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Graphene Oxide Functional Nanohybrids With Magnetic Nanoparticles For Improved Vectorization Of Doxorubicin To Neuroblastoma Cells

Luigi Lerra, Annafranca Farfalla, Beatriz Sanz, Giuseppe Cirillo, Orazio Vittorio, Florida Voli, Marion Le Grand, Manuela Curcio, Fiore Nicoletta, Anna Dubrovska, Silke Hampel, Francesca Iemma, Gerardo Goya

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With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin–human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the MNPs@GO system and albumin bioconjugate, consisted of MNPs@GO nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44–0.88 µM), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site.