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Adhesion Of Positively Charged Liposomes To Mucosal Tissues
L. S. Guo, R. Radhakrishnan, C. Redemann
Published 1989 · Chemistry
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AbstractA series of positively charged phospholipid and cholesterol derivatives was synthesized and evaluated as membrane components for liposomes. Small unilamellar liposomes containing up to 40 mole% of the synthetic lipids were prepared by sonication. Selected liposome preparations containing these synthetic lipid materials were found to be noncytotoxic in vitro by using a cell growth inhibition assay, whereas liposomes containing more classic positively charged components (stearylamine and cetyltrimethylammonium bromide) showed considerable cytotoxicity. Using an unanesthetized rabbit eye model, we have found that inclusion of the positively charged lipid derivatives into the liposomes significantly enhanced the ocular retention compared to neutral or negatively charged liposomes, presumably by molecular association with poly anionic corneal and conjunctival surface mucoglycoproteins. the increased retention was dependent on charge density and rigidity of the lipid bilayer. An assay for primary amino ...
This paper references
Nasal absorption of insulin: enhancement by hydrophobic bile salts.
G. Gordon (1985)
Selective intraocular delivery of liposome encapsulated inulin via the non-comeal absorption route
I. Ahmed (1986)
Wettability and wetting of corneal epithelium.
F. Holly (1971)
Phosphatidylcholine vesicles. Formation and physical characteristics
C. Huang (1969)
Liposomes in topical drug delivery.
H. E. Schaeffer (1982)
Treatment of hepatosplenic candidiasis with liposomal-amphotericin B.
G. López-Berestein (1987)
Characterization, toxicity and therapeutic efficacy of adriamycin encapsulated in liposomes.
F. Olson (1982)
Interaction of unilamellar liposomes with serum lipoproteins and apolipoproteins.
L. S. Guo (1980)
Intranasal aerosolized insulin. Mixed-meal studies and long-term use in type I diabetes.
R. Salzman (1985)
Phosphorus assay in column chromatography.
G. R. Bartlett (1959)
The effects of ophthalmic drugs, vehicles, and preservatives on corneal epithelium: a scanning electron microscope study.
R. Pfister (1976)
Liposomes and tear fluid. I. Release of vesicle-entrapped carboxyfluorescein.
R. F. Barber (1986)
Reduction in doxorubicin toxicity following liposomal delivery.
R. Sells (1987)
Liposomes are effective carriers for the ocular delivery of prophylactics.
P. Shek (1987)
A rapid method of total lipid extraction and purification.
E. Bligh (1959)
Idoxuridine-liposome therapy for herpes simplex keratitis.
G. Smolin (1981)
Ocular distribution of liposome-encapsulated epinephrine and inulin in the albino rabbit.
R. Stratford (1982)
Fractionation and chemical properties of tear mucoids.
S. Iwata (1971)
The use of a new radioactive-iodine labeled lipid marker to follow in vivo disposition of liposomes: comparison with an encapsulated aqueous space marker.
R. M. Abra (1982)
Liposome toxicity in the mouse central nervous system
D. H. Adams (1977)
Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes.
F. Olson (1979)
The influence of non-ionic detergents and other surfactants on human corneal permeability.
R. Marsh (1971)
Ophthalmic drug delivery systems
J. Shell (1984)
Demonstration of the mucous layer of the tear film by electron microscopy.
B. Nichols (1985)
Solubilisation of phospholipid membranes by human plasma high density lipoproteins
A. Tall (1977)
Precorneal factors influencing the ocular distribution of topically applied liposomal inulin.
V. H. Lee (1984)
Statistical analysis for experimental models of ocular disease: continuous response measures.
W. Ray (1985)
Transbilayer exchange of phosphatidylethanolamine for phosphatidylcholine and N-acetimidoylphosphatidylethanolamine in single-walled bilayer vesicles.
M. Roseman (1975)
A gamma scintigraphic evaluation of the precorneal residence of liposomal formulations in the rabbit
P. Fitzgerald (1987)
Ocular drug bioavailability from topically applied liposomes.
V. H. Lee (1985)
Liposomes as Drug Carriers for Oral Ulcers
B. B. Harsanyi (1986)
Liposomal amphotericin B for the treatment of systemic fungal infections in patients with cancer: a preliminary study.
G. López-Berestein (1985)
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Positively charged nanoparticles for improving the oral bioavailability of cyclosporin-A.
M. H. El-Shabouri (2002)
Enhanced gastrointestinal absorption of N3-O-toluyl-fluorouracil by cationic solid lipid nanoparticles
Donghua Liu (2010)
Prolonged transgene expression in rodent lung cells.
R. Debs (1992)
The use of bioadhesives in targeted delivery within the gastrointestinal tract
M. Helliwell (1993)
Preparation and evaluation of acetazolamide liposomes as an ocular delivery system
O. El-Gazayerly (1997)
Pulsatile insulin delivery through the ocular route.
V. Soni (1998)
Design, characterization and preclinical efficacy of a cationic lipid adjuvant for influenza split vaccine.
B. Guy (2001)
ENHANCEMENT OF NASAL ABSORPTION OF CALCITONIN LOADED IN LIPOSOMES
S. Law (2001)
Liposomes in Drug Delivery
G. Gregoriadis (2012)
Evaluation of cationic polymer-coated nanocapsules as ocular drug carriers
P. Calvo (1997)
Liposomal Drug Delivery
R. Fielding (1991)
The Role of Surface Active Agents in Ophthalmic Drug Delivery: A Comprehensive Review.
Shaimaa S Ibrahim (2019)
Probing influence of methodological variation on active loading of acetazolamide into nanoliposomes: biophysical, in vitro, ex vivo, in vivo and rheological investigation.
Prashant B Pisal (2014)
Membrane peroxidation: inhibiting effects of water-soluble antioxidants on phospholipids of different charge types.
L. Barclay (1994)
Cationic Liposomes Containing Noncytotoxic Phospholipid and Cholesterol Derivatives
L. S. Guo (1993)
Nonviral Gene Delivery
F. Sorgi (1999)
Chitosan-coated lipid nanocarriers for therapeutic applications
F. Oyarzun-Ampuero (2010)
Development of positively charged colloidal drug carriers: Chitosan-coated polyester nanocapsules and submicron-emulsions
P. Calvo (1997)
Mucoadhesion: A promising approach in drug delivery system
Shakir Mansuri (2016)
Development and characterization of Cyclosporine A loaded nanoparticles for ocular drug delivery: Cellular toxicity, uptake, and kinetic studies.
P. Aksungur (2011)
Acyclovir-containing liposomes for potential ocular delivery. Corneal penetration and absorption.
S. Law (2000)
The extended ocular hypotensive effect of positive liposomal cholesterol bound timolol maleate in glaucomatous rabbits
M. Shafaa (2011)