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Isolation, Characterisation And In Vitro Screening Of Anticataract Potential Of Fucoidan From Sargassum Wightii Greville

Sathiya Ramu, Shah Akshay Mahendra, Keerthana Kunchakuri, Pooja P. Karadi, K. Swetha, Gowri Radhakrishnan
Published 2018 · Biology

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Introduction: The present study was designed to isolate Fucoidan from Sargassum wightii Greville and to evaluate its in-vitro anti cataract potential against galactose induced cataract in isolated goat lenses.Methods: Fucoidan was isolated from S. wightii Greville and its sulfate content was estimated through barium chloride method. Isolated goat lenses were divided into 5 groups as group-I (normal control- vehicle treated), group-II (disease control- galactose treated 55 mM), group-III (galactose 55 mM + standard-ascorbic acid 20 μg/mL), group-IV (galactose 55 mM + Fucoidan-20 μg/mL) and group-V (galactose 55 mM + Fucoidan 40 μg/mL) and incubated for 72 hours, respectively, and subjected to biochemical estimations. The opacity of lenses was also noted prior to biochemical estimation.Results: The percentage yield of isolated Fucoidan was found to be 0.65%. The extent of sulfation in the isolated Fucoidan was found to be as high as 33%. In high-performance thin-layer chromatography (HPTLC) studies, Peak 3 with Rf value of 0.18 matched with the standard D-glucose Rf value. Galactose induced cataractous lenses showed significant oxidative stress when compared to normal lenses whereas treatment with Fucoidan 20 and 40 μg/mL significantly combated oxidative stress and prevented the development of cataract when compared to cataractous lenses. The results obtained with the treatment of Fucoidan were dose dependant and comparable to standard.Conclusion: The present study substantiates the claim of anti-cataract potential of Fucoidan, which may be correlated to its anti-oxidant property.
This paper references
10.1007/s10517-007-0226-4
Antitumor and antimetastatic activity of fucoidan, a sulfated polysaccharide isolated from the Okhotsk sea Fucus evanescens brown alga
T. V. Alekseyenko (2007)
10.3923/PJBS.2014.402.407
In vitro antioxidant properties of fucoidan fractions from Sargassum tenerrimum.
T. Marudhupandi (2014)
10.1155/2014/865375
Anticancer Effect of Fucoidan in Combination with Tyrosine Kinase Inhibitor Lapatinib
Byeongsang Oh (2014)
10.1016/J.
Características inmunológicas claves en la fisiopatología de la sepsis. Infectio
G. Faÿ (2009)
10.3923/IJP.2013.524.532
Inhibitory Effect of Sargassum patens on Inflammation and Melanogenesis
K. Kim (2013)
10.3390/md11051456
Structural Characteristics and Anticancer Activity of Fucoidan from the Brown Alga Sargassum mcclurei
Pham Duc Thinh (2013)
10.3390/md14080145
Fucoidans in Nanomedicine
L. Chollet (2016)
10.1016/0003-2697(72)90132-7
Colorimetric assay of catalase.
A. K. Sinha (1972)
10.15744/2575-5498.1.103
Antifungal Potential in Crude Extracts of Five Selected Brown Seaweeds Collected from the Western Libya Coast
A. Khallil (2016)
10.29333/ejgm/82712
Potential of Allylmercaptocaptopril as Anti Cataract Agent Against Galactosemic Cataract in Rats: An In Vitro And In Vivo Studies
Karunanidhi Santhana Lakshmi (2011)
10.1111/J.1749-7345.2012.00596.X
Antiviral Effect of Fucoidan Extracted from the Brown Seaweed, Sargassum wightii, on Shrimp Penaeus monodon Postlarvae against White Spot Syndrome Virus
M. Sivagnanavelmurugan (2012)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1093/GLYCOB/CWM014
A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds.
A. Cumashi (2007)
10.13005/BPJ/948
Evaluation of the Antioxidant, Hypoglycaemic and Anti-diabetic Activities of Some Seaweed Collected From the East Coast of India.
Lucy Mohapatra (2016)
10.3390/md12063292
The Protective Effect of Fucoidan in Rats with Streptozotocin-Induced Diabetic Nephropathy
Jing Wang (2014)
10.1039/C3RA23373A
Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation
M. T. Ale (2013)
10.1063/1.4966783
Effects of extraction solvent on fucose content in fucoidan extracted from brown seaweed (Sargassum sp.) from Pulau Langkawi, Kedah, Malaysia
Bibi Marliana Baba (2016)
10.1134/S1990750809010119
Anticoagulant activity of fucoidans from brown algae
N. A. Ushakova (2008)
10.3390/md12020851
Fucoidan as a Marine Anticancer Agent in Preclinical Development
J. Kwak (2014)
10.4014/JMB.1311.11025
Anti-inflammatory activity of ethanolic extract of Sargassum micracanthum.
Dahyun Jeong (2013)



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