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Equol Status And Changes In Fecal Microbiota In Menopausal Women Receiving Long-term Treatment For Menopause Symptoms With A Soy-isoflavone Concentrate

Lucía Guadamuro, S. Delgado, Begoña Redruello, A. Flórez, A. Suárez, P. Martínez-Camblor, B. Mayo
Published 2015 · Biology, Medicine

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The knowledge regarding the intestinal microbial types involved in isoflavone bioavailability and metabolism is still limited. The present work reports the influence of a treatment with isoflavones for 6 months on the fecal bacterial communities of 16 menopausal women, as determined by culturing and culture-independent microbial techniques. Changes in fecal communities were analyzed with respect to the women’s equol-producing phenotype. Compared to baseline, at 1 and 3 months the counts for all microbial populations in the feces of equol-producing women had increased strongly. In contrast, among the non-producers, the counts for all microbial populations at 1 month were similar to those at baseline, and decreased significantly by 3 and 6 months. Following isoflavone intake, major bands in the denaturing gradient gel electrophoresis (DGGE) profiles appeared and disappeared, suggesting important changes in majority populations. In some women, increases were seen in the intensity of specific DGGE bands corresponding to microorganisms known to be involved in the metabolism of dietary phytoestrogens (Lactonifactor longoviformis, Faecalibacterium prausnitzii, Bifidobacterium sp., Ruminococcus sp.). Real-Time quantitative PCR revealed that the Clostridium leptum and C. coccoides populations increased in equol producers, while those of bifidobacteria and enterobacteria decreased, and vice versa in the non-producers. Finally, the Atopobium population increased in both groups, but especially in the non-producers at three months. As the main findings of this study, (i) variations in the microbial communities over the 6-month period of isoflavone supplementation were large; (ii) no changes in the fecal microbial populations that were convincingly treatment-specific were seen; and (iii) the production of equol did not appear to be associated with the presence of, or increase in the population of, any of the majority bacterial types analyzed.
This paper references
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(2007)
Metabolism of dietary soy isoflavones
J.-P (2007)
10.1111/j.1472-765X.2008.02408.x
Development of a real‐time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs
X. Guo (2008)
Profiling of complex
CHEM34953.0.CO (1993)
10.1093/JN/137.10.2242
Microbial and dietary factors are associated with the equol producer phenotype in healthy postmenopausal women.
S. Bolca (2007)
10.1093/nar/gkt1178
The carbohydrate-active enzymes database (CAZy) in 2013
V. Lombard (2014)
10.1093/nar/gkn663
The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics
B. Cantarel (2009)
Freezing fecal samples prior
M. I. Bahl (2012)
10.1093/JN/135.12.2786
Isoflavones and functional foods alter the dominant intestinal microbiota in postmenopausal women.
Thomas Clavel (2005)
10.1126/science.1224820
Intestinal Inflammation Targets Cancer-Inducing Activity of the Microbiota
Janelle C. Arthur (2012)
10.1128/AEM.72.2.1006-1012.2006
In Vitro Kinetic Analysis of Oligofructose Consumption by Bacteroides and Bifidobacterium spp. Indicates Different Degradation Mechanisms
R. Van der Meulen (2006)
Development of a real-time PCR
S. E. 004 Dennan (2006)
Intestinal inflammation targets cancer
J M. (2012)
Role of phytoestrogens
T. R. Nagy (2010)
10.1093/JN/136.8.2188
Method of defining equol-producer status and its frequency among vegetarians.
K. Setchell (2006)
10.1128/AEM.70.12.7220-7228.2004
Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces
T. Matsuki (2004)
10.1111/J.1574-6941.2006.00190.X
Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen.
S. Denman (2006)
postmenopausal women
T. Clavel (2007)
Phenolics in human nutrition: importance
J. O. Mapesa (2013)
10.1128/AEM.59.3.695-700.1993
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
G. Muyzer (1993)
10.1038/nprot.2006.142
Isolation of DNA from bacterial samples of the human gastrointestinal tract
E. Zoetendal (2006)
10.1055/s-0030-1250074
Role of phytoestrogens in cancer therapy.
Mandeep K. Virk-Baker (2010)
Cord blood cytokines and acute
B Ruiz-Pérez (2007)
10.1002/MNFR.200600262
Metabolism of dietary soy isoflavones to equol by human intestinal microflora--implications for health.
J. Yuan (2007)
Soyfoods and shoybean phyto - oestrogens ( isoflavones ) as possible alternatives to hormone replacement therapy
M. Messina (2000)
10.1007/s11101-013-9329-x
Soy isoflavones and their relationship with microflora: beneficial effects on human health in equol producers
J. Sánchez-Calvo (2013)
10.1021/jf100901b
Non-isoflavone phytochemicals in soy and their health effects.
J. Kang (2010)
10.1128/AEM.68.2.673-690.2002
Culture-Independent Analysis of Gut Bacteria: the Pig Gastrointestinal Tract Microbiota Revisited
T. Leser (2002)
10.2217/WHE.09.80
Influence of Sex Hormones and Phytoestrogens on Heart Disease in Men and Women
Poornima Bhupathy (2010)
Gut bacterial metabolism of the soy isoflavone daidzein : exploring the relevance to human nutrition
C. Atkinson (2005)
Influence of sex hormones
C. D. Haines (2010)
10.1542/peds.2006-0524
Cord Blood Cytokines and Acute Lower Respiratory Illnesses in the First Year of Life
N. Ly (2007)
10.1099/mic.0.042127-0
Novel approaches for analysing gut microbes and dietary polyphenols: challenges and opportunities.
R. Kemperman (2010)
10.1128/AEM.68.1.114-123.2002
Molecular Diversity of Lactobacillus spp. and Other Lactic Acid Bacteria in the Human Intestine as Determined by Specific Amplification of 16S Ribosomal DNA
H. Heilig (2002)
10.1128/AEM.05895-11
In Vitro Fermentation of Sugar Beet Arabino-Oligosaccharides by Fecal Microbiota Obtained from Patients with Ulcerative Colitis To Selectively Stimulate the Growth of Bifidobacterium spp. and Lactobacillus spp.†
Louise Kristine Vigsn�s (2011)
10.1111/j.1574-6968.2012.02523.x
Freezing fecal samples prior to DNA extraction affects the Firmicutes to Bacteroidetes ratio determined by downstream quantitative PCR analysis.
M. Bahl (2012)
10.1177/153537020523000302
Gut Bacterial Metabolism of the Soy Isoflavone Daidzein: Exploring the Relevance to Human Health
C. Atkinson (2005)
Isoflavones, equol, and fecal microbial populations
10.1016/j.jnutbio.2011.03.002
Equol production changes over time in postmenopausal women.
A. Franke (2012)
10.1038/nbt.2942
An integrated catalog of reference genes in the human gut microbiome
J. Li (2014)
10.1207/S15327914NC3601_5
Interindividual Variation in Metabolism of Soy Isoflavones and Lignans: Influence of Habitual Diet on Equol Production by the Gut Microflora
I. Rowland (2000)
10.1016/j.micres.2009.09.002
Antibacterial, antifungal, and antiviral activities of some flavonoids.
D. D. Orhan (2010)
10.1016/J.MICRES.2006.09.004
Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.
J. Delroisse (2008)
Determination of serum creatinine
G. Tiderström (1973)
10.1093/JN/132.12.3577
The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones.
K. Setchell (2002)
10.1016/J.SYAPM.2006.02.003
Clostridium saccharogumia sp. nov. and Lactonifactor longoviformis gen. nov., sp. nov., two novel human faecal bacteria involved in the conversion of the dietary phytoestrogen secoisolariciresinol diglucoside.
T. Clavel (2007)
10.1016/0009-8981(73)90466-X
Determination of serum creatinine by a direct colorimetric method.
D. Heinegård (1973)
10.1039/b802662a
Dietary phenolics: chemistry, bioavailability and effects on health.
A. Crozier (2009)
Dietary phenolics: chemistry
A. Crozier (2009)
10.1111/J.1574-6941.2007.00330.X
Metabolism of isoflavones, lignans and prenylflavonoids by intestinal bacteria: producer phenotyping and relation with intestinal community.
S. Possemiers (2007)
10.3945/an.111.000539
O-desmethylangolensin: the importance of equol's lesser known cousin to human health.
C. Frankenfeld (2011)
10.1016/J.FOODCHEM.2007.09.072
In vitro and in vivo studies on the antioxidant activities of the aqueous extracts of Douchi (a traditional Chinese salt-fermented soybean food)
D. Wang (2008)
10.1016/j.maturitas.2009.12.019
Controversies concerning the use of phytoestrogens in menopause management: bioavailability and metabolism.
P. de Cremoux (2010)
10.1038/nature08821
A human gut microbial gene catalogue established by metagenomic sequencing
J. Qin (2010)
10.1371/journal.pone.0108924
Fecal Bacterial Community Changes Associated with Isoflavone Metabolites in Postmenopausal Women after Soy Bar Consumption
C. Nakatsu (2014)
Molecular diversity of Lactobacillus spp
D A. (2002)
10.1016/S0140-6736(88)92338-0
GUT BACTERIAL METABOLISM
D. Flannery (1988)
Novel approaches for analyzing gut microbes and dietary polyphenols : challenges and opportunities
R. A. Kemperman (2010)
DES exposure: a continuing disaster.
Wallgren Dr (1981)
10.1128/AEM.68.11.5445-5451.2002
Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces
T. Matsuki (2002)
10.1128/AEM.67.2.504-513.2001
Bifidobacterial Diversity in Human Feces Detected by Genus-Specific PCR and Denaturing Gradient Gel Electrophoresis
R. Satokari (2001)
10.1128/AEM.01224-06
Sensitive Quantitative Detection of Commensal Bacteria by rRNA-Targeted Reverse Transcription-PCR
K. Matsuda (2006)
10.1078/072320203770865864
Identification of Bifidobacterium species using rep-PCR fingerprinting.
L. Masco (2003)
10.1007/978-3-642-22144-6_94
Phenolics in Human Nutrition: Importance of the Intestinal Microbiome for Isoflavone and Lignan Bioavailability 78
T. Clavel (2013)
10.1016/S0959-8049(00)00233-1
Soyfoods and soybean phyto-oestrogens (isoflavones) as possible alternatives to hormone replacement therapy (HRT).
M. Messina (2000)



This paper is referenced by
10.1016/j.maturitas.2017.01.013
Broadening our perspectives on complementary and alternative medicine for menopause: A narrative review.
Dunia Tonob (2017)
10.3389/fmicb.2017.01155
Development and Use of a Real-Time Quantitative PCR Method for Detecting and Quantifying Equol-Producing Bacteria in Human Faecal Samples and Slurry Cultures
L. Vázquez (2017)
10.1111/ggi.13029
Cross‐sectional study of equol producer status and cognitive impairment in older adults
M. Igase (2017)
10.3389/fmicb.2018.03002
Non-isoflavones Diet Incurred Metabolic Modifications Induced by Constipation in Rats via Targeting Gut Microbiota
J. Liu (2018)
10.1111/jpc.13934
Soy protein supplement intake for 12 months has no effect on sexual maturation and may improve nutritional status in pre‐pubertal children
Sandra M Duitama (2018)
10.3390/nu9101146
Effect of Curcumin on the Diversity of Gut Microbiota in Ovariectomized Rats
Z. Zhang (2017)
10.1093/ndt/gfaa076
Contribution of uremic dysbiosis to insulin resistance and sarcopenia.
K. Uchiyama (2020)
10.1016/j.foodres.2018.06.016
Host: Microbiome co-metabolic processing of dietary polyphenols - An acute, single blinded, cross-over study with different doses of apple polyphenols in healthy subjects.
K. Trôst (2018)
10.1111/1750-3841.14661
Effects of Lactic Acid Bacteria-Fermented Soymilk on Isoflavone Metabolites and Short-Chain Fatty Acids Excretion and Their Modulating Effects on Gut Microbiota.
Shuhong Dai (2019)
10.3389/fimmu.2019.01819
Fecal Changes Following Introduction of Milk in Infants With Outgrowing Non-IgE Cow's Milk Protein Allergy Are Influenced by Previous Consumption of the Probiotic LGG
Lucía Guadamuro (2019)
10.1016/j.jff.2020.103819
Modulation of equol production via different dietary regimens in an artificial model of the human colon
L. Vázquez (2020)
10.3390/nu10101481
Microbiota and Derived Parameters in Fecal Samples of Infants with Non-IgE Cow’s Milk Protein Allergy under a Restricted Diet
María Díaz (2018)
10.3390/ijms22010218
The Role of Isoflavones in Type 2 Diabetes Prevention and Treatment—A Narrative Review
Alina Kuryłowicz (2020)
10.1186/s12866-017-1001-y
Bacterial communities and metabolic activity of faecal cultures from equol producer and non-producer menopausal women under treatment with soy isoflavones
Lucía Guadamuro (2017)
10.1016/B978-0-12-811413-1.00002-4
The Importance of Microbial and Enzymatic Bioconversions of Isoflavones in Bioactive Compounds
D. B. Lopes (2017)
10.31665/JFB.2018.3153
Gut microbiota, probiotics, prebiotics and bone health: a review
N. Shang (2018)
10.3945/an.116.013623
Interindividual Variability in Biomarkers of Cardiometabolic Health after Consumption of Major Plant-Food Bioactive Compounds and the Determinants Involved
D. Milenkovic (2017)
10.4018/978-1-5225-0591-4.CH011
Soy and soy products, isoflavones, equol, and health
B. Pérez (2017)
10.3390/nu11081780
Gender Differences in Phytoestrogens and the Relationship with Speed of Processing in Older Adults: A Cross-Sectional Analysis of NHANES, 1999–2002
J. Alwerdt (2019)
10.1080/09637486.2017.1353955
Incomplete metabolism of phytoestrogens by gut microbiota from children under the age of three
P. Gaya (2018)
10.1002/jsfa.9648
Fermentation product with new equol-producing Lactobacillus paracasei as a probiotic-like product candidate for prevention of skin and intestinal disorder.
J. E. Kwon (2019)
10.1016/j.biotechadv.2020.107576
Biotransformation of dietary phytoestrogens by gut microbes: A review on bidirectional interaction between phytoestrogen metabolism and gut microbiota.
Ahkam Saddam Seyed Hameed (2020)
10.22159/IJAP.2018V10I6.29156
INFLUENCE OF PROBIOTIC SUPPLEMENTATION ON CLIMACTERIC SYMPTOMS IN MENOPAUSAL WOMEN-A MINI REVIEW
B. Sivamaruthi (2018)
10.1016/j.jep.2016.09.027
Qualitatively and quantitatively investigating the regulation of intestinal microbiota on the metabolism of panax notoginseng saponins.
Jingcheng Xiao (2016)
10.1021/ACS.JAFC.6B03725
Soy and Gut Microbiota: Interaction and Implication for Human Health.
Haiqiu Huang (2016)
10.1186/s12263-017-0585-z
Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats
Seong-Yeop Jeong (2017)
10.3390/biom10060950
Metabolism of Soy Isoflavones by Intestinal Bacteria: Genome Analysis of an Adlercreutzia equolifaciens Strain That Does Not Produce Equol
L. Vázquez (2020)
10.1111/bjd.17931
Gut microbiota dysbiosis in a cohort of patients with psoriasis
C. Hidalgo-Cantabrana (2019)
10.1016/bs.afnr.2020.06.002
Polyphenols, the new frontiers of prebiotics.
F. Nazzaro (2020)
10.1016/J.JFF.2017.12.043
Impact of microbiota on the use and effects of isoflavones in the relief of climacteric symptoms in menopausal women – A review
Naice Eleidiane Santana Monteiro (2018)
10.3389/fmicb.2020.01221
Colonization Potential to Reconstitute a Microbe Community in Pseudo Germ-Free Mice After Fecal Microbe Transplant From Equol Producer
Wenou Liang (2020)
10.1021/acs.jafc.5b05102
Profiling of Phenolic Metabolites in Feces from Menopausal Women after Long-Term Isoflavone Supplementation.
Lucía Guadamuro (2016)
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