Online citations, reference lists, and bibliographies.
← Back to Search

The Cytotoxicity Of Dextran-coated Iron Oxide Nanoparticles On Hela And MCF-7 Cancerous Cell Lines

M. Rezaei, Hossein mafakheri, K. Khoshgard, A. R. Montazerabadi, Ahmad Mohammadbeigi, F. Oubari
Published 2017 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
Background: Recently, iron oxide nanoparticles have attracted attention in various diagnosis and treatment fields. The aim of the present study was to investigate the cytotoxicity of various concentrations and incubation times of dextran-coated iron oxide nanoparticles (DIONPs) on HeLa and MCF-7 cancerous cell lines. Methods: This in-vitro study was conducted at Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran in 2016. The dextran-coated iron oxide nanoparticles (DIONPs) uptake and cytotoxicity at different concentrations (10, 40 and 80 µg/ml) and different incubation times (6, 12 and 24 h) were assessed on HeLa and MCF-7 cell lines. The viability of the cells was measured by MTT assay. Results: DIONPs entered into the HeLa and MCF-7 cells. After 6, 12 and 24 h incubation times and in all concentrations, the viability of HeLa cells was more than 94. For MCF-7 cell line, increasing incubation time from 6 to 24 h at a concentration of 10 μg/ml decreased the cells viability from 98 to 95. When the cells were exposed to concentrations of 40 and 80 μg/ml of the nanoparticles, significant reductions in the cells viability was observed from 98 to 91.6 and from 95 to 88, respectively. Conclusion: DIONPs cytotoxicity increased by increasing the incubation time from 6 to 24 h and also increased with increasing the nanoparticles concentration from 0 to 80 μg/ml. In general, DIONPs did not cause considerable toxicity in both cell lines especially at lower concentrations. Therefore, these nanoparticles are good candidates for use in biomedical and cancer research studies.
This paper references
Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay
Homa Mohseni Kouchesfehani (2013)
10.1186/1477-3155-10-15
The cytotoxicity of polycationic iron oxide nanoparticles: Common endpoint assays and alternative approaches for improved understanding of cellular response mechanism
C. Hoskins (2012)
10.1002/ijc.21672
In vivo imaging of tumor response to therapy using a dual‐modality imaging strategy
Z. Medarova (2006)
10.1093/TOXSCI/KFJ027
Toxicity and tissue distribution of magnetic nanoparticles in mice.
J. Kim (2006)
10.1007/s11671-008-9239-9
Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging
Yuqing Ge (2009)
10.1002/smll.200700824
Synthesis of biocompatible dextran-coated nanoceria with pH-dependent antioxidant properties.
J. M. Perez (2008)
10.1021/nn203735b
Multidentate catechol-based polyethylene glycol oligomers provide enhanced stability and biocompatibility to iron oxide nanoparticles.
Hyon Bin Na (2012)
10.1021/ac701969u
Dextran-coated gold nanoparticles for the assessment of antimicrobial susceptibility.
S. Nath (2008)
10.1016/J.BIOMATERIALS.2004.10.012
Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications.
A. Gupta (2005)
10.1016/j.addr.2009.03.009
Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy.
P. Aggarwal (2009)
10.1002/ADEM.200990035
Cytotoxicity and Cell Cycle Effects of Bare and Poly(vinyl alcohol)‐Coated Iron Oxide Nanoparticles in Mouse Fibroblasts
M. Mahmoudi (2009)
10.1080/10934520600966177
Toxicity of Metal Oxide Nanoparticles in Mammalian Cells
H. Jeng (2006)
10.1002/smll.200901158
Effect of surface properties on nanoparticle-cell interactions.
Ayush Verma (2010)
10.1002/smll.201201531
Applications and potential toxicity of magnetic iron oxide nanoparticles.
G. Liu (2013)
10.1016/j.toxlet.2009.03.014
Size-dependent toxicity of metal oxide particles--a comparison between nano- and micrometer size.
H. Karlsson (2009)
10.1016/j.colsurfb.2014.06.064
Synthesis, characterization and toxicological evaluation of iron oxide nanoparticles in human lung alveolar epithelial cells.
S. Dwivedi (2014)
10.1088/0957-4484/20/11/115103
The influence of surface functionalization on the enhanced internalization of magnetic nanoparticles in cancer cells.
Ángeles Villanueva (2009)
10.1021/ES051043O
Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations.
Ludwig K. Limbach (2005)
10.1371/journal.pone.0123159
Glucose-Coated Superparamagnetic Iron Oxide Nanoparticles Prepared by Metal Vapour Synthesis Are Electively Internalized in a Pancreatic Adenocarcinoma Cell Line Expressing GLUT1 Transporter
D. Barbaro (2015)
10.1002/jbm.b.33178
Cellular internalization and detailed toxicity analysis of protein-immobilized iron oxide nanoparticles.
Purva Sanganeria (2015)
10.1021/mp7001285
Biodistribution, clearance, and biocompatibility of iron oxide magnetic nanoparticles in rats.
Tapan Jain (2008)
10.1088/0957-4484/21/7/075102
Biocompatibility of Fe(3)O(4) nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells.
B. Ankamwar (2010)
10.1021/tx800064j
Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes.
H. Karlsson (2008)
10.3109/09553002.2014.888104
The role of iron oxide nanoparticles in the radiosensitization of human prostate carcinoma cell line DU145 at megavoltage radiation energies
S. Khoei (2014)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar