Online citations, reference lists, and bibliographies.

Adipose-derived Stem Cells: Characterization And Current Application In Orthopaedic Tissue Repair.

Hazel Tapp, Edward N. Hanley, Joshua C. Patt, Helen Elizabeth Gruber
Published 2009 · Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
Orthopaedic tissues, such as bone, cartilage, intervertebral disc and tendon, contain cells that are difficult to culture and stimulate in vitro for repair of damaged tissue. Stem cells have the ability to self-renew and differentiate into many tissue types. Recent progress in stem cell research has led to an enthusiastic effort to utilize stem cells for orthopaedic tissue regeneration. Due to ease of harvest and abundance, adipose-derived mesenchymal cells (ASC) are an attractive, readily available adult stem cell that has become increasingly popular for use in many stem cell applications. Recent progress has been made in characterizing ASC and looking mechanistically at gene expression and cellular pathways involved in differentiation. This review focuses on (i) the characterization of ASC through expression of appropriate surface markers; (ii) modulation of in vitro differentiation of ASC through different scaffolds, growth factors, and media; and (iii) the use of ASC in orthopaedic tissue repair. Strategies for repair involve the use of differentiated or undifferentiated, fresh or passaged ASC, in conjunction with appropriate choice of media, growth factors and scaffolds. Recent in vivo studies utilizing ASC are discussed giving results on defect repair and potential for clinical orthopaedic tissue regeneration.
This paper references
10.1016/j.bbrc.2007.06.180
Human adipose tissue-derived multipotent stem cells differentiate in vitro and in vivo into osteocyte-like cells.
Christian Elabd (2007)
10.1002/jcp.20238
FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells.
Luis A. Solchaga (2005)
10.1111/j.1582-4934.2008.00278.x
Influence of collagen type II and nucleus pulposus cells on aggregation and differentiation of adipose tissue-derived stem cells
ZuFu Lu (2008)
10.1016/s1535-1513(08)79155-0
Collagenous Microbeads as a Scaffold for Tissue Engineering with Adipose-Derived Stem Cells
Samuel H. Miller (2009)
10.1038/cr.2008.257
Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo
Yunfeng Lin (2008)
10.1242/jcs.02932
Mesenchymal stem cells reside in virtually all post-natal organs and tissues
Lindolfo da Silva Meirelles (2006)
10.1002/(SICI)1097-4636(19980905)41:3<412::AID-JBM11>3.0.CO;2-K
Cellular ingrowth and thickness changes in poly-L-lactide and polyglycolide matrices implanted subcutaneously in the rat.
Walter D. Holder (1998)
10.1089/107632701300062859
Multilineage cells from human adipose tissue: implications for cell-based therapies.
Patricia A. Zuk (2001)
10.1007/s10856-006-0693-6
Tissue engineered intervertebral disc repair in the pig using injectable polymers
Peter A. Revell (2007)
10.1089/ten.2006.12.2853
Differential expression pattern of extracellular matrix molecules during chondrogenesis of mesenchymal stem cells from bone marrow and adipose tissue.
Alexander T. Mehlhorn (2006)
10.1172/JCI22689
Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells.
Andrea Hoffmann (2006)
10.1007/s10616-007-9092-1
FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-β signaling
Tomomi Ito (2007)
10.1016/j.exphem.2005.07.003
Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood.
Wolfgang Wagner (2005)
Periodontal tissue regeneration with adipose-derived stem cells
飛田 護邦 (2010)
10.1091/mbc.E02-02-0105
Human adipose tissue is a source of multipotent stem cells.
Patricia A. Zuk (2002)
10.1006/bbrc.2001.6270
Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.
Geoffrey R. Erickson (2002)
10.1002/jcp.1138
Surface protein characterization of human adipose tissue-derived stromal cells.
Stan Gronthos (2001)
10.1089/ten.2006.0102
In vivo osteogenic potential of human adipose-derived stem cells/poly lactide-co-glycolic acid constructs for bone regeneration in a rat critical-sized calvarial defect model.
Eulsik Yoon (2007)
10.1634/stemcells.2006-0394
Identification of common pathways mediating differentiation of bone marrow- and adipose tissue-derived human mesenchymal stem cells into three mesenchymal lineages.
Tong Ming Liu (2006)
10.1016/j.bbrc.2007.06.002
Differentiation of adipose stem cells by nucleus pulposus cells: configuration effect.
ZuFu Lu (2007)
10.1080/14653240500319093
Anterior cruciate ligament constructs fabricated from human mesenchymal stem cells in a collagen type I hydrogel.
Ulrich Noeth (2005)
10.1089/ten.2006.0165
Stem cells from adipose tissue allow challenging new concepts for regenerative medicine.
Marco N. Helder (2007)
10.1002/jcp.20977
Reduced chondrogenic potential of adipose tissue derived stromal cells correlates with an altered TGFbeta receptor and BMP profile and is overcome by BMP-6.
Thea Hennig (2007)
10.1016/j.yexcr.2005.04.029
Functional studies of mesenchymal stem cells derived from adult human adipose tissue.
Andrea Dicker (2005)
10.1089/scd.2006.0026
Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells.
Maikel J Oedayrajsingh Varma (2007)
10.1159/000081088
Osteogenic Potential of Human Adipose Tissue-Derived Stromal Cells as an Alternative Stem Cell Source
Hidemi Hattori (2004)
10.1634/stemcells.2005-0234
Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers.
James B. Mitchell (2006)
10.1002/art.21779
Potent induction of chondrocytic differentiation of human adipose-derived adult stem cells by bone morphogenetic protein 6.
Bradley T. Estes (2006)
10.1089/ten.2005.11.120
Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue.
Brett Peterson (2005)
10.1302/0301-620x.88b7.17305
The potential of stem cells in orthopaedic surgery.
Eunjoo H. Lee (2006)
10.1089/107632704323061735
Human adipose-derived adult stem cells produce osteoid in vivo.
Kevin C Hicok (2004)
10.1634/stemcells.2006-0228
Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease.
Rosa Yañez (2006)
10.1002/jcp.1041610209
Proliferation and differentiation of rat adipose precursor cells in chemically defined medium: differential action of anti-adipogenic agents.
Georges Vassaux (1994)
10.1080/14653240600987690
Regulation of adipose-derived adult stem cells differentiating into chondrocytes with the use of rhBMP-2.
Yiyong Wei (2006)
10.1007/s11154-006-9001-5
Networks and hubs for the transcriptional control of osteoblastogenesis
Jane B Lian (2006)
10.1007/s00167-007-0385-x
ACL reconstruction in a rabbit model using irradiated Achilles allograft seeded with mesenchymal stem cells or PDGF-B gene-transfected mesenchymal stem cells
Feng lin Li (2007)
10.1634/stemcells.2004-0062
Directing stem cell differentiation into the chondrogenic lineage in vitro.
Boon Chin Heng (2004)
10.1002/jcb.20284
Wnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cells.
Genevieve M Boland (2004)
10.1126/science.284.5411.143
Multilineage potential of adult human mesenchymal stem cells.
Mark F. Pittenger (1999)
10.1016/S0076-6879(06)20018-1
Engineering three-dimensional tissue structures using stem cells.
Janet Zoldan (2006)
10.1002/jbm.a.30686
Adhesion of mesenchymal stem cells to polymer scaffolds occurs via distinct ECM ligands and controls their osteogenic differentiation.
Sara R. Chastain (2006)
10.1097/01.prs.0000221037.34883.0a
A Novel Perfluoroelastomer Seeded with Adipose-Derived Stem Cells for Soft-Tissue Repair
Julio A. Clavijo-Alvarez (2006)
10.1016/j.bbrc.2007.05.098
Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system.
Yue Xu (2007)
10.1097/00006534-200203000-00037
Rat extramedullary adipose tissue as a source of osteochondrogenic progenitor cells.
Jerry I. Huang (2002)
10.1007/s00441-006-0308-z
Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle
Hideya Yoshimura (2006)
10.1080/14653240701679873
Rapid expansion of human adipose-derived stromal cells preserving multipotency.
Hiraku Suga (2007)
10.1016/j.tibtech.2007.07.009
Stem cells in veterinary medicine--attempts at regenerating equine tendon after injury.
Lucy E Richardson (2007)
10.1097/01.brs.0000184365.28481.e3
Differentiation of Mesenchymal Stem Cells Transplanted to a Rabbit Degenerative Disc Model: Potential and Limitations for Stem Cell Therapy in Disc Regeneration
Daisuke Sakai (2005)
10.1111/j.1365-2141.2005.05409.x
Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells.
Bénédicte Puissant (2005)
10.1080/14653240310003026
Adipose-derived adult stem cells: isolation, characterization, and differentiation potential.
Jm Gimble (2003)
Induction of a disc-like phenotype in adipose derived mesenchymal stem cells:Effects of TGF-beta3 and three-dimensional culture
H Tapp (2007)
10.1089/ten.2006.0249
Healing full-thickness cartilage defects using adipose-derived stem cells.
Jason L. Dragoo (2007)
10.1002/ar.a.10039
Mesenchymal stem cells from rat visceral fat exhibit multipotential differentiation in vitro.
Sunil S. Tholpady (2003)
10.1016/j.spinee.2007.09.011
Feasibility of a stem cell therapy for intervertebral disc degeneration.
Satoshi Sobajima (2008)
10.1007/s00418-005-0014-z
Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes
Franck Festy (2005)
10.1038/nbt958
Adipose-derived adult stromal cells heal critical-size mouse calvarial defects
Catherine M. Cowan (2004)
10.1186/ar2116
Mesenchymal stromal cells. Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation
Catherine M Kolf (2007)
10.1016/j.bbrc.2007.02.165
Bone regeneration by implantation of adipose-derived stromal cells expressing BMP-2.
Huiwu Li (2007)
10.1016/j.bbrc.2006.02.052
Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells.
Marlene Knippenberg (2006)
10.1080/14653240600621125
Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure.
Maikel J. Oedayrajsingh-Varma (2006)
10.4049/jimmunol.179.3.1595
Adult Human Fibroblasts Are Potent Immunoregulatory Cells and Functionally Equivalent to Mesenchymal Stem Cells1
Muzlifah Haniffa (2007)
10.2302/kjm.54.132
Multipotential differentiation of adipose tissue-derived stem cells.
Brian M. Strem (2005)
10.1634/stemcells.2006-0420
Derivation of clinically compliant MSCs from CD105+, CD24- differentiated human ESCs.
Qizhou Lian (2007)
10.1016/j.bbrc.2006.02.171
Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adipose-derived mesenchymal cells.
Michael Chiou (2006)
10.1016/j.biomaterials.2003.10.045
Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds.
Hani A Awad (2004)
10.1080/14653240600855905
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.
Massimiliano Dominici (2006)
10.1002/jbm.b.30357
Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold.
Hidemi Hattori (2006)
10.2106/JBJS.G.00292
Stem cells from human fat as cellular delivery vehicles in an athymic rat posterolateral spine fusion model.
Wellington K Hsu (2008)
10.1097/01.sap.0000210629.17727.bd
The Osteogenic Potential of Adipose-Derived Stem Cells for the Repair of Rabbit Calvarial Defects
Jason R. Dudas (2006)
10.1089/107632701753337681
Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells.
Y D Halvorsen (2001)
10.1634/stemcells.2004-0021
Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells.
Adam J. Katz (2005)
10.1016/j.jcms.2004.05.011
Injectable bone using chitosan-alginate gel/mesenchymal stem cells/BMP-2 composites.
Dong-Joon Park (2005)
10.1016/j.orthres.2005.03.008.1100230621
Chondrogenic potential of progenitor cells derived from human bone marrow and adipose tissue: a patient-matched comparison.
Jerry I. Huang (2005)
10.1016/j.matbio.2007.07.002
Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells.
Shyni Varghese (2008)



This paper is referenced by
10.3390/ijms18122597
Function of microRNAs in the Osteogenic Differentiation and Therapeutic Application of Adipose-Derived Stem Cells (ASCs)
Walter M Hodges (2017)
10.1002/jor.22439
Impact of direct cell co-cultures on human adipose-derived stromal cells and nucleus pulposus cells.
Zhen Sun (2013)
10.1186/s13287-016-0412-2
Localization of human adipose-derived stem cells and their effect in repair of diabetic foot ulcers in rats
Rongfeng Shi (2016)
10.6051/IJO.V2I5.1164
Tendon healing: Biological principles and augmentation with autogenous mesenchymal stem cells. Current concepts
Nikolaos Platon Sachinis (2015)
10.3892/ijmm.2012.1123
Comparative study of the osteogenic differentiation capacity of human bone marrow- and human adipose-derived stem cells under cyclic tensile stretch using quantitative analysis.
Yaping Ye (2012)
10.1016/j.biomaterials.2014.04.055
The epigenetic promotion of osteogenic differentiation of human adipose-derived stem cells by the genetic and chemical blockade of histone demethylase LSD1.
Wenshu Ge (2014)
10.5966/sctm.2012-0009
High plasticity of pediatric adipose tissue-derived stem cells: too much for selective skeletogenic differentiation?
Leonardo Guasti (2012)
EVALUATION OF THE OSTEOGENIC AND ENDOTHELIAL DIFFERENTIATION POTENTIAL OF SSEA-4 SUBPOPULATION OF ADIPOSE DERIVED STEM CELLS
Silvia M. Mihaila (2010)
10.18002/10612/898
Efecto de la implantación de células mesenquimales de tejido adiposo diferenciadas a osteoblastos en la reparación de defectos cavitarios del fémur en un modelo experimental ovino
Andrés Saldaña Díaz (2011)
10.1007/s11307-018-1218-7
Tumor Formation of Adult Stem Cell Transplants in Rodent Arthritic Joints
Fanny Chapelin (2018)
10.1159/000478751
Identification and Characterization of Long Non-Coding RNAs in Osteogenic Differentiation of Human Adipose-Derived Stem Cells
Guangxin Huang (2017)
10.1016/J.JEVS.2010.11.014
Use of Adipose Tissue-Derived Mesenchymal Stem Cells for Experimental Tendinitis Therapy in Equines
Armando de Mattos Carvalho (2011)
Response of Human Adipose Derived Stem Cels to 5-azacytidine and Zebularine Treatment , Culturing in Methylcelulose-based Medium and Matrix Elasticity Aalborg University 2009
Vladimir Zachar (2009)
10.1007/s11259-018-9738-9
Could hypoxia influence basic biological properties and ultrastructural features of adult canine mesenchymal stem /stromal cells?
Eleonora Iacono (2018)
10.1089/ten.TEC.2009.0487
Buccal fat pad, an oral access source of human adipose stem cells with potential for osteochondral tissue engineering: an in vitro study.
Elisabet Farré-Guasch (2010)
10.1089/cell.2012.0077
Effects of epidermal growth factor and basic fibroblast growth factor on the proliferation and osteogenic and neural differentiation of adipose-derived stem cells.
Feihu Hu (2013)
10.1016/j.wneu.2011.12.084
Transplantation of human adipose-derived stem cells in a rabbit model of traumatic degeneration of lumbar discs.
Hyoung-Joon Chun (2012)
10.1007/s11259-011-9492-8
Characterization of adipose-derived equine and canine mesenchymal stem cells after incubation in agarose-hydrogel
Christina A. Schwarz (2011)
10.1016/j.biomaterials.2009.05.001
PHB/PHBHHx scaffolds and human adipose-derived stem cells for cartilage tissue engineering.
Chuan Ye (2009)
InVitro Differentiation andMaturation of Human Embryonic StemCell intoMultipotent Cells
AmerMahmood (2014)
10.1111/cpr.12029
Effects of serum reduction and VEGF supplementation on angiogenic potential of human adipose stromal cells in vitro.
Kien-Hui Chua (2013)
10.3906/biy-1501-61
Enhancement of chondrogenic differentiation potential of equine adiposetissue-derived mesenchymal stem cells using TGF-ß3 and BMP-6
Milad Shademan (2016)
10.2147/IJN.S238266
Homing of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) Labeled Adipose-Derived Stem Cells by Magnetic Attraction in a Rat Model of Parkinson’s Disease
Ardeshir Moayeri (2020)
10.3390/cells9051246
Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens
Sung-ho Chang (2020)
10.1016/j.biomaterials.2013.06.018
Mutual effect of subcutaneously transplanted human adipose-derived stem cells and pancreatic islets within fibrin gel.
Suk Ho Bhang (2013)
10.1093/bmb/ldx030
Adipose-derived stem cells in orthopaedic pathologies
Federico Giuseppe Usuelli (2017)
10.1016/j.mehy.2012.12.032
A new choice for the treatment of segmental tibial bone defects: intramedullary nail internal fixation combined adipose-derived stem cells technique.
Guang-Wen Fang (2013)
10.1371/journal.pone.0073673
VEGF-Mediated Proliferation of Human Adipose Tissue-Derived Stem Cells
Guangfeng Chen (2013)
10.1007/s13770-013-1126-5
Fabrication and evaluation of growth factor-immobilized injectable microspheres for the soft tissue augmentation
Jun Goo Kang (2013)
10.1590/S0100-736X2017000600019
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood
Gabrielle Rosemblit Martins (2017)
KAISA VUORNOS DYNAMIC CULTURE OF HUMAN ADIPOSE STEM CELLS IN A FLOW PERFUSION BIOREACTOR
Suvi P. Haimi (2016)
10.2174/157421019941450888x
Tendon regeneration and repair with adipose derived stem cells.
Ahmet Cağri Uysal (2010)
See more
Semantic Scholar Logo Some data provided by SemanticScholar