Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

The Hydroxytyrosol Induces The Death For Apoptosis Of Human Melanoma Cells

F. Costantini, C. Di Sano, G. Barbieri
Published 2020 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Melanoma is the most aggressive form of skin cancer and one of the most treatment-refractory malignancies. In metastatic melanoma cell lines, we analysed the anti-proliferative and pro-apoptotic potentials of a phenolic component of olive oil, the hydroxytyrosol. In particular, through MTS assay, DeadEnd™ Colorimetric TUNEL assay, Annexin V binding and PI uptake, western blot experiment, intracellular reactive oxygen species (ROS) analysis, and the cell colony assay, we showed that the hydroxytyrosol treatment remarkably reduces the cell viability inducing the death for apoptosis of melanoma cells. Moreover, we showed that the hydroxytyrosol treatment of melanoma cells leads to a significant increase of p53 and γH2AX expression, a significant decrease of AKT expression and the inhibition of cell colony formation ability. Finally, we propose that the increased amount of intracellular reactive oxygen species (ROS) that may be related to the regulation of the pathways involved in the activation of apoptosis and in the inhibition of melanoma growth could be the strategy used by hydroxytyrosol to exert its functions in melanoma. Therefore, for its role in melanoma growth inhibition, the hydroxytyrosol treatment could deeply interfere with melanoma progression as a promising therapeutic option for the treatment of this highly invasive tumour.
This paper references
10.1038/nature08467
The DNA-damage response in human biology and disease
S. Jackson (2009)
10.1016/j.ijpharm.2015.09.057
Development and in vitro assessment of alginate bilayer films containing the olive compound hydroxytyrosol as an alternative for topical chemotherapy.
Shiow-Fern Ng (2015)
10.1038/ncb2344
More than just a focus: The chromatin response to DNA damage and its role in genome integrity maintenance
J. Lukas (2011)
10.3390/molecules24102001
Hydroxytyrosol, Tyrosol and Derivatives and Their Potential Effects on Human Health
Ana Karković Marković (2019)
10.1039/C6FO00958A
Anti-cancer properties of olive oil secoiridoid phenols: a systematic review of in vivo studies.
R. Fabiani (2016)
10.1039/c5fo00932d
In vitro chemo-preventive activities of hydroxytyrosol: the main phenolic compound present in extra-virgin olive oil.
P. Rosignoli (2016)
10.1016/j.bcp.2012.03.018
The diverse roles and clinical relevance of PARPs in DNA damage repair: current state of the art.
M. De Vos (2012)
10.3892/ijmm.2017.3078
Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts
P. Kouka (2017)
10.1007/s00394-011-0230-3
Anti-proliferative and pro-apoptotic activities of hydroxytyrosol on different tumour cells: the role of extracellular production of hydrogen peroxide
R. Fabiani (2011)
10.1016/j.semcancer.2017.07.002
Regulation of cell signaling pathways by dietary agents for cancer prevention and treatment.
M. Pandey (2017)
10.1038/s41580-018-0089-8
Regulation of apoptosis in health and disease: the balancing act of BCL-2 family proteins
Rajinder Singh (2019)
10.1038/371346A0
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE
Y. Lazebnik (1994)
10.1016/j.freeradbiomed.2017.01.004
Reactive oxygen species and cancer paradox: To promote or to suppress?
S. Galadari (2017)
10.1016/j.canlet.2014.01.028
Hydroxytyrosol, a natural molecule from olive oil, suppresses the growth of human hepatocellular carcinoma cells via inactivating AKT and nuclear factor-kappa B pathways.
Baolei Zhao (2014)
Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note.
S. Kaufmann (1989)
10.1038/sj.onc.1208615
The p53 pathway: positive and negative feedback loops
S. Harris (2005)
10.1016/S1535-6108(03)00248-4
Targeting the PI3K-Akt pathway in human cancer: rationale and promise.
J. Luo (2003)
10.1021/JF00071A014
Importance and evolution of phenolic compounds in olive during growth and maturation
M. Amiot (1986)
10.1038/s41598-018-32308-2
Oxidative DNA damage induced by ROS-modulating agents with the ability to target DNA: A comparison of the biological characteristics of citrus pectin and apple pectin
F. Salehi (2018)
Dacarbazine-based chemotherapy for metastatic melanoma: thirty-year experience overview.
L. Serrone (2000)
10.1007/s10616-009-9191-2
Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells
J. Han (2009)
10.1021/JF000537W
Antioxidant activity of hydroxytyrosol acetate compared with that of other olive oil polyphenols.
M. Gordon (2001)
10.1016/j.ejca.2015.11.016
Immune-related adverse events with immune checkpoint blockade: a comprehensive review.
J. Michot (2016)
10.1001/ARCHNEUR.63.12.NOC60109
Mediterranean diet, Alzheimer disease, and vascular mediation.
N. Scarmeas (2006)
10.1074/jbc.273.10.5858
DNA Double-stranded Breaks Induce Histone H2AX Phosphorylation on Serine 139*
E. Rogakou (1998)
10.3390/ijms19071937
The Olive Biophenols Oleuropein and Hydroxytyrosol Selectively Reduce Proliferation, Influence the Cell Cycle, and Induce Apoptosis in Pancreatic Cancer Cells
C. Goldsmith (2018)
10.1186/1471-2482-13-S2-S14
Mediterranean diet and cancer: epidemiological evidence and mechanism of selected aspects
G. Grosso (2013)
10.1200/JCO.2000.18.1.158
Randomized phase III study of temozolomide versus dacarbazine in the treatment of patients with advanced metastatic malignant melanoma.
M. Middleton (2000)
10.1021/jf3026666
Analysis of total contents of hydroxytyrosol and tyrosol in olive oils.
C. Romero (2012)
Reactive oxygen species and cancer paradox: To promote or to suppress? Free Radic
S Galadari (2017)
10.3322/canjclin.39.6.399
Cancer statistics
N. Dubrawsky (1989)
10.1016/j.cell.2017.04.001
AKT/PKB Signaling: Navigating the Network
B. Manning (2017)
10.1038/nature22794
Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance
S. Shaffer (2017)
10.1002/jcp.27584
Hydroxytyrosol from olive fruits prevents blue‐light‐induced damage in human keratinocytes and fibroblasts
R. Avola (2019)
10.1038/nrc.2016.37
From melanocytes to melanomas
A. Shain (2016)
10.1111/1750-3841.14198
Antitumor Perspectives of Oleuropein and Its Metabolite Hydroxytyrosol: Recent Updates.
M. Imran (2018)
10.1007/s40618-016-0537-2
High doses of hydroxytyrosol induce apoptosis in papillary and follicular thyroid cancer cells
G. Toteda (2016)
10.1002/(SICI)1097-0320(19980101)31:1<1::AID-CYTO1>3.0.CO;2-R
Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure.
M. van Engeland (1998)
10.1002/ptr.2943
The protective effects of hydroxytyrosol against UVB‐induced DNA damage in HaCaT cells
W. Guo (2010)
10.3322/caac.21551
Cancer statistics, 2019
R. Siegel (2019)
10.3390/cells8121547
Dibutyltin(IV) and Tributyltin(IV) Derivatives of meso-Tetra(4-sulfonatophenyl)porphine Inhibit the Growth and the Migration of Human Melanoma Cells
F. Costantini (2019)
10.1016/j.foodchem.2020.126197
Antioxidant properties of two novel lipophilic derivatives of hydroxytyrosol.
T. M. Olajide (2020)
Mediterranean diet pyramid today
A. Bach-Faig (2011)
10.1002/PTR.1363
Plants used in cosmetics
T. Aburjai (2003)
10.1002/mnfr.201300323
Oleuropein and hydroxytyrosol activate GPER/ GPR30-dependent pathways leading to apoptosis of ER-negative SKBR3 breast cancer cells.
A. Chimento (2014)
10.1080/01635581.2016.1180407
Cytotoxic Activity of Oleocanthal Isolated from Virgin Olive Oil on Human Melanoma Cells
S. Fogli (2016)
10.1039/c4fo00187g
Hydroxytyrosol induces apoptosis in human colon cancer cells through ROS generation.
Lijuan Sun (2014)
Tyrosol and Derivatives and Their Potential Effects on Human Health
Karkovic Markovic (2001)
10.1016/S0070-2153(04)63001-8
Early events in the DNA damage response.
I. Ward (2004)
10.1038/nrm2256
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
B. D'autréaux (2007)
10.1111/bph.14778
Mediterranean diet and health status: Active ingredients and pharmacological mechanisms
L. Schwingshackl (2019)
10.1038/s41419-017-0059-7
Cell death-based treatments of melanoma:conventional treatments and new therapeutic strategies
G. Mattia (2017)
10.1017/S1368980011002515
Mediterranean diet pyramid today. Science and cultural updates.
A. Bach-Faig (2011)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar