Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Development Of A Sphingosylphosphorylcholine Detection System Using RNA Aptamers

Katsunori Horii, K. Omi, Yoshihito Yoshida, Yuka Imai, N. Sakai, A. Oka, H. Masuda, M. Furuichi, Tetsuji Tanimoto, I. Waga
Published 2010 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC.
This paper references
Sphingosylphosphorylcholine is a remarkably potent mitogen for a variety of cell lines.
N. Desai (1991)
Binding and disruption of phospholipid bilayers by supramolecular RNA complexes
A. Vlassov (2001)
Elevated concentrations of sphingosylphosphorylcholine in cerebrospinal fluid after subarachnoid hemorrhage: A possible role as a spasmogen
T. Kurokawa (2009)
The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphingomyelin deacylase, which cleaves the N-acyl linkage of sphingomyelin and glucosylceramide.
K. Higuchi (2000)
Sphingosylphosphorylcholinebiological functions and mechanisms of action
D. Meyer zu Heringdorf (2002)
Sphingosylphosphorylcholine-biological functions and mechanisms of action.
D. Meyer Zu Heringdorf (2002)
RNAs that bind and change the permeability of phospholipid membranes.
A. Khvorova (1999)
Delayed cerebral vasospasm and nitric oxide: review, new hypothesis, and proposed treatment.
R. Pluta (2005)
Sample Availability: Samples of the compounds are available from the authors
In vitro selection of RNA molecules that bind specific ligands
A. Ellington (1990)
Sphingosylphosphorylcholine is a novel messenger for Rho-kinase-mediated Ca[2+] sensitization in the bovine cerebral artery : Unimportant role for protein kinase C
白尾 敏之 (2003)
Sphingosylphosphorylcholine is upregulated in the stratum corneum of patients with atopic dermatitis Published, JLR Papers in Press, October 1, 2002. DOI 10.1194/jlr.M200225-JLR200
R. Okamoto (2003)
Sphingosylphosphorylcholine stimulates proliferation and upregulates cell surface-associated plasminogen activator activity in cultured human keratinocytes.
H. Wakita (1998)
Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance.
T. Misono (2005)
Sphingolipidomics: high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry.
A. Merrill (2005)
Sphingosylphosphocholine is a naturally occurring lipid mediator in blood plasma: a possible role in regulating cardiac function via sphingolipid receptors.
K. Liliom (2001)
In vitro selection of RNA lectins: using combinatorial chemistry to interpret ribozyme evolution.
S. Lato (1995)
PseudoViewer3: generating planar drawings of large-scale RNA structures with pseudoknots
Yanga Byun (2009)
Amino Acids as RNA Ligands: A Direct-RNA-Template Theory for the Code's Origin
M. Yarus (1998)
Sphingolipids differentially regulate mitogen‐activated protein kinases and intracellular Ca2+ in vascular smooth muscle: effects on CREB activation
F. A. Mathieson (2006)
Sphingosylphosphorylcholine Is a Novel Messenger for Rho-Kinase–Mediated Ca2+ Sensitization in the Bovine Cerebral Artery: Unimportant Role for Protein Kinase C
S. Shirao (2002)
Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure.
D. Mathews (2004)
Sphingosine 1-phosphate, a bioactive sphingolipid abundantly stored in platelets, is a normal constituent of human plasma and serum.
Y. Yatomi (1997)
Functions of the Multifaceted Family of Sphingosine Kinases and Some Close Relatives*
S. Spiegel (2007)
Aptamers That Recognize the Lipid Moiety of the Antibiotic Moenomycin A
Heike Betat (2003)
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
C. Tuerk (1990)
Sphingosylphosphorylcholine is a potent inducer of intercellular adhesion molecule-1 expression in human keratinocytes.
G. Imokawa (1999)
A rapid and quantitative LC-MS/MS method to profile sphingolipids
M. Scherer (2010)
Sphingosylphosphorylcholine induces endothelial cell migration and morphogenesis.
G. Boguslawski (2000)
The multi-functional role of sphingosylphosphorylcholine.
G. Nixon (2008)
This article is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution license

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar