Online citations, reference lists, and bibliographies.
← Back to Search

Enhanced Cellular Uptake Of Aminosilane-coated Superparamagnetic Iron Oxide Nanoparticles In Mammalian Cell Lines

X. Zhu, Y. Wang, K. Leung, Siu-Fung Lee, F. Zhao, D. Wang, Josie M Y Lai, C. Wan, C. Cheng, A. Ahuja
Published 2012 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Purpose To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide (SPIO@SiO2-NH2) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 (SPIO@SiO2), dextran (SPIO@dextran), or bare SPIO in mammalian cell lines. Materials and methods Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7–15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 μg/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO nanoparticle on the cell viability of RAW 264.7 (mouse monocyte/macrophage) cells was also evaluated. Results Transmission electron microscopy demonstrated surface coating with SiO2-NH2, SiO2, and dextran prevented SPIO nanoparticle aggregation in DMEM culture medium. MCF-7, MDA-MB-231, and HT-29 cells failed to show notable iron uptake. For all the remaining six cell lines, Prussian blue staining and intracellular iron quantification demonstrated that SPIO@ SiO2-NH2 nanoparticles had the highest cellular uptake efficiency. SPIO@SiO2-NH2, bare SPIO, and SPIO@dextran nanoparticles did not affect RAW 264.7 cell viability up to 200 μg Fe/mL, while SPIO@SiO2 reduced RAW 264.7 cell viability from 10 to 200 μg Fe/mL in a dose-dependent manner. Conclusion Cellular uptake efficiency of SPIO nanoparticles depends on both the cell type and SPIO surface characteristics. Aminosilane surface coating enhanced the cellular uptake efficiency without inducing cytotoxicity in a number of cell lines.
This paper references
10.3346/jkms.2010.25.2.211
In vivo Tracking of Mesenchymal Stem Cells Labeled with a Novel Chitosan-coated Superparamagnetic Iron Oxide Nanoparticles using 3.0T MRI
A. M. Reddy (2010)
10.1021/jp807081b
Preparation and characterization of chemically functionalized silica-coated magnetic nanoparticles as a DNA separator.
Kiho Kang (2009)
10.1109/TMAG.1981.1061188
Preparation of aqueous magnetic liquids in alkaline and acidic media
R. Massart (1981)
Ceramic Transactions
Ed Clarke (1999)
10.1016/J.ADDR.2006.09.013
Recent advances in iron oxide nanocrystal technology for medical imaging.
C. Corot (2006)
Poly-L-Lysine induces fibrosis on alginate microcapsules via the induction of cytokines.
B. Strand (2001)
10.1080/02656730110095609
The effect of tumour size on ferromagnetic embolization hyperthermia in a rabbit liver tumour model
P. Moroz (2002)
10.1038/ncpcardio0659
Technology Insight: in vivo cell tracking by use of MRI
Walter J Rogers (2006)
Core-shell Fe 3 O 4 @SiO 2 nanoparticles synthesized with well-dispersed hydrophilic Fe 3 O 4 seeds. Nanoscale
C Hui (2011)
10.1016/j.saa.2010.04.004
Preparation and characterization of silica coated iron oxide magnetic nano-particles.
Y. Li (2010)
10.2147/ijn
科技期刊稿件处理的精细化管理——《International Journal of Nanomedicine〉〉投稿体会
鲁翠涛 (2014)
10.1007/s003300100908
Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging
Y. Wang (2014)
Preparation and biomedical application of a non-polymer coated superparamagnetic nanoparticle
Lin Du (2007)
10.1007/978-1-60761-609-2_7
Nanoshells for photothermal cancer therapy.
Jennifer G Morton (2010)
10.1002/jmri.22173
Low‐intensity pulsed ultrasound increases cellular uptake of superparamagnetic iron oxide nanomaterial: Results from human osteosarcoma cell line U2OS
Y. Wang (2010)
10.1021/nn200809t
Use of size and a copolymer design feature to improve the biodistribution and the enhanced permeability and retention effect of doxorubicin-loaded mesoporous silica nanoparticles in a murine xenograft tumor model.
Huan Meng (2011)
10.1124/jpet.106.101154
A Mechanistic Study of Enhanced Doxorubicin Uptake and Retention in Multidrug Resistant Breast Cancer Cells Using a Polymer-Lipid Hybrid Nanoparticle System
H. L. Wong (2006)
10.1021/MP0500014
Iron oxide nanoparticles for sustained delivery of anticancer agents.
Tapan Jain (2005)
10.1088/0957-4484/20/11/115103
The influence of surface functionalization on the enhanced internalization of magnetic nanoparticles in cancer cells.
Ángeles Villanueva (2009)
10.1016/j.biomaterials.2011.08.076
Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood.
Hengyi Xu (2011)
10.3978/j.issn.2223-4292.2011.08.03
Superparamagnetic iron oxide based MRI contrast agents: Current status of clinical application.
Y. Wáng (2011)
10.1007/s11671-010-9663-5
Highly Sensitive Fluorescence Probe Based on Functional SBA-15 for Selective Detection of Hg2+
X. Wang (2010)
10.1007/s12010-011-9383-z
Theranostic Applications of Nanomaterials in Cancer: Drug Delivery, Image-Guided Therapy, and Multifunctional Platforms
A. Fernandez-Fernandez (2011)
10.1148/RADIOL.2341031236
Migration of iron oxide-labeled human hematopoietic progenitor cells in a mouse model: in vivo monitoring with 1.5-T MR imaging equipment.
H. Daldrup-Link (2005)
10.2174/1389201043376526
Monitoring cell therapy using iron oxide MR contrast agents.
J. Bulte (2004)
Silica- and alkoxysilanecoated ultrasmall superparamagnetic iron oxide particles: a promising tool to label cells for magnetic resonance imaging
C Zhang (2007)
10.1016/j.biomaterials.2008.05.015
Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells.
D. Thorek (2008)
10.1007/S10856-007-3015-8
Study on the endocytosis and the internalization mechanism of aminosilane-coated Fe3O4 nanoparticles in vitro
Yong-jie Ma (2007)
10.1161/01.STR.0000131268.50418.b7
In Vivo Detection of Macrophages in Human Carotid Atheroma: Temporal Dependence of Ultrasmall Superparamagnetic Particles of Iron Oxide–Enhanced MRI
R. Trivedi (2004)
10.1039/c0cp00231c
Multilamellar liposomes entrapping aminosilane-modified maghemite nanoparticles: "magnetonions".
M. Meyre (2010)
10.1111/j.1365-2990.2004.00557.x
Imaging of iron oxide nanoparticles by MR and light microscopy in patients with malignant brain tumours
E. Neuwelt (2004)
10.1016/j.nano.2009.07.008
Development of multiple-layer polymeric particles for targeted and controlled drug delivery.
Bhanuprasanth Koppolu (2010)
MR imaging of relapsing multiple sclerosis patients using ultra-small-particle iron oxide and compared with gadolinium.
V. Dousset (2006)
Multilamellar liposomes entrapping aminosilane-modif ied maghemite nanoparticles
Me Meyre (2010)
10.1007/s00330-004-2405-2
Capacity of human monocytes to phagocytose approved iron oxide MR contrast agents in vitro
S. Metz (2004)
10.1093/JNCI/96.2.90
Improved Paclitaxel formulation hints at new chemotherapy approach.
K. Garber (2004)
10.2217/nnm.10.114
Magnetic nanoparticles in biomedicine: synthesis, functionalization and applications.
Reynolds A. Frimpong (2010)
10.1016/S0142-9612(02)00440-4
Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating.
C. Wilhelm (2003)
10.1016/j.addr.2010.05.006
Superparamagnetic iron oxide nanoparticles (SPIONs): development, surface modification and applications in chemotherapy.
M. Mahmoudi (2011)
10.1021/LA052006D
Investigation of the core-shell interface in gold@silica nanoparticles: a silica imprinting approach.
Saran Poovarodom (2005)
10.1002/ANIE.200602866
Magnetic nanoparticles: synthesis, protection, functionalization, and application.
A. Lu (2007)
10.1016/j.colsurfb.2009.01.021
Immobilization of albumin on aminosilane modified superparamagnetic magnetite nanoparticles and its characterization.
Keziban Can (2009)
10.1039/c0nr00497a
Core-shell Fe3O4@SiO2 nanoparticles synthesized with well-dispersed hydrophilic Fe3O4 seeds.
Chao Hui (2011)
10.1016/j.colsurfb.2009.03.004
Effect of surface charge of magnetite nanoparticles on their internalization into breast cancer and umbilical vein endothelial cells.
Tetsuya Osaka (2009)
10.1016/j.taap.2011.03.011
Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells.
A. Kunzmann (2011)
10.2147/IJN.S1608
Magnetic nanoparticles for gene and drug delivery
S. McBain (2008)
10.2217/17435889.3.4.579
Silica-based multimodal/multifunctional nanoparticles for bioimaging and biosensing applications.
Padmavathy Tallury (2008)
10.1021/la102447y
Chemical vapor deposition of three aminosilanes on silicon dioxide: surface characterization, stability, effects of silane concentration, and cyanine dye adsorption.
F. Zhang (2010)
10.1038/mt.2009.315
Mesenchymal stem cells expressing osteogenic and angiogenic factors synergistically enhance bone formation in a mouse model of segmental bone defect.
S. Kumar (2010)
10.1021/LA061879K
Silica- and alkoxysilane-coated ultrasmall superparamagnetic iron oxide particles: a promising tool to label cells for magnetic resonance imaging.
C. Zhang (2007)
10.1093/BRAIN/AWH191
In vivo MRI of brain inflammation in human ischaemic stroke.
A. Saleh (2004)
10.1166/JNN.2004.011
A novel fluorescent label based on organic dye-doped silica nanoparticles for HepG liver cancer cell recognition.
X. He (2004)
10.1016/0022-1759(86)90368-6
Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.
F. Denizot (1986)
Functionalisation of magnetic nanoparticles for applications in biomedicine : Biomedical applications of magnetic nanoparticles
C. Berry (2003)
10.1016/j.ijpharm.2010.03.061
Silica nanoparticle coated liposomes: a new type of hybrid nanocapsule for proteins.
Vellore J. Mohanraj (2010)
10.1021/la8032397
Contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles.
T. Tanaka (2009)
Core - shell Fe 3 O 4 @ SiO 2 nanoparticles synthesized with well - dispersed hydrophilic Fe 3 O 4 seeds
C Hui (2011)
In vitro labelling of mouse embryonic stem cells with SPIO nanoparticles.
J. Krejčí (2008)
10.1016/J.BIOMATERIALS.2004.05.022
Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles.
A. Gupta (2005)
10.1016/J.BIOS.2003.10.002
Real-time analysis of protein adsorption to a variety of thin films.
K. Sapsford (2004)
10.1002/chem.200901548
Durable mesenchymal stem cell labelling by using polyhedral superparamagnetic iron oxide nanoparticles.
Hao-Hao Wang (2009)
10.1165/AJRCMB.27.1.4790
Silica-induced apoptosis in murine macrophage: involvement of tumor necrosis factor-alpha and nuclear factor-kappaB activation.
E. Gozal (2002)



This paper is referenced by
10.2147/IJN.S57923
Physicochemical properties of surface charge-modified ZnO nanoparticles with different particle sizes
Kyoung-Min Kim (2014)
10.2147/IJN.S89679
Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
P. Santhosh (2015)
10.1039/c5nr03887a
Scientific and industrial challenges of developing nanoparticle-based theranostics and multiple-modality contrast agents for clinical application.
Yì-Xiáng J Wáng (2015)
10.4103/2349-3666.240591
Surface engineering of iron oxide nanoparticles for cancer therapy
Santosh L. Gawali (2017)
10.1088/0957-4484/25/5/055101
Enhanced blood-brain barrier transmigration using a novel transferrin embedded fluorescent magneto-liposome nanoformulation.
H. Ding (2014)
10.1186/s12951-015-0073-9
Characterization of interaction of magnetic nanoparticles with breast cancer cells
M. Calero (2015)
10.2147/IJN.S139011
Double-receptor-targeting multifunctional iron oxide nanoparticles drug delivery system for the treatment and imaging of prostate cancer
Md Shakir Uddin Ahmed (2017)
10.1002/jat.3367
Cytotoxicity and proliferative capacity impairment induced on human brain cell cultures after short‐ and long‐term exposure to magnetite nanoparticles
T. Coccini (2017)
10.1371/journal.pone.0085835
Toxicity Assessment of Silica Coated Iron Oxide Nanoparticles and Biocompatibility Improvement by Surface Engineering
M. A. Malvindi (2014)
10.3390/pharmaceutics12050424
In Vitro Intracellular Hyperthermia of Iron Oxide Magnetic Nanoparticles, Synthesized at High Temperature by a Polyol Process
C. Iacoviță (2020)
Title Nanoparticle-encapsulated chlorhexidine against oral bacterialbiofilms
C. Seneviratne (2014)
AWARD NUMBER: W81XWH-13-1-0288 TITLE: Microenvironment-Sensitive Multimodal Contrast Agent for Prostate Cancer Diagnosis
P. Investigator (2017)
10.3390/nano7080202
Versatility of Pyridoxal Phosphate as a Coating of Iron Oxide Nanoparticles
Débora Bonvin (2017)
10.1016/j.jconrel.2020.07.032
Endocytic trafficking of polymeric clustered superparamagnetic iron oxide nanoparticles in mesenchymal stem cells.
S. Lee (2020)
10.1007/s11356-016-7870-z
Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells
K. Srikanth (2016)
10.1016/j.mtcomm.2020.101470
Hydrophobically Modified Sodium Alginate Conjugated Plasmonic Magnetic Nanocomposites For Drug Delivery & Magnetic Resonance Imaging
Varun Arora (2020)
10.3978/j.issn.2223-4292.2015.03.12
Evaluation of biocompatible alginate- and deferoxamine-coated ternary composites for magnetic resonance imaging and gene delivery into glioblastoma cells.
K. Leung (2015)
10.1016/j.canlet.2013.04.032
Multifunctional superparamagnetic iron oxide nanoparticles: promising tools in cancer theranostics.
P. Santhosh (2013)
10.1002/tcr.201500259
Noble Metal-Iron Oxide Hybrid Nanomaterials: Emerging Applications.
K. Leung (2016)
10.1038/mp.2014.110
Imaging of activated complement using ultrasmall superparamagnetic iron oxide particles (USPIO) - conjugated vectors: an in vivo in utero non-invasive method to predict placental insufficiency and abnormal fetal brain development
G. Girardi (2015)
10.1016/j.ijpharm.2015.10.058
Recent advances in superparamagnetic iron oxide nanoparticles (SPIONs) for in vitro and in vivo cancer nanotheranostics.
G. Kandasamy (2015)
10.1016/B978-0-323-42867-5.00009-6
Role of nanomaterials in clinical dentistry
S. Krishnamurthy (2016)
10.2174/1381612811319370003
Recent Advances in Superparamagnetic Iron Oxide Nanoparticles for Cellular Imaging and Targeted Therapy Research
Y. Wang (2013)
10.1002/jat.3282
The responses of immune cells to iron oxide nanoparticles
Yaolin Xu (2016)
10.1039/C6TB01480A
Differential internalization of brick shaped iron oxide nanoparticles by endothelial cells.
Zhizhi Sun (2016)
Cell response to imaging contrast agents suggested for atherosclerotic plaque imaging
Amit Laskar (2013)
SPIONs on hiPSC-Di ff erentiated Endothelial Cells
Barbara Salingova (2019)
10.1002/em.21909
Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity
Vanessa Valdiglesias (2015)
10.1016/j.nano.2016.05.007
Physicochemical properties of engineered nanomaterials that influence their nervous system distribution and effects.
R. Yokel (2016)
10.1021/am300008x
Photocytotoxicity and magnetic relaxivity responses of dual-porous γ-Fe2O3@meso-SiO2 microspheres.
Shou-hu Xuan (2012)
10.1186/s13568-019-0857-7
Antifungal activity of silver nanoparticles in combination with ketoconazole against Malassezia furfur
Javier Mussin (2019)
10.1039/C3TB20090F
Folate-conjugated Fe3O4@SiO2@gold nanorods@mesoporous SiO2 hybrid nanomaterial: a theranostic agent for magnetic resonance imaging and photothermal therapy.
D. Wang (2013)
See more
Semantic Scholar Logo Some data provided by SemanticScholar