of the limb bud : source of the mesodermal compartment
R. DE WATER (1991)
Control of maintenance and anteroposterior skeletal differentiation of the anterior mesenchyme of the chick wing bud by its posterior margin (the ZPA).
J. Hinchliffe (1981)
Development of X - linked polydactylyIn the mouse . I . Analysisof the malformations inembryos and full term fetuses
J. MILAIRE (1989)
Fine structure of the regressing interdigital membranes during the formation of the digits of the chick embryo leg bud.
J. Hurlé (1983)
Evolution des processus degeneratifs dans la cape apicale au cours du developpement des membres chez Ie rat et la souris
J. (1967)
Rudimentation digltale au cours du developpement normal de I ' autopode chez les mammiferes
J. MILA-IRE (1976)
Delayed appearance of ectodermal cell death as a mechanism of polydactyly induction
W. Scott (1977)
Interdigital tissue chondrogenesis induced by surgical removal of the ectoderm in the embryonic chick leg bud.
J. Hurlé (1986)
The zone of polarizing activity , evidence for a role m normal chick morphogeneSIS . 1
A. G. WATSON (1977)
Macrophage recognition of cells undergoing programmed cell death (apoptosis).
E. Duvall (1985)

This paper is referenced by
Insulin-like growth factor axis during embryonic development.
G. Allan (2001)
Altered Hox expression and increased cell death distinguish Hypodactyly from Hoxa13 null mice.
L. C. Post (1999)
Developmental basis of mammalian digit reduction: a case study in pigs
K. Sears (2011)
Influence of digits, ectoderm, and retinoic acid on chondrogenesis by mouse interdigital mesoderm in culture
Kenneth K. H. Lee (1994)
EGF , epithelium and Comparison of Hypodactyly and Hoxa 13 null mice 287 Altered Hox expression and increased cell death distinguish Hypodactyly from Hoxa 13 null mice
L. C. Post (1999)
Cellular analysis of limb development in the mouse mutant hypodactyly.
K. E. Robertson (1996)
Review of the Molecular Development of the Thumb: Digit Primera
K. Oberg (2014)
Ectopic expression of Msx-2 in posterior limb bud mesoderm impairs limb morphogenesis while inducing BMP-4 expression, inhibiting cell proliferation, and promoting apoptosis.
D. Ferrari (1998)
FGF-regulated Etv genes are essential for repressing Shh expression in mouse limb buds.
Z. Zhang (2009)
Altered expression of insulin-like growth factor -1 and insulin like growth factor binding proteins-2 and 5 in the mouse mutant Hypodactyly (Hd) correlates with sites of apoptotic activity
G. Allan (2000)
Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4.
N. R. Dunn (1997)
Developmental biology of the upper limb.
S. Guéro (2018)
Birth and death of cells in limb development: A mapping study
M. Fernandez-Teran (2006)
Chromosomal mapping of five highly conserved murine homologues of the Drosophila RING finger gene seven-in-absentia.
A. Holloway (1997)
Cell death in the developing vertebrate limb: A Locally regulated mechanism contributing to musculoskeletal tissue morphogenesis and differentiation.
J. Montero (2020)
Apoptosis is involved in the disappearance of the diastemal dental primordia in mouse embryo.
J. Tureckova (1996)
Gene and protein interactions in limb development : the case of Msx and Gli3
M. Shanmugasundaram (2015)
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