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CYTOTOXICITY AND DIFFERENTIATION EFFECTS OF GOLD NANOPARTICLES TO HUMAN BONE MARROW MESENCHYMAL STEM CELLS

J. Fan, W. Li, Wei-I Hung, Cheng-Pao Chen, J. Yeh
Published 2011 · Chemistry

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Gold nanoparticles (GNPs) are widely used in chemical sensing, drug delivery, biomedical imaging, and photothermal therapy due to their strong and size-tunable surface plasmon resonance, fluorescence, and easy-surface functionalization. In this study, we investigated the effects of water-dispersed GNPs on the cytotoxicity and differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the associated death pathway. The results showed that the viability of hBMSCs was dependent upon the size of GNPs. Further, GNPs at the smallest size exhibited the highest cytotoxicity after treatment for 5 days and also substantially suppressed the number of colony-forming unit-fibroblast (CFU-F) of hBMSCs after continuous exposure for 21 days. Although large and medium sizes of GNPs had minor cytotoxicity to the cells, the sizes of CFU-F formed in the groups treated with GNPs at medium and large sizes were smaller compared to the control group. Further study of the cell death pathway using GNPs at medium size found that GNPs triggered hBMSCs necrosis, possibly by oxidative stress after GNPs were endocytosed. In addition, GNPs exerted the inhibitory effects on induced osteogenesis and adipogenesis of hBMSCs. Alkaline phosphatase (ALP) activity and calcium mineralization during osteogenic induction as well as the accumulation of triacylglycerides in adipogenic hBMSCs were repressed significantly by coculturing with GNPs at medium size. Our results suggest that the application of GNPs as long-term tracers for the activities of mesenchymal stem cells (MSCs) should be carefully evaluated.
This paper references
10.1021/NL070363Y
Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes.
B. Chithrani (2007)
10.1002/smll.200900126
Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes.
Hung-Jen Yen (2009)
10.1002/SMLL.200700378
Size-dependent cytotoxicity of gold nanoparticles.
Y. Pan (2007)
CdSe Quantum Dots Induce Apoptosis via Activation of JNK and PAK2 in a Human Osteoblast Cell Line
H. Lu (2006)
10.1002/smll.200801546
Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects.
A. AlKilany (2009)
10.3390/ijms10052122
Cytotoxic Effects of CdSe Quantum Dots on Maturation of Mouse Oocytes, Fertilization, and Fetal Development
Ming-Shu Hsieh (2009)
10.1002/jat.1475
Cytotoxicity and biological effects of functional nanomaterials delivered to various cell lines
M. Mahmood (2010)
10.3109/10715760903271249
Enhancement of 5-Aminolevulinic acid-induced oxidative stress on two cancer cell lines by gold nanoparticles
S. Ito (2009)
10.1146/ANNUREV.BIOENG.5.011303.120723
Engineered nanomaterials for biophotonics applications: improving sensing, imaging, and therapeutics.
J. West (2003)
10.1021/LA0513712
Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview.
R. Shukla (2005)
10.1002/SMLL.200500492
Adverse effects of citrate/gold nanoparticles on human dermal fibroblasts.
N. Pernodet (2006)
10.1021/ac8004555
Assessment of cytotoxicity of quantum dots and gold nanoparticles using cell-based impedance spectroscopy.
K. Male (2008)
10.1016/j.toxlet.2010.05.003
Induction of cytotoxicity and apoptosis in mouse blastocysts by silver nanoparticles.
P. Li (2010)
10.1016/j.colsurfb.2008.07.004
Biodistribution of colloidal gold nanoparticles after intravenous administration: effect of particle size.
Ganeshchandra Sonavane (2008)
10.1016/j.tibtech.2005.12.004
Therapeutic possibilities of plasmonically heated gold nanoparticles.
D. Pissuwan (2006)
10.1002/SMLL.200400093
Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity.
E. E. Connor (2005)
10.25011/CIM.V31I3.3473
Enhanced radiation sensitivity in prostate cancer by gold-nanoparticles.
X. Zhang (2008)
10.1021/co100099r
Sequentially modified, polymer-stabilized gold nanoparticle libraries: convergent synthesis and aggregation behavior.
M. Gibson (2011)
10.1016/j.addr.2008.08.004
Quantum dots and nanoparticles for photodynamic and radiation therapies of cancer.
P. Juzenas (2008)
10.1016/j.bbrc.2010.02.046
Bioaccumulation and toxicity of gold nanoparticles after repeated administration in mice.
C. Lasagna-Reeves (2010)
10.1103/PHYSREVLETT.93.077402
Highly fluorescent, water-soluble, size-tunable gold quantum dots.
J. Zheng (2004)
10.1021/NL052396O
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells.
B. Chithrani (2006)
10.1186/1477-3155-2-12
Nanoparticles – known and unknown health risks
P. Hoet (2004)
10.1093/TOXSCI/KFI256
In vitro cytotoxicity of nanoparticles in mammalian germline stem cells.
L. Braydich-Stolle (2005)
10.1039/DF9511100055
A study of the nucleation and growth processes in the synthesis of colloidal gold
J. Turkevich (1951)
10.1016/j.biomaterials.2007.12.037
Particle size-dependent organ distribution of gold nanoparticles after intravenous administration.
W. D. de Jong (2008)
10.1182/BLOOD.V56.2.289.BLOODJOURNAL562289
Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny
H. Castro-Malaspina (1980)
10.1088/0957-4484/21/29/295101
Evaluation of cytotoxicity and radiation enhancement using 1.9 nm gold particles: potential application for cancer therapy.
K T Butterworth (2010)
10.1016/J.BIOMATERIALS.2005.09.004
The inhibition of osteogenesis with human bone marrow mesenchymal stem cells by CdSe/ZnS quantum dot labels.
S. Hsieh (2006)
10.1002/smll.200700794
Enhancement of radiation cytotoxicity in breast-cancer cells by localized attachment of gold nanoparticles.
T. Kong (2008)
10.1021/ar800035u
Gold nanoparticles in biology: beyond toxicity to cellular imaging.
C. Murphy (2008)
10.1186/1743-8977-6-18
Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441
Chiara Uboldi (2009)
10.1016/j.biomaterials.2010.06.051
Surface chemistry and aspect ratio mediated cellular uptake of Au nanorods.
Y. Qiu (2010)
10.2147/IJN.S8428
Toxicologic effects of gold nanoparticles in vivo by different administration routes
X. Zhang (2010)
10.1039/c0nr00478b
Surface charge of gold nanoparticles mediates mechanism of toxicity.
Nicole Schaeublin (2011)
10.1021/nn101373r
Gold nanoparticles promote osteogenic differentiation of mesenchymal stem cells through p38 MAPK pathway.
Changqing Yi (2010)
10.1038/sj.bjp.0707130
Nanoparticles: pharmacological and toxicological significance
C. Medina (2007)
10.1002/smll.200900466
Gold nanoparticles of diameter 1.4 nm trigger necrosis by oxidative stress and mitochondrial damage.
Y. Pan (2009)
10.1088/0957-4484/21/14/145101
In vitro toxicity studies of polymer-coated gold nanorods.
R. G. Rayavarapu (2010)
10.2174/138920009790274559
Nanotechology-based strategies to enhance the efficacy of photodynamic therapy for cancers.
W. Li (2009)



This paper is referenced by
10.3390/ijms19030754
Gold Nanoparticle-Induced Cell Death and Potential Applications in Nanomedicine
Hainan Sun (2018)
Marcação de células-tronco mesenquimais com nanopartículas metálicas funcionalizadas com ácido 2,3-Dimercaptosuccínico (DMSA)
Luisa H. A. Silva (2014)
10.21608/edj.2019.74794
Histological and Immuno-histochemical Study of the Cytotoxic Effects of Gold Nanoparticles on the Palate of Albino Rats
M. S. Youssef (2019)
10.15666/AEER/1605_57055714
FLAME ATOMIC ABSORPTION SPECTROMETRY DETERMINATION OF SILVER NANOPARTICLES IN ENVIRONMENTAL WATERS USING DISPERSIVE LIQUIDLIQUID MICROEXTRACTION
H. Wu (2018)
10.1002/jbm.b.34688
Functions and applications of metallic and metallic oxide nanoparticles in orthopedic implants and scaffolds.
Niyou Wang (2020)
Mechanisms of Nanoparticle Toxicity in Cancer and Normal Cells
Konstantina Tzelepi (2019)
10.1016/J.SAB.2016.04.009
Ultra-trace determination of gold nanoparticles in environmental water by surfactant assisted dispersive liquid liquid microextraction coupled with electrothermal vaporization-inductively coupled plasma-mass spectrometry
Y. Liu (2016)
10.1109/JSEN.2020.2997742
Surface Plasmon Resonance for Human Bone Marrow Cells Imaging
Thomas Wilkop (2020)
10.1186/s12915-020-0753-2
X-ray computed tomography in life sciences
S. Rawson (2020)
10.1002/jbm.a.35491
Shape and surface effects on the cytotoxicity of nanoparticles: Gold nanospheres versus gold nanostars.
P. Favi (2015)
10.1002/jat.3819
In vitro toxicity screening of magnetite nanoparticles by applying mesenchymal stem cells derived from human umbilical cord lining
T. Coccini (2019)
10.3390/ijms18020345
Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
P. L. Mok (2017)
10.1002/jbm.a.35518
Shape and surface chemistry effects on the cytotoxicity and cellular uptake of metallic nanorods and nanospheres.
P. Favi (2015)
10.1186/s12951-016-0213-x
Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications
Luisa H. A. Silva (2016)
10.1186/s13287-018-0954-6
The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells
C. Li (2018)
10.1016/j.aca.2012.11.050
Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry.
G. Hartmann (2013)
FLAME ATOMIC ABSORPTION SPECTROMETRY DETERMINATION OF SILVER NANOPARTICLES IN ENVIRONMENTAL WATERS USING DISPERSIVE LIQUID-LIQUID MICROEXTRACTION
(2018)
10.1007/s00253-020-10508-z
Gold nanoparticles biosynthesized by Nocardiopsis dassonvillei NCIM 5124 enhance osteogenesis in gingival mesenchymal stem cells
T. Bennur (2020)
10.1039/C3JA50342A
A novel strategy for sequential analysis of gold nanoparticles and gold ions in water samples by combining magnetic solid phase extraction with inductively coupled plasma mass spectrometry
Shaowei Su (2014)
10.1080/10408347.2018.1496010
Chromatographic Methods for the Determination of Emerging Contaminants in Natural Water and Wastewater Samples: A Review
M. T. García-Córcoles (2019)
10.1080/15376516.2019.1650151
In vitro evaluation of magnetite nanoparticles in human mesenchymal stem cells: comparison of different cytotoxicity assays
U. De Simone (2019)
10.1155/2017/6934757
Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells
N. Volkova (2017)
10.2147/IJN.S78775
Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
S. Choi (2015)
10.1021/acs.analchem.6b03532
Size-Based Analysis of Au NPs by Online Monolithic Capillary Microextraction-ICPMS.
Xiaolan Liu (2017)
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