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GAP-43 Expression In Primary Sensory Neurons Following Central Axotomy

M. S. Chong, M. Reynolds, N. Irwin, R. Coggeshall, P. Emson, L. Benowitz, C. Woolf
Published 1994 · Biology, Medicine

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Primary sensory neurons are capable of successful regenerative growth in response to peripheral nerve but not dorsal root injury. The present study is concerned with the differential expression of the mRNA for GAP- 43, a growth-associated protein, in these sensory neurons, in response to injury of their central or peripheral axonal branches. Peripheral axotomy resulted in an elevation in message detectable within 24 hr, using Northern blot and in situ hybridization, which was maintained for 30 d, whereas dorsal root section produced no change except a transient and small increase if the axotomy was immediately adjacent to the dorsal root ganglia (DRG). Dorsal root section had no effect on GAP-43 mRNA levels in the dorsal horn or in neighboring intact DRG. It also failed to alter the laminar boundaries of the GAP-43 central terminal labeling produced by peripheral nerve section, even though vacant synaptic sites were produced in unstained laminae by this procedure. This indicates that the location of GAP-43 immunolabeling in the central terminals of primed sensory cells may not depend only on the location of vacant synaptic sites. We conclude that distinct control mechanisms regulate the response of DRG neurons to peripheral nerve and dorsal root injury, and these may be related both to the glial environment and the particular target influences exerted on the central and peripheral branches of the primary sensory neuron. Central denervation alone is insufficient to upregulate GAP-43 levels, and this may explain the relative absence of collateral sprouting after the production of central vacant synaptic sites. The failure of dorsal root section to increase GAP-43 expression may contribute to the poor regenerative response initiated by such lesions.
This paper references
10.1002/CNE.902830410
Changes in dorsal horn synaptic disc numbers following unilateral dorsal rhizotomy
K. Chung (1989)
GAP-43 appears in the rat dorsal horn following peripheral nerve injury
WoolfCJ (1990)
Axonal transport and localization
W Tetzlaff (1989)
10.1111/j.1460-9568.1990.tb00440.x
B‐50/GAP43 Expression Correlates with Process Outgrowth in the Embryonic Mouse Nervous System
S. Biffo (1990)
10.1007/BF01795309
Nerve growth factor protects adult sensory neurons from cell death and atrophy caused by nerve injury
K. Rich (1987)
Dependence of GAP-43 (B50,Fl) transport on axonal regeneration in rat dorsal root ganglion neurons
MA Bisby (1988)
10.1038/355075A0
Peripheral nerve injury triggers central sprouting of myelinated afferents
C. Woolf (1992)
10.1126/SCIENCE.2658062
The neuronal growth-associated protein GAP-43 induces filopodia in non-neuronal cells.
M. X. Zuber (1989)
10.1016/0006-291X(92)91273-S
Inhibition of nerve growth factor-induced B-50/GAP-43 expression by antisense oligomers interferes with neurite outgrowth of PC12 cells.
E. R. Jap Tjoen San (1992)
10.1083/JCB.89.1.96
Axonally transported proteins associated with axon growth in rabbit central and peripheral nervous systems
J. Skene (1981)
10.1002/CNE.902750209
Lack of central sprouting of primary afferent fibers after ricin deafferentation
L. Pubols (1988)
10.1523/JNEUROSCI.08-01-00339.1988
Anatomical distribution of the growth-associated protein GAP-43/B-50 in the adult rat brain
L. Benowitz (1988)
10.1016/0006-8993(82)91059-9
Lack of sprouting and its presence after lesions of the cat spinal cord
M. Goldberger (1982)
Nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve
M Fitzgerald (1985)
10.1523/JNEUROSCI.06-06-01843.1986
A protein associated with axon growth, GAP-43, is widely distributed and developmentally regulated in rat CNS
R. Jacobson (1986)
10.1016/0169-328X(90)90071-K
Transfection of PC12 cells with the human GAP-43 gene: effects on neurite outgrowth and regeneration.
B. Yankner (1990)
10.1016/S0014-4886(05)80003-2
Myelin-associated inhibitors of neurite growth
M. Schwab (1990)
10.1002/CNE.902220402
A study of axonal diameters and areas in lumbosacral roots and nerves in the rat
Y. S. Suh (1984)
10.1016/0006-8993(92)91669-6
Differential expression of growth-associated protein (GAP-43) mRNA in rat primary sensory neurons after peripheral nerve lesion: a non-radioactive in situ hybridisation study
U. Wiese (1992)
regeneration: the ultrastructure of reactive astrocytes and abortive axon terminals in the transitional zone of the dorsal root
YS (1984)
10.1083/JCB.120.2.503
Phosphorylation-site mutagenesis of the growth-associated protein GAP- 43 modulates its effects on cell spreading and morphology
F. Widmer (1993)
10.1016/0306-4522(91)90209-7
GAP-43 expression in the developing rat lumbar spinal cord
M. Fitzgerald (1991)
A comparison of the regenerating potential of dorsal root fibers into grey or white matter of the adult rat spinal cord
JD Siegal (1990)
Regenerating axons form terminals at astrocytes
T Carlstedt (1985)
10.1126/science.3738509
A protein induced during nerve growth (GAP-43) is a major component of growth-cone membranes
J. Skene (1986)
10.1016/0169-328X(90)90004-W
The pattern of GAP-43 immunostaining changes in the rat hippocampal formation during reactive synaptogenesis.
L. Benowitz (1990)
10.1111/j.1699-0463.1988.tb00954.x
The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis
H. Gundersen (1988)
10.1523/JNEUROSCI.12-12-04783.1992
Axo-glial interactions at the dorsal root transitional zone regulate neurofilament protein synthesis in axotomized sensory neurons
F. Liuzzi (1992)
A comparison of the regenerating potential of dorsal root fibers into grey or white matter
JD Siegal (1990)
10.1016/0166-2236(90)90098-U
Myelin-associated inhibitors of neurite growth and regeneration in the CNS
M. Schwab (1990)
10.1038/309791A0
Peripheral injury enhances central regeneration of primary sensory neurones
P. Richardson (1984)
10.1016/0304-3940(91)90331-M
Distribution of the growth associated protein GAP-43 in the central processes of axotomized primary afferents in the adult rat spinal cord; presence of growth cone-like structures
R. Coggeshall (1991)
regeneration in the CNS
TB Shea (1991)
10.1523/JNEUROSCI.11-04-00943.1991
Regulation of axonal caliber, neurofilament content, and nuclear localization in mature sensory neurons by nerve growth factor
B. Gold (1991)
10.1016/0006-8993(85)90396-8
Nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section
M. Fitzgerald (1985)
10.1016/0169-328X(93)90005-A
Expression of mRNAs for neurotrophin receptors in the dorsal root ganglion and spinal cord during development and following peripheral or central axotomy.
P. Ernfors (1993)
Chronic peripheral nerve section results in a rearrangement of the central terminals of axotomized A beta primary afferent neurons in the rat spinal cord
P Shortland (1993)
Emson PC f 1992a) GAP-43 and lumbar dorsal roe; regeneration into long peripheral nerve grafts in rats
CJ AndersonPN.ChoneMS.Woolf (1992)
GAP - 43 and lumbar dorsal roe ; regeneration into long peripheral nerve grafts in rats
PN Anderson
10.1083/JCB.116.6.1455
GAP-43 is expressed by nonmyelin-forming Schwann cells of the peripheral nervous system
R. Curtis (1992)
10.1111/j.1460-9568.1992.tb00115.x
GAP‐43 mRNA in Rat Spinal Cord and Dorsal Root Ganglia Neurons: Developmental Changes and Re‐expression Following Peripheral Nerve Injury
M. S. Chong (1992)
10.1523/JNEUROSCI.10-03-00926.1990
Correlation between GAP43 and nerve growth factor receptors in rat sensory neurons
V. Verge (1990)
10.1073/PNAS.83.10.3537
Growth-associated protein, GAP-43, a polypeptide that is induced when neurons extend axons, is a component of growth cones and corresponds to pp46, a major polypeptide of a subcellular fraction enriched in growth cones.
K. Meiri (1986)
NGF does not regulate GAP 43 mRNA levels in adult rat dorsal root ganglion neurons
M Hu-Tsai (1992)
10.1126/SCIENCE.3603044
Astrocytes block axonal regeneration in mammals by activating the physiological stop pathway.
F. Liuzzi (1987)
10.1016/0169-328X(87)90012-X
The neuronal growth-associated protein GAP-43 (B-50, F1): neuronal specificity, developmental regulation and regional distribution of the human and rat mRNAs.
R. Neve (1987)
10.1016/0306-4522(91)90117-7
Time-dependent differences in the increase in GAP-43 expression in dorsal root ganglion cells after peripheral axotomy
T. Sommervaille (1991)
10.1007/BF01188402
Ultrastructural changes of the central scalloped (C1) primary afferent endings of synaptic glomeruli in the substantia gelatinosa Rolandi of the rat after peripheral neurotomy
J. Castro-Lopes (1990)
Partial Denervation of the medial gastrocnemius muscle results in GAP-43 immunoreactivity in sprouting axons and Schwann cells
CJ Mehta A. Revnolds ML. Woolf (1993)
Chronic peripheral nerve section results in a rearrangement of the central terminals of axotomized A beta
P Shortland (1993)
10.1016/0166-2236(92)90338-9
Intrinsic neuronal determinants of regeneration
J. Fawcett (1992)
10.1523/JNEUROSCI.09-03-00893.1989
Expression of GAP-43, a rapidly transported growth-associated protein, and class II beta tubulin, a slowly transported cytoskeletal protein, are coordinated in regenerating neurons
P. Hoffman (1989)
10.1523/JNEUROSCI.12-10-03999.1992
Denervation of the motor endplate results in the rapid expression by terminal Schwann cells of the growth-associated protein GAP-43
C. Woolf (1992)
10.1007/BF01237907
Electron microscopic immunocytochemistry of GAP-43 within proximal and chronically denervated distal stumps of transected peripheral nerve
S. Hall (1992)
10.1523/JNEUROSCI.03-02-00243.1983
Correlation of axonal regeneration and slow component B in two branches of a single axon
J. Wujek (1983)
10.1523/JNEUROSCI.11-12-03738.1991
Fate of GAP-43 in ascending spinal axons of DRG neurons after peripheral nerve injury: delayed accumulation and correlation with regenerative potential
D. Schreyer (1991)
10.1016/0166-2236(87)90135-4
A membrane phosphoprotein associated with neural development, axonal regeneration, phospholipid metabolism, and synaptic plasticity
L. Benowitz (1987)
10.1002/CNE.902150206
An examination of intraspinal sprouting in dorsal root axons with the tracer horseradish peroxidase
B. Rodin (1983)
Post-translational membrane attachment and dynamic fatty acylation of a neuronal growth cone protein, GAP
JHP Skene (1989)
10.1523/JNEUROSCI.06-11-03205.1986
Replacement of synaptic terminals in lamina II and Clarke's nucleus after unilateral lumbosacral dorsal rhizotomy in adult cats
M. Murray (1986)
10.1083/JCB.123.2.417
Depletion of 43-kD growth-associated protein in primary sensory neurons leads to diminished formation and spreading of growth cones
L. Aigner (1993)
10.1083/JCB.108.2.613
Posttranslational membrane attachment and dynamic fatty acylation of a neuronal growth cone protein, GAP-43
J. Skene (1989)
Co-localization of nerve growth factor receptor mRNA in primary sensory neurons: responses to injury, and infusion by nerve growth factors
VMK Verge (1993)
10.1016/0006-8993(87)91096-1
Axonal regeneration in dorsal spinal roots is accelerated by peripheral axonal transection
P. Richardson (1987)
10.1111/j.1471-4159.1993.tb05862.x
ADP‐Ribosylation of the Neuronal Phosphoprotein B‐50/GAP‐43
P. Coggins (1993)
10.1111/j.1471-4159.1991.tb11398.x
B‐50 (GAP‐43): Biochemistry and Functional Neurochemistry of a Neuron‐Specific Phosphoprotein
P. Coggins (1991)
10.1016/0169-328X(92)90024-6
Synthesis and transport of GAP-43 in entorhinal cortex neurons and perforant pathway during lesion-induced sprouting and reactive synaptogenesis.
L. H. Lin (1992)
10.1016/0006-8993(88)90509-4
Dependence of GAP43 (B50, F1) transport on axonal regeneration in rat dorsal root ganglion neurons
M. Bisby (1988)
10.1021/BI00591A005
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
J. Chirgwin (1979)
10.1002/JNR.490320112
Light and electron microscopic localization of B‐50 (GAP43) in the rat spinal cord during transganglionic degenerative atrophy and regeneration
E. Knyihár-csillik (1992)
10.1523/JNEUROSCI.10-07-02215.1990
A comparison of peripheral and central axotomy effects on neurofilament and tubulin gene expression in rat dorsal root ganglion neurons
J. Wong (1990)
Failure of regenerating dorsal root axons to regrow into the spinal cord
CS Perkins (1980)
10.1523/JNEUROSCI.11-08-02528.1991
Response of facial and rubrospinal neurons to axotomy: changes in mRNA expression for cytoskeletal proteins and GAP-43
W. Tetzlaff (1991)
10.1523/JNEUROSCI.11-04-00972.1991
Inflammation near the nerve cell body enhances axonal regeneration
X. Lu (1991)
10.1002/CNE.902880209
Deafferentation‐induced expansion of saphenous terminal field labelling in the adult rat dorsal horn following pronase injection of the sciatic nerve
C. Lamotte (1989)
10.1016/s0074-7742(08)60183-x
The axon reaction: a review of the principal features of perikaryal responses to axon injury.
A. Lieberman (1971)
Increased transport of 4400-4900 dalton acidic proteins during regeneration of the goldfish optic nerve: a 2-dimensional analysis
LI Benowitz (1983)
Sprouting of peripheral regenerating nrimarv sensorv neurones in the adult central nervous svstem
SB McMahon (1991)
The effects ofa sciatic nerve lesion on lumbar dorsal root regeneration into long peripheral nerve grafts in rats
PN Anderson (1992)
1) GAP-43 expression in-the developing rat lumbar spinal cord
M Fitzgerald
10.1523/JNEUROSCI.09-10-03505.1989
Expression of growth-associated protein B-50 (GAP43) in dorsal root ganglia and sciatic nerve during regenerative sprouting
C. E. van der Zee (1989)
1) Axonally transported proteins associated with axon growth in rabbit central and peripheral nervous system
Jhp Skene
10.1523/JNEUROSCI.11-06-01685.1991
Phospholipid-mediated delivery of anti-GAP-43 antibodies into neuroblastoma cells prevents neuritogenesis
T. Shea (1991)
10.1016/0166-2236(92)90339-A
A consideration of neural counting methods
R. Coggeshall (1992)
10.1016/0896-6273(91)90066-9
Expression of the growth-associated protein GAP-43 in adult rat retinal ganglion cells following axon injury
S. Doster (1991)
10.1523/JNEUROSCI.04-07-01736.1984
A conditioning lesion of the peripheral axons of dorsal root ganglion cells accelerates regeneration of only their peripheral axons
M. Oblinger (1984)
10.1523/JNEUROSCI.06-09-02563.1986
Elevated synthesis of an axonally transported protein correlates with axon outgrowth in normal and injured pyramidal tracts
K. Kalil (1986)
10.1016/0169-328X(87)90014-3
Axons of the pyramidal tract do not increase their transport of growth-associated proteins after axotomy.
T. Reh (1987)
10.1016/S0079-6123(08)61846-4
Inhibition of regeneration: the ultrastructure of reactive astrocytes and abortive axon terminals in the transition zone of the dorsal root.
L. Stensaas (1987)
The pattern of GAP
LI Benowitz (1990)
10.1126/SCIENCE.2437653
Cloning of complementary DNA for GAP-43, a neuronal growth-related protein.
L. Karns (1987)
10.1523/JNEUROSCI.09-04-01452.1989
Rapid induction of the major embryonic alpha-tubulin mRNA, T alpha 1, during nerve regeneration in adult rats
F. Miller (1989)
10.1523/JNEUROSCI.10-06-02018.1990
Influence of nerve growth factor on neurofilament gene expression in mature primary sensory neurons
V. Verge (1990)
10.1523/JNEUROSCI.08-05-01739.1988
Neurofilament protein synthesis in DRG neurons decreases more after peripheral axotomy than after central axotomy
S. Greenberg (1988)
10.1523/JNEUROSCI.09-04-01303.1989
Axonal transport and localization of B-50/GAP-43-like immunoreactivity in regenerating sciatic and facial nerves of the rat
W. Tetzlaff (1989)
10.1001/ARCHNEURPSYC.1958.02340010064005
Intraspinal sprouting of dorsal root axons; development of new collaterals and preterminals following partial denervation of the spinal cord in the cat.
C. N. Liu (1958)
10.1523/JNEUROSCI.11-02-00543.1991
NGF rescues substance P expression but not neurofilament or tubulin gene expression in axotomized sensory neurons
J. Wong (1991)
10.1146/ANNUREV.NE.12.030189.001015
Axonal growth-associated proteins.
J. Skene (1989)
10.1113/jphysiol.1977.sp011944
Membrane properties and conduction velocity in sensory neurones following central or peripheral axotomy
G. Czéh (1977)
10.1111/j.1471-4159.1985.tb08728.x
Resolution of Rat Brain Synaptic Phosphoprotein B‐50 into Multiple Forms by Two‐Dimensional Electrophoresis: Evidence for Multisite Phosphorylation
H. Zwiers (1985)



This paper is referenced by
10.1016/j.expneurol.2006.02.134
Local down-regulation of myelin-associated glycoprotein permits axonal sprouting with chronic nerve compression injury
R. Gupta (2006)
10.1517/14728220903307517
Molecular targets for axon regeneration: focus on the intrinsic pathways
S. D. Giovanni (2009)
10.1016/0092-8674(95)90168-X
Overexpression of the neural growth-associated protein GAP-43 induces nerve sprouting in the adult nervous system of transgenic mice
L. Aigner (1995)
10.1111/j.1460-9568.2006.05218.x
Riluzole promotes cell survival and neurite outgrowth in rat sensory neurones in vitro
P. Shortland (2006)
10.1002/cne.10850
Cell type‐ and region‐specific expression of protein kinase C‐substrate mRNAs in the cerebellum of the macaque monkey
N. Higo (2003)
10.1016/j.neuropharm.2017.10.025
Activation of ERK/CREB pathway in noradrenergic neurons contributes to hypernociceptive phenotype in H4 receptor knockout mice after nerve injury
M. D. Sanna (2018)
10.1167/IOVS.02-0779
Neurochemical correlates of cortical plasticity after unilateral elevated intraocular pressure in a primate model of glaucoma.
D. Lam (2003)
10.1002/(SICI)1096-9861(19990719)410:1<42::AID-CNE5>3.0.CO;2-F
Axonal regeneration from injured dorsal roots into the spinal cord of adult rats
M. S. Chong (1999)
10.1523/JNEUROSCI.15-06-04429.1995
Expression of molecules associated with neuronal plasticity in the striatum after aspiration and thermocoagulatory lesions of the cerebral cortex in adult rats
F. Szele (1995)
10.1038/82921
Turbocharging neurons for growth: accelerating regeneration in the adult CNS
C. Woolf (2001)
10.1523/JNEUROSCI.0903-17.2017
Postinjury Induction of Activated ErbB2 Selectively Hyperactivates Denervated Schwann Cells and Promotes Robust Dorsal Root Axon Regeneration
Seung Baek Han (2017)
10.1016/S0896-6273(00)80755-2
Regeneration of Dorsal Column Fibers into and beyond the Lesion Site following Adult Spinal Cord Injury
S. Neumann (1999)
10.1523/JNEUROSCI.18-15-05891.1998
Heat Shock Protein 27: Developmental Regulation and Expression after Peripheral Nerve Injury
M. Costigan (1998)
10.1016/0165-3806(95)00206-5
Development and activity-dependent expression of neuronal marker proteins in organotypic cultures of rat visual cortex.
G. Kierstein (1996)
10.1083/jcb.200703209
Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs
Dianna E Willis (2007)
10.1016/j.expneurol.2015.09.004
Exercise dependent increase in axon regeneration into peripheral nerve grafts by propriospinal but not sensory neurons after spinal cord injury is associated with modulation of regeneration-associated genes
R. Sachdeva (2016)
10.1016/j.neures.2006.07.014
Dynamic changes in Robo2 and Slit1 expression in adult rat dorsal root ganglion and sciatic nerve after peripheral and central axonal injury
Xinan Yi (2006)
10.1186/1471-2202-5-9
ATF3 upregulation in glia during Wallerian degeneration: differential expression in peripheral nerves and CNS white matter
D. Hunt (2003)
10.1002/BIES.950190906
Intrinsic neuronal determinants that promotes axonal sprouting and elongation
P. Caroni (1997)
10.1016/S0079-6123(08)62540-6
Chapter 13 Myelin-associated neurite growth inhibitors: regulators of plastic changes of neural connections in the central nervous system
J. Kapfhammer (1996)
10.1093/CERCOR/BHH073
Cell type- and region-specific expression of neurogranin mRNA in the cerebral cortex of the macaque monkey.
N. Higo (2004)
10.1101/446963
PP4-dependent HDAC3 dephosphorylation discriminates between axonal regeneration and regenerative failure
A. Hervera (2018)
10.1016/j.jneumeth.2014.01.020
Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
L. E. Savastano (2014)
10.1002/(SICI)1096-9861(19991129)414:4<495::AID-CNE6>3.0.CO;2-S
Influence of the axotomy to cell body distance in rat rubrospinal and spinal motoneurons: Differential regulation of GAP‐43, tubulins, and neurofilament‐M
K. J. Fernandes (1999)
10.1016/S0006-8993(99)01936-8
Increased expression of growth-associated protein (GAP-43) in lower urinary tract pathways following cyclophosphamide (CYP)-induced cystitis
M. Vizzard (1999)
10.1002/(SICI)1096-9861(19980209)391:2<259::AID-CNE8>3.0.CO;2-1
Differential effects of autologous peripheral nerve grafts to the corpus striatum of adult rats on the regeneration of axons of striatal and nigral neurons and on the expression of GAP‐43 and the cell adhesion molecules N‐CAM and L1
C.L. Woolhead (1998)
10.3389/fneur.2017.00049
Expression of Semaphorins, Neuropilins, VEGF, and Tenascins in Rat and Human Primary Sensory Neurons after a Dorsal Root Injury
Tomas Lindholm (2017)
10.14288/1.0091912
Galectin-1 in injury and regeneration
J. McGraw (2004)
10.1523/JNEUROSCI.19-10-03791.1999
Nature of the Retrograde Signal from Injured Nerves that Induces Interleukin-6 mRNA in Neurons
P. Murphy (1999)
10.1016/j.brainres.2006.01.062
Northern blot and in situ hybridization analyses for the neurogranin mRNA in the developing monkey cerebral cortex
N. Higo (2006)
10.1016/S0306-4522(00)00254-2
Axonal regeneration from CNS neurons in the cerebellum and brainstem of adult rats: correlation with the patterns of expression and distribution of messenger RNAs for L1, CHL1, c-jun and growth-associated protein-43
V. Chaisuksunt (2000)
10.15252/embj.2018101032
PP4‐dependent HDAC3 dephosphorylation discriminates between axonal regeneration and regenerative failure
A. Hervera (2019)
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