The Density And Refractive Index Of Adsorbing Protein Layers.
Published 2004 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
The structure of the adsorbing layers of native and denatured proteins (fibrinogen, gamma-immunoglobulin, albumin, and lysozyme) was studied on hydrophilic TiO(2) and hydrophobic Teflon-AF surfaces using the quartz crystal microbalance with dissipation and optical waveguide lightmode spectroscopy techniques. The density and the refractive index of the adsorbing protein layers could be determined from the complementary information provided by the two in situ instruments. The observed density and refractive index changes during the protein-adsorption process indicated the presence of conformational changes (e.g., partial unfolding) in general, especially upon contact with the hydrophobic surface. The structure of the formed layers was found to depend on the size of the proteins and on the experimental conditions. On the TiO(2) surface smaller proteins formed a denser layer than larger ones and the layer of unfolded proteins was less dense than that adsorbed from the native conformation. The hydrophobic surface induced denaturation and resulted in the formation of thin compact protein films of albumin and lysozyme. A linear correlation was found between the quartz crystal microbalance measured dissipation factor and the total water content of the layer, suggesting the existence of a dissipative process that is related to the solvent molecules present inside the adsorbed protein layer. Our measurements indicated that water and solvent molecules not only influence the 3D structure of proteins in solution but also play a crucial role in their adsorption onto surfaces.
This paper references
Commercial quartz crystal microbalances : theory and applications
Ciara K O'Sullivan (1999)
Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air–water interface
J. A. De Feijter (1978)
The interaction of water with solid surfaces: fundamental aspects revisited
Michael Henderson (2002)
Deposition and properties of optical oxide coatings from polymerized solutions.
Bulent E. Yoldas (1982)
Thermal denaturation of an adsorbed fibrinogen layer studied by reflectometry
Pierre Schaaf (1987)
Effect of patterns and inhomogeneities on the surface of waveguides used for optical waveguide lightmode spectroscopy applications
R. Horvath (2001)
Protein adsorption on supported phospholipid bilayers.
Karin Glasmästar (2002)
Intact vesicle adsorption and supported biomembrane formation from vesicles in solution: Influence of surface chemistry, vesicle size, temperature, and osmotic pressure
Erik Reimhult (2003)
Structure and reactivity of water at biomaterial surfaces.
Erwin A. Vogler (1998)
Missing mass effect in biosensor's QCM applications.
Marina V. Voinova (2002)
The Denaturation of Lysozyme Layers Adsorbed at the Hydrophobic Solid/Liquid Surface Studied by Neutron Reflection.
Kinetic Studies of Affinity Interactions: Comparison of Piezoelectric and Resonant Mirror-Based Biosensors
Petr Skládal (1999)
A comparative study of surfactant adsorption on model surfaces using the quartz crystal microbalance and the ellipsometer.
Johan J. R. Stålgren (2002)
Phosphorylcholine-containing polyurethanes for the control of protein adsorption and cell attachment via photoimmobilized laminin oligopeptides.
L. Ruiz (1999)
Influence of polymer surface chemistry on frictional properties under protein-lubrication conditions: implications for hip-implant design
Martin Widmer (2001)
Reflectometry as a technique to study the adsorption of human fibrinogen at the silica/solution interface
Pierre Schaaf (1987)
Current and emerging commercial optical biosensors.
Cheryl L. Baird (2001)
Adsorption of a small protein to a methyl-terminated hydrophobic surfaces: effect of protein-folding thermodynamics and kinetics
Daniel Erik Otzen (2003)
Ellipsometry on thin organic layers of biological interest: characterization and applications
U HansArwin (2000)
Using surface plasmon resonance and the quartz crystal microbalance to monitor in situ the interfacial behavior of thin organic films
Larry E. Bailey (2002)
Comparison of surface plasmon resonance and quartz crystal microbalance in the study of whole blood and plasma coagulation.
T. Vikinge (2000)
Quantitative Interpretation of the Response of Surface Plasmon Resonance Sensors to Adsorbed Films
Linda S. Jung (1998)
Principles and sensitivities of integrated optical and surface plasmon sensors for direct affinity sensing and immunosensing
W. Lukosz (1991)
The determination of thickness and surface mass density of mesothick immunoprecipitate layers by null ellipsometry and protein 125iodine labeling.
Johan Benesch (2002)
Survey of the year 2001 commercial optical biosensor literature.
Rebecca L. Rich (2002)
Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking: a quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study.
Fredrik Höök (2001)
Bioengineered material surfaces for medical applications
Hans Jörg Mathieu (2001)
Static and dynamic light scattering approach to the hydration of hemoglobin and its supertetramers in the presence of osmolites.
Daniele Arosio (2002)
Instrumental improvements in optical waveguide light mode spectroscopy for the study of biomolecule adsorption
R. Kurrat (1997)
Effect of surface properties and added electrolyte on the structure of β-casein layers adsorbed at the solid/aqueous interface
Tomas P J Kull (1997)
Simultaneous determination of optical and acoustic thicknesses of protein layers using surface plasmon resonance spectroscopy and quartz crystal microweighing
Alexander Laschitsch (2000)
Development of a surface acoustic wave immunosensor.
Waltraud Weisch (1996)
Optical grating coupler biosensors.
J. Vörös (2002)
Evaluating Optical Techniques for Determining Film Structure: Optical Invariants for Anisotropic Dielectric Thin Films
Elizabeth K. Mann (2001)
The Hydrophobic Effect: A New Insight from Cold Denaturation and a Two-State Water Structure
C J Tsai (2002)
Determination of structural parameters characterizing thin films by optical methods: A comparison between scanning angle reflectometry and optical waveguide lightmode spectroscopy
Catherine Picart (2001)
Review of new experimental techniques for investigating random sequential adsorption
Jeremy J. Ramsden (1993)
A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation
Fredrik Höök (2002)
Sensitivity of grating couplers as integrated-optical chemical sensors
Kurt Tiefenthaler (1989)
From car parking to protein adsorption: an overview of sequential adsorption processes
Julian Talbot (1999)
The packing density in proteins: standard radii and volumes.
Jenny Hsin-Chun Tsai (1999)
Buffer dependence of refractive index increments of protein solutions
Vincent Ball (1998)
This paper is referenced by
Disk-based one-dimensional photonic crystal slabs for label-free immunosensing.
Gabriel Sancho-Fornes (2019)
Quality assessment of SPR sensor chips; case study on L1 chips.
Andreea Olaru (2013)
Label-Free Immunosensor Based on Optical Fiber Fabry–Perot Interferometer
Xiaohui Liu (2016)
Effect of bovine serum albumin aqueous solution on steel slider surface under hydrodynamic lubrication
Xinming Li (2016)
Design of an ultra-compact slotted photonic crystal nanobeam cavity for biosensing
Tong Lin (2015)
Chlorophyll ring deformation modulates Qy electronic energy in chlorophyll-protein complexes and generates spectral forms.
Giuseppe Zucchelli (2007)
Charged poly(N-isopropylacrylamide) nanogels for use as differential protein receptors in a turbidimetric sensor array.
Heidi R Culver (2017)
Effect of the interplay between protein and surface on the properties of adsorbed protein layers
Myriam M. Ouberai (2014)
Proteolytic cleaning of a surface-bound rubisco protein stain
Sagheer A. Onaizi (2009)
pH-Amplified multilayer films based on hyaluronan: influence of HA molecular weight and concentration on film growth and stability.
Liyan Shen (2011)
Amphiphilic polybetaines: the effect of side-chain hydrophobicity on protein adsorption.
Semra Çolak (2012)
A Surface Plasmon Resonance Bio-Sensor based on Dual Core D-Shaped Photonic Crystal Fibre Embedded with Silver Nanowires for Multi-Sensing
Suoda Chu (2020)
Survey of the year 2006 commercial optical biosensor literature.
Rebecca L. Rich (2007)
Critical View on Electrochemical Impedance Spectroscopy Using the Ferri/Ferrocyanide Redox Couple at Gold Electrodes.
Stephan Vogt (2016)
Comparative studies on optical biosensors for detection of bio-Toxins
Aleksey Nabok (2016)
On the performance of label-free biosensors based on vertical one-dimensional photonic crystal resonant cavities.
Salvatore Surdo (2015)
Adsorption of insecticidal Cry1Ab protein to humic substances. 1. Experimental approach and mechanistic aspects.
Michael Sander (2012)
Adsorption and protein-induced metal release from chromium metal and stainless steel.
Maria Lundin (2012)
Directed cell attachment by tropoelastin on masked plasma immersion ion implantation treated PTFE.
Daniel V. Bax (2011)
Humidity adsorption kinetics of a trypsin gel film.
Salih Okur (2012)
Poly(alkyl-phosphonates), a modular approach to functionalization of surfaces
Christoph Mayer (2011)
Ultra-compact SOI micro rings for sensing applications
Sathish Malathi (2010)
Detailed evaluation of protein adsorption on alumina surface through surface plasmon resonance phenomenon
Hiroki Akasaka (2012)
Structural and functional characteristics of chimeric avidins physically adsorbed onto functionalized polythiophene thin films.
Willem M. Albers (2012)
Template-stripped smooth Ag nanohole arrays with silica shells for surface plasmon resonance biosensing.
Hyungsoon Im (2011)
A reverse symmetry optical waveguide sensor using a plasma substrate
Sofyan A. Taya (2011)
Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.
Wenchao Zhou (2018)
SEMICONDUCTOR AND INTEGRATED OPTOELECTRONICS ( SIOE ) Optimisation of distributed feedback laser biosensors
Julie Coote (2007)
Antibody Screening Using a Biophotonic Array Sensor for Immune System Response Profile
Thomas T. Read (2013)
Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles.
Adam C. Curry (2008)
Sensitivity estimation of metamaterial loaded planar waveguide
Anurag Upadhyay (2015)
Ion-Specific Control of the Self-Assembly Dynamics of a Nanostructured Protein Lattice
Behzad Rad (2015)See more