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The Density And Refractive Index Of Adsorbing Protein Layers.

János Vörös
Published 2004 · Chemistry, Medicine
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The structure of the adsorbing layers of native and denatured proteins (fibrinogen, gamma-immunoglobulin, albumin, and lysozyme) was studied on hydrophilic TiO(2) and hydrophobic Teflon-AF surfaces using the quartz crystal microbalance with dissipation and optical waveguide lightmode spectroscopy techniques. The density and the refractive index of the adsorbing protein layers could be determined from the complementary information provided by the two in situ instruments. The observed density and refractive index changes during the protein-adsorption process indicated the presence of conformational changes (e.g., partial unfolding) in general, especially upon contact with the hydrophobic surface. The structure of the formed layers was found to depend on the size of the proteins and on the experimental conditions. On the TiO(2) surface smaller proteins formed a denser layer than larger ones and the layer of unfolded proteins was less dense than that adsorbed from the native conformation. The hydrophobic surface induced denaturation and resulted in the formation of thin compact protein films of albumin and lysozyme. A linear correlation was found between the quartz crystal microbalance measured dissipation factor and the total water content of the layer, suggesting the existence of a dissipative process that is related to the solvent molecules present inside the adsorbed protein layer. Our measurements indicated that water and solvent molecules not only influence the 3D structure of proteins in solution but also play a crucial role in their adsorption onto surfaces.
This paper references
10.1016/S0956-5663(99)00040-8
Commercial quartz crystal microbalances : theory and applications
Ciara K O'Sullivan (1999)
10.1002/bip.1978.360170711
Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air–water interface
J. A. De Feijter (1978)
10.1016/S0167-5729(01)00020-6
The interaction of water with solid surfaces: fundamental aspects revisited
Michael Henderson (2002)
10.1364/AO.21.002960
Deposition and properties of optical oxide coatings from polymerized solutions.
Bulent E. Yoldas (1982)
10.1021/la00078a044
Thermal denaturation of an adsorbed fibrinogen layer studied by reflectometry
Pierre Schaaf (1987)
10.1007/s003400100501
Effect of patterns and inhomogeneities on the surface of waveguides used for optical waveguide lightmode spectroscopy applications
R. Horvath (2001)
10.1006/jcis.2001.8060
Protein adsorption on supported phospholipid bilayers.
Karin Glasmästar (2002)
10.1021/la0263920
Intact vesicle adsorption and supported biomembrane formation from vesicles in solution: Influence of surface chemistry, vesicle size, temperature, and osmotic pressure
Erik Reimhult (2003)
10.1016/S0001-8686(97)00040-7
Structure and reactivity of water at biomaterial surfaces.
Erwin A. Vogler (1998)
10.1016/S0956-5663(02)00050-7
Missing mass effect in biosensor's QCM applications.
Marina V. Voinova (2002)
10.1006/jcis.1998.5680
The Denaturation of Lysozyme Layers Adsorbed at the Hydrophobic Solid/Liquid Surface Studied by Neutron Reflection.
Lu. (1998)
10.1080/00032719908542912
Kinetic Studies of Affinity Interactions: Comparison of Piezoelectric and Resonant Mirror-Based Biosensors
Petr Skládal (1999)
10.1006/jcis.2002.8482
A comparative study of surfactant adsorption on model surfaces using the quartz crystal microbalance and the ellipsometer.
Johan J. R. Stålgren (2002)
10.1163/156856299X00540
Phosphorylcholine-containing polyurethanes for the control of protein adsorption and cell attachment via photoimmobilized laminin oligopeptides.
L. Ruiz (1999)
10.1023/A:1009074228662
Influence of polymer surface chemistry on frictional properties under protein-lubrication conditions: implications for hip-implant design
Martin Widmer (2001)
10.1021/la00078a045
Reflectometry as a technique to study the adsorption of human fibrinogen at the silica/solution interface
Pierre Schaaf (1987)
10.1002/jmr.544
Current and emerging commercial optical biosensors.
Cheryl L. Baird (2001)
10.1016/S0927-7765(02)00186-8
Adsorption of a small protein to a methyl-terminated hydrophobic surfaces: effect of protein-folding thermodynamics and kinetics
Daniel Erik Otzen (2003)
Ellipsometry on thin organic layers of biological interest: characterization and applications
U HansArwin (2000)
10.1021/la0112716
Using surface plasmon resonance and the quartz crystal microbalance to monitor in situ the interfacial behavior of thin organic films
Larry E. Bailey (2002)
10.1016/S0956-5663(00)00125-1
Comparison of surface plasmon resonance and quartz crystal microbalance in the study of whole blood and plasma coagulation.
T. Vikinge (2000)
10.1021/la971228b
Quantitative Interpretation of the Response of Surface Plasmon Resonance Sensors to Adsorbed Films
Linda S. Jung (1998)
10.1016/0956-5663(91)80006-J
Principles and sensitivities of integrated optical and surface plasmon sensors for direct affinity sensing and immunosensing
W. Lukosz (1991)
10.1006/jcis.2002.8247
The determination of thickness and surface mass density of mesothick immunoprecipitate layers by null ellipsometry and protein 125iodine labeling.
Johan Benesch (2002)
10.1002/jmr.598
Survey of the year 2001 commercial optical biosensor literature.
Rebecca L. Rich (2002)
10.1021/ac0106501
Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking: a quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study.
Fredrik Höök (2001)
10.1002/sia.995
Bioengineered material surfaces for medical applications
Hans Jörg Mathieu (2001)
10.1002/bip.1057
Static and dynamic light scattering approach to the hydration of hemoglobin and its supertetramers in the presence of osmolites.
Daniele Arosio (2002)
10.1063/1.1148069
Instrumental improvements in optical waveguide light mode spectroscopy for the study of biomolecule adsorption
R. Kurrat (1997)
10.1021/la961097z
Effect of surface properties and added electrolyte on the structure of β-casein layers adsorbed at the solid/aqueous interface
Tomas P J Kull (1997)
10.1063/1.1315338
Simultaneous determination of optical and acoustic thicknesses of protein layers using surface plasmon resonance spectroscopy and quartz crystal microweighing
Alexander Laschitsch (2000)
10.1021/ac960198z
Development of a surface acoustic wave immunosensor.
Waltraud Weisch (1996)
10.1016/S0142-9612(02)00103-5
Optical grating coupler biosensors.
J. Vörös (2002)
10.1021/la001746d
Evaluating Optical Techniques for Determining Film Structure: Optical Invariants for Anisotropic Dielectric Thin Films
Elizabeth K. Mann (2001)
10.1080/10409230290771456
The Hydrophobic Effect: A New Insight from Cold Denaturation and a Two-State Water Structure
C J Tsai (2002)
10.1063/1.1375156
Determination of structural parameters characterizing thin films by optical methods: A comparison between scanning angle reflectometry and optical waveguide lightmode spectroscopy
Catherine Picart (2001)
10.1007/BF01052813
Review of new experimental techniques for investigating random sequential adsorption
Jeremy J. Ramsden (1993)
10.1016/S0927-7765(01)00236-3
A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation
Fredrik Höök (2002)
10.1364/JOSAB.6.000209
Sensitivity of grating couplers as integrated-optical chemical sensors
Kurt Tiefenthaler (1989)
10.1016/S0927-7757(99)00409-4
From car parking to protein adsorption: an overview of sequential adsorption processes
Julian Talbot (1999)
10.1006/jmbi.1999.2829
The packing density in proteins: standard radii and volumes.
Jenny Hsin-Chun Tsai (1999)
10.1002/(SICI)1097-0282(199812)46:7<489::AID-BIP6>3.0.CO;2-E
Buffer dependence of refractive index increments of protein solutions
Vincent Ball (1998)



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Disk-based one-dimensional photonic crystal slabs for label-free immunosensing.
Gabriel Sancho-Fornes (2019)
10.1016/j.bios.2013.01.045
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Andreea Olaru (2013)
10.1109/JSEN.2016.2600632
Label-Free Immunosensor Based on Optical Fiber Fabry–Perot Interferometer
Xiaohui Liu (2016)
10.1016/J.TRIBOINT.2015.01.005
Effect of bovine serum albumin aqueous solution on steel slider surface under hydrodynamic lubrication
Xinming Li (2016)
10.1364/JOSAB.32.001788
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Tong Lin (2015)
10.1529/biophysj.107.104554
Chlorophyll ring deformation modulates Qy electronic energy in chlorophyll-protein complexes and generates spectral forms.
Giuseppe Zucchelli (2007)
10.1039/c7an00787f
Charged poly(N-isopropylacrylamide) nanogels for use as differential protein receptors in a turbidimetric sensor array.
Heidi R Culver (2017)
10.1016/j.biomaterials.2014.04.012
Effect of the interplay between protein and surface on the properties of adsorbed protein layers
Myriam M. Ouberai (2014)
10.1016/J.CES.2009.05.027
Proteolytic cleaning of a surface-bound rubisco protein stain
Sagheer A. Onaizi (2009)
10.1021/bm200070k
pH-Amplified multilayer films based on hyaluronan: influence of HA molecular weight and concentration on film growth and stability.
Liyan Shen (2011)
10.1021/bm201791p
Amphiphilic polybetaines: the effect of side-chain hydrophobicity on protein adsorption.
Semra Çolak (2012)
10.1109/jsen.2020.2981897
A Surface Plasmon Resonance Bio-Sensor based on Dual Core D-Shaped Photonic Crystal Fibre Embedded with Silver Nanowires for Multi-Sensing
Suoda Chu (2020)
10.1002/jmr.862
Survey of the year 2006 commercial optical biosensor literature.
Rebecca L. Rich (2007)
10.1021/acs.analchem.5b04814
Critical View on Electrochemical Impedance Spectroscopy Using the Ferri/Ferrocyanide Redox Couple at Gold Electrodes.
Stephan Vogt (2016)
10.1007/978-3-319-28926-7_23
Comparative studies on optical biosensors for detection of bio-Toxins
Aleksey Nabok (2016)
10.1364/OE.23.009192
On the performance of label-free biosensors based on vertical one-dimensional photonic crystal resonant cavities.
Salvatore Surdo (2015)
10.1021/es3022478
Adsorption of insecticidal Cry1Ab protein to humic substances. 1. Experimental approach and mechanistic aspects.
Michael Sander (2012)
10.1016/j.jcis.2011.09.068
Adsorption and protein-induced metal release from chromium metal and stainless steel.
Maria Lundin (2012)
10.1016/j.biomaterials.2011.05.060
Directed cell attachment by tropoelastin on masked plasma immersion ion implantation treated PTFE.
Daniel V. Bax (2011)
10.1016/j.jcis.2011.09.078
Humidity adsorption kinetics of a trypsin gel film.
Salih Okur (2012)
10.3929/ETHZ-A-007178205
Poly(alkyl-phosphonates), a modular approach to functionalization of surfaces
Christoph Mayer (2011)
10.1109/PGC.2010.5706106
Ultra-compact SOI micro rings for sensing applications
Sathish Malathi (2010)
10.1016/J.APSUSC.2012.05.020
Detailed evaluation of protein adsorption on alumina surface through surface plasmon resonance phenomenon
Hiroki Akasaka (2012)
10.1021/am3008517
Structural and functional characteristics of chimeric avidins physically adsorbed onto functionalized polythiophene thin films.
Willem M. Albers (2012)
10.1021/nn202013v
Template-stripped smooth Ag nanohole arrays with silica shells for surface plasmon resonance biosensing.
Hyungsoon Im (2011)
10.1088/2040-8978/13/7/075701
A reverse symmetry optical waveguide sensor using a plasma substrate
Sofyan A. Taya (2011)
10.1016/j.bios.2018.01.061
Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.
Wenchao Zhou (2018)
SEMICONDUCTOR AND INTEGRATED OPTOELECTRONICS ( SIOE ) Optimisation of distributed feedback laser biosensors
Julie Coote (2007)
Antibody Screening Using a Biophotonic Array Sensor for Immune System Response Profile
Thomas T. Read (2013)
10.1117/1.2837450
Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles.
Adam C. Curry (2008)
10.1007/S11082-014-0104-Z
Sensitivity estimation of metamaterial loaded planar waveguide
Anurag Upadhyay (2015)
10.1021/nn502992x
Ion-Specific Control of the Self-Assembly Dynamics of a Nanostructured Protein Lattice
Behzad Rad (2015)
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