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Improved DPPH Determination For Antioxidant Activity Spectrophotometric Assay

M. Szabo, C. Idiţoiu, D. Chambre, A. Lupea
Published 2007 · Chemistry

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An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.
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