Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Fluorescence Detection Based Point-of-care Diagnostics Platforms : Bridging The Gap Between Laboratory And Market

N. Adsul
Published 2014 · Engineering

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Point-of-Care (PoC) diagnostics delivers optimal healthcare by providing rapid results at lower cost than the corresponding laboratory tests. It’s applications extend beyond healthcare to environmental monitoring for testing of pollutants in the field, tuning athletic performance by testing biochemical parameters directly at the sports facility, agricultural monitoring for plant and animal disease control and even homeland security and social welfare for detection of substance of abuse, explosives. Although such promising applications exist, only a small fraction of PoC type sensor platforms progress from laboratory to the market. It is important to develop sensing platforms keeping in mind the boundaries of technology and cost with respect to the point of care usage. During system integration, it is extremely crucial to consider limitations with respect to size, weight, power, time to result and most importantly cost per unit earlier in the development phase. Instead of solving all problems into a single system, as traditionally done, we followed a diverse and functional approach. Three different system platforms are developed. Each one tackles specific combinations of challenges. This provides a benchmark in terms of performance, cost for each technology integration approach within given set of boundary conditions. First platform demonstrates novel in-coupling of the excitation light from a photoluminescent polymer into a low mode optical waveguide without the use of exotic coupling techniques such as gratings, prism, end face or butt coupling. Second platform integrates mechanical support, microfluidics and optical in-coupling structures into a single plastic substrate using hot embossing technique. Optical diffraction gratings are embossed on one side and simultaneously microfluidics is embossed on the opposite side. The total internal reflection path of the in-coupled light is designed using developed model so that a reflection occurs at the position of the sensing region. We designed, optimized and fabricated the chips for performing in-situ fluorescent immunoassay. Fluorescently labelled antibodies captured on the sensing surface can be photo-excited by evanescent field for surface sensitive detection and live measurements. For end result type of measurements, through excitation with off normal illumination can be chosen. These two different excitation schemes can be selected simply by changing the incident angle. Third platform is developed to be a fully disposable single package for simultaneous detection of three cardiac markers namely CK-MB, Troponin-I and Myoglobin. We demonstrated unique capabilities of organic field effect transistors (FET) as light generation and detection devices. Microfluidics and all optical components such as polarizers, filters are integrated on the disposable chip by lamination. Trans-illumination excitation is used and light sources are low cost inorganic green LEDs or organic light emitters such as OLEDs or Organic Light Emitti¬ng-FETs. Detectors used are silicon photodiodes or organic light sensitive devices such as OPDs or Organic Light Sensing FETs. This system is being further commercially developed system for company Molecular Vision Ltd. We benchmarked all three platforms using fluorescence immunoassay and compared with existing commercial system with respect to cost and performance. We used off the shelf lower cost components in the design taking into account final cost considerations but at the same time testing the limits of performance. Our work has developed niche set of skills and a mind-set vital to establish “Design for Point-of-Care” philosophy.
This paper references
10.1039/c003477k
Lensfree microscopy on a cellphone.
D. Tseng (2010)
10.1038/nmat2751
Organic light-emitting transistors with an efficiency that outperforms the equivalent light-emitting diodes.
R. Capelli (2010)
10.1002/1522-2683(200110)22:18<3939::AID-ELPS3939>3.0.CO;2-9
Single molecule detection of double‐stranded DNA in poly(methylmethacrylate) and polycarbonate microfluidic devices
M. Wabuyele (2001)
10.1039/c2lc40235a
Integrated rapid-diagnostic-test reader platform on a cellphone.
O. Mudanyali (2012)
10.1080/10408349508050556
Glucose Oxidase as an Analytical Reagent
J. Raba (1995)
10.1002/PSSC.201000494
Fabrication and characteristics of ambipolar, light‐emitting organic field effect transistors utilizing blended polyfluorene derivatives
Kyohei Koiwai (2011)
10.1117/12.668745
Organic thin films based sensor applications
Soyoun Jung (2006)
10.1364/JOSA.69.001495
Light emission by magnetic and electric dipoles close to a plane dielectric interface. III. Radiation patterns of dipoles with arbitrary orientation
W. Lukosz (1979)
10.1021/AC991358K
Forbidden light detection from single molecules
Ruckstuhl (2000)
10.1063/1.3509405
Mechanically tunable conjugated polymer distributed feedback lasers
Bernard Wenger (2010)
10.1109/T-ED.1985.22096
Remote fiber-optic biosensors based on evanescent-excited fluoro-immunoassay: Concept and progress
J. D. Andrade (1985)
10.1039/c3lc50785h
Albumin testing in urine using a smart-phone.
A. Coskun (2013)
10.1364/OE.17.005075
Evanescent-wave fluorescence microscopy using symmetric planar waveguides.
B. Agnarsson (2009)
10.2144/99263PF02
PBXL-1: a new fluorochrome applied to detection of proteins on membranes.
J. Morseman (1999)
10.1016/J.SNB.2008.08.027
An optical PMMA biochip based on fluorescence anisotropy: Application to C-reactive protein assay
F. Baldini (2009)
10.1364/JOSA.71.000744
Light emission by multipole sources in thin layers. I. Radiation patterns of electric and magnetic dipoles
W. Lukosz (1981)
10.1063/1.2809878
Fundamentals of Photonics
B. Saleh (1991)
Polysaccharides for functional biomolecule display on surfaces
Norbert Sprenger (2005)
The path to ubiquitous and low-cost organic electronic appliances on
Stephen R Forrest (2004)
10.1038/NMAT1699
A bright future for organic field-effect transistors
M. Muccini (2006)
10.1364/JOSA.67.001607
Light emission by magnetic and electric dipoles close to a plane interface. I. Total radiated power
W. Lukosz (1977)
10.1039/b916888e
Advanced printing and deposition methodologies for the fabrication of biosensors and biodevices.
L. Gonzalez-Macia (2010)
10.1117/12.831579
Efficiency of fluorescence coupling into planar waveguides
Ronja Bäumner (2009)
10.1016/J.ORGEL.2006.01.002
Photoresponse of organic field-effect transistors based on conjugated polymer/fullerene blends
N. Marjanović (2005)
10.1021/ac9026109
A planar, chip-based, dual-beam refractometer using an integrated organic light-emitting diode (OLED) light source and organic photovoltaic (OPV) detectors.
Erin L. Ratcliff (2010)
10.1039/c2lc21204h
Commercialization of microfluidic point-of-care diagnostic devices.
C. Chin (2012)
10.1039/c3lc50589h
Smart-phone based computational microscopy using multi-frame contact imaging on a fiber-optic array.
Isa Navruz (2013)
10.1039/B504551G
Towards microalbuminuria determination on a disposable diagnostic microchip with integrated fluorescence detection based on thin-film organic light emitting diodes.
O. Hofmann (2005)
10.1021/ac8016289
Perspective on optical biosensors and integrated sensor systems.
F. Ligler (2009)
10.1002/POLB.22111
Optical biosensors based on integrated polymer light source and polymer photodiode
M. Ramuz (2011)
10.1039/c3lc41408f
Cost-effective and rapid blood analysis on a cell-phone.
H. Zhu (2013)
10.1039/b914183a
A packaged optical slot-waveguide ring resonator sensor array for multiplex label-free assays in labs-on-chips.
C. F. Carlborg (2010)
10.1016/J.SNB.2004.10.005
Thin-film organic photodiodes as integrated detectors for microscale chemiluminescence assays
O. Hofmann (2005)
10.1063/1.2061892
Highly sensitive thin-film organic phototransistors: Effect of wavelength of light source on device performance
Yong-Young Noh (2005)
10.1016/0956-5663(91)80007-K
Output grating couplers on planar optical waveguides as direct immunosensors.
W. Lukosz (1991)
10.1016/0030-4018(77)90331-5
Fluorescence lifetime of magnetic and electric dipoles near a dielectric interface
W. Lukosz (1977)
10.1021/ar100066t
Perfluorophenyl azides: new applications in surface functionalization and nanomaterial synthesis.
L. Liu (2010)
10.1007/s00216-010-4165-y
Ratiometric fluorescence-based dissolved carbon dioxide sensor for use in environmental monitoring applications
D. Wencel (2010)
10.1016/S0925-3467(97)00082-7
Stability of polymer LEDs
A. Berntsen (1998)
10.1364/JOSAB.4.000337
Fluorescence emission at dielectric and metal-film interfaces
E. Hellen (1987)
10.1002/1615-9861(200204)2:4<383::AID-PROT383>3.0.CO;2-E
Zeptosens' protein microarrays: A novel high performance microarray platform for low abundance protein analysis
M. Pawlak (2002)
10.1063/1.3097276
Coupling light from an organic light emitting diode (OLED) into a single-mode waveguide: Toward monolithically integrated optical sensors
M. Ramuz (2009)
10.1016/J.APSUSC.2008.04.023
Optical inhomogeneity of ZnS films deposited by thermal evaporation
Xiaochun Wu (2008)
10.1039/c2lc41152k
A personalized food allergen testing platform on a cellphone.
A. Coskun (2013)
10.1023/A:1022503600141
PBXL Fluorescent Dyes for Ultrasensitive Direct Detection
S. J. Zoha (2004)
10.5-in. VGA all-printed flexible organic TFT backplane for electrophoretic displays. In IDW ‘07: PROCEEDINGS OF THE 14TH INTERNATIONAL DISPLAY WORKSHOPS
T Okubo (2007)
10.1039/B611067C
Integrated thin-film polymer/fullerene photodetectors for on-chip microfluidic chemiluminescence detection.
Xuhua Wang (2007)
10.1016/S0003-2670(01)01593-8
Planar waveguides for ultra-high sensitivity of the analysis of nucleic acids
G. Duveneck (2002)
10.1039/c2lc40741h
Point-of-care colorimetric detection with a smartphone.
L. Shen (2012)
10.1007/s10895-011-0909-0
Enhanced Fluorescence Anisotropy Assay for Human Cardiac Troponin I and T Detection
Yanling Qiao (2011)
10.1021/ac8027323
Organic photodiodes for biosensor miniaturization.
Jason Wojciechowski (2009)
10.1039/b906523g
Toward one-step point-of-care immunodiagnostics using capillary-driven microfluidics and PDMS substrates.
L. Gervais (2009)
10.1109/PROC.1985.13220
Analysis and applications of optical diffraction by gratings
T.K. Gaylord (1985)
10.1002/ADMA.201190098
Microfluidic Diagnostic Devices: Microfluidic Chips for Point‐of‐Care Immunodiagnostics (Adv. Mater. 24/2011)
L. Gervais (2011)
10.3390/s130607872
In situ Measurements of Phytoplankton Fluorescence Using Low Cost Electronics
Thomas Leeuw (2013)
10.1039/B508288A
The autofluorescence of plastic materials and chips measured under laser irradiation.
A. Piruska (2005)
10.3390/s120202324
A Bioanalytical Platform for Simultaneous Detection and Quantification of Biological Toxins
O. Weingart (2012)
10.1016/0022-1759(75)90116-7
A new immunoassay based on fluorescence excitation by internal reflection spectroscopy.
M. Kronick (1975)
10.1039/B909366B
Nanofluidics, from bulk to interfaces.
L. Bocquet (2010)
10.1007/s00216-008-2424-y
Rapid test strips for analysis of mycotoxins in food and feed
R. Krska (2009)
10.1016/J.SNA.2006.11.038
Design and fabrication of polymer microfluidic substrates using the optical disc process
Chun-Han Wu (2007)
10.1017/CBO9780511781797
Fundamentals of Micro-Optics
H. Zappe (2010)
10.1039/c0lc00586j
Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip.
Gihan Ryu (2011)
10.1002/ADMA.200501152
Device Physics of Solution‐Processed Organic Field‐Effect Transistors
H. Sirringhaus (2005)
10.1021/nn4037706
Fluorescent imaging of single nanoparticles and viruses on a smart phone.
Q. Wei (2013)
10.1039/B313503A
Thin-film polymer light emitting diodes as integrated excitation sources for microscale capillary electrophoresis.
J. Edel (2004)
10.1016/c2010-0-66244-4
The Immunoassay Handbook
D. Wild (2001)
10.1016/J.SSE.2009.10.010
Plastic circuits and tags for 13.56 MHz radio-frequency communication
K. Myny (2009)
10.1016/s1369-7021(07)70020-2
Principles of nano-optics
L. Novotny (2006)
10.1364/OL.15.001446
High-efficiency single-order waveguide grating coupler.
I. Avrutsky (1990)
10.1021/ac1032758
Supercritical angle fluorescence immunoassay platform.
T. Ruckstuhl (2011)
10.1039/B603678C
Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection.
O. Hofmann (2006)
10.1039/B307772C
Nonlinear decrease of background fluorescence in polymer thin-films - a survey of materials and how they can complicate fluorescence detection in microTAS.
K. Hawkins (2003)
10.1063/1.2457801
Photovoltaic effects on the organic ambipolar field-effect transistors
S. Cho (2007)
10.1364/JOSA.67.001615
Light emission by magnetic and electric dipoles close to a plane dielectric interface. II. Radiation patterns of perpendicular oriented dipoles
W. Lukosz (1977)
Integration of organic optoelectronic
Marc Ramuz (2009)
10.1039/b914635k
Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin.
T. Mairal (2009)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar