← Back to Search
Development Of Dual Toxoid-loaded Layersomes For Complete Immunostimulatory Response Following Peroral Administration.
H. Harde, Krupa Siddhapura, A. Agrawal, S. Jain
Published 2015 · Medicine
Download PDFAnalyze on Scholarcy
AIM Present study reports the development of divalent vaccine with enhanced protection, permeation and presentation following peroral immunization. MATERIALS & METHODS Layersomes were prepared by layer-by-layer tuning of polyelectrolytes on liposomes template. The developed system was evaluated for in vitro stability of antigen and layersomes, cell-based assays and immunization experiments in mice. RESULTS Layersomes exhibited enhanced stability in simulated biological fluids, still preserving the integrity, biological activity and conformational stability of toxoids. Layersomes also exhibited complete and protective (>0.1 IU/ml) immunostimulatory response include serum IgG titer, mucosal sIgA titer and cytokines (IL-2 and IFN-γ) levels following peroral administration. CONCLUSION The positive findings of proposed strategy are expected to contribute significantly in the field of stable liposomes technology and peroral immunization.
This paper references
Trilateral '3P' Mechanics of Stabilized Layersomes Technology for Efficient Oral Immunization.
H. Harde (2015)
Molecular basis of the mucosal immune system: from fundamental concepts to advances in liposome-based vaccines.
Shailja Tiwari (2010)
Correlates of Protection Induced by Vaccination
S. Plotkin (2010)
Tetanus Toxoids Loaded Glucomannosylated Chitosan Based Nanohoming Vaccine Adjuvant with Improved Oral Stability and Immunostimulatory Response
H. Harde (2014)
Improved stability and antidiabetic potential of insulin containing folic acid functionalized polymer stabilized multilayered liposomes following oral administration.
A. Agrawal (2014)
Chitosan nanoparticles for oral drug and gene delivery
Katherine Bowman (2006)
Freeze-drying of nanoparticles: formulation, process and storage considerations.
W. Abdelwahed (2006)
The quantitative estimation of diphtheria and tetanus toxoids. 1. The flocculation test and the Lf-unit.
J. Lyng (1987)
Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach.
A. des Rieux (2006)
Vaccination Strategies for Mucosal Immune Responses
P. Ogra (2001)
Design opportunities for actively targeted nanoparticle vaccines.
T. Fahmy (2008)
Dendritic-cell immunotherapy: from ex vivo loading to in vivo targeting
P. Tacken (2007)
Improving the reach of vaccines to low-resource regions, with a needle-free vaccine delivery device and long-term thermostabilization.
X. Chen (2011)
Co-encapsulation of tamoxifen and quercetin in polymeric nanoparticles: implications on oral bioavailability, antitumor efficacy, and drug-induced toxicity.
A. Jain (2013)
A single administration of tetanus toxoid in biodegradable microspheres elicits T cell and antibody responses similar or superior to those obtained with aluminum hydroxide.
Y. Men (1995)
Development of stabilized glucomannosylated chitosan nanoparticles using tandem crosslinking method for oral vaccine delivery.
H. Harde (2014)
Folate-decorated PLGA nanoparticles as a rationally designed vehicle for the oral delivery of insulin.
S. Jain (2012)
Biologically erodable microspheres as potential oral drug delivery systems
E. Mathiowitz (1997)
Development of pH-sensitive Insulin Nanoparticles using Eudragit L100-55 and Chitosan with Different Molecular Weights
M. Jelvehgari (2010)
Alginate/Chitosan Nanoparticles are Effective for Oral Insulin Delivery
B. Sarmento (2007)
In vivo evidence of oral vaccination with PLGA nanoparticles containing the immunostimulant monophosphoryl lipid A.
Federica Sarti (2011)
Nanocarriers for Transmucosal Vaccine Delivery
S. Jain (2011)
Chitosan microparticles for mucosal vaccination against diphtheria: oral and nasal efficacy studies in mice.
I. M. van der Lubben (2003)
Vaccines and vaccination.
G. Ada (2001)
Oral delivery of doxorubicin using novel polyelectrolyte-stabilized liposomes (layersomes).
S. Jain (2012)
Tetanus toxoid and synthetic malaria antigen containing poly(lactide)/poly(lactide-co-glycolide) microspheres : importance of polymer degradation and antigen release for immune response
C. Thomasin (1996)
Polyelectrolyte stabilized multilayered liposomes for oral delivery of paclitaxel.
S. Jain (2012)
Immunogenicity and protection in small-animal models with controlled-release tetanus toxoid microparticles as a single-dose vaccine.
M. Singh (1997)
DDSolver: An Add-In Program for Modeling and Comparison of Drug Dissolution Profiles
Y. Zhang (2010)
Host defense mechanisms in the gastrointestinal tract.
W. Walker (1976)
Solid lipid nanoparticles: an oral bioavailability enhancer vehicle
H. Harde (2011)
Improved stability and immunological potential of tetanus toxoid containing surface engineered bilosomes following oral administration.
S. Jain (2014)
PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination.
M. Garinot (2007)
Oral mucosal immunization using glucomannosylated bilosomes.
S. Jain (2014)
This paper is referenced by
Correction: Folate appended chitosan nanoparticles augment the stability, bioavailability and efficacy of insulin in diabetic rats following oral administration
A. Agrawal (2015)
S. Rosales-Mendoza (2019)
Recent developments in nanocarrier-aided mucosal vaccination.
Olga Kammona (2017)
Polymer-lipid hybrid systems: merging the benefits of polymeric and lipid-based nanocarriers to improve oral drug delivery
Shasha Rao (2016)
2 – Supramolecular Systems
M. D. Moraes (2017)
Multifaceted applications of bile salts in pharmacy: an emphasis on nanomedicine
Y. S. Elnaggar (2015)
Nanovaccines: An Innovative Technology to Fight Human and Animal Diseases
Sergio Rosales-Mendoza (2019)
Mucosal Vaccine Development Based on Liposome Technology
V. Bernasconi (2016)
Preformulation Challenges: The Concept Behind the Selection, Design and Preparation of Nanoformulations
K. K. Patel (2020)