Online citations, reference lists, and bibliographies.
← Back to Search

1-Aminocyclopropane-1-Carboxylate Oxidase Induction In Tomato Flower Pedicel Phloem And Abscission Related Processes Are Differentially Sensitive To Ethylene

M. Chersicola, A. Kladnik, M. Tušek Žnidarič, T. Mrak, K. Gruden, M. Dermastia
Published 2017 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Ethylene has impact on several physiological plant processes, including abscission, during which plants shed both their vegetative and reproductive organs. Cell separation and programmed cell death are involved in abscission, and these have also been correlated with ethylene action. However, the detailed spatiotemporal pattern of the molecular events during abscission remains unknown. We examined the expression of two tomato ACO genes, LeACO1, and LeACO4 that encode the last enzyme in ethylene biosynthesis, 1-aminocyclopropane-1-carboxylate oxidase (ACO), together with the expression of other abscission-associated genes involved in cell separation and programmed cell death, during a period of 0–12 h after abscission induction in the tomato flower pedicel abscission zone and nearby tissues. In addition, we determined their localization in specific cell layers of the flower pedicel abscission zone and nearby tissues obtained by laser microdissection before and 8 h after abscission induction. The expression of both ACO genes was localized to the vascular tissues in the pedicel. While LeACO4 was more uniformly expressed in all examined cell layers, the main expression site of LeACO1 was in cell layers just outside the abscission zone in its proximal and distal part. We showed that after abscission induction, ACO1 protein was synthesized in phloem companion cells, in which it was localized mainly in the cytoplasm. Samples were additionally treated with 1-methylcyclopropene (1-MCP), a competitive inhibitor of ethylene actions, and analyzed 8 h after abscission induction. Cell-layer-specific changes in gene expression were observed together with the specific localization and ethylene sensitivity of the hallmarks of cell separation and programmed cell death. While treatment with 1-MCP prevented separation of cells through inhibition of the expression of polygalacturonases, which are the key enzymes involved in degradation of the middle lamella, this had less impact on the occurrence of different kinds of membrane vesicles and abscission-related programmed cell death. In the flower pedicel abscission zone, the physical progressions of cell separation and programmed cell death are perpendicular to each other and start in the vascular tissues.
This paper references
Transcriptome Analysis of Tomato Flower Pedicel Tissues Reveals Abscission Zone-Specific Modulation of Key Meristem Activity Genes
X. Wang (2013)
Molecular mechanisms controlling plant organ abscission
T. Nakano (2013)
Role of auxin depletion in abscission control
S. Meir (2015)
Further examination of abscission zone cells as ethylene target cells in higher plants.
Michael T McManus (2008)
Organ abscission , ” in
M. E. Leslie (2007)
Organ abscission
M E Leslie (2007)
Examination of the Abscission-Associated Transcriptomes for Soybean, Tomato, and Arabidopsis Highlights the Conserved Biosynthesis of an Extensible Extracellular Matrix and Boundary Layer
Joonyup Kim (2015)
Expression profiling of tomato pre-abscission pedicels provides insights into abscission zone properties Frontiers in Plant Science
T. Nakano (2013)
IDA-like gene expression in soybean and tomato leaf abscission and requirement for a diffusible stelar abscission signal
M. Tucker (2012)
Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein
S. Liljegren (2009)
A Role for the Stele in Intertissue Signaling in the Initiation of Abscission in Bean Leaves (Phaseolus vulgaris L.)
D. S. Thompson (1994)
Endoreduplication preferentially occurs at the proximal side of the abscission zone during abscission of tomato leaf
M. Dermastia (2012)
Development and regulation of pedicel abscission in tomato
Y. Ito (2015)
The tomato genome sequence provides insights into fleshy fruit evolution
S. Sato (2012)
Abscission, dehiscence, and other cell separation processes.
J. Roberts (2002)
Regulation of ethylene biosynthesis at the level of 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene
I. Ruduś (2012)
Targeted Systems Biology Profiling of Tomato Fruit Reveals Coordination of the Yang Cycle and a Distinct Regulation of Ethylene Biosynthesis during Postclimacteric Ripening1[C][W][OA]
B. Van de Poel (2012)
Signals in abscission
J. E. Taylor (2001)
Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion1[C][W][OA]
S. Meir (2010)
Subcellular localization of 1-aminocyclopropane-1-carboxylate oxidase in tomato cells
D. Reinhardt (2004)
Genome-Wide Identification and Validation of Reference Genes in Infected Tomato Leaves for Quantitative RT-PCR Analyses
O. Müller (2015)
Suppression of LX Ribonuclease in Tomato Results in a Delay of Leaf Senescence and Abscission1[W]
A. Lers (2006)
Abscission research: what we know and what we still need to study
M. Tucker (2015)
Reactive oxygen species regulate leaf pulvinus abscission zone cell separation in response to water-deficit stress in cassava
Wenbin Liao (2016)
Relative quantification,” in Real-time PCR, ed T
M. W. Pfaffl (2006)
Subcellular localization of 1-aminocyclopropane-1-carboxylic acid oxidase in apple fruit.
M. Chung (2002)
Tomato (Solanum lycopersicum): A Model Fruit-Bearing Crop.
S. Kimura (2008)
Real-Time PCR
S. Bustin (2005)
Programmed Cell Death Occurs Asymmetrically during Abscission in Tomato[C][W][OA]
Tal Bar-Dror (2011)
A Conserved Core of Programmed Cell Death Indicator Genes Discriminates Developmentally and Environmentally Induced Programmed Cell Death in Plants1[OPEN]
Yadira Olvera-Carrillo (2015)
AoB Plants 2012:pls035
B. Van de Poel (2012)
Relative quantification
M. Pfaffl (2006)
6. Organ Abscission
Michelle E. Leslie (2007)
Organ abscission,” in Plant Cell Separation and Adhesion, Annual Plant Reviews, Vol
M. E. Leslie (2007)
Regulation of ripening and opportunities for control in tomato and other fruits.
G. Seymour (2013)
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
Three Different Polygalacturonases Are Expressed in Tomato Leaf and Flower Abscission, Each with a Different Temporal Expression Pattern
P. Kalaitzis (1997)
Ethylene and programmed cell death in plants
Christopher P. Trobacher (2009)
Transcriptome Analysis of Soybean Leaf Abscission Identifies Transcriptional Regulators of Organ Polarity and Cell Fate
Joonyup Kim (2016)
Temporal transcriptional response to ethylene gas drives growth hormone cross-regulation in Arabidopsis
K. N. Chang (2013)
ROS-mediated abiotic stress-induced programmed cell death in plants
V. Petrov (2015)
Genetics and Control of Tomato Fruit_Ripening and Quality Attributes
G. Gianquinto (2015)
Treatment of Fruit with Propylene gives Information about the Biogenesis of Ethylene
E. Mcmurchie (1972)
This is an Open Access article distribut...
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
Expression profiling of tomato pre-abscission pedicels provides insights into abscission zone properties including competence to respond to abscission signals
T. Nakano (2012)
1-aminocyclopropane-1-carboxylate oxidase of apple fruit is periplasmic
S. Ramassamy (1998)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar