Characterization Of An Endophytic Bacterial Community Associated With Eucalyptus Spp.
R E L Procópio, Wagner L Araújo, Walter Maccheroni, João Lúcio Azevedo
Published 2009 · Medicine, Biology
Download PDFAnalyze on Scholarcy
Endophytic bacteria were isolated from stems of Eucalyptus spp (Eucalyptus citriodora, E. grandis, E. urophylla, E. camaldulensis, E. torelliana, E. pellita, and a hybrid of E. grandis and E. urophylla) cultivated at two sites; they were characterized by RAPD and amplified rDNA restriction analysis (ARDRA). Endophytic bacteria were more frequently isolated from E. grandis and E. pellita. The 76 isolates were identified by 16S rDNA sequencing as Erwinia/Pantoea (45%), Agrobacterium sp (21%), Curtobacterium sp (9%), Brevibacillus sp (8%), Pseudomonas sp (8%), Acinetobacter sp (4%), Burkholderia cepacia (2.6%), and Lactococcus lactis (2.6%). Genetic characterization of these endophytic bacteria isolates showed at least eight ARDRA haplotypes. The genetic diversity of 32 Erwinia/Pantoea and 16 Agrobacterium sp isolates was assessed with the RAPD technique. There was a high level of genetic polymorphism among all the isolates and there was positive correlation between the clusters and the geographic origin of the strains. These endophytic bacteria were further analyzed for in vitro interaction with endophytic fungi from Eucalyptus spp. We found that metabolites secreted by Erwinia/Pantoea and B. cepacia isolates had an inhibitory growth effect on some endophytic fungi, suggesting that these metabolites play a role in bacterial-fungal interactions inside the host plant. Apparently, these bacteria could have an important role in plant development; in the future they may be useful for biological control of diseases and plant growth promotion, as well as for the production of new metabolites and enzymes.
This paper references
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
A. V. Sturz (2000)
Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy.
A. Fiore (2001)
Control of Rhizoctonia solani and Sclerotium rolfsii in the greenhouse using endophytic Bacillus spp
eds.). Cambridge University Press, London, 205-222
NJ Phyllosphere Fokkema (1997)
Genotyping of bacteria belonging to the former Erwinia genus by PCR-RFLP analysis of a recA gene fragment.
M. Waleron (2002)
Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks.
W. L. Araújo (2001)
Genetic diversity and phylogeny of pink-pigmented facultative methylotrophic bacteria isolated from the phyllosphere of tropical crop plants
P. Raja (2008)
Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases.
J-H Yang (2007)
Xylella fastidiosa in citrus plants
JL Azevedo (2000)
WL Araújo (2002)
Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences.
S. Kwon (1997)
FUNGAL ENDOPHYTES: A Continuum of Interactions with Host Plants
K. Saikkonen (1998)
Endophytic microorganisms as potential growth promoters of banana
A. S. Y. Ting (2007)
Endophytic Bacterial Communities in Ginseng and their Antifungal Activity Against Pathogens
K. Cho (2007)
Diverse endophytic nitrogen-fixing bacteria isolated from wild rice Oryza rufipogon and description of Phytobacter diazotrophicus gen. nov. sp. nov.
Guo Xia Zhang (2007)
Interaction between endophytic bacteria from citrus plants and the phytopathogenic bacteria Xylella fastidiosa, causal agent of citrus-variegated chlorosis.
Paulo Teixeira Lacava (2004)
Winboot: A Program for Performing Bootstrap Analysis of Binary Data to Determine the Confidence Limits of UPGMA-based Dendrograms
I Yap (1996)
Bacterial Blight and Dieback of Eucalyptus Species, Hybrids, and Clones in South Africa.
T. Coutinho (2002)
Pseudomonads: major antagonistic endophytic bacteria to suppress bacterial wilt pathogen, Ralstonia solanacearum in the eggplant (Solanum melongena L.)
Raman Ramesh (2009)
Endophytic Bacteria and Their Potential Applications
C. Lodewyckx (2002)
Genetic diversity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China
J. H. Li (2008)
Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants
W. L. Araújo (2002)
Inoculation of Tree Roots with Plant Growth Promoting Soil Bacteria: An Emerging Technology for Reforestation
C. P. Chanway (1997)
Bacterial endophytes in agricultural crops
J. Hallmann (1997)
Fungal Endophytes of Tree Leaves
O. Petrini (1991)
Natural incidence of endophytic bacteria in pea cultivars under field conditions.
M. Elvira-Recuenco (2000)
biology of endophytism in plants with particular reference to woody perennials
G. Carroll (1986)
Chitinolytic activity of endophytic Streptomyces and potential for biocontrol.
Maria Carolina Quecine (2008)
Genetically modified bacteria in agriculture.
N. Amarger (2002)
Effect of host genotype on indigenous bacterial endophytes of cotton (Gossypium hirsutum L.)
P. D. Adams (2004)
Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion.
J. Kuklinsky-Sobral (2004)
Endophytic microorganisms: a review on insect control and recent advances on tropical plants
J. Azevedo (2000)
Endophytic bacteria in symptom-free cotton plants.
I. Misaghi (1990)
Diverse endophytic bacteria isolated from a leguminous tree Conzattia multiflora grown in Mexico
E. Wang (2006)
Diversity of endophytic enterobacteria associated with different host plants
A. R. Torres (2007)
Endophytic Bacterial Diversity in Rice (Oryza sativa L.) Roots Estimated by 16S rDNA Sequence Analysis
L. Sun (2007)
Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates
Rodrigo Teles Mendes (2007)
Mutualistic endophytic fungi and in-planta suppressiveness to plant parasitic nematodes
R. A. Sikora (2008)
This paper is referenced by
Population Variance and Diversity of Endophytic Fungi in Soybean (Glycine max (L) Merril)
Jm Dalal (2014)
Morphological and genetic characterization of endophytic bacteria isolated from roots of different maize genotypes
A. Ikeda (2012)
Beneficial microorganisms associated with sugarcane crops: the green gold for clean energy
Aline Silva Romão-Dumaresq (2017)
Endophytic Bacterium Pantoea Sugarcane Growth Promotion by the
Paulo Teixeira Lacava (2014)
DETEKSI SENYAWA METABOLIT SEKUNDER JAMUR ENDOFIT DARI AKAR TANAMAN KARET (Hevea brasiliensis)
Widya Lestari (2018)
Sugarcane Growth Promotion by the Endophytic Bacterium Pantoea agglomerans 33.1
M. C. Quecine (2012)
Endophytic Burkholderia fungorum DBT1 can improve phytoremediation efficiency of polycyclic aromatic hydrocarbons.
Marco Andreolli (2013)
Isolation, bioprospecting and diversity of salt-tolerant bacteria associated with sugarcane in soils of Pernambuco, Brazil
Maria Camila de Barros Silva Leite (2014)
Pantoea agglomerans Influence on the State and Activity of Cereals Photosynthetic Apparatus
Hanna Huliaieva (2020)
Ralstonia solanacearum and R. pseudosolanacearum on Eucalyptus: Opportunists or Primary Pathogens?
Teresa Coutinho (2017)
MICR N D 0 N Cloning and Expression of AnsZ Gene Encoding L-Asparaginase from Local Bacillus subtilis Diversity of Lactic Acid Bacteria Isolated from Indonesian Traditional Fermented Foods
Rifa Sulistiyani ()
From Genome to Phenotype: An Integrative Approach to Evaluate the Biodiversity of Lactococcus lactis
Valérie Laroute (2017)
Operational taxonomic units (OTUs) of endophytic bacteria isolated from banana cultivars in the Amazon.
Ávila Souza (2015)
Endophytes of Forest Trees
Anna Maria Pirttilä (2011)
Endophytic Microorganisms in Leaves of Moringa oleifera Collected in Three Localities at Pernambuco State, Northeastern Brazil
Igor Felipe Andrade Costa de Souza (2016)
The Microbiome of Eucalyptus Roots under Different Management Conditions and Its Potential for Biological Nitrogen Fixation
Eduardo J S Fonseca (2017)
Genetic diversity of Burkholderia (Proteobacteria) species from the Caatinga and Atlantic rainforest biomes in Bahia, Brazil.
Ana Carolina Monteiro Santini (2013)
Biological control of fusarium wilt of pine seedlings using endophytic microorganisms and silicon.
Bomikazi Nobesuthu. Gqola (2015)
Significant host jump of Xanthomonas vasicola from sugarcane to a Eucalyptus grandis clone in South Africa
Teresa Coutinho (2015)
Reviving of the endophytic bacterial community as a putative mechanism of plant resistance
Olga Podolich (2014)
Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae).
Sandro Augusto Rhoden (2015)
Green synthesis of silver nanoparticles using Bacillus cereus, an endophytic bacterium isolated from Garcinia xanthochymus
Swetha Sunkar (2011)
Selection and characterization of endophytic bacteria as biocontrol agents of tomato bacterial wilt disease
Abdjad Asih Nawangsih (2011)
Biodecaffeination by endophytic Pseudomonas sp. isolated from Coffee arabica L
Syed Baker (2012)
Assessment of filamentous fungal diversity using classic and molecular approaches: case study – Mediterranean ecosystem
Júlia Berger Guimarães (2011)
MICR N D 0 N
Rima Azara (2014)
Diversity and distribution of the endophytic bacterial community at different stages of Eucalyptus growth
Paulo Sérgio Balbino Miguel (2016)
Population Dynamics and Diversity of Endophytic Bacteria Associated with Soybean (Glycine max (L) Merril)
Jitendra Dalal (2013)
Seasonal variation of microflora and their effects on the quality of wood chips intended for pulping.
L Govender (2013)
Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems
Catiusca Reali (2017)
Temporal variations and organ distribution of endophytic microorganisms associated with woody plants
Thongsandee Watchararuethai (2012)
Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum
Erick Francisco S. Rakotoniriana (2012)See more