Online citations, reference lists, and bibliographies.

Microfluidic Vortex Enhancement For On-Chip Sample Preparation

Anna Haller, Andreas Spittler, Lukas Brandhoff, Helene Zirath, Dietmar Puchberger-Enengl, Franz Keplinger, Michael J. Vellekoop
Published 2015 · Computer Science, Materials Science
Cite This
Download PDF
Analyze on Scholarcy
Share
In the past decade a large amount of analysis techniques have been scaled down to the microfluidic level. However, in many cases the necessary sample preparation, such as separation, mixing and concentration, remains to be performed off-chip. This represents a major hurdle for the introduction of miniaturized sample-in/answer-out systems, preventing the exploitation of microfluidic’s potential for small, rapid and accurate diagnostic products. New flow engineering methods are required to address this hitherto insufficiently studied aspect. One microfluidic tool that can be used to miniaturize and integrate sample preparation procedures are microvortices. They have been successfully applied as microcentrifuges, mixers, particle separators, to name but a few. In this work, we utilize a novel corner structure at a sudden channel expansion of a microfluidic chip to enhance the formation of a microvortex. For a maximum area of the microvortex, both chip geometry and corner structure were optimized with a computational fluid dynamic (CFD) model. Fluorescent particle trace measurements with the optimized design prove that the corner structure increases the size of the vortex. Furthermore, vortices are induced by the corner structure at low flow rates while no recirculation is observed without a corner structure. Finally, successful separation of plasma from human blood was accomplished, demonstrating a potential application for clinical sample preparation. The extracted plasma was characterized by a flow cytometer and compared to plasma obtained from a standard benchtop centrifuge and from chips without a corner structure.
This paper references
Microfluidic devices for blood fractionation. Micromachines 2011
H W Hou
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons
10.1007/s10404-007-0238-4
A high-efficiency planar micromixer with convection and diffusion mixing over a wide Reynolds number range
T. R. Shih (2008)
10.1002/smll.201302907
Microfluidic blood cell sorting: now and beyond.
Zeta Tak For Yu (2014)
10.1021/nl2032487
Selective trapping and manipulation of microscale objects using mobile microvortices.
Tristan Petit (2012)
10.1021/nl301253v
Mass production and size control of lipid-polymer hybrid nanoparticles through controlled microvortices.
Yong Tae Kim (2012)
10.1063/1.2409629
Microfluidic blood plasma separation via bulk electrohydrodynamic flows.
Dian R. Arifin (2007)
10.1007/s00216-006-0611-2
Cellular manipulations in microvortices
Daniel T Chiu (2006)
10.1039/c3lc50689d
Size-selective collection of circulating tumor cells using Vortex technology.
Elodie Sollier (2014)
10.1002/bit.22833
Continuous scalable blood filtration device using inertial microfluidics.
Albert J. Mach (2010)
10.1039/b813952k
Continuous focusing of microparticles using inertial lift force and vorticity via multi-orifice microfluidic channels.
Jungwon Park (2009)
10.1007/S10404-011-0864-8
Formation of recirculation zones in a sudden expansion microchannel with a rectangular block structure over a wide Reynolds number range
Chien Hsiung Tsai (2012)
10.1039/b926834k
Validation of a blood plasma separation system by biomarker detection.
Maïwenn Kersaudy-Kerhoas (2010)
10.1021/ac403688g
Micro total analysis systems: fundamental advances and biological applications.
Christopher T Culbertson (2014)
10.1007/S10404-013-1166-0
Mapping low-Reynolds-number microcavity flows using microfluidic screening devices
Rami Fishler (2013)
10.1039/c2lc41104k
Microfluidic sample preparation for diagnostic cytopathology.
Albert J. Mach (2013)
10.1038/425038a
Microfluidic systems: High radial acceleration in microvortices
J. Patrick Shelby (2003)
10.1039/b821247c
Self-powered microfluidic chips for multiplexed protein assays from whole blood.
Lidong Qin (2009)
10.1063/1.4818906
Vortex-aided inertial microfluidic device for continuous particle separation with high size-selectivity, efficiency, and purity.
Xiao Wang (2013)
10.1039/b813434k
Microvortex for focusing, guiding and sorting of particles.
Chia-Hsien Hsu (2008)
10.1115/1.1760532
Backward-Facing Step Flows for Various Expansion Ratios at Low and Moderate Reynolds Numbers
Gautam Biswas (2004)
Investigations of vortex formation in microbifurcations
Ca ˘ ta (2012)
10.1039/c1lc20330d
Automated cellular sample preparation using a Centrifuge-on-a-Chip.
Albert J. Mach (2011)
10.1039/c4lc00128a
Inertial microfluidic physics.
Hamed Amini (2014)
10.1039/b402479f
Controlled rotation of biological micro- and nano-particles in microvortices.
J. Patrick Shelby (2004)
10.1007/S10404-013-1296-4
Multi-step microfluidic system for blood plasma separation: architecture and separation efficiency
Julien Marchalot (2014)
10.3390/mi2030319
Microfluidic Devices for Blood Fractionation
Han Wei Hou (2011)
10.1039/c3lc50432h
Micro-scale blood plasma separation: from acoustophoresis to egg-beaters.
Maïwenn Kersaudy-Kerhoas (2013)
10.1007/s10404-013-1176-y
Enhanced size-dependent trapping of particles using microvortices
Jian Zhou (2013)
10.1109/SENSOR.2007.4300283
Microfludic Centrifuge of Nano Particles using Rotating Flow in a Microchamber
Jin Bong Ha (2007)
10.3390/mi3020529
Simulation and Experimental Characterization of Microscopically Accessible Hydrodynamic Microvortices
Wenjie Zhang (2012)
10.1007/S10404-011-0875-5
Microfluidic centrifuge based on a counterflow configuration
Natalya Pertaya-Braun (2012)
Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma.
Magalie Faivre (2006)
10.1002/1522-2683(200203)23:5<677::AID-ELPS677>3.0.CO;2-8
Microfluidic chips for clinical and forensic analysis.
Elisabeth M.J. Verpoorte (2002)
10.1063/1.3689939
A practical guide for the fabrication of microfluidic devices using glass and silicon.
Ciprian Iliescu (2012)
10.1007/S00348-014-1758-9
Vortex generation in a microfluidic chamber with actuations
Xiaopeng Shang (2014)
10.1016/j.ces.2008.11.011
Mixing efficiency of a multilamination micromixer with consecutive recirculation zones
Jongkwang Lee (2009)
10.1063/1.4771407
Bacterial aggregation and biofilm formation in a vortical flow.
Shahrzad Yazdi (2012)
10.1063/1.4704504
High-performance microfluidic rectifier based on sudden expansion channel with embedded block structure.
Chien-Hsiung Tsai (2012)
10.1007/S10404-013-1291-9
Particle separation and sorting in microfluidic devices: a review
Pattammadath Sajeesh (2014)
10.1007/S10404-013-1200-2
Study on microchannel flows with a sudden contraction–expansion at a wide range of Knudsen number using lattice Boltzmann method
T. S. Liou (2014)
10.1007/s10544-010-9456-8
Rapid multivortex mixing in an alternately formed contraction-expansion array microchannel
Myung Gwon Lee (2010)
10.1007/S10404-014-1343-9
Unsteady pulsating characteristics of the fluid flow through a sudden expansion microvalve
Amir Nejat (2014)
10.1103/PHYSREVE.82.026308
Dynamic particle trapping, release, and sorting by microvortices on a substrate.
S. Liu (2010)
10.1007/s10544-010-9405-6
Fast and continuous plasma extraction from whole human blood based on expanding cell-free layer devices
Elodie Sollier (2010)
10.1016/J.PROENG.2011.12.109
Towards an optimized blood plasma separation chip: Finite element analysis of a novel corner structure in a backward-facing step
Anna Haller (2011)
10.1016/j.snb.2009.05.023
Passive microfluidic devices for plasma extraction from whole human blood
Elodie Sollier (2008)
10.1063/1.2212275
Electrohydrodynamic surface microvortices for mixing and particle trapping
Leslie Y. Yeo (2006)
10.1063/1.3576780
High-throughput size-based rare cell enrichment using microscale vortices.
Soojung Claire Hur (2011)



This paper is referenced by
10.1039/c6lc00833j
Microfluidic blood plasma separation for medical diagnostics: is it worth it?
Witold S. Mielczarek (2016)
10.1177/2211068216663604
Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry
Akhil Chaturvedi (2017)
10.3390/mi9030123
Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment
Akshay Kale (2018)
10.1021/acs.analchem.6b03624
Bedside Immune Monitoring: An Automated Immunoassay Platform for Quantification of Blood Biomarkers in Patient Serum within 20 Minutes.
Helene Zirath (2017)
10.1016/J.CES.2017.05.040
Numerical and experimental investigations of chaotic mixing behavior in an oscillating feedback micromixer
Tingliang Xie (2017)
10.1016/J.MOLLIQ.2017.12.094
Effect of wall material on water nanovortices formation in 2D long open type nanocavity. Molecular Dynamics study
A. Kordos (2018)
10.1063/1.5031082
Virtual vortex gear: Unique flow patterns driven by microfluidic inertia leading to pinpoint injection.
Chia-Hung Dylan Tsai (2018)
10.1016/J.MOLLIQ.2017.11.003
Water nanovortices formation in 2D open type long nanocavities. Molecular dynamics study
Anna Kucaba-Piętal (2018)
10.24425/119065
Nanovortex evolution in entrance part of the 2D open type long nanocavity
A. Kordos (2018)
10.1002/smll.201903605
On-Chip Generation of Vortical Flows for Microfluidic Centrifugation.
Heba Omar Ahmed (2019)
10.1021/acsami.7b04252
Information Visualization and Feature Selection Methods Applied to Detect Gliadin in Gluten-Containing Foodstuff with a Microfluidic Electronic Tongue.
Cristiane M. Daikuzono (2017)
10.1002/elps.201800043
Three-dimensional microfluidic chip with twin-layer herringbone structure for high efficient tumor cell capture and release via antibody-conjugated magnetic microbeads.
Lei Zhang (2018)
10.3390/mi7030044
Micromachines Beyond Silicon-Based Technologies: A Letter from the New Editor-in-Chief
Nam-Trung Nguyen (2016)
Sensor Systems for Impaired Healing Markers, Concepts and Applications for Objective Wound Assessment
Dietmar Puchberger (2016)
10.1007/S10404-018-2056-2
Effects of geometry factors on microvortices evolution in confined square microcavities
Feng Long Shen (2018)
10.1063/1.5017753
Analysis of micro-fluidic tweezers in the Stokes regime
Longhua Zhao (2016)
Semantic Scholar Logo Some data provided by SemanticScholar