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Methods For Quantifying Follicular Numbers Within The Mouse Ovary.
M. Myers, K. Britt, N. Wreford, F. Ebling, J. B. Kerr
Published 2004 · Biology, Medicine
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Accurate estimation of the number of ovarian follicles at various stages of development is an important indicator of the process of folliculogenesis in relation to the endocrine signals and paracrine/autocrine mechanisms that control the growth and maturation of the oocytes and their supporting follicular cells. There are 10-fold or greater differences in follicular numbers per ovary at similar ages and/or strains reported in earlier studies using various methods, leading to difficulties with interpretation of ovarian function in control vs experimental conditions. This study describes unbiased, assumption-free stereological methods for quantification of early and growing follicular numbers in the mouse ovary. A fractionator approach was used to sample a defined fraction of histological sections of adult wild-type ovaries. Primordial and primary follicles were counted independently with the optical and physical disector methods. The fractionator/disector methods, which are independent of follicular size or shape, gave estimations of 1930 +/- 286 (S.E.M.) and 2227 +/- 101 primordial follicles, and 137 +/- 25 and 265 +/- 32 primary follicles per ovary at 70 and 100 days of age respectively. From exact counts on serial sections, secondary and later follicular numbers at 100 days of age were estimated at 135 per ovary. Remnants of zona pellucidae (a marker of previous follicular atresia) were estimated using a fractionator/physical disector approach and were approximately 500 per ovary. The application of the quantitative methods described will facilitate an improved understanding of follicular dynamics and the factors that mediate their growth and maturation and allow for a better comparison between different studies.
This paper references
Control of Follicular Growth and Ovulation
B. Donovan (1967)
Dynamics of Ovarian Development in the FORKO Immature Mouse: Structural and Functional Implications for Ovarian Reserve1
Agneta Balla (2003)
Postnatal ovarian follicle development in hypogonadal (hpg) and normal mice and associated changes in the hypothalamic-pituitary ovarian axis.
D. Halpin (1986)
Follicle growth in the immature mouse ovary.
T. Pedersen (1969)
The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis
H. Gundersen (1988)
Suppression of Ovarian Follicle Activation in Mice by the Transcription Factor Foxo3a
D. Castrillon (2003)
Effect of Bcl-2 on the Primordial Follicle Endowment in the Mouse Ovary1
J. Flaws (2001)
Ovarian follicle counts – not as simple as 1, 2, 3
J. Tilly (2003)
An analytical model for ovarian follicle dynamics.
M. Faddy (1976)
An age-related ovarian phenotype in mice with targeted disruption of the Cyp 19 (aromatase) gene.
K. Britt (2000)
Tissue shrinkage and unbiased stereological estimation of particle number and size *
K. A. Dorph-Petersen (2001)
Ovarian follicle dynamics in mice: a comparative study of three inbred strains and an F1 hybrid.
M. Faddy (1983)
Characterization of oocyte and follicle development in growth differentiation factor-9-deficient mice.
M. J. Carabatsos (1998)
Molecular characterization of the follicle defects in the growth differentiation factor 9-deficient ovary.
J. Elvin (1999)
Anti-Müllerian Hormone Attenuates the Effects of FSH on Follicle Development in the Mouse Ovary.
A. Durlinger (2001)
Analyzing renal glomeruli with the new stereology.
J. F. Bertram (1995)
Untersuchungen uber den Bau der menschlichen hypophse mit besondener Berucksichtigung der quantitativen mikromorphologischen Verhaltnisse
S Floderus (1944)
Developmental and Molecular Aberrations Associated with Deterioration of Oogenesis During Complete or Partial Follicle-Stimulating Hormone Receptor Deficiency in Mice1
Y. Yang (2003)
Stereology of arbitrary particles *
H. Gundersen (1986)
Continuous low-dose treatment with brain-derived neurotrophic factor or neurotrophin-3 protects striatal medium spiny neurons from mild neonatal hypoxia/ischemia: a stereological study
K. A. Galvin (2003)
Hypophysectomy of the cyclic mouse. I. Effects on folliculogenesis, oocyte growth, and follicle-stimulating hormone and human chorionic gonadotropin receptors.
X. Wang (1993)
The early stages of follicular development: activation of primordial follicles and growth of preantral follicles.
J. Fortune (2003)
THE RELATIONSHIPS BETWEEN AGE, NUMBERS OF OOCYTES AND FERTILITY IN VIRGIN AND MULTIPAROUS MICE
E. C. Jones (1961)
Ablation of bcl-2 gene expression decreases the numbers of oocytes and primordial follicles established in the post-natal female mouse gonad.
V. Ratts (1995)
Evidence for genetic modifiers of ovarian follicular endowment and development from studies of five inbred mouse strains.
Jacqueline Canning (2003)
Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility
T. R. Kumar (1997)
An accurate, simple method for unbiased determination of primordial follicle number in the primate ovary.
P. Miller (1997)
Application of the Cavalieri principle and vertical sections method to lung: estimation of volume and pleural surface area
R. Michel (1988)
Physiological role of the aryl hydrocarbon receptor in mouse ovary development.
J. C. Benedict (2000)
Unbiased stereological quantification of neurons in the human spiral ganglion
A. Ishiyama (2001)
Initial and cyclic recruitment of ovarian follicles.
E. Mcgee (2000)
Comparison of random and serial sections in assessment of ovarian toxicity.
B. J. Smith (1991)
Impact of congenital or experimental hypogonadotrophism on the radiation sensitivity of the mouse ovary.
R. Gosden (1997)
A New Approach to an Unbiased Estimate of the Hepatic Stellate Cell Index in the Rat Liver: An Example in Healthy Conditions1
R. Marcos (2003)
The kinetics of pre‐antral follicle development in ovaries of CBA/Ca mice during the first 14 weeks of life
M. Faddy (1987)
Estrogen receptor null mice: what have we learned and where will they lead us?
J. F. Couse (1999)
Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator
M. West (1991)
The development of the mouse ovary from birth to maturity.
H. Peters (1969)
Experimental Models of Partial Lesion of Rat Spinal Cord to Investigate Neurodegeneration, Glial Activation, and Behavior Impairments
G. Chadi (2001)
Perspective: the ovarian follicle--a perspective in 2001.
J. Richards (2001)
Male reproductive phenotypes in double mutant mice lacking both FSHbeta and activin receptor IIA.
T. Kumar (2001)
Oocyte number in newborn mice after prenatal octylphenol exposure.
K. Sonne-Hansen (2003)
Targeted disruption of luteinizing hormone/human chorionic gonadotropin receptor gene.
Z. Lei (2001)
Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice
S. Inoue (2000)
Control of primordial follicle recruitment by anti-Müllerian hormone in the mouse ovary.
A. Durlinger (1999)
Male Reproductive Phenotypes in Double Mutant Mice Lacking Both FSHβ and Activin Receptor IIA.
T. Kumar (2001)
Bcl-x and Bax regulate mouse primordial germ cell survival and apoptosis during embryogenesis.
E. Rucker (2000)
The total number of neurons in the human neocortex unbiasedly estimated using optical disectors
H. Braendgaard (1990)
Smad 3 May Regulate Follicular Growth in the Mouse Ovary1
D. Tomić (2002)
Theory and practice of stereological techniques applied to the estimation of cell number and nuclear volume in the testis
N. Wreford (1995)
Software for counting cells and estimating structural volumes with the optical disector and fractionator
R. Bolender (1993)
Counting in the kidney.
J. Bertram (2001)
The unbiased estimation of number and sizes of arbitrary particles using the disector
D. Sterio (1984)
Haploinsufficiency of the Follicle-Stimulating Hormone Receptor Accelerates Oocyte Loss Inducing Early Reproductive Senescence and Biological Aging in Mice1
N. Danilovich (2002)
Effect of single and compound knockouts of estrogen receptors alpha (ERalpha) and beta (ERbeta) on mouse reproductive phenotypes.
S. Dupont (2000)
Structure-function correlation in the human placenta: the morphometric diffusing capacity for oxygen at full term.
T. Mayhew (1984)
Proposal for a classification of oocytes and follicles in the mouse ovary.
T. Pedersen (1968)
Estimation of nuclear population from microtome sections
M. Abercrombie (1946)
The efficiency of systematic sampling in stereology and its prediction *
H. Gundersen (1987)
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J. Stanley (2014)
Cisplatin-induced primordial follicle oocyte killing and loss of fertility are not prevented by imatinib
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J. Kim (2012)
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Oestrogenic action of neonatal tamoxifen on the hypothalamus and reproductive system in female mice.
R. Parandin (2016)
Influence of follicle stage on artificial ovary outcome using fibrin as a matrix.
M. C. Chiti (2016)
Surgical translocation and ultrasound bio-microscopy of the ovaries in rabbits.
M. Cervantes (2013)
Prepubertal bisphenol A exposure interferes with ovarian follicle development and its relevant gene expression.
Y. Li (2014)
Moderate episodic prenatal alcohol does not impact female offspring fertility in rats
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J. Wang (2021)
Beneficial Effect of Hyperbaric Oxygen Therapy on the Follicular Survival in the Mouse Ovary after Transplantation
S. Haya (2012)
Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice
Yanjun Yang (2019)
Study the effects of Corynebacterium pseudotuberculosis antigens in the improvement of fertility in female mice against the virulent pathogen with virulent type of same pathogen
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Análise morfológica e morfometrica de útero, ovário, glândulas Adrenais e perfil lipídico de ratas suplementadas com capsiate
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Umbilical Cord Mesenchymal Stem Cell Transplantation Prevents Chemotherapy-Induced Ovarian Failure via the NGF/TrkA Pathway in Rats
Q. Zheng (2019)
A segregating human allele of SPO11 modeled in mice disrupts timing and amounts of meiotic recombination, causing oligospermia and a decreased ovarian reserve†
T. N. Tran (2019)
Comparison between Slow Freezing and Vitrification for Human Ovarian Tissue Cryopreservation and Xenotransplantation
S. Lee (2019)
Novel dynamic culture system to support initiation of primordial follicle growth in prepubertal mouse ovaries.
K. Winkler-Crepaz (2014)
Maternal viral mimetic administration at the beginning of fetal hypothalamic nuclei development accelerates puberty in female rat offspring.
Pınar Çakan (2018)
Effect of a gonadotropin-releasing hormone analogue on cyclophosphamide-induced ovarian toxicity in adult mice
E. A. F. Kishk (2012)
Gonadotropin Control of Inhibin Secretion and the Relationship to Follicle Type and Number in the hpg Mouse1
Y. Wang (2005)
Differences in oocyte development and estradiol sensitivity among mouse strains.
M. E Pepling (2010)
Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: A stereological study
M. S. Mehranjani (2010)
The effect of a GnRH agonist on cryopreserved human ovarian grafts in severe combined immunodeficient mice.
T. Maltaris (2007)
A new model of reproductive aging: the decline in ovarian non-growing follicle number from birth to menopause.
K. Hansen (2008)
Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice
L. He (2017)
Effects of Cleome gynandra Linn: Leaf Extract on Ovarian Folliculogenesisof Albino Mice
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