Online citations, reference lists, and bibliographies.
← Back to Search

Cytocidal Mechanism Of TNF: Effects Of Lysosomal Enzyme And Hydroxyl Radical Inhibitors On Cytotoxicity.

N. Watanabe, Y. Niitsu, H. Neda, H. Sone, N. Yamauchi, M. Maeda, I. Urushizaki
Published 1988 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
The participation of lysosomal enzymes, hydroxyl radicals, and mitochondrial respiration in the cytocidal effect of TNF on tumor cells was investigated. The cytotoxicity of TNF on L-M cells was clearly reduced by lysosomotropic agents, DMSO (hydroxyl radical scavenger), NDGA (lipoxygenase inhibitor), and sodium azide (mitochondrial respiration inhibitor). The results suggest that lysosomal enzyme and hydroxyl radicals play an important triggering role in the destruction of tumor cells by TNF, and that the process of destruction might require ATP.
This paper references
Tumor necrosis factor: specific binding and internalization in sensitive and resistant cells.
M. Tsujimoto (1985)
Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor
T. Shirai (1985)
Mechanism of the cytotoxic effect of tumor necrosis factor.
Y. Niitsu (1985)
Effects of indomethacin, 5,8,11,14-eicosatetraynoic acid, and p-bromophenacyl bromide on lysosomal enzyme release and superoxide anion generation by human polymorphonuclear leukocytes.
J. Smolen (1980)
Superoxide dismutase activity of Ehrlich ascites tumor cells.
S. K. Sahu (1977)
Tumour-necrosis factor from the rabbit. I. Mode of action, specificity and physicochemical properties.
N. Matthews (1978)
Effect of tumour necrosis factor on cultured human melanoma cells
L. Helson (1975)
[Anti-tumor effect and the mechanism of action of human recombinant TNF].
N. Watanabe (1986)
An endotoxin-induced serum factor that causes necrosis of tumors.
E. Carswell (1975)
Possible requirement of internalization in the mechanism of in vitro cytotoxicity in tumor necrosis serum.
F. Kull (1981)
T cell involvement in production of tumor necrosis factor: reconstitution experiments with nude mice.
Y. Niitsu (1985)
Antitumor effect of tumor necrosis factor against various primarily cultured human cancer cells.
N. Watanabe (1985)
Evidence that peroxidation of lysosomal membranes is initiated by hydroxyl free radicals produced during flavin enzyme activity.
K. L. Fong (1973)

This paper is referenced by
Potent inhibition of interleukin 1 beta-mediated human melanoma (A375.6) lysis by corticosteroids, staurosporine, and tilorone.
R. Schultz (1989)
Oxidative damage in murine tumor cells treated in vitro by recombinant human tumor necrosis factor.
R. J. Zimmerman (1989)
Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor
G. Wong (1989)
TNF accelerates the S‐phase of the cell cycle in tumor cells
S. Volland (1994)
Glucose depletion enhances the anti‐tumor effect of TNF
S. Volland (1992)
Comparison between tumour necrosis factor response and heat shock response in L929 cells: Cellular and molecular aspects
Y. Lee (1992)
N-acetylcysteine and glutathione as inhibitors of tumor necrosis factor production.
P. Peristeris (1992)
Links between Innate Immunity and Normal Tissue Radiobiology
D. Schaue (2010)
Radiation takes its Toll.
Josephine A. Ratikan (2015)
Suppressive effects of intracellular glutathione on hydroxyl radical production induced by tumor necrosis factor
N. Yamauchi (1990)
Lysosome Labilizers Potentiate the Antitumor Effects of Tumor Necrosis Factor‐α
T. Masegi (1993)
The molecular action of tumor necrosis factor‐α
G. Camussi (1991)
Reduced expression of manganese superoxide dismutase in cells resistant to cytolysis by tumor necrosis factor.
J. Melendez (1992)
Effects of n−3 and n−6 fatty acids on tumor necrosis factor cytotoxicity in WEHI fibrosarcoma cells
O. Brekke (2006)
Spin trap protection from tumor necrosis factor cytotoxicity.
H. Pogrebniak (1991)
Specificity of hydroperoxy fatty acid inhibition of cell growth and the lack of effect on tumour necrosis factor-induced cytotoxicity in WEHI clone 13 cells.
R. Nøding (1997)
Cytokines in Radiobiological Responses: A Review
D. Schaue (2012)
New insights into tumor necrosis factor-alpha in cancer: Distinct isoforms exert opposing effects on tumor associated myeloid cells and tumorigenesis
S. Ardestani (2013)
Expression of Tumor Necrosis Factor-Alpha-Mediated Genes Predicts Recurrence-Free Survival in Lung Cancer
B. Wang (2014)
Inhibition of target cell mitochondrial electron transfer by tumor necrosis factor
J. Lancaster (1989)
Tumor necrosis factor activities and cancer therapy--a perspective.
R. S. Sidhu (1993)
Intracellular hydroxyl radical production induced by recombinant human tumor necrosis factor and its implication in the killing of tumor cells in vitro.
N. Yamauchi (1989)
The sensitivity of head and neck squamous cell carcinomas to tumor necrosis factor-α
I. Mochimatsu (1993)
Expression and DNA methylation of TNF, IFNG and FOXP3 in colorectal cancer and their prognostic significance
S. Ganapathi (2014)
Semantic Scholar Logo Some data provided by SemanticScholar