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D-amino Acid Oxidase-nanoparticle System: A Potential Novel Approach For Cancer Enzymatic Therapy.
A. Bava, Rosalba Gornati, Francesca Cappellini, Laura Caldinelli, L. Pollegioni, G. Bernardini
Published 2013 · Materials Science, Medicine
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AIM The authors propose a new magnetic nanoparticle-enzyme system for cancer therapy capable of targeting the enzyme and consequently decreasing the adverse effects, meanwhile improving the patient's life quality. MATERIALS & METHODS The authors have functionalized Fe3O4 nanoparticles with 3-amino-propyltriethoxysilane (APTES) and conjugated it to yeast D-amino acid oxidase (DAAO) by coupling this with glutaraldehyde. RESULTS & CONCLUSION The authors have tested the Fe3O4-APTES-DAAO system on three tumor cell lines. Exposed cells show, at the electron microscope level, nanoparticles on the surface of the plasma membrane and inside endocytic vesicles. Fe3O4-APTES-DAAO caused a substantial decrease of cell viability greatly augmented when D-alanine, a DAAO substrate, was added. Fe3O4-APTES-DAAO was demonstrated to be more effective than free DAAO, confirming the validity of the system in cancer therapy.
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