Online citations, reference lists, and bibliographies.
← Back to Search

IL-8 Directly Enhanced Endothelial Cell Survival, Proliferation, And Matrix Metalloproteinases Production And Regulated Angiogenesis1

Ai-hua Li, S. Dubey, M. Varney, B. Dave, Rakesh K. Singh
Published 2003 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
IL-8, a member of the chemokine family, has been shown to play an important role in tumor growth, angiogenesis, and metastasis. The objective of this study was to determine the mechanism of IL-8-mediated angiogenesis. We examined the direct role of IL-8 in angiogenesis by examining IL-8 receptor expression on endothelial cells and their proliferation, survival, and matrix metalloproteinases (MMPs) production. We demonstrate that HUVEC and human dermal microvascular endothelial cells constitutively express CXCR1 and CXCR2 mRNA and protein. Recombinant human IL-8 induced endothelial cell proliferation and capillary tube organization while neutralization of IL-8 by anti-IL-8 Ab blocks IL-8-mediated capillary tube organization. Incubation of endothelial cells with IL-8 inhibited endothelial cell apoptosis and enhanced antiapoptotic gene expression. Endothelial cells incubated with IL-8 had higher levels of Bcl-xL:Bcl-xS and Bcl-2:Bax ratios. Furthermore, incubation of endothelial cells with IL-8 up-regulated MMP-2 and MMP-9 production and mRNA expression. Our data suggest that IL-8 directly enhanced endothelial cell proliferation, survival, and MMP expression in CXCR1- and CXCR2-expressing endothelial cells and regulated angiogenesis.
This paper references
10.1083/JCB.97.5.1648
In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices
R. Montesano (1983)
10.1016/0304-3835(87)90209-6
A simple quantitative assay for studying the invasive potential of high and low human metastatic variants.
M. J. Hendrix (1987)
Tumor cell motility.
L. Liotta (1991)
10.1126/SCIENCE.1840701
Structure and functional expression of a human interleukin-8 receptor.
W. Holmes (1991)
10.1126/SCIENCE.1281554
Interleukin-8 as a macrophage-derived mediator of angiogenesis.
A. Koch (1992)
10.1097/00008390-199209000-00006
Expression of cytokine genes, including IL‐6, in human malignant melanoma cell lines
M. Colombo (1992)
Interleukin-8 as a macrophagederived mediator of angiogenesis
A. E. Koch (1992)
10.1073/PNAS.90.9.4261
Expression of complete keratin filaments in mouse L cells augments cell migration and invasion.
Y. Chu (1993)
10.1016/0092-8674(93)90508-N
bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death
L. Boise (1993)
10.1016/0161-5890(93)90065-J
Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2.
D. Cerretti (1993)
Expression of interleukin 8 correlates with the metastatic potential of human melanoma cells in nude mice.
R. Singh (1994)
10.1084/JEM.179.5.1409
Inhibition of interleukin 8 attenuates angiogenesis in bronchogenic carcinoma
D. Smith (1994)
10.1016/0092-8674(94)90187-2
The implications of angiogenesis for the biology and therapy of cancer metastasis
I. Fidler (1994)
10.1038/NM0195-27
Angiogenesis in cancer, vascular, rheumatoid and other disease
J. Folkman (1995)
10.3892/IJO.6.1.119
Carcinoma-cells express IL-8 and the IL-8 receptor - their inhibition attenuates the growth of carcinoma-cells.
T. Ishiko (1995)
10.1006/CYTO.1995.0031
IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro.
P. Petzelbauer (1995)
10.1002/JLB.57.5.752
Role of C-X-C chemokines as regulators of angiogenesis in lung cancer.
R. Strieter (1995)
Retinoic acid induces cells cultured from oral squamous cell carcinomas to become anti-angiogenic.
M. Lingen (1996)
10.1074/JBC.272.18.11791
Progestins Prevent Apoptosis in a Rat Endometrial Cell Line and Increase the Ratio of bcl-X L tobcl-X S *
Adali Pecci (1997)
Expression of interleukin-8 by human melanoma cells up-regulates MMP-2 activity and increases tumor growth and metastasis.
M. Luca (1997)
10.1038/NM0897-917
A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma
G. Ambrosini (1997)
10.1146/ANNUREV.IMMUNOL.15.1.675
Human chemokines: an update.
M. Baggiolini (1997)
10.1053/GAST.1997.V113.PM9322516
Differential and regulated expression of C-X-C, C-C, and C-chemokines by human colon epithelial cells.
S. Yang (1997)
10.1038/25141
Control of apoptosis and mitotic spindle checkpoint by survivin
F. Li (1998)
10.1046/j.1365-2249.1998.00701.x
Modulation by proinflammatory cytokines of Fas/Fas ligand‐mediated apoptotic cell death of synovial cells in patients with rheumatoid arthritis (RA)
Wakisaka (1998)
10.1046/j.1365-2141.1998.00927.x
Patterns of cytokine expression in AIDS‐related non‐Hodgkin's lymphoma
Cristina Pastore (1998)
10.1016/S0022-1759(98)00128-8
Chemokines and their role in tumor growth and metastasis.
J. Wang (1998)
10.1172/JCI4461
Chemokines activate Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor in mammalian cells in culture.
M. Gershengorn (1998)
mokines activate Kaposi ’ s sarcoma - associated herpesvirus G protein - coupled receptor in mammalian cells in culture
P. Ghia G. Gaidano (1998)
10.1006/CYTO.1998.0465
Cxc chemokine receptor expression on human endothelial cells.
C. Murdoch (1999)
Clinico-biological implications of increased serum levels of interleukin-8 in B-cell chronic lymphocytic leukemia.
S. Molica (1999)
10.1007/978-1-4615-4737-2_36
Tumor cell motility.
T. Otto (1999)
10.1007/978-3-540-69184-6_9
The Bcl-2 protein family.
L. O’Connor (1999)
10.1006/EXCR.1999.4455
Escaping cell death: survival proteins in cancer.
M. Jäättelä (1999)
10.1016/S0002-9440(10)65284-4
Vascular endothelial growth factor (VEGF)-mediated angiogenesis is associated with enhanced endothelial cell survival and induction of Bcl-2 expression.
J. Nör (1999)
10.1074/jbc.274.16.11245
The Role of Antiapoptotic Bcl-2 Family Members in Endothelial Apoptosis Elucidated with Antisense Oligonucleotides*
E. Ackermann (1999)
10.1159/000015260
Assignment1 of the Aquaporin-8 water channel gene (AQP8) to human chromosome 16p12
L. Viggiano (1999)
10.1089/107999099313154
Interleukin-1β Upregulates MMP-9 Expression in Stromal Cells of Human Giant Cell Tumor of Bone
V. Rao (1999)
10.1038/sj.bmt.1701518
Immunoregulatory cytokines in bone marrow and peripheral blood stem cell products
R. Singh (1999)
10.1006/BBRC.1999.1589
Marked induction of the IAP family antiapoptotic proteins survivin and XIAP by VEGF in vascular endothelial cells.
J. Tran (1999)
Critical determinants of neoplastic angiogenesis.
I. Fidler (2000)
10.1038/35025220
Angiogenesis in cancer and other diseases
P. Carmeliet (2000)
Interleukin 8 expression regulates tumorigenicity and metastases in androgen-independent prostate cancer.
K. Inoue (2000)
10.1016/S1357-4310(00)01686-5
Matrix metalloproteinases: multifunctional contributors to tumor progression.
L. Mccawley (2000)
10.1182/blood.v96.1.34
Human endothelial cells express CCR2 and respond to MCP-1: direct role of MCP-1 in angiogenesis and tumor progression.
R. Salcedo (2000)
10.1096/fj.99-0963com
Differential expression and responsiveness of chemokine receptors (CXCR1–3) by human microvascular endothelial cells and umbilical vein endothelial cells
Rosalba Salcedo (2000)
10.1016/S0002-9440(10)64742-6
Control of apoptosis during angiogenesis by survivin expression in endothelial cells.
D. S. O'Connor (2000)
10.14670/HH-15.843
IL-8 expression in malignant melanoma: implications in growth and metastasis.
R. Singh (2000)
10.1038/74969
Chemotherapeutic drugs—more really is not better
I. Fidler (2000)
10.4049/jimmunol.165.9.5269
The CXC Chemokine Receptor 2, CXCR2, Is the Putative Receptor for ELR+ CXC Chemokine-Induced Angiogenic Activity1
C. Addison (2000)
10.1016/S0301-472X(00)00145-4
Immune dysfunction despite high levels of immunoregulatory cytokine gene expression in autologous peripheral blood stem cell transplanted non-Hodgkin's lymphoma patients.
R. Singh (2000)
10.1152/AJPLUNG.2001.280.6.L1094
IL-8 activates endothelial cell CXCR1 and CXCR2 through Rho and Rac signaling pathways.
I. Schraufstatter (2001)
10.1126/SCIENCE.1062892
What Does Radiotherapy Do to Endothelial Cells?
Judah Folkman (2001)
Expression of interleukin 8 and its receptors in human colon carcinoma cells with different metastatic potentials.
A. Li (2001)
Up-Regulation of Bcl-2 in microvascular endothelial cells enhances intratumoral angiogenesis and accelerates tumor growth.
J. Nör (2001)



This paper is referenced by
10.3390/ijms22116126
Pro- and Antiangiogenic Factors in Gliomas: Implications for Novel Therapeutic Possibilities
Magdalena Groblewska (2021)
10.3390/pharmaceutics13040495
Identification of Angiogenic Cargo in Extracellular Vesicles Secreted from Human Adipose Tissue-Derived Stem Cells and Induction of Angiogenesis In Vitro and In Vivo
P. Gangadaran (2021)
10.1093/carcin/bgab004
Measurement of 45 cytokine, chemokine and growth factors in established cell culture supernatants and autologous serum from advanced melanoma patients.
Y. G. Montoyo-Pujol (2021)
10.1007/s11694-021-00912-y
Anticancer, anticholinesterase and antidiabetic activities of tunceli garlic (Allium tuncelianum): determining its phytochemical content by LC–MS/MS analysis
K. Takım (2021)
10.1016/j.lfs.2021.119267
Apoptosis signal-regulating kinase 1 inhibition reverses deleterious indoxyl sulfate-mediated endothelial effects.
F. Savira (2021)
10.3390/ijms22168851
Iron–Quercetin Complex Preconditioning of Human Peripheral Blood Mononuclear Cells Accelerates Angiogenic and Fibroblast Migration: Implications for Wound Healing
Jiraporn Kantapan (2021)
10.1016/j.dental.2020.12.008
Influence of 2-hydroxyethyl methacrylate (HEMA) exposure on angiogenic differentiation of dental pulp stem cells (DPSCs).
André Jochums (2021)
10.3390/ijms22168877
Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation
Shao-Chun Wu (2021)
10.1101/2021.04.16.440131
Neuroprotection in early stages of Alzheimer’s Disease is promoted by Transthyretin angiogenic properties
Tiago Gião (2021)
10.1016/J.CEJ.2021.129487
Novel angiogenic metal nanoparticles controlling intracellular gene activation in stem cells
Gwang-Bum Im (2021)
10.3390/ani11082254
Current Status on Canine Foetal Fluid and Adnexa Derived Mesenchymal Stem Cells
E. Iacono (2021)
10.3390/ijms22147511
The Efficacy of Anti-PD-L1 Treatment in Melanoma Is Associated with the Expression of the ECM Molecule EMILIN2
Albina Fejza (2021)
10.3390/ijms22052634
The CXCL2/IL8/CXCR2 Pathway Is Relevant for Brain Tumor Malignancy and Endothelial Cell Function
Ruth M. Urbantat (2021)
10.1016/j.biomaterials.2021.120954
Delivery of a spheroids-incorporated human dermal fibroblast sheet increases angiogenesis and M2 polarization for wound healing.
Sung-Won Kim (2021)
10.1111/exd.14353
The effects of human dermal–derived mesenchymal stem cells on the keratinocyte proliferation and apoptosis in psoriasis
Wenjuan Chang (2021)
10.3390/cancers13092217
Low RECK Expression Is Part of the Cervical Carcinogenesis Mechanisms
Suellen Herbster (2021)
10.1016/B978-0-12-822527-1.00010-1
Intervention of IL-8-CXCR2 axis to reverse the resistance to GBM therapies
K. Angara (2021)
10.1093/biolre/ioab054
Role of inflammation in benign gynecologic disorders: from pathogenesis to novel therapies†
Abdelrahman Alashqar (2021)
10.1002/jctb.6601
Expansion of human mesenchymal stem/stromal cells on temporary liquid microcarriers
M. Hanga (2021)
10.1155/2021/6649608
Maternal Serum Cytokine Concentrations in Healthy Pregnancy and Preeclampsia
T. Spence (2021)
10.3389/fimmu.2021.670391
The Role of Cytokines in Predicting the Response and Adverse Events Related to Immune Checkpoint Inhibitors
Min Wang (2021)
10.1101/2021.09.01.21262833
Transcriptomics of acute DENV-specific CD8+ T cells does not support qualitative differences as drivers of disease severity.
A. Grifoni (2021)
10.1016/j.jcyt.2021.01.006
Thermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment.
Laura Frese (2021)
10.1080/2162402X.2021.1877459
Ectopic CD137 expression by rhabdomyosarcoma provides selection advantages but allows immunotherapeutic targeting
Kang yi Lee (2021)
10.1186/s13195-021-00883-8
Neuroprotection in early stages of Alzheimer’s disease is promoted by transthyretin angiogenic properties
Tiago Gião (2021)
10.3390/biom11040522
Alarming Cargo: The Role of Exosomes in Trauma-Induced Inflammation
Sarah A Walsh (2021)
10.1007/s11033-020-06104-z
Impaired capillary tube formation induced by elevated secretion of IL8 involves altered signaling via the CXCR1/PI3K/MMP2 pathway.
Y. Levy (2021)
10.1002/jat.4176
Tumors modulate fenestrated vascular beds and host endocrine status.
A. Hargreaves (2021)
10.1038/s41388-021-01758-w
CCL8 mediates crosstalk between endothelial colony forming cells and triple-negative breast cancer cells through IL-8, aggravating invasion and tumorigenicity
Eun-sook Kim (2021)
10.1016/j.ajpath.2021.01.002
Host Cxcr2-dependent regulation of pancreatic cancer growth, angiogenesis, and metastasis.
A. Purohit (2021)
10.3389/fmed.2021.643607
Angiocrine Regulation of Epithelial Barrier Integrity in Inflammatory Bowel Disease
M. Stürzl (2021)
10.3390/ijms22031355
The Stemness-High Human Colorectal Cancer Cells Promote Angiogenesis by Producing Higher Amounts of Angiogenic Cytokines via Activation of the Egfr/Akt/Nf-κB Pathway
Shin-Yi Chung (2021)
See more
Semantic Scholar Logo Some data provided by SemanticScholar