Online citations, reference lists, and bibliographies.
← Back to Search

Altered Activity Of Peroxidase And Oxalate Oxidase Influences Lignification In Transgenic Tobacco

Emma Burbridge, S. Rasmussen, F. Bernier, B. K. Kristensen, P. McCabe, P. Dix
Published 2014 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Peroxidase and hydrogen peroxide both play important roles in the final stages of the lignification pathway. Peroxidase, in the presence of H2O2 catalyses the oxidation of monolignols to give lignin. In order to examine this process we looked at lignification in transgenic tobacco plants expressing a barley peroxidase gene, HvPrx8, either alone or in combination with a wheat germin gene, g.f 2.8, which encodes oxalate oxidase, thereby providing a source of H2O2. Ele- vated activity of the antioxidant ascorbate peroxidase was found in plants expressing oxalate peroxidase and was greatly increased by co-expression with the barley peroxidase, although the latter had no effect when expressed alone. An in- crease was observed in the oxidation of the lignin monomer, syringaldazine in cell lines over-expressing barley peroxi- dase, while a decrease was observed in double transformants. Plants over-expressing barley peroxidase have elevated lev- els of lignin deposition compared to that of wild type tobacco plants. Over-expression of the individual enzymes was also shown to enhance heat-induced programmed cell death (PCD) in cell suspension cultures, an effect which was greatly re- duced in the double-expressing lines.
This paper references
10.1023/A:1006110223774
Signals controlling the expression of cytosolic ascorbate peroxidase during pathogen-induced programmed cell death in tobacco
R. Mittler (2004)
10.1046/J.1365-313X.1999.00403.X
Cell wall alterations and localized accumulation of feruloyl-3-methoxytyramine in onion epidermis at sites of attempted penetration by Botrytis allii are associated with actin polarisation, peroxidase activity and suppression of flavonoid biosynthesis
Sarah R. McLusky (1999)
10.3923/JPS.2011.1.13
Increased Tolerance to Abiotic Stresses in Tobacco Plants Expressing a Barley Cell Wall Peroxidase
Deborah Vicuna (2011)
10.1042/BJ3300115
Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defence gene expression in Arabidopsis suspension cultures.
R. Desikan (1998)
10.1104/PP.94.2.485
In vitro oxidation of indoleacetic Acid by soluble auxin-oxidases and peroxidases from maize roots.
R. Beffa (1990)
10.1007/s11103-011-9784-y
Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation
Miranda Poage (2011)
10.1093/JXB/ERG253
Three differentially expressed basic peroxidases from wound-lignifying Asparagus officinalis.
K. B. Holm (2003)
10.1111/j.1365-313x.2001.00930.x
Cell death of barley aleurone protoplasts is mediated by reactive oxygen species.
P. Bethke (2001)
10.1007/BF00027212
Plant peroxidases—an organismic perspective
B. Siegel (2004)
10.1023/A:1026500810877
Programmed cell death in cell cultures
P. McCabe (2004)
10.1104/pp.107.4.1091
Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings
B. Dumas (1995)
10.1104/pp.114.4.1187
Characterization of Antisense Transformed Plants Deficient in the Tobacco Anionic Peroxidase
L. M. Lagrimini (1997)
10.1016/0885-5765(92)90031-P
cDNA cloning and characterization of two barley peroxidase transcripts induced differentially by the powdery mildew fungus Erysiphe graminis
H. Thordal-Christensen (1992)
10.1111/j.1467-7652.2011.00611.x
Tobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione-S-transferase, exhibit altered anti-oxidant metabolism and improved abiotic stress tolerance.
Bénédicte Le Martret (2011)
10.1016/S0168-9452(01)00427-7
Characterisation of and changes to pro and anti-oxidant enzyme activities during the hypersensitive reaction in lettuce (Lactuca sativa L.)
C. Bestwick (2001)
10.1016/S0168-9452(98)00034-X
Oxidative events during programmed cell death of daylily (Hemerocallis hybrid) petals
Tadas Panavas (1998)
10.1023/A:1005745015962
Regulated expression of a wheat germin gene in tobacco: oxalate oxidase activity and apoplastic localization of the heterologous protein
A. Berna (2004)
10.1104/PP.120.2.501
Barley coleoptile peroxidases. Purification, molecular cloning, and induction by pathogens.
B. K. Kristensen (1999)
10.1111/J.1399-3054.1962.TB08052.X
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)
10.1104/PP.118.3.1067
Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a nonhost hypersensitive reaction in lettuce
Bestwick (1998)
10.1104/PP.97.1.366
Germin-Like Polypeptides Increase in Barley Roots during Salt Stress.
W. Hurkman (1991)
10.1046/J.1365-313X.1997.12020267.X
A programmed cell death pathway activated in carrot cells cultured at low cell density
P. McCabe (1997)
10.1016/S0168-9452(96)04554-2
Expression of a defence-related intercellular barley peroxidase in transgenic tobacco
B. K. Kristensen (1997)
10.1007/BF01974090
Reduced lignin in transgenic plants containing a caffeic acidO-methyltransferase antisense gene
W. Ni (2005)
10.1016/S0176-1617(89)80127-0
On Substrate Specificity of Peroxidases Involved in the Lignification Process
A. Pang (1989)
10.1093/OXFORDJOURNALS.PCP.A029557
Induction of Rice Cytosolic Ascorbate Peroxidase mRNA by Oxidative Stress; the Involvement of Hydrogen Peroxide in Oxidative Stress Signalling
S. Morita (1999)
10.1104/PP.126.3.1012
Phenol-oxidizing peroxidases contribute to the protection of plants from ultraviolet radiation stress.
M. Jansen (2001)
10.1016/S0176-1617(11)80605-X
Cell-Wall-Associated Peroxidases and Lignification during Growth of Flax Fibres
G. McDougall (1991)
10.1104/PP.124.1.21
Role of reactive oxygen intermediates and cognate redox signaling in disease resistance.
John J. Grant (2000)
10.1105/tpc.2.1.7
Peroxidase-Induced Wilting in Transgenic Tobacco Plants.
L. M. Lagrimini (1990)
10.1104/PP.122.4.1119
A tomato peroxidase involved in the synthesis of lignin and suberin.
M. Quiroga (2000)
10.1104/PP.70.4.1132
Role of Peroxidase in Lignification of Tobacco Cells : II. Regulation by Phenolic Compounds.
M. Maeder (1982)
10.1023/A:1006123432157
Regulation by biotic and abiotic stress of a wheat germin gene encoding oxalate oxidase, a H2O2-producing enzyme
A. Berna (2004)
10.1016/S0167-4838(00)00017-0
Characterization of isoperoxidase-B2 inactivation in etiolated Lupinus albus hypocotyls.
J. Hernández-Ruiz (2000)
10.1046/J.1365-313X.1995.08010139.X
Germin‐like oxalate oxidase, a H2O2‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus
Z. Zhang (1995)
10.1104/PP.118.1.125
Purification and characterization of peroxidases correlated with lignification in poplar xylem.
J. H. Christensen (1998)
10.1016/S0044-328X(83)80109-3
Some Properties of Syringaldazine Oxidase, a Peroxidase Specifically Involved in the Lignification Processes
R. Goldberg (1983)
10.1146/ANNUREV.ARPLANT.47.1.299
XYLOGENESIS: INITIATION, PROGRESSION, AND CELL DEATH.
H. Fukuda (1996)
10.1146/ANNUREV.ARPLANT.49.1.249
ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control.
G. Noctor (1998)
10.1016/J.PLANTSCI.2006.03.004
Use of cell morphology to evaluate the effect of a peroxidase gene on cell death induction thresholds in tobacco
Emma Burbridge (2006)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1111/J.1399-3054.1989.TB06179.X
Physiological significance of the binding of acidic isoperoxidases to cell walls of lupin
A. Barceló (1989)
10.1111/J.1399-3054.1992.TB04728.X
Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plants
K. Asada (1992)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar