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Highly Efficient Mesenchymal Stem Cell Proliferation On Poly-ε-caprolactone Nanofibers With Embedded Magnetic Nanoparticles
J. Daňková, M. Buzgo, J. Vejpravová, S. Kubíčková, V. Sovkova, L. Vysloužilová, A. Mantlíková, A. Nečas, E. Amler
Published 2015 · Materials Science, Medicine
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In this study, we have developed a combined approach to accelerate the proliferation of mesenchymal stem cells (MSCs) in vitro, using a new nanofibrous scaffold made by needleless electrospinning from a mixture of poly-ε-caprolactone and magnetic particles. The biological characteristics of porcine MSCs were investigated while cultured in vitro on composite scaffold enriched with magnetic nanoparticles. Our data indicate that due to the synergic effect of the poly-ε-caprolactone nanofibers and magnetic particles, cellular adhesion and proliferation of MSCs is enhanced and osteogenic differentiation is supported. The cellular and physical attributes make this new scaffold very promising for the acceleration of efficient MSC proliferation and regeneration of hard tissues.
This paper references
Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
X. B. Zeng (2012)
Multilineage potential of adult human mesenchymal stem cells.
M. Pittenger (1999)
Superparamagnetic iron oxide nanoparticle targeting of MSCs in vascular injury. Biomaterials
J Riegler (2015)
In vitro toxicity of iron oxide nanoparticle: oxidative damages on Hep G2 cells.
L. Sadeghi (2015)
Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte-rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo.
E. Prosecká (2015)
Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors for prevention of incisional hernia formation
M. Plencner (2014)
Studies on Microstructural and Thermophysical properties of polymer nanocomposite based on polyphenylene oxide and Ferrimagnetic iron oxide
K. Agarwal (2011)
Polymeric Materials for Bone and Cartilage Repair
D. Puppi (2010)
10 submit your manuscript | www.dovepress.com Dovepress Dovepress Dovepress 7317 scaffold with magnetic particles for mesenchymal stem cell proliferation
Hyperpolarization of Human Mesenchymal Stem Cells in Response to Magnetic Force
G. Kirkham (2010)
Core/shell nanofibers with embedded liposomes as a drug delivery system.
A. Míčková (2012)
Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells
H. Luu (2007)
In Vivo Differentiation of Magnetically Labeled Mesenchymal Stem Cells Into Hepatocytes for Cell Therapy to Repair Damaged Liver
Sheng-hong Ju (2010)
Magnetite nanoparticles with no surface spin canting
A. G. Roca (2009)
Magnetic force-based mesenchymal stem cell expansion using antibody-conjugated magnetoliposomes.
A. Ito (2005)
The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles.
D. Huang (2009)
The return of a forgotten polymer : Polycaprolactone in the 21st century
M. Woodruff (2009)
Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells.
G. D’Ippolito (2006)
Potential of Magnetic Nanofiber Scaffolds with Mechanical and Biological Properties Applicable for Bone Regeneration
R. K. Singh (2014)
Magnetic micro- and nanoparticle mediated activation of mechanosensitive ion channels.
S. Hughes (2005)
Electrospinning: applications in drug delivery and tissue engineering.
T. J. Sill (2008)
Challenges in the development of magnetic particles for therapeutic applications
S. Barry (2008)
In vitro and in vivo toxicity of magnetic microspheres
U. Häfeli (1999)
Intracellular Hyperthermia for Cancer Using Magnetite Cationic Liposomes: Ex vivo Study
M. Yanase (1997)
Regulation of osteogenic differentiation during skeletal development.
Z. Deng (2008)
Magnetic biodegradable Fe3O4/CS/PVA nanofibrous membranes for bone regeneration.
Y. Wei (2011)
Increased osteoblast functions in the presence of hydroxyapatite-coated iron oxide nanoparticles.
N. Tran (2011)
Intracellular hyperthermia for cancer using magnetite cationic liposomes: in vitro study.
M. Shinkai (1996)
Superparamagnetic nano-composite scaffolds for promoting bone cell proliferation and defect reparation without a magnetic field
Kuilin Lai (2012)
Physical principles of electrospinning
D Lukáš (2009)
Superparamagnetic iron oxide nanoparticle targeting of MSCs in vascular injury.
J. Riegler (2013)
Magnetic particles for medical applications by glass crystallisation
R. N. Muller (2004)
Embedding of magnetic nanoparticles in polycaprolactone nanofiber scaffolds to facilitate bone healing and regeneration
Jacob T. Kannarkat (2010)
Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues.
A. Friedenstein (1968)
Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo
Friedrich Scherer (2002)
Pyroelectric Effect in Bone and Tendon
SIDNEY B. Lang (1966)
Osteogenic differentiation of MC3T3-E1 cells on poly(L-lactide)/Fe3O4 nanofibers with static magnetic field exposure.
Q. Cai (2015)
Generation of magnetic nonviral gene transfer agents and magnetofection in vitro
O. Mykhaylyk (2007)
Magnetic poly(epsilon-caprolactone)/ iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering
A Gloria (2013)
Preliminary analysis of magnetic particle techniques for activating mechanotransduction in bone cells
S. Cartmell (2002)
Synergic effect of magnetic nanoparticles on the electrospun aligned superparamagnetic nanofibers as a potential tissue engineering scaffold
H. Hu (2013)
Cobalt -silica magnetic nanoparticles with functional surfaces
M. L. Vadala (2005)
Greater osteoblast and mesenchymal stem cell adhesion and proliferation on titanium with hydrothermally treated nanocrystalline hydroxyapatite/magnetically treated carbon nanotubes.
M. Wang (2012)
Elastic three‐dimensional poly (ε‐caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells
M. Rampichova (2013)
Magnetic nanohydroxyapatite/PVA composite hydrogels for promoted osteoblast adhesion and proliferation.
R. Hou (2013)
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Magnetic field and nano-scaffolds with stem cells to enhance bone regeneration.
Y. Xia (2018)
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Z. Huang (2020)
Nanotechnology in Regenerative Medicine and Drug Delivery Therapy
H. Xu (2020)
Macrophage phenotypic mechanomodulation of enhancing bone regeneration by superparamagnetic scaffold upon magnetization.
Suisui Hao (2017)
An investigation on polycaprolactone/chitosan/Fe3O4 nanofibrous composite used for hyperthermia
Mehdi Hadjianfar (2019)
Poly(Caprolactone)‐Poly(N‐Isopropyl Acrylamide)‐Fe3O4 Magnetic Nanofibrous Structure with Stimuli Responsive Drug Release
Sanaz Gholami (2020)
Biodegradable Nanoparticles for Delivery of Therapeutics in CNS Infection
Catherine Demarino (2016)
Fabrication and physicochemical characterization of a novel magnetic nanocomposite scaffold: Electromagnetic field effect on biological properties.
Elmira Moradian (2020)
A Mini-Review: Needleless Electrospinning of Nanofibers for Pharmaceutical and Biomedical Applications
Ioannis Partheniadis (2020)
Protein Corona of Magnetic Hydroxyapatite Scaffold Improves Cell Proliferation via Activation of Mitogen-Activated Protein Kinase Signaling Pathway.
Y. Zhu (2017)
A Review of Nanofiber Shish Kebabs and Their Potential in Creating Effective Biomimetic Bone Scaffolds
Aria C. Attia (2018)
Magnetically Actuated Scaffolds to Enhance Tissue Regeneration
H. Xu (2020)