Please confirm you are human (Sign Up for free to never see this)
← Back to Search
The Inflammatory Response After An Epidermal Burn Depends On The Activities Of Mouse Mast Cell Proteases 4 And 5
G. Younan, F. Suber, Wei Xing, T. Shi, Y. Kunori, M. Åbrink, G. Pejler, Susan M. Schlenner, hans-Reimer Rodewald, F. Moore, R. Stevens, R. Adachi, K. Austen, M. Gurish
Published 2010 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
A second-degree epidermal scald burn in mice elicits an inflammatory response mediated by natural IgM directed to nonmuscle myosin with complement activation that results in ulceration and scarring. We find that such burn injury is associated with early mast cell (MC) degranulation and is absent in WBB6F1-KitW/KitWv mice, which lack MCs in a context of other defects due to a mutation of the Kit receptor. To address further an MC role, we used transgenic strains with normal lineage development and a deficiency in a specific secretory granule component. Mouse strains lacking the MC-restricted chymase, mouse MC protease (mMCP)-4, or elastase, mMCP-5, show decreased injury after a second-degree scald burn, whereas mice lacking the MC-restricted tryptases, mMCP-6 and mMCP-7, or MC-specific carboxypeptidase A3 activity are not protected. Histologic sections showed some disruption of the epidermis at the scald site in the protected strains suggesting the possibility of topical reconstitution of full injury. Topical application of recombinant mMCP-5 or human neutrophil elastase to the scalded area increases epidermal injury with subsequent ulceration and scarring, both clinically and morphologically, in mMCP-5–deficient mice. Restoration of injury requires that topical administration of recombinant mMCP-5 occurs within the first hour postburn. Importantly, topical application of human MC chymase restores burn injury to scalded mMCP-4–deficient mice but not to mMCP-5–deficient mice revealing nonredundant actions for these two MC proteases in a model of innate inflammatory injury with remodeling.
This paper references
Mechanism for activation of mouse mast cell tryptase: dependence on heparin and acidic pH for formation of active tetramers of mouse mast cell protease 6.
J. Hallgren (2000)
Characterization of the mouse neutrophil elastase gene and localization to Chromosome 10
A. Belaaouaj (2009)
Mast cells of two types differing in neutral protease composition in the human aortic intima. Demonstration of tryptase- and tryptase/chymase-containing mast cells in normal intimas, fatty streaks, and the shoulder region of atheromas.
M. Kaartinen (1994)
Generation of C3a anaphylatoxin from human C3 by human mast cell tryptase.
L. Schwartz (1983)
The mouse mast cell-restricted tetramer-forming tryptases mouse mast cell protease 6 and mouse mast cell protease 7 are critical mediators in inflammatory arthritis.
H. McNeil (2008)
Deficiency of the tryptase-positive, chymase-negative mast cell type in gastrointestinal mucosa of patients with defective T lymphocyte function.
A. Irani (1987)
Rodent α‐chymases are elastase‐like proteases
Y. Kunori (2002)
Human Neutrophil Elastase-Mediated Cleavage Sites of MMP-9 and TIMP-1: Implications to Cystic Fibrosis Proteolytic Dysfunction
P. Jackson (2010)
Delayed Expulsion of the Nematode Trichinella spiralisIn Mice Lacking the Mucosal Mast Cell–Specific Granule Chymase, Mouse Mast Cell Protease-1
P. Knight (2000)
Protease–proteoglycan complexes of mouse and human mast cells and importance of their β‐tryptase–heparin complexes in inflammation and innate immunity
R. Stevens (2007)
Mast cell proteases.
R. Woodbury (1981)
Biological and molecular aspects of mast cell and basophil differentiation and function
北村 幸彦 (1995)
Mast cells play a pivotal role in ischaemia reperfusion injury to skeletal muscles
S. K. Bortolotto (2004)
Mast cell deficiency in KitW-sh mice does not impair antibody-mediated arthritis
J. Zhou (2007)
Angiotensin II generation by mast cell α- and β-chymases
G. Caughey (2000)
Transgenic mice that possess a disrupted mast cell protease 5 (MMCP-5) gene can not store carboxypeptidase a (MMC-CPA) protein in their granules
R. Stevens (1996)
Loss of Histochemical Identity in Mast Cells Lacking Carboxypeptidase A
T. Feyerabend (2005)
Mast Cells Contribute to Autoimmune Inflammatory Arthritis via Their Tryptase/Heparin Complexes1
Kichul Shin (2009)
Complement peptides C3a- and C5a-induced mediator release from dissociated human skin mast cells.
S. El-Lati (1994)
Mast Cell Protease 5 Mediates Ischemia-Reperfusion Injury of Mouse Skeletal Muscle1
J. P. Abonia (2005)
Long-range disruption of gene expression by a selectable marker cassette.
C. Pham (1996)
Transgenic mice that possess a disrupted mast cell protease 5 (mMCP-5) gene cannot store carboxypeptidase A in their granules
R. L. Stevens (1996)
A blockade of complement activation prevents rapid intestinal ischaemia‐reperfusion injury by modulating mucosal mast cell degranulation in rats
Attenuation of skeletal muscle reperfusion injury with intravenous 12 amino acid peptides that bind to pathogenic IgM.
R. Chan (2006)
The Mast Cell-restricted Tryptase mMCP-6 Has a Critical Immunoprotective Role in Bacterial Infections*
S. M. Thakurdas (2007)
Rodent alpha-chymases are elastase-like proteases.
Y. Kunori (2002)
Evidence for diversity of substrate specificity among members of the chymase family of serine proteases
S. Solivan (2002)
TGF-beta signal transduction.
J. Massagué (1998)
Mouse Mast Cell Tryptase mMCP-6 Is a Critical Link between Adaptive and Innate Immunity in the Chronic Phase of Trichinella spiralis Infection1
Kichul Shin (2008)
Carboxypeptidase A in mouse mast cells. Identification, characterization, and use as a differentiation marker.
W. E. Serafin (1987)
The Differing Roles of the Classical and Mannose-Binding Lectin Complement Pathways in the Events following Skeletal Muscle Ischemia-Reperfusion1
R. Chan (2006)
Angiotensin II generation by mast cell alpha- and beta-chymases.
G. Caughey (2000)
Reperfusion injury of ischemic skeletal muscle is mediated by natural antibody and complement
M. Weiser (1996)
Cloning and structural analysis of MMCP‐1, MMCP‐4 and MMCP‐5, three mouse mast cell‐specific serine proteases
R. Huang (1991)
Mast Cell Cathepsins C and S Control Levels of Carboxypeptidase A and the Chymase, Mouse Mast Cell Protease 5
F. Henningsson (2003)
Mast Cell Mediation of Muscle and Pulmonary Injury Following Hindlimb Ischemia–Reperfusion
C. Mukundan (2001)
The extended cleavage specificity of the rodent beta-chymases rMCP-1 and mMCP-4 reveal major functional similarities to the human mast cell chymase.
M. Andersson (2008)
Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases.
D. Reynolds (1990)
Extended Substrate Specificity of Rat Mast Cell Protease 5, a Rodent α-Chymase with Elastase-like Primary Specificity*
U. Karlson (2003)
Activation of rat serosal mast cells by chymase, an endogenous secretory granule protease.
B. Schick (1984)
Isolation and molecular cloning of mast cell carboxypeptidase A. A novel member of the carboxypeptidase gene family.
D. Reynolds (1989)
The Chymase, Mouse Mast Cell Protease 4, Constitutes the Major Chymotrypsin-like Activity in Peritoneum and Ear Tissue. A Role for Mouse Mast Cell Protease 4 in Thrombin Regulation and Fibronectin Turnover
E. Tchougounova (2003)
Heterogeneity of mast cells at multiple body sites. Fluorescent determination of avidin binding and immunofluorescent determination of chymase, tryptase, and carboxypeptidase content.
N. Weidner (1993)
A blockade of complement activation prevents rapid intestinal ischaemia-reperfusion injury by modulating mucosal mast cell degranulation in rats
木村 敏郎 (1998)
A Key Role for Mast Cell Chymase in the Activation of Pro-matrix Metalloprotease-9 and Pro-matrix Metalloprotease-2*
E. Tchougounova (2005)
Monoclonal antibody to intercellular adhesion molecule-1 reduces cardiac contractile dysfunction after burn injury in rabbits.
J. Horton (1996)
Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells.
H. McNeil (1991)
Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig.
H. McNeil (1992)
Alterations in leukocyte adhesion molecule expression after burn injury.
F. Nwariaku (1995)
Natural Disruption of the Mouse Mast Cell Protease 7 Gene in the C57BL/6 Mouse (*)
J. Hunt (1996)
Mouse mast cell proteases. In Biological and Molecular Aspects of Mast Cell and Basophil Differentiation and
J Hunt (1995)
Innate response to selfantigen significantly exacerbates burn wound
F. Suber (2007)
Mouse mast cell proteases
J. Hunt (1995)
Rat Mast Cell Protease 4 Is a β-Chymase with Unusually Stringent Substrate Recognition Profile*
U. Karlson (2002)
Species Differences in Angiotensin II Generation and Degradation by Mast Cell Chymases
Y. Kunori (2005)
Molecular mechanism of mast cell–mediated innate defense against endothelin and snake venom sarafotoxin
L. Schneider (2007)
Decreased neutrophils and megakaryocytes in anemic mice of genotype W/Wv
P. Chervenick (1969)
Innate response to self-antigen significantly exacerbates burn wound depth
F. Suber (2007)
Decrease of mast cells in W/Wv mice and their increase by bone marrow transplantation.
Y. Kitamura (1978)
Mouse mast cell proteases. In Biological and Molecular Aspects of Mast Cell and Basophil Differentiation and Function
J Hunt (1995)
A closely linked complex of mouse mast cell-specific chymase genes on chromosome 14.
M. Gurish (1993)
This paper is referenced by
Role of epidermal stem cells in repair of partial-thickness burn injury after using Moist Exposed Burn Ointment (MEBO(®)) histological and immunohistochemical study.
M. Elhadidy (2014)
A Review of the Evidence for and against a Role for Mast Cells in Cutaneous Scarring and Fibrosis
T. A. Wilgus (2020)
Preclinical assessment of the influence of inflammation on bone regeneration
D. Behrends (2017)
Mast cell secretory granules: armed for battle
S. Wernersson (2014)
Dual Targets for Mouse Mast Cell Protease-4 in Mediating Tissue Damage in Experimental Bullous Pemphigoid*
L. Lin (2011)
Mast cells limit extracellular levels of IL-13 via a serglycin proteoglycan-serine protease axis
I. Waern (2012)
Tight Junctions Epidermal Injury through Disruption of Mouse Mast Cell Proteases 4 and 5 Mediate
Michael F. Gurish (2014)
The Multifaceted Mast Cell in Inflammatory Bowel Disease
M. Hamilton (2014)
Mast cell proteases as pharmacological targets.
G. Caughey (2016)
Mast cell chymase reduces the toxicity of Gila monster venom, scorpion venom, and vasoactive intestinal polypeptide in mice.
Mitsuteru Akahoshi (2011)
Controlling Arteriogenesis and Mast Cells Are Central to Bioengineering Solutions for Critical Bone Defect Repair Using Allografts
B. Antebi (2016)
The TNF-α of mast cells induces pro-inflammatory responses during infection with Acinetobacter baumannii.
Takane Kikuchi-Ueda (2017)
Chapter 590 – Chymases
G. Caughey (2013)
Wounds, burns, trauma, and injury
M. Frieri (2016)
A Review of the Contribution of Mast Cells in Wound Healing: Involved Molecular and Cellular Mechanisms
Daniel Elieh Ali Komi (2019)
Mast cell activity in the healing wound: more than meets the eye?
Brian C. Wulff (2013)
Developmental origin and functional specialization of mast cell subsets.
M. Gurish (2012)
Role of mast cells in trauma and neuroinflammation in allergy immunology.
M. Frieri (2015)
Evidence That Mast Cells Are Not Required for Healing of Splinted Cutaneous Excisional Wounds in Mice
A. Nauta (2013)
Animal models in burn research
A. Abdullahi (2014)
Wound Healing: A Cellular Perspective.
M. Rodrigues (2019)
The Role of Mouse Mast Cell Proteases in the Proliferative Phase of Wound Healing in Microdeformational Wound Therapy
J. Succar (2014)
Peripheral Regulation of Pain and Itch
ELÍN INGIBJÖRG (2019)
Distorted Secretory Granule Composition in Mast Cells with Multiple Protease Deficiency
M. Grujić (2013)
Mouse Mast Cell Proteases 4 and 5 Mediate Epidermal Injury through Disruption of Tight Junctions
L. Bankova (2014)
Structura aparatului nervos al plexurilor coroide din ventriculele creierului uman
A. Darii (2013)
Expression profiling of constitutive mast cells reveals a uniqueidentity within the immune system
L. Lanier (2016)
The Role of the Inflammatory Response in Burn Injury
X. Strudwick (2017)
Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin
E. Magnúsdóttir (2018)
Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF-β1/Smads signaling pathway.
H. Chen (2017)
Mast Cell Modulation of the Tumor Microenvironment
Sharon A. Oldford (2013)
Neuropeptides, Inflammation, and Diabetic Wound Healing: Lessons from Experimental Models and Human Subjects
Ana Tellechea (2018)See more