← Back to Search
Extra-cellular Chromate-reducing Activity Of The Yeast Cultures
H. Ksheminska, T. Honchar, G. Gayda, M. Gonchar
Published 2006 · Chemistry
Download PDFAnalyze on Scholarcy
This paper reports on the experimental data supporting an essential role of extra-cellular reduction in chromate detoxification by baker’s and non-conventional yeasts. A decrease of chromate content in the yeast culture coincides with an increase of Cr(III) content in extra-cellular liquid. At these conditions, cell-bound chromium level was insignificant and a dominant part of extra-cellular Cr(III) species was detected in the reaction with chromazurol S only after mineralization of the cell-free samples. This phenomenon of chromium “disappearance” can be explained by the formation of Cr(III) stable complexes with extra-cellular yeast-secreted components which are “inaccessible” in the reaction with chromazurol S without mineralization. It was shown that increasing sucrose concentration in a growth medium resulted in an increase of chromate reduction. A strong inhibition of chromate reduction by 0.25 mM sodium azide, a respiration inhibitor and a protonophore, testifies that extra-cellular chromate detoxification depends on energetic status of the yeast cells. It was shown that Cr(III)-biochelates produced in extra-cellular medium are of a different chemical nature and can be separated into at least two components by ion-exchange chromatography on anionit Dowex 1x10. A total yield of the isolated Cr(III)-biocomplexes is approximately 65 % (from initial level of chromate) with a relative molar ratio 8:5.
This paper references
and S . Silver : “ Chromium reduction in Pseudomonas putida ”
Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae.
H. Cherest (1997)
Studies on the genotoxicity of chromium: from the test tube to the cell
R. Codd (2001)
Distribution and fate of hydrochlorothiazide-H3.
H. Sheppard (1960)
Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans.
J. Mazoch (2004)
Seguin Hen (1824)
Membrane-bound respiratory system of Enterobacter cloacae strain HO1 grown anaerobically with chromate.
P. C. Wang (1991)
Cr(VI) reduction in a chromate-resistant strain of Candida maltosa isolated from the leather industry
Rocío Ramírez-Ramírez (2004)
Potential hazards of hexavalent chromate in our drinking water.
M. Costa (2003)
Imbalabce Compensation of Magnetic Bearing System Using Discrete Time Q-Parameterization Control
J. Lee (1997)
Über die reaktion von chrom mit diphenylcarbazid und diphenylcarbazon
H. Marchart (1964)
Hexavalent chromium uptake by sensitive and tolerant mutants of Schizosaccharomyces pombe.
K. Czakó-Vér (1999)
Effects of hexavalent chromium on the survival and cell cycle distribution of DNA repair-deficient S. cerevisiae.
T. O’Brien (2002)
Hexavalent chromium stimulation of riboflavin synthesis in flavinogenic yeast
D. Fedorovych (2004)
Martin P. Catherwood (1824)
D. Saleh (2001)
Pulyaeva: “A spectrophotometric study of complexation between chromium and chromazurol S
I.V.R.P. Pantaler (1985)
Marchart : “ Über die Reaktion von Chrom mit Diphenylcarbazid und Diphenyl - carbazon ”
A. E. Greenberg (1964)
Chromium(III) and (VI) tolerance and bioaccumulation in yeast: a survey of cellular chromium content in selected strains of representative genera
H. Ksheminska (2005)
Lohmarer Stadtgeschichte (2005)
Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction.
D. Ackerley (2004)
A New Function of the Desulfovibrio vulgaris Hildenborough [Fe] Hydrogenase in the Protection against Oxidative Stress*
Marjorie Fournier (2004)
A . Dolla : “ A new function of the Desulfovibrio vulgaris Hildenborough [ Fe ] hydrogenase in the protection against oxidative stress ”
Z. Dermoun Fournier (2004)
Vibrio harveyi Nitroreductase Is Also a Chromate Reductase
Young Hak Kwak (2003)
Chromate sensitivity in fission yeast is caused by increased glutathione reductase activity and peroxide overproduction
M. Pesti (2002)
BETA-PROPIOLACTONE DECONTAMINATION OF SIMIAN VIRUS-40 AS DETERMINED BY A RAPID FLUORESCENT-ANTIBODY ASSAY.
S. I. Levine (1965)
Oxidative protein damage causes chromium toxicity in yeast.
Edward R Sumner (2005)
M. Sankar (1824)
Pro‐oxidative vs antioxidative properties of ascorbic acid in chromium(VI)‐induced damage: an in vivo and in vitro approach
B. Poljsak (2005)
Stress response of yeast candida intermedia to Cr(VI)
P. Jamnik (2003)
Chromate-Reducing Properties of Soluble Flavoproteins from Pseudomonas putida and Escherichia coli
D. Ackerley (2004)
深田 悟 (2006)
Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe
Z. Gazdag (2003)
Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase
C. H. Park (2000)
Standard Methods for the Examination of Water and Wastewater seventh edition
A. E. Greenberg (2013)
Chromium reduction in Pseudomonas putida.
Y. Ishibashi (1990)
Kinetic studies on the electron transfer between bacterial c-type cytochromes and metal oxides
E. Lojou (1998)
Effect of hexavalent chromium on eukaryotic plasma membrane studied by EPR spectroscopy.
J. Belágyi (1999)
Saskia Bonjour (1824)
Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions.
P. C. Wang (1989)
Interrelations of the yeast Candida utilis and Cr(VI): metal reduction and its distribution in the cell and medium
O. Muter (2001)
John B. Shoven (1824)
A. Spring (2005)
A. Kumar (1824)
A bacterial flavin reductase system reduces chromate to a soluble chromium(III)-NAD(+) complex.
G. Puzon (2002)
This paper is referenced by
Biological Cr(VI) reduction in a trickling filter under continuous operation with recirculation
E. Dermou (2008)
Reduction of chromate and carotene-synthesizing activity of selenite-resistant mutants of the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)
H. I. Nechay (2009)
A review of chromite mining in Sukinda Valley of India: impact and potential remediation measures
S. Nayak (2020)
Biodegradation: Involved Microorganisms and Genetically Engineered Microorganisms
N. T. Joutey (2013)
Use of microorganisms in the removal of pollutants from the wastewater
Giovanni Colica (2009)
Interference of chromium with biological systems in yeasts and fungi: a review
B. Poljsak (2010)
3-level Box–Behnkenoptimization of hexavalent chromium reduction by chromate resistant Trichoderma asperellum cells from simulated and industrial effluent
N. Saranya (2020)
The chromate resistance phenotype of some yeast mutants correlates with a lower level of Cr(V)-species generated in the extra-cellular medium
H. Ksheminska (2010)
From classic methodologies to application of nanomaterials for soil remediation: an integrated view of methods for decontamination of toxic metal(oid)s
Lilian Rodrigues Rosa Souza (2020)
Interference of Chromium with Cellular Functions
B. Poljsak (2011)
Sustainable bioreduction of toxic levels of chromate in a denitrifying granular sludge reactor
G. Kiran Kumar Reddy (2017)
Management of chromium induced oxidative stress by marine Bacillus licheniformis
Calcium alginate as an eco-friendly supporting material for Baker’s yeast strain in chromium bioremediation
M. S. Mahmoud (2017)
The kinetic reduction of Cr(VI) by yeast Saccharomyces cerevisiae, Phaffia rhodozyma and their protoplasts.
J. Chwastowski (2013)
Toxicity and Bioremediation of Heavy Metals Contaminated Ecosystem from Tannery Wastewater: A Review
B. Igiri (2018)
Comparative transcriptomic analysis reveals novel insights into the response to Cr(VI) exposure in Cr(VI) tolerant ectomycorrhizal fungi Pisolithus sp. 1 LS-2017.
L. Shi (2019)
Mechanisms of hexavalent chromium resistance and removal by microorganisms.
N. T. Joutey (2015)
Biotransformation of hexavalent chromium into extracellular chromium(III) oxide nanoparticles using Schwanniomyces occidentalis
Pallavi Mohite (2015)
Remediation techniques for removal of heavy metals from the soil contaminated through different sources: a review
S. S. Dhaliwal (2019)
Cr(VI) reduction by gluconolactone and hydrogen peroxide, the reaction products of fungal glucose oxidase: Cooperative interaction with organic acids in the biotransformation of Cr(VI).
P. Romo-Rodríguez (2015)
Bioreduction of chromium (VI) to chromium (III) by a novel yeast strain Rhodotorula mucilaginosa (MTCC 9315)
S. Chatterjee (2012)
Inducible chromate reductase exhibiting extracellular activity in Bacillus methylotrophicus for chromium bioremediation.
John Geraldine Sandana Mala (2015)
Microbial Ecology of Hydrocarbon Degradation in the Soil: A Review
P. Pandey (2016)
Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b₂.
O. Smutok (2011)
Increased copper bioremediation ability of new transgenic and adapted Saccharomyces cerevisiae strains
Polina Geva (2016)
Chromate-resistant mutants of the yeast Pichia guilliermondii: Selection and properties
H. Ksheminska (2011)
Effective Role of Microorganism in Waste Management and Environmental Sustainability
S. Mondal (2019)
Metallophilic fungi research: an alternative for its use in the bioremediation of hexavalent chromium
M. García-Hernández (2017)
Reduction of Cr ( VI ) by Bacillus megaterium Isolated from Sewage Treatment Plant
Karthika Velusamy (2017)
Chapter 11 Biodegradation : Involved Microorganisms and Genetically Engineered Microorganisms
N. T. Joutey (2013)
Yeast Tolerance to Chromium Depends on Extracellular Chromate Reduction and Cr(III) Chelation
Halyna Ksheminska (2008)
Role of Chromium Enriched Tobacco in the Occurrence of Oral Carcinogenesis
S. Samal (2020)See more