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Facile And Controllable Fabrication Of Protein-Only Nanoparticles Through Photo-Induced Crosslinking Of Albumin And Their Application As DOX Carriers

X. Long, J. Ren, C. Zhang, F. Ji, Lingyun Jia
Published 2019 · Chemistry, Medicine

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Protein-based nanoparticles, as an alternative to conventional polymer-based nanoparticles, offer great advantages in biomedical applications owing to their functional and biocompatible characteristics. However, the route of fabrication towards protein-based nanoparticles faces substantial challenges, including limitations in size control and unavoidable usage of toxic crosslinkers or organic solvents, which may raise safety concerns related to products and their degradation components. In the present study, a photo-induced crosslinking approach was developed to prepare stable, size-controlled protein-only nanoparticles. The facile one-step reaction irradiated by visible light enables the formation of monodispersed bovine serum albumin nanoparticles (BSA NPs) within several minutes through a tyrosine photo-redox reaction, requiring no cross-linking agents. The size of the BSA NPs could be precisely manipulated (from 20 to 100 nm) by controlling the duration time of illumination. The resultant BSA NPs exhibited spherical morphology, and the α-helix structure in BSA was preserved. Further study demonstrated that the 35 nm doxorubicin (DOX)-loaded BSA NPs achieved a drug loading content of 6.3%, encapsulation efficiency of 70.7%, and a controlled release profile with responsivity to both pH and reducing conditions. Importantly, the in vitro drug delivery experiment demonstrated efficient cellular internalizations of the DOX-loaded BSA NPs and inhibitory activities on MCF-7 and HeLa cells. This method shows the promise of being a platform for the green synthesis of protein-only nanoparticles for biomedical applications.
This paper references
10.1002/smll.201500550
Photosensitizer-Conjugated Albumin-Polypyrrole Nanoparticles for Imaging-Guided In Vivo Photodynamic/Photothermal Therapy.
Xuejiao Song (2015)
10.1021/acsami.7b19201
Self-Templated, Green-Synthetic, Size-Controlled Protein Nanoassembly as a Robust Nanoplatform for Biomedical Application.
Ya Wen (2018)
10.1016/j.biomaterials.2013.06.005
Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels.
Khoon S. Lim (2013)
10.1016/j.jconrel.2014.03.053
An overview of clinical and commercial impact of drug delivery systems.
Aaron C. Anselmo (2014)
10.3109/10717544.2013.801051
Biocompatibility, absorption and safety of protein nanoparticle-based delivery of doxorubicin through oral administration in rats
Kishore Golla (2013)
10.1002/adhm.201200285
One-step photo synthesis of protein-drug nanoassemblies for drug delivery.
Jinbing Xie (2013)
10.4314/AJB.V8I19.62445
Production of protein nanoparticles for food and drug delivery system.
M. Rahimnejad (2009)
10.1016/j.ijpharm.2010.03.063
Nanoparticles with dextran/chitosan shell and BSA/chitosan core--doxorubicin loading and delivery.
Jianing Qi (2010)
10.1016/j.jconrel.2011.09.069
Impact of albumin on drug delivery--new applications on the horizon.
B. Elsadek (2012)
10.1074/JBC.M102223200
Amyloid beta-protein oligomerization: prenucleation interactions revealed by photo-induced cross-linking of unmodified proteins.
G. Bitan (2001)
10.1016/j.biomaterials.2013.11.001
Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles.
Hong Jai Lee (2014)
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
Chemistry for the analysis of protein - protein interactions : Rapid and e ffi cient cross - linking triggered by long wavelength light ( vol 96 , pg 6020 , 1999 )
D. A. Fancy (2000)
10.1021/acs.biomac.8b00218
Translatable High Drug Loading Drug Delivery Systems Based on Biocompatible Polymer Nanocarriers.
W. Chen (2018)
10.1038/nbt876
Small-scale systems for in vivo drug delivery
David A. LaVan (2003)
10.1016/j.biomaterials.2008.12.059
The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant.
C. Elvin (2009)
10.1021/ACS.BIOCONJCHEM.6B00413
Rapid Covalent Immobilization of Proteins by Phenol-Based Photochemical Cross-Linking.
J. Ren (2016)
10.1016/J.NANO.2005.12.003
Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles.
C. Reis (2006)
10.1002/jps.23455
A novel self-assembly albumin nanocarrier for reducing doxorubicin-mediated cardiotoxicity.
A. Yuan (2013)
10.1002/jbm.a.32572
Evaluation of photo-crosslinked fibrinogen as a rapid and strong tissue adhesive.
C. Elvin (2010)
10.1002/BIP.20539
Nanogels prepared by self-assembly of oppositely charged globular proteins.
S. Yu (2006)
10.1088/1468-6996/11/1/014104
Biopolymeric nanoparticles
S. Sundar (2010)
10.1016/j.jconrel.2011.07.031
Albumin-based nanoparticles as potential controlled release drug delivery systems.
Ahmed O Elzoghby (2012)
10.1002/CHIN.201325247
Photo‐Induced Covalent Cross‐Linking for the Analysis of Biomolecular Interactions
G. W. Preston (2013)
10.1039/C4RA12802H
A self-assembled albumin based multiple drug delivery nanosystem to overcome multidrug resistance
Bin Chen (2015)
10.1098/rsif.2012.0740
Nanoscale assemblies and their biomedical applications
T. A. Doll (2013)
10.1073/PNAS.96.11.6020
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
D. Fancy (1999)
10.1016/S0378-5173(03)00134-0
Optimization of the preparation process for human serum albumin (HSA) nanoparticles.
K. Langer (2003)
10.1016/j.jconrel.2008.05.010
Albumin as a drug carrier: design of prodrugs, drug conjugates and nanoparticles.
F. Kratz (2008)
10.1016/j.biomaterials.2011.10.050
Doxorubicin-loaded human serum albumin nanoparticles surface-modified with TNF-related apoptosis-inducing ligand and transferrin for targeting multiple tumor types.
Sung-Ho Bae (2012)
10.1038/nrc.2016.108
Cancer nanomedicine: progress, challenges and opportunities
J. Shi (2017)
10.1039/C5TB01962A
Nano-assembly of bovine serum albumin driven by rare-earth-ion (Gd) biomineralization for highly efficient photodynamic therapy and tumor imaging.
Y. Wen (2016)
10.1021/JACS.6B06243
Nanoparticle Assembly of Surface-Modified Proteins.
Matthias Fach (2016)
10.1002/JBM.A.20090
Patterning adhesion of mammalian cells with visible light, tris(bipyridyl)ruthenium(II) chloride, and a digital micromirror array.
K. Luebke (2004)
10.1016/j.biomaterials.2013.10.028
The combined effects of size and surface chemistry on the accumulation of boronic acid-rich protein nanoparticles in tumors.
Jing Wang (2014)
10.1021/nn5062029
Nanoparticle-based immunotherapy for cancer.
Kun Shao (2015)



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