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Localization Of BEN1-LIKE Protein And Nuclear Degradation During Development Of Metaphloem Sieve Elements In Triticum Aestivum L.

Jingtong Cai, Z. Zhang, Zhuqing Zhou, W. Yang, Y. Liu, Fangzhu Mei, G. Zhou, Likai Wang
Published 2015 · Biology, Medicine

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Metaphloem sieve elements (MSEs) in the developing caryopsis of Triticum aestivum L. undergo a unique type of programmed cell death (PCD); cell organelles gradually degrade with the MSE differentiation while mature sieve elements keep active. This study focuses on locating BEN1-LIKE protein and nuclear degradation in differentiating MSEs of wheat. Transmission electron microscopy (TEM) showed that nuclei degraded in MSE development. First, the degradation started at 2-3 days after flowering (DAF). The degraded fragments were then swallowed by phagocytic vacuoles at 4 DAF. Finally, nuclei almost completely degraded at 5 DAF. We measured the BEN1-LIKE protein expression in differentiating MSEs. In situ hybridization showed that BEN1-LIKE mRNA was a more obvious hybridization signal at 3-4 DAF at the microscopic level. Immuno-electron microscopy further revealed that BEN1-LIKE protein was mainly localized in MSE nuclei. Furthermore, MSE differentiation was tested using a TSQ Zn2+ fluorescence probe which showed that the dynamic change of Zn2+ accumulation was similar to BEN1-LIKE protein expression. These results suggest that nucleus degradation in wheat MSEs is associated with BEN1-LIKE protein and that the expression of this protein may be regulated by Zn2+ accumulation variation.
This paper references
Endonuclease genes up-regulated in tissues undergoing programmed cell death are expressed during male gametogenesis in barley.
G. Zaina (2003)
The Building and Application of Main Crop EST Analysis System. Dissertation, University of Chinese Academy of Agricultural Sciences, China
T. J. Feng (2004)
Degradation of chloroplast DNA in second leaves of rice (Oryza sativa) before leaf yellowing
Sodmergen (2005)
Programmed cell death of tracheary elements as a paradigm in plants
H. Fukuda (2004)
in leaves of barley plants under salt stress
K. Obara (2001)
Pollination induces mRNA poly(A) tail-shortening and cell deterioration in flower transmitting tissue.
H. Wang (1996)
A. Glücksmann (1951)
Characterization of nuclease activities and DNA fragmentation induced upon hypersensitive response cell death and mechanical stress
R. Mittler (2004)
ZEN1 Is a Key Enzyme in the Degradation of Nuclear DNA during Programmed Cell Death of Tracheary Elements
Hiroo Fukudaab (2002)
Programmed cell death: similarities and differences in animals and plants. A flower paradigm
M. Mea (2007)
S. Tabor (2001)
A unique program for cell death in xylem fibers of Populus stem.
C. Courtois-Moreau (2009)
Evidence of ceased programmed cell death in metaphloem sieve elements in the developing caryopsis of Triticum aestivum L.
Likai Wang (2008)
The phloem, a miracle of ingenuity
A. V. Bel (2003)
G. Zaina (2003)
Endonuclease genes upregulated in tissues undergoing programmed cell death are expressed during male gametogenesis
G. Zaina (2003)
Mannose induces an endonuclease responsible for DNA laddering in plant cells.
J. C. Stein (1999)
Enhanced expression of a nuclease gene in leaves of barley plants under salt stress.
Y. Muramoto (1999)
Ultrastructure and Its Function of Phloem Cell in Abdominal Vascular Bundle of Wheat Caryopsis
Z. Zhu (2004)
Microtubules and Sieve Plate Development in Differentiating Protophloem Sieve Elements of Triticum aestivum L.
E. Eleftheriou (1990)
Structure-function relationships during metaphloem sieve elements development in Triticum aestivum
C. N. Yang (2012)
Enhanced expression of a nuclease
Y. Muramoto (1999)
Study on programmed cell death and dynamic changes of starch accumulation in pericarp cells of Triticum aestivum L.*
Zhuqing Zhou (2009)
Nutrition of strawberry plants under controlled conditions: a) Effects of deficiencies of boron and certain other elements; b) Susceptibility to injury from sodium salts
D. R. Hoagland (1933)
A Gibberellin-induced Nuclease Is Localized in the Nucleus of Wheat Aleurone Cells Undergoing Programmed Cell Death*
F. Domínguez (2004)
Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death.
R. Mittler (1995)
Ultrastructural evidence for a dual function of the phloem and programmed cell death in the floral nectary of Digitalis purpurea.
K. Gaffal (2007)
Study on Programmed Cell Semi-Death of Sieve Elements in Root and Developing Caryopsis of Triticum aestivum L
W. L. Yang (2013)
Preliminary Research on Cathepsin B-like Protein in Triticum aestivum L. Caryopsis and Root
L. Yan (2013)
Identification of a nuclear-localized nuclease from wheat cells undergoing programmed cell death that is able to trigger DNA fragmentation and apoptotic morphology on nuclei from human cells.
F. Domínguez (2006)
The apoptosis endonucleases: cleaning up after cell death?
M. Peitsch (1994)
Programmed Cell Death in Plants.
R. Pennell (1997)
Expression analysis of the BFN1 nuclease gene promoter during senescence, abscission, and programmed cell death-related processes
Sarit Farage-Barhom (2008)
Development of protophloem in roots ofAegilops comosa var.thessalica. I. Differential divisions and pre-prophase bands of microtubules
E. Eleftheriou (2005)
Developmental Programmed Cell Death in Plants
Z. Bi (2005)
Direct evidence of active and rapid nuclear degradation triggered by vacuole rupture during programmed cell death in Zinnia.
K. Obara (2001)
Process of aerenchyma formation and reactive oxygen species induced by waterlogging in wheat seminal roots
Q. T. Xu (2013)
Senescence in Plant Development: The death of plants or plant parts may be of positive ecological or physiological value.
A. Leopold (1961)
A zinc-dependent nuclear endonuclease is responsible for DNA laddering during salt-induced programmed cell death in root tip cells of rice.
Ai-Liang Jiang (2008)
Ultrastructural Studies on the Sieve Elements in Root Protophleom of Arabidopsis thaliana
Zheng Xing (2003)
Development of protophloem in roots ofAegilops comosa var.thessalica. II. Sieve-element differentiation
E. Eleftheriou (2005)
Structure-function relationships during metaphloem sieve elements d evelopment in Triticum aestivum
C. N. Yang (2012)
Effects of Zn~(2+) on rice root tip cells programmed cell death under high salt stress or UV-induced
Z. Wei (2010)
BEN1 and ZEN1 cDNAs encoding S1‐type DNases that are associated with programmed cell death in plants 1
S. Aoyagi (1998)

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