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Biological Properties Of Iron Oxide Nanoparticles For Cellular And Molecular Magnetic Resonance Imaging

T. Schlorf, Manuela Meincke, E. Kossel, C. Glüer, O. Jansen, R. Mentlein
Published 2010 · Chemistry, Medicine

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Superparamagnetic iron-oxide particles (SPIO) are used in different ways as contrast agents for magnetic resonance imaging (MRI): Particles with high nonspecific uptake are required for unspecific labeling of phagocytic cells whereas those that target specific molecules need to have very low unspecific cellular uptake. We compared iron-oxide particles with different core materials (magnetite, maghemite), different coatings (none, dextran, carboxydextran, polystyrene) and different hydrodynamic diameters (20–850 nm) for internalization kinetics, release of internalized particles, toxicity, localization of particles and ability to generate contrast in MRI. Particle uptake was investigated with U118 glioma cells und human umbilical vein endothelial cells (HUVEC), which exhibit different phagocytic properties. In both cell types, the contrast agents Resovist, B102, non-coated Fe3O4 particles and microspheres were better internalized than dextran-coated Nanomag particles. SPIO uptake into the cells increased with particle/iron concentrations. Maximum intracellular accumulation of iron particles was observed between 24 h to 36 h of exposure. Most particles were retained in the cells for at least two weeks, were deeply internalized, and only few remained adsorbed at the cell surface. Internalized particles clustered in the cytosol of the cells. Furthermore, all particles showed a low toxicity. By MRI, monolayers consisting of 5000 Resovist-labeled cells could easily be visualized. Thus, for unspecific cell labeling, Resovist and microspheres show the highest potential, whereas Nanomag particles are promising contrast agents for target-specific labeling.
This paper references
10.2741/2796
An overview of responsive MRI contrast agents for molecular imaging.
Byunghee Yoo (2008)
10.1055/S-2005-858330
Untersuchungen zur Markierung von mesenchymalen Stammzellen mit unterschiedlichen superparamagnetischen Eisenoxidpartikeln und Nachweisbarkeit in der MRT bei 3T
H. Ittrich (2005)
Artefact-free imaging of iron oxide labeled monolayers with magnetic resonance at 3T
C. Heneweer (2006)
10.1007/s00216-010-4207-5
Nanoparticles as contrast agents for in-vivo bioimaging: current status and future perspectives
Megan A. Hahn (2011)
10.2741/2987
Iron oxide-loaded liposomes for MR imaging.
Manuela Meincke (2008)
10.1002/JMRI.1880070629
Uptake of dextran‐coated monocrystalline iron oxides in tumor cells and macrophages
A. Moore (1997)
Can receptors be imaged with MRI agents?
A. Nunn (1997)
10.1016/J.BIOMATERIALS.2005.04.041
Influence of polysaccharide coating on the interactions of nanoparticles with biological systems.
C. Lemarchand (2006)
10.1046/j.1471-4159.2002.01179.x
Pleiotrophin, an angiogenic and mitogenic growth factor, is expressed in human gliomas
R. Mentlein (2002)
10.1227/01.NEU.0000175731.25414.4c
Imaging, Distribution, and Toxicity of Superparamagnetic Iron Oxide Magnetic Resonance Nanoparticles in the Rat Brain and Intracerebral Tumor
L. Muldoon (2005)
10.1111/j.1471-4159.2005.03123.x
Enhanced expression and shedding of the transmembrane chemokine CXCL16 by reactive astrocytes and glioma cells
Andreas W. W. Ludwig (2005)
10.1007/s10334-005-0108-6
In vitro imaging of single living human umbilical vein endothelial cells with a clinical 3.0-T MRI scanner
Z. Zhang (2005)
[Labeling of mesenchymal stem cells with different superparamagnetic particles of iron oxide and detectability with MRI at 3T].
H. Ittrich (2005)
Imaging, distribution and toxicity of superparamagnetic iron oxide magnetic resonance agents in rat brain and intracerebral tumor
L. Muldoon (2005)
[Cell tracking. Principles and applications].
J. Grimm (2007)
10.1007/BF00239451
Binding and internalization of gold-conjugated somatostatin and growth hormone-releasing hormone in cultured rat somatotropes
R. Mentlein (2004)
10.1007/s00117-006-1449-5
Cell Tracking
J. Grimm (2006)
10.1016/j.biomaterials.2008.05.015
Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells.
D. Thorek (2008)
10.1002/jmri.20925
In vivo magnetic resonance imaging of iron oxide–labeled, arterially‐injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: Detection and monitoring at 3T
H. Ittrich (2007)



This paper is referenced by
10.1007/s11051-012-1091-2
Toxicity of iron oxide nanoparticles against osteoblasts
Sifeng Shi (2012)
10.1021/acsami.6b05840
Accelerated Blood Clearance Phenomenon Reduces the Passive Targeting of PEGylated Nanoparticles in Peripheral Arterial Disease.
Hyung-Jun Im (2016)
10.1038/srep36650
Erlotinib-Conjugated Iron Oxide Nanoparticles as a Smart Cancer-Targeted Theranostic Probe for MRI
A. A. I. Ali (2016)
10.1186/s12951-016-0190-0
Iron oxide nanoparticles for neuronal cell applications: uptake study and magnetic manipulations
Michal Marcus (2016)
10.1038/s41598-018-19628-z
Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings
Qiyi Feng (2018)
10.1021/la304872d
One-pot, exchange-free, room-temperature synthesis of sub-10 nm aqueous, noninteracting, and stable zwitterated iron oxide nanoparticles.
Zaki G. Estephan (2013)
Aspectos físicos e biológicos de nanopartículas de ferritas magnéticas
V. C. P. Fontanive (2015)
10.3109/17435390.2011.641604
Cytotoxicity, oxidative stress and expression of adhesion molecules in human umbilical vein endothelial cells exposed to dust from paints with or without nanoparticles
L. Mikkelsen (2013)
10.5772/1072
When Things Go Wrong - Diseases and Disorders of the Human Brain
Theo Mantamadiotis (2012)
10.1007/s11095-012-0711-y
Magnetic Nanoparticles in Magnetic Resonance Imaging and Diagnostics
Christine Rümenapp (2012)
10.1007/s11307-017-1093-7
Characterization of Magneto-Endosymbionts as MRI Cell Labeling and Tracking Agents
Kimberly D. Brewer (2017)
Development of reflectance imaging methodologies to investigate super-paramagnetic iron oxide nanoparticles
Emily J Guggenheim (2017)
10.1002/mabi.201200243
Biocompatible multishell architecture for iron oxide nanoparticles.
J. Wotschadlo (2013)
10.2174/1871520618666181109112655
Hybrid Magnetic Nanostructures For Cancer Diagnosis And Therapy.
I. L. Ardelean (2019)
10.1016/j.colsurfb.2017.10.038
Dynamic morphogenesis of dendritic structures formation in hen egg white lysozyme fibrils doped with magnetic nanoparticles.
N. Tomašovičová (2018)
10.1002/bio.3053
Application of a fluorescent biosensor based-on magneto-γ-Fe2 O3 -methyldopa nanoparticles for adsorption of human serum albumin.
N. Shahabadi (2016)
Biological Synthesis and Structural Characterization of Selenium Nanoparticles and Assessment of Their Antimicrobial Properties
B. El-Deeb (2018)
10.7150/thno.5411
Superparamagnetic Iron Oxide Nanoparticles: Amplifying ROS Stress to Improve Anticancer Drug Efficacy
G. Huang (2013)
10.1155/2012/218940
Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles
B. Castillo (2012)
10.1021/mp3002705
Maghemite functionalization for antitumor drug vehiculization.
K. Rudzka (2012)
10.7150/thno.11544
Magnetic Nanoparticles in Cancer Theranostics
O. Gobbo (2015)
10.1016/j.jconrel.2013.03.016
SPIO-PICsome: development of a highly sensitive and stealth-capable MRI nano-agent for tumor detection using SPIO-loaded unilamellar polyion complex vesicles (PICsomes).
D. Kokuryo (2013)
10.2174/138920021131400109
Pharmacokinetics and applications of magnetic nanoparticles.
Runyi Mao (2013)
10.1016/j.colsurfb.2019.05.008
Therapeutic and diagnostic potential of nanomaterials for enhanced biomedical applications.
M. Abd Elkodous (2019)
10.1088/0957-4484/27/11/115101
In vitro study on apoptotic cell death by effective magnetic hyperthermia with chitosan-coated MnFe₂O₄.
Yunok Oh (2016)
10.3390/magnetochemistry6010003
Magnetic Nanoparticles for Nanomedicine
M. Hepel (2020)
10.2217/nnm.14.232
Nanodiagnostics, nanopharmacology and nanotoxicology of platelet-vessel wall interactions.
K. Tomaszewski (2015)
10.2147/IJN.S30320
Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers
Wahajuddin (2012)
IRON OXIDE NANOPARTICLES AND THEIR TOXICOLOGICAL EFFECTS: IN VIVO AND IN VITRO STUDIES
Szalay Brigitta (2012)
10.1155/2013/137275
Synthesis and magnetic properties of a superparamagnetic nanocomposite pectin-magnetite nanocomposite
Jude E Namanga (2013)
Detection of neural stem cells function in rats with traumatic brain injury by manganese-enhanced magnetic resonance imaging.
H. Tang (2011)
10.2217/nnm.16.25
In focus: Fe3O4 nanoparticles and human mesenteric artery interaction in vitro.
T. Vukova (2016)
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