Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Poly(Ethylene Glycol) And Hydroxy Functionalized Alkane Phosphate Self-Assembled Monolayers Reduce Bacterial Adhesion And Support Osteoblast Proliferation

S. Bozzini, P. Petrini, M. C. Tanzi, C. R. Arciola, S. Tosatti, L. Visai
Published 2011 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Purpose Presently there is interest today in designing improved titanium surfaces capable of high bioactivity in order to promote strong anchorage of the bone surrounding implants while at the same time discouraging bioadhesion. Poly(ethylene glycol)-modified (PEG) alkane phosphate and OH-terminated alkane phosphates have been demonstrated to be spontaneously adsorbed onto titanium oxide surfaces and produce surfaces with different protein resistance in relation to the PEG surface density. This study aims to evaluate caries-associated Streptococcus mutans (S. mutans) adhesion and osteoblast proliferation while varying the PEG surface density of titanium surfaces. Methods Bacterial adhesion was quantified by fluorescence microscopy and SAOS-2 human osteoblast proliferation was evaluated up to 7 days of culture in vitro. Metabolic activity of osteoblasts was measured by MTT test and the secretion of extracellular matrix proteins (osteopontin, osteocalcin and type I collagen) in culture medium was determined by immunoenzymatic assays. Results As the PEG surface density increased, the bacterial adhesion considerably decreased when compared to uncoated titanium surfaces. The monomolecular coatings proved to be capable of supporting osteoblast proliferation with the greatest levels of metabolic activity at the highest PEG surface concentrations. Conclusions These results are extremely promising for potential clinical application in implant uses where both reduction of bacteria adhesion and stimulation of bone formation are highly desirable.
This paper references
10.1002/jbm.a.32932
Effects of electrodeposited poly(ethylene glycol) on biofilm adherence to titanium.
Y. Tanaka (2010)
10.1177/039139880903200915
Novel Surface Coatings Modulating Eukaryotic Cell Adhesion and Preventing Implant Infection
J. Groll (2009)
10.1016/j.biomaterials.2009.12.029
Direct and indirect effects of microstructured titanium substrates on the induction of mesenchymal stem cell differentiation towards the osteoblast lineage.
R. Olivares-Navarrete (2010)
10.1016/S0142-9612(97)00245-7
Bacterial adhesion on PEG modified polyurethane surfaces.
K. Park (1998)
10.1016/S0039-6028(01)01548-5
The role of surface science in bioengineered materials
M. Tirrell (2002)
10.1021/LA000736+
Poly(l-lysine)-g-poly(ethylene glycol) Layers on Metal Oxide Surfaces: Surface-Analytical Characterization and Resistance to Serum and Fibrinogen Adsorption
Ning-Ping Huang (2001)
10.1021/LA000818Y
Force Measurements between Bacteria and Poly(ethylene glycol)-Coated Surfaces
Anneta Razatos (2000)
10.1016/J.BIOMATERIALS.2003.11.033
Staphylococcus aureus adhesion to titanium oxide surfaces coated with non-functionalized and peptide-functionalized poly(L-lysine)-grafted-poly(ethylene glycol) copolymers.
L. Harris (2004)
10.1021/LA050386X
Relationship between interfacial forces measured by colloid-probe atomic force microscopy and protein resistance of poly(ethylene glycol)-grafted poly(L-lysine) adlayers on niobia surfaces.
S. Pasche (2005)
10.1016/j.biomaterials.2010.10.011
Competitive time- and density-dependent adhesion of staphylococci and osteoblasts on crosslinked poly(ethylene glycol)-based polymer coatings in co-culture flow chambers.
Isabel C. Saldarriaga Fernández (2011)
10.1016/S0142-9612(01)00334-9
Effects of cloud-point grafting, chain length, and density of PEG layers on competitive adsorption of ocular proteins.
P. Kingshott (2002)
10.1016/J.DENTAL.2006.06.025
Surface treatments of titanium dental implants for rapid osseointegration.
Laurent Le Guéhennec (2007)
10.22203/ECM.V006A03
Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies.
B. Boyan (2003)
10.1016/J.BIOMATERIALS.2006.03.009
Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence: a cell morphology study.
M. Schuler (2006)
10.1007/978-1-4899-0703-5
Poly(Ethylene Glycol) Chemistry
J. M. Harris (1992)
10.1016/J.BIOMATERIALS.2006.02.022
Integrin beta1 silencing in osteoblasts alters substrate-dependent responses to 1,25-dihydroxy vitamin D3.
L. Wang (2006)
10.1016/J.BIOMATERIALS.2007.02.024
Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography.
G. Zhao (2007)
10.1177/228080000300100201
Osteointegration of Titanium and Its Alloys by Anodic Spark Deposition and other Electrochemical Techniques: A Review
R. Chiesa (2003)
10.1099/00221287-134-5-1299
Adherence of streptococci to surface-modified glass.
N. Satou (1988)
10.1016/j.biomaterials.2008.05.012
Advancing dental implant surface technology--from micron- to nanotopography.
G. Mendonça (2008)
10.1021/LA034111Y
Poly(l-lysine)-graft-poly(ethylene glycol) Assembled Monolayers on Niobium Oxide Surfaces: A Quantitative Study of the Influence of Polymer Interfacial Architecture on Resistance to Protein Adsorption by ToF-SIMS and in Situ OWLS
S. Pasche (2003)
10.1021/JP050431+
Effects of ionic strength and surface charge on protein adsorption at PEGylated surfaces.
S. Pasche (2005)
10.1002/chem.200801134
Protein-resistant surfaces through mild dopamine surface functionalization.
J. Wach (2008)
10.1021/la904066y
Poly(ethylene glycol) and hydroxy functionalized alkane phosphate mixed self-assembled monolayers to control nonspecific adsorption of proteins on titanium oxide surfaces.
S. Bozzini (2010)
Surface treatments of titanium dental implants for rapid osseointegration
L LeGuéhennec (2007)
10.1016/j.biomaterials.2011.01.029
The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation.
R. Gittens (2011)
10.1021/MA9020664
Surface Assembly of Catechol-Functionalized Poly(l-lysine)-graft-poly(ethylene glycol) Copolymer on Titanium Exploiting Combined Electrostatically Driven Self-Organization and Biomimetic Strong Adhesion
S. Saxer (2010)
10.1006/JCIS.1998.5513
Effect of chain density on inhibition of protein adsorption by poly(ethylene glycol) based coatings
Martin Malmsten (1998)
10.1021/JA056256S
Biomimetic surface modifications based on the cyanobacterial iron chelator anachelin.
S. Zürcher (2006)
10.1002/JBM.A.30320
High surface energy enhances cell response to titanium substrate microstructure.
G. Zhao (2005)
10.1023/A:1008913915399
Effect of hydrophobicity on in vitro streptococcal adhesion to dental alloys
M. Grivet (2000)
10.1177/039139880903200915
Novel Surface Coatings Modulating Eukaryotic Cell Adhesion and Preventing Implant Infection
Juergen Groll (2009)
10.1016/J.POLYMER.2010.08.022
Surface Hydration: Principles and Applications Toward Low-fouling/nonfouling Biomaterials
Shenfu Chen (2010)
10.1016/j.biomaterials.2010.08.031
Staphylococcus aureus resistance on titanium coated with multivalent PEGylated-peptides.
X. Khoo (2010)
10.1016/J.BIOMATERIALS.2004.04.020
Synergistic interaction of topographic features in the production of bone-like nodules on Ti surfaces by rat osteoblasts.
M. Wieland (2005)
10.1007/978-1-4899-0703-5_1
Introduction to Biotechnical and Biomedical Applications of Poly(Ethylene Glycol)
J. M. Harris (1992)
10.1016/S1359-0286(99)00018-2
Surfaces that resist bioadhesion
P. Kingshott (1999)
10.1016/S0006-3495(97)78698-3
Protein adsorption on surfaces with grafted polymers: a theoretical approach.
I. Szleifer (1997)
10.1021/JP9716952
MOLECULAR CONFORMATION AND SOLVATION OF OLIGO(ETHYLENE GLYCOL)-TERMINATED SELF-ASSEMBLED MONOLAYERS AND THEIR RESISTANCE TO PROTEIN ADSORPTION
R. Wang (1997)
10.1021/LA048626G
Protein resistance of titanium oxide surfaces modified by biologically inspired mPEG-DOPA.
J. Dalsin (2005)
10.1201/9780203491430.sec5
How Surfaces Interact with the Biological Environment
P. Tengvall (2003)
10.1021/ja202760x
Self-assembly of focal point oligo-catechol ethylene glycol dendrons on titanium oxide surfaces: adsorption kinetics, surface characterization, and nonfouling properties.
Torben Gillich (2011)
10.1021/LA051726H
Influence of PEG architecture on protein adsorption and conformation.
R. Michel (2005)
10.1163/156856200743869
On the molecular basis of fouling resistance
M. Morra (2000)
10.1021/am900337z
Adsorption and lubricating properties of poly(l-lysine)-graft-poly(ethylene glycol) on human-hair surfaces.
S. Lee (2009)
10.1002/jbm.a.32596
The role of phospholipase D in osteoblast response to titanium surface microstructure.
Mimi Fang (2010)
10.1111/J.1574-6968.1996.TB08408.X
Attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment.
L. Ista (1996)
10.1002/JBM.A.20082
RGD-containing peptide GCRGYGRGDSPG reduces enhancement of osteoblast differentiation by poly(L-lysine)-graft-poly(ethylene glycol)-coated titanium surfaces.
S. Tosatti (2004)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar