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Inhibition Of Yeast Phagocytosis And Cell Spreading By Glucocorticoids In Cultures Of Resident Murine Peritoneal Macrophages.

R. Grasso, T. Klein, W. Benjamin
Published 1981 · Biology, Medicine

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This study was initiated to determine whether the inhibition of phagocytosis and cell spreading in cortisol-treated cultures of resident murine peritoneal macrophages are glucocorticoid-directed responses. Phagocytosis of heat-killed Saccharomyces cerevisiae and cell spreading were measured in control and steroid-treated macrophage cultures over 6 days. When the cultures were exposed to testosterone, progesterone, or epicortisol, phagocytosis and cell spreading were similar to controls. In contrast, both macrophage functions were inhibited significantly in cultures treated with cortisol, methylprednisolone, dexamethasone, and triamcinolone acetonide. In addition, the rate of phagocytosis was retarded and phagocytic indices (i.e., yeast particle number/cell) were reduced in glucocorticoid-treated cultures. Dose-response studies with dexamethasone demonstrated that the ED50 for the inhibitory effect on phagocytosis was 20 nM. These results indicate that the inhibition of yeast phagocytosis and cell spreading in the steroid-treated cultures are specific glucocorticoid-directed responses.
This paper references
10.1084/JEM.137.4.1031
GLUCOCORTICOID SUPPRESSION OF MACROPHAGE MIGRATION INHIBITORY FACTOR
J. Balow (1973)
10.1016/0006-2952(75)90181-1
Effects of anti-inflammatory and other compounds on the release of lysosomal enzymes from macrophages.
P. Ringrose (1975)
The in vitro effect of hydrocortisone on the uptake and intracellular digestion of particulate matter by macrophages in culture.
E. Wiener (1972)
10.3109/08923977809027327
Mechanisms of the immunosuppressive and anti-inflammatory effects of glucocorticosteroids.
A. Fauci (1978)
10.1146/ANNUREV.PA.19.040179.001143
Mechanisms of glucocorticoid action on immune processes.
J. Parrillo (1979)
In vitro actions of glucocorticoids on murine macrophages: effects on glucose transport and metabolism, growth in culture, and protein synthesis.
J. Norton (1980)
10.1016/0008-8749(76)90329-4
Suppression of macrophage arming by corticosteroids.
A. Dimitriu (1976)
10.1016/0006-2952(79)90555-0
Effects of anti-inflammatory and immuno-modulating agents on the release of β-glucuronidase and collagenase from cultured macrophages of guinea pigs
Okimura Tsutomu (1979)
10.1084/JEM.147.6.1684
Interaction of glucocorticoids with macrophages. Identification of glucocorticoid receptors in monocytes and macrophages
Z. Werb (1978)
10.1084/JEM.125.5.807
COMPARATIVE EFFECTS OF CORTICOSTEROIDS ON HOST RESISTANCE TO INFECTION IN RELATION TO CHEMICAL STRUCTURE
R. Fauve (1967)
10.1084/JEM.121.1.153
THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY
Z. Cohn (1965)
10.1128/IAI.12.4.699-705.1975
Effect of glucocorticosteroids on the phagocytosis and intracellular killing by peritoneal macrophages.
T. L. Zwet (1975)
The in vitro effect of hydrocortisone on cultures of peritoneal monocytes.
E. Wiener (1969)
10.1084/JEM.144.6.1531
The induction of macrophage spreading: role of coagulation factors and the complement system
C. Bianco (1976)
10.1016/0092-8674(76)90011-8
Macrophage plasminogen activator: Modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides
J. Vassalli (1976)
10.1084/JEM.147.6.1695
Biochemical actions of glucocorticoids on macrophages in culture. Specific inhibition of elastase, collagenase, and plasminogen activator secretion and effects on other metabolic functions
Z. Werb (1978)



This paper is referenced by
10.1002/jlb.45.6.503
Characteristics of Monocyte Angiotensin‐Converting Enzyme (ACE) Induction by Dexamethasone
Z. Vuk-Pavlović (1989)
10.1016/0022-1759(86)90095-5
A rapid radiometric assay for measuring phagocytosis of Saccharomyces cerevisiae in macrophage cultures.
J. Becker (1986)
10.1016/S0006-291X(88)81135-5
Dexamethasone action inhibits the release of arachidonic acid from phosphatidylcholine during the suppression of yeast phagocytosis in macrophage cultures.
J. Becker (1988)
10.1002/jlb.36.6.771
Inhibition of Yeast Phagocytosis in Macrophage Cultures Treated With Slime Polysaccharide Purified From Pseudomonas aeruginosa
R. Grasso (1984)
10.1016/0192-0561(88)90118-X
Suppression of yeast ingestion by dexamethasone in macrophage cultures: evidence for a steroid-induced phagocytosis inhibitory protein.
J. Becker (1988)
10.1002/jlb.39.6.679
Tetrahydrocannabinol‐Induced Suppression of Macrophage Spreading and Phagocytic Activity In Vitro
M. Lopez-Cepero (1986)
Murine Macrophage Morphology Calcium Modulation of Phorbol Ester-induced Alterations in Updated Version
B. M. Smith (2006)
10.1002/JCP.1041240326
Opposing effects of dexamethasone on the clonal growth of granulocyte and macrophage progenitor cells and on the phagocytic capability of mononuclear phagocytes at different stages of differentiation
E. Shezen (1985)
10.1007/BF01741724
Selective restoration of immunosuppressive effect of cytotoxic agents by thymopoietin fragments
László Dénes (2005)
10.1067/MSY.2002.122376
Effects of cortisol administration on hepatic circulation during brain death in rabbits.
K. Ogawa (2002)
Tetrahydrocannabi fbi-Induced Suppressibn bf Macrbphage Spreading and Phagbcytic Activity In Vitrb
M. Lopez-Cepero (1986)
16-Epiestriol, a Novel Anti-Inflammatory Nonglycogenic Steroid, Does Not Inhibit IFN-y Production by
Edwin S. Miller (1998)
10.1016/S0165-2427(00)00186-0
Non-specific responses of turbot (Scophthalmus maximus L.) adherent cells to microsporidian spores.
J. Leiro (2000)
10.1016/0041-008X(84)90041-3
Evaluation of immunomodulatory chemicals: alteration of macrophage function in vitro.
P. Tam (1984)
10.1136/thx.45.5.362
Effects of an inhaled corticosteroid, budesonide, on alveolar macrophage function in smokers.
H. Bergstrand (1990)
10.1016/0022-1759(89)90230-5
An improved spectrofluorometric assay for quantitating yeast phagocytosis in cultures of murine peritoneal macrophages.
R. Ragsdale (1989)
10.1016/0192-0561(85)90046-3
Suppression of phagocytosis by dexamethasone in macrophage cultures: inability of arachidonic acid, indomethacin, and nordihydroguaiaretic acid to reverse the inhibitory response mediated by a steroid-inducible factor.
J. Becker (1985)
10.1016/j.steroids.2008.12.013
Genomic and non-genomic effect of cortisol on phagocytosis in freshwater teleost Channa punctatus: An in vitro study
B. Roy (2009)
10.1007/978-1-4757-1410-4_23
A feasibility study to determine if microbicidal activity can be measured in dexamethasone-treated macrophage cultures.
R. Grasso (1983)
10.1007/978-1-4613-2253-5_24
Transient Suppression of Yeast Phagocytosis Induced by Legionella Pneumophila in Cultures of Murine Resident Peritoneal Macrophages
J. Becker (1986)
10.1016/j.ygcen.2010.11.021
Immunomodulatory role of urotensins in teleost Channa punctatus.
R. Singh (2011)
10.1016/j.yfrne.2013.09.005
Why do we need nongenomic glucocorticoid mechanisms?
C. Jiang (2014)
This information is current as Phagocytosis of Apoptotic Leukocytes Glucocorticoids Promote Nonphlogistic
Y. Liu (1999)
10.1080/09273948.2016.1245758
A Review of the Role of Intravitreal Corticosteroids as an Adjuvant to Antibiotics in Infectious Endophthalmitis
Dawn Ching Wen Ho (2018)
10.1016/S0168-0102(01)00231-0
Response of amoeboid and differentiating ramified microglia to glucocorticoids in postnatal rats: a lectin histochemical and ultrastructural study
C. Wu (2001)
10.1158/1940-6207.CAPR-14-0380
Improved Innate Immune Responses by Frondanol A5, a Sea Cucumber Extract, Prevent Intestinal Tumorigenesis
N. Janakiram (2015)
Glucocorticoids promote nonphlogistic phagocytosis of apoptotic leukocytes.
Y. Liu (1999)
10.3748/WJG.V12.I41.6678
Arterial steroid injection therapy can inhibit the progression of severe acute hepatic failure toward fulminant liver failure.
K. Kotoh (2006)
10.1016/j.steroids.2004.10.004
Rapid nongenomic inhibitory effects of glucocorticoids on phagocytosis and superoxide anion production by macrophages
F. Long (2005)
10.1258/ar.2011.110373
Radiological catheter placement for transcatheter arterial steroid injection therapy to treat severe acute hepatic failure: technical feasibility and efficacy
Y. Ushijima (2012)
10.3748/wjg.v17.i44.4928
Acute liver failure caused by drug-induced hypersensitivity syndrome associated with hyperferritinemia.
M. Miyazaki (2011)
10.1002/jlb.47.1.79
Control of Lipoprotein Lipase Secretion by Macrophages: Effect of Macrophage Differentiation Agents
R. Goldman (1990)
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