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Permeation Of PLGA Nanoparticles Across Different In Vitro Models.

L. Nkabinde, L. Shoba-Zikhali, B. Semete-Makokotlela, L. Kalombo, H. Swai, R. Hayeshi, B. Naicker, T. Hillie, J. Hamman
Published 2012 · Materials Science, Medicine

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Many drug delivery systems have indicated improvement in delivery of various drug molecules and among these biodegradable and biocompatible polymers such as poly(D,L-lactide-co-glycolide) (PLGA) have been shown to enhance intracellular uptake of drug candidates when formulated as nanoparticles. PLGA nanoparticles were prepared by means of a double emulsion solvent evaporation technique and evaluated in terms of size, encapsulation efficiency, surface charge, isoniazid release and in vitro transport. The nanoparticles have an average size of 237 nm and were previously shown to be distributed in several tissues after oral administration without triggering an immune response. This study focussed on the in vitro permeation of the PLGA nanoparticles across different membranes and showed that although Rhodamine 6G-labelled nanoparticles are efficiently delivered across the intestinal epithelium, its epithelial permeability changes when a drug such as isoniazid is encapsulated. Future studies should focus on ways to optimise PLGA nanoparticle delivery when a drug such as isoniazid is encapsulated for instance by coating with polymers such as polyethylene glycol.
This paper references
Tuberculosis in sub-Saharan Africa: opportunities, challenges, and change in the era of antiretroviral treatment
E. Corbett (2006)
Magnetically directed poly(lactic acid) 90Y-microspheres: novel agents for targeted intracavitary radiotherapy.
U. Häfeli (1994)
Hyperthermia enables tumor-specific nanoparticle delivery: effect of particle size.
G. Kong (2000)
The preparation of sub-200 nm poly(lactide-co-glycolide) microspheres for site-specific drug delivery
P. Scholes (1993)
Evaluation of the cytotoxicity effect of dimethyl sulfoxide (DMSO) on Caco2/TC7 colon tumor cell cultures.
Georges Da Violante (2002)
Comparison of the Permeability Characteristics of a Human Colonic Epithelial (Caco-2) Cell Line to Colon of Rabbit, Monkey, and Dog Intestine and Human Drug Absorption
W. Rubas (2004)
Nanoparticles based on PLGA and its co‐polymer: An overview
Muthu (2009)
Evaluation of ciprofloxacin-loaded Eudragit RS100 or RL100/PLGA nanoparticles.
K. Dillen (2006)
Poly(α-hydroxyacids) for application in the spinal cord: Resorbability and biocompatibility with adult rat schwann cells and spinal cord
S. Gautier (1998)
Active secretion and enterocytic drug metabolism barriers to drug absorption.
V. J. Wacher (2001)
Gradient copolymers
U. Beginn (2008)
Use of Transporter Knockdown Caco-2 Cells to Investigate the In Vitro Efflux of Statin Drugs
Jibin Li (2011)
Targeting cancer cells using PLGA nanoparticles surface modified with monoclonal antibody.
P. Kocbek (2007)
Physicochemical aspects of drug delivery and release from polymer-based colloids
Lin Yang (2000)
Influence of experimental parameters on the characteristics of poly(lactic acid) nanoparticles prepared by a double emulsion method.
M. Zambaux (1998)
Poly(hydroxy acids) in drug
K. Juni (1987)
Oral Drug Delivery in Drug Delivery and Targeting; Hillery
V.H.L. Lee (2001)
Formulation optimization of etoposide loaded PLGA nanoparticles by double factorial design and their evaluation.
K. S. Yadav (2010)
Poly(alpha-hydroxyacids) for application in the spinal cord: resorbability and biocompatibility with adult rat Schwann cells and spinal cord.
S. Gautier (1998)
Mechanisms of isoniazid release from poly(d,l-lactide-co-glycolide) matrices prepared by dry-mixing and low density polymeric foam methods.
Y. Hsu (1996)
In vivo evaluation of the biodistribution and safety of PLGA nanoparticles as drug delivery systems.
B. Semete (2010)
A new double emulsion solvent diffusion technique for encapsulating hydrophilic molecules in PLGA nanoparticles.
Einat Cohen-Sela (2009)
Effect of acidic pH on PLGA microsphere degradation and release.
B. S. Zolnik (2007)
Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles.
Donald E. Owens (2006)
Drug absorption in vitro model: filter-immobilized artificial membranes. 2. Studies of the permeability properties of lactones in Piper methysticum Forst.
A. Avdeef (2001)
Permeability enhancing effects of the alkylglycoside, octylglucoside, on insulin permeation across epithelial membrane in vitro.
P. P. Tirumalasetty (2006)
Oral Delivery of Peptide Drugs
J. Hamman (2012)
Targeting Receptors, Transporters and Site of Absorption to Improve Oral Drug Delivery
J.H. Hamman (2007)
Does inhibition of P-glycoprotein lead to drug-drug interactions?
D. Balayssac (2005)
Role of individual drugs in the chemotherapy of tuberculosis.
D. Mitchison (2000)
Treatment of tuberculosis: present status and future prospects.
Philip C Onyebujoh (2005)
Caco-2 monolayers in experimental and theoretical predictions of drug transport.
P. Artursson (2001)
Single and double emulsion manufacturing techniques of an amphiphilic drug in PLGA nanoparticles: formulations of mithramycin and bioactivity.
Einat Cohen-Sela (2009)
The Influence of Donor and Reservoir Additives on Caco-2 Permeability and Secretory Transport of HIV Protease Inhibitors and Other Lipophilic Compounds
B. Aungst (2004)
Regulation of c‐myc expression by sodium butyrate in the colon carcinoma cell line Caco‐2
A. Souleimani (1993)
Poster Presentation at FASEB Meeting, Anaheim, California
M. Swiderek (1994)
A biophysical model of passive and polarized active transport processes in Caco-2 cells: approaches to uncoupling apical and basolateral membrane events in the intact cell.
N. F. Ho (1995)
Magnetic Microspheres and Tissue Model Studies for Therapeutic Applications
N. Ramachandran (2004)
Preparation of multi-phase microspheres of poly(D,L-lactic acid) and poly(D,L-lactic-co-glycolic acid) containing a W/O emulsion by a multiple emulsion solvent evaporation technique.
M. Iwata (1992)
Factors Affecting the Formation of the Monomer Sequence along Styrene/Methyl Methacrylate Gradient Copolymer Chains
L. Wang (2009)
Liposomes as delivery agents for medical imaging.
V. Torchilin (1996)
Poly(hydroxy acids) in drug delivery.
K. Juni (1987)
Lipidic vector systems for gene transfer.
R. Lee (1997)
Evaluating Barriers to Bioavailability in Vivo: Validation of a Technique for Separately Assessing Gastrointestinal Absorption and Hepatic Extraction
L. Letendre (2004)
Size-dependent extravasation and interstitial localization of polyethyleneglycol liposomes in solid tumor-bearing mice.
O. Ishida (1999)
Global Tuberculosis Control 2011, WHO Report 2011
D. Behera (2012)
Targeting intracellular targets.
J. Panyam (2004)

This paper is referenced by
In vitro assays for hazard identification of nanoparticles
I. Rietjens (2011)
Lipid regulation of placental mycotoxin transport
J. Szilagyi (2018)
Placental BCRP/ABCG2 Transporter Prevents Fetal Exposure to the Estrogenic Mycotoxin Zearalenone
J. Szilagyi (2019)
The development and evaluation of a novel hybrid PLGA nanoparticle-Pheroid® with the potential to improve tuberculosis therapy
Madichaba Phuthi Chelopo-Mgobozi (2018)
Translocation of differently sized and charged polystyrene nanoparticles in in vitro intestinal cell models of increasing complexity
A. P. Walczak (2015)
Core–shell drug carriers: liposomes, polymersomes, and niosomes
N. Dan (2017)
Orally disintegrating tablets: formulation development, novel engineering solutions and fixed dose combinations
T. Dennison (2017)
Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles
A. P. Walczak (2015)
A Physiologically Based Pharmacokinetic Model of Isoniazid and Its Application in Individualizing Tuberculosis Chemotherapy
H. Cordes (2016)
Cyclosporine A Loaded PLGA Nanoparticles for Dry Eye Disease: In Vitro Characterization Studies
Vijay D. Wagh (2014)
Efficiency of gentamicin loaded in bacterial polysaccharides microcapsules against intracellular Gram-positive and Gram-negative invasive pathogens.
C. Croitoru (2015)
Effect of particle size and surface charge of nanoparticles in penetration through intestinal mucus barrier
Sony Priyanka Bandi (2020)
Progress and future of in vitro models to study translocation of nanoparticles
H. Braakhuis (2015)
Chitosan complexed carboxymethylated iota-carrageenan oral insulin particles: Stability, permeability and in vivo evaluation
P. Sahoo (2019)
Nimesulide-loaded nanoparticles for the potential coadjuvant treatment of prostate cancer.
C. Huerta (2015)
Efficiency of gentamicin loaded in bacterial polysaccharides microcapsules against intracellular Gram-positive and Gram-negative invasive pathogens
C. Davila (2015)
Anandamide down‐regulates placental transporter expression through CB2 receptor‐mediated inhibition of cAMP synthesis
J. Szilagyi (2019)
Chapter 16 – Gastrointestinal System
M. Jepson (2017)
Polymeric Microporous Nanofilms as Smart Platforms for in Vitro Assessment of Nanoparticle Translocation and Caco-2 Cell Culture
L. Ricotti (2016)
Effects of food‐borne nanomaterials on gastrointestinal tissues and microbiota
H. Bouwmeester (2018)
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