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A Novel L1 Retrotransposon Marker For HeLa Cell Line Identification.

R. Rahbari, Tom Sheahan, V. Modes, P. Collier, C. Macfarlane, R. Badge
Published 2009 · Biology, Medicine

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The HeLa cell line is the oldest, most widely distributed, permanent human cell line. As a nearly ubiquitous inhabitant of laboratories using tissue culture techniques, its aggressive growth characteristics make it a problematic contaminant that can overgrow less robust cell lines. Consequently, HeLa contamination is common in both the research laboratory and cell line repository contexts, and its detection is hampered by the lack of a rapid, sensitive and robust assay. Here we report the development of a HeLa-specific DNA diagnostic test: a single duplex detection PCR assay targeting an L1 retrotransposon insertion. All HeLa clones from a geographically diverse panel were positive by this assay, and the particular L1 insertion we identified appears to be unique to the HeLa cell line. The assay can detect very low levels of HeLa contamination (<1%), and can be performed on un-purified cell pellets, allowing rapid routine screening.
This paper references
HeLa cultures defined.
W. Nelson-Rees (1976)
M Address Correspondence To Richard
HeLa cells 50 years on: the good, the bad and the ugly
J. Masters (2002)
The human genome browser at UCSC.
W. Kent (2002)
Genomic deletions and precise removal of transposable elements mediated by short identical DNA segments in primates.
L. N. van de Lagemaat (2005)
Initial sequencing and analysis of the human genome
International Human Genome Sequencing Consortium (2001)
Locomotory invasion of human cervical epithelium and avian fibroblasts by HeLa cells in vitro.
E. M. Stephenson (1982)
Characterization of terminal deletions at 7q32 and 22q13.3 healed by De novo telomere addition.
H. Varley (2000)
Curbing rampant cross-contamination and misidentification of cell lines.
R. Nardone (2008)
BLAT--the BLAST-like alignment tool.
W. Kent (2002)
Culture characteristics of four permanent lines of human cancer cells.
A. E. Moore (1955)
Human Population Genetic Structure and Diversity Inferred from Polymorphic L1(LINE-1) and Alu Insertions
D. Witherspoon (2006)
The costs of using unauthenticated, over-passaged cell lines: how much more data do we need?
Peyton Hughes (2007)
Widespread intraspecies cross‐contamination of human tumor cell lines arising at source
R. Macleod (1999)
ATLAS: a system to selectively identify human-specific L1 insertions.
R. Badge (2003)
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
T. Puck (1956)
Persistent use of “false” cell lines
M. Lacroix (2008)
Short tandem repeat profiling provides an international reference standard for human cell lines
J. Masters (2001)
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
T. Chen (1988)
Eradication of cross-contaminated cell lines: A call for action
R. Nardone (2007)

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Establishment of the cell line , HeLa-CD 14 , transfected with the human CD 14 gene
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Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity.
J. Oliveira (2017)
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Meng-ling Li (2020)
Tumoricidal effects of nanomaterials in HeLa cell line
M. Fakhar-e-Alam (2011)
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L. Feng (2014)
Design, semisynthesis and cytotoxic activity of novel ester derivatives of betulinic acid-1,2,4 oxadiazoles
Krishna Challa (2016)
Retroviral expression of human cystatin genes in HeLa cells.
Crystal M Diep (2015)
Reproducing Cells Is Nothing New—A Historical Prospective
M. Mitchell (2017)
methyl-ATAC-seq measures DNA methylation at accessible chromatin
Roman Spektor (2018)
Cell Type-Specific Modulation of Respiratory Chain Supercomplex Organization
Dayan Sun (2016)
Impact of pH and cell medium on the interaction of doxorubicin with lipoic acid cyclodextrin conjugate as the drug carrier
Olga Swiech (2020)
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J. Russo (2014)
Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (B). Efficiency of pharmacological action.
V. Dzmitruk (2015)
Moringa oleifera and vesicular stomatitis virus: A combination approach for the treatment of cervical cancers
A. Brown (2020)
Chemometric strategies to unmix information and increase the spatial description of hyperspectral images: a single-cell case study.
S. Piqueras (2013)
Observation of the Generation of ONOO- in Mitochondria under Various Stimuli with a Sensitive Fluorescence Probe.
H. Li (2017)
Facile synthesis of novel substituted aryl-thiazole (SAT) analogs via one-pot multi-component reaction as potent cytotoxic agents against cancer cell lines.
Salma Mirza (2017)
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R. Lynch (2015)
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